After alkaine hydrolysis, the body wall of Slicliopus varicyatus was fractionally pre-cipitatecd with alcohol, then oxidatively decoloured and purified with KAc, the elcctrophorelically pure polysaccharides SVI and SVIIwere obtained. The results of ultracentrifugal analysis showed that the sedimentation coefficients were S20w 3. 09 for SVI and S20w 2. 12 for SVII. The metachromatic reaction with reddish black I showed the metachromatic peaks of SVI and SVII were shifted to 514nm and 507nm, respectively. The molecule of SVI is composed of aminogalactose, glucuronic acid, fucose and sulfate (1:1. 21:1. 29:4. 62) and that of SVII is composed of fucose and sulfate (1.0. 99). And our results of Stichopus japonicus acidic mucopolysaccharide ultracentrifugation therefore suggests that the sedimentation coefficients is S20w 2. 12. The metachromatic reaction with reddish black I showed the metachromatic peak is 515nm. The molecule of Sjamp is also composed of aminogalactose, glucuronic acid, fucose and sulfate ( 1:1. 1:1. 20:4. 87)

Objective To determine the optimum harvest time of Scutellaria barbata D.Don with flavonoids contents as the index. Methods The content of total flavonoids in Scutellaria barbata D. Don was determined by ultraviolet-visible spectrophotometry with scutellarin as standard control, and scutellarin and cosmosiin in Scutellaria barbata D. Don were determined by HPLC. Results The contents of total flavonoids and scutellarin in Scutellaria barbata D.Don of different harvest time were complied with the quality standards in the China Pharma Copoeia, and reached the peakin blossom . The contents of total flavonoids, scutellarin and cosmosiin were (45.74±0.95) ,(5.58±0.16 ) and (17.39±0.42) mg?g-1 , respectively. Conclusion The Scutellaria Barbata D.Don may be collected at the flowering time with luxuriant foliage.