Objective To investigate the changes in psychological state after occupational exposure in the AIDS occupational exposure population and related influencing factors,and to provide baseline data and a basis for related departments to conduct mental health prevention and intervention for personnel with occupational AIDS exposure.Methods AIDS risk assessment was performed for all personnel with occupational AIDS exposure in 2014 in Nanning,China,and the Symptom Checklist-90 (SCL-90) psychological scale was used for psychological state evaluation at 24 hours,1 week,and 3 months after occupational exposure in all persons who met the research criteria.Results Most of the persons with occupational AIDS exposure came from secondary and tertiary hospitals (85％),and nurses accounted for the highest percentage (78.3％).The age ranged from 21 to 50 years,and the mean age was 31.02+7.92 years.The persons with occupational AIDS exposure aged 20～29 years accounted for the highest percentage (51.6％),and most persons (76.7％) graduated from junior colleges.Compared with the adult norm,there was significant increases in the total psychological score and the number of positive items after occupational exposure (P＜0.05).The scores of all items at 24 hours were significantly higher than those at the other time points,and the scores of all items gradually decreased over time (F=227.24,267.57,and 287.46,P＜0.05).Compared with the adult norm,there were significant increases in the factor points at 24 hours and significant reductions in the factor points at 3 months (P＜0.05).Compared with those at 24 hours,the factor scores at 3 months decreased significantly (P＜0.05).Conclusion Occupational AIDS exposure affects the mental status of related personnel,and the mental status at 24 hours after exposure is poor.Related departments should provide corresponding psychological counseling for the occupational exposure population at different exposure times.

Objective To investigate the changes in psychological state after occupational exposure in the AIDS occupational exposure population and related influencing factors,and to provide baseline data and a basis for related departments to conduct mental health prevention and intervention for personnel with occupational AIDS exposure.Methods AIDS risk assessment was performed for all personnel with occupational AIDS exposure in 2014 in Nanning,China,and the Symptom Checklist-90 (SCL-90) psychological scale was used for psychological state evaluation at 24 hours,1 week,and 3 months after occupational exposure in all persons who met the research criteria.Results Most of the persons with occupational AIDS exposure came from secondary and tertiary hospitals (85％),and nurses accounted for the highest percentage (78.3％).The age ranged from 21 to 50 years,and the mean age was 31.02+7.92 years.The persons with occupational AIDS exposure aged 20～29 years accounted for the highest percentage (51.6％),and most persons (76.7％) graduated from junior colleges.Compared with the adult norm,there was significant increases in the total psychological score and the number of positive items after occupational exposure (P＜0.05).The scores of all items at 24 hours were significantly higher than those at the other time points,and the scores of all items gradually decreased over time (F=227.24,267.57,and 287.46,P＜0.05).Compared with the adult norm,there were significant increases in the factor points at 24 hours and significant reductions in the factor points at 3 months (P＜0.05).Compared with those at 24 hours,the factor scores at 3 months decreased significantly (P＜0.05).Conclusion Occupational AIDS exposure affects the mental status of related personnel,and the mental status at 24 hours after exposure is poor.Related departments should provide corresponding psychological counseling for the occupational exposure population at different exposure times.

OBJECTIVETo construct eukaryotic expression vectors for different domains (V and VC1) of the extracellular region of the receptor of advanced glycation end products (RAGE) and investigate the roles of these domains in prostate cancer.

METHODSThe coding sequence of V and VC1 domains was amplified from the plasmid pcDNA3-HA-RAGE by PCR and cloned into the pcDNA3-HA vector following routine procedures. After identification by PCR and sequencing, the vectors including V and VC1 domains were transfected into PC-3 cells. Western blotting and immunofluorescence were used to detect the expression and distribution of the expressed products in transfected PC-3 cells.

RESULTSThe expression vectors containing V and VC1 domains of RAGE were successfully constructed as confirmed by PCR and DNA sequence analysis. The V and VC1 domains of RAGE were highly expressed and showed a cytoplasmic distribution in transfected PC-3 cells.

CONCLUSIONThe constructed eukaryotic expression vectors for V and VC1 domains of RAGE can be efficiently expressed in prostate cancer cells.

Cell Line, Tumor ; Cloning, Molecular ; Genetic Vectors ; Humans ; Male ; Plasmids ; Prostatic Neoplasms ; genetics ; Receptor for Advanced Glycation End Products ; Receptors, Immunologic ; metabolism ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; Sequence Analysis, DNA ; Transfection