Objective To clone the genes related to pregnancy induced hypertension (PIH) for the study of PIH pathogenesis. Methods The differential expressed cDNA of PIH and normal pregnant placenta were cloned using modified polymerase chain reaction based subtractive hybridization. The differential cDNA were sequenced. Results Ammong 86 clones from subtractive hybridization, 14 clones containing differential expressed gene fragments in PIH and normal placenta were identified, and these might be the genes associated with PIH. Compared with the expressed sequence tags (EST) in dbEST of National Center for Biotechnology Information, it showed that 11 of them were known gene fragments, and three were unknown. The three unknown gene fragments were registered in GenBank, the accession number is AF 232216, AF 232217, AF 233648 respectively. Conclusions Fourteen PIH related genes were cloned with subtractive hybridization. A new approach to study on the pathogenesis of PIH is presented.

The counts of the chromosome and karyotypeanalysis of coix lachryma-jobi L. (a traditional Chi-nese medicinal herb) are represented in this pa-per. The results show that the number of the chro-mosome of coix lachryma-jobi L. is 2n=20. All ofthe homologous chromosomes have median kine-tochrores. The absolute length of the chromo-somes tanges from 2. 26～10. 5?m. The relativelength is 7. 19～13. 02%. The karyotype formulais K (2n)=20=20m. These results provide morecritical cytological criteria for identification of Coixlachryma--jobi L. on the chromosomal level. Inaddition, It can be provided the data for studyingof the fraditional Chinese medicinal herbs againstcancer.

Animals ; Cdc20 Proteins/metabolism* ; Cell Line ; Embryo, Mammalian/embryology* ; Embryonic Development ; Female ; Infertility, Female/metabolism* ; Mice ; Mutation ; Oocytes/metabolism*