1.Multi-component quantitative method of Chinese herbal additives based on fingerprint.
Juanmin LUO ; Jibao CAI ; Xue XIAO ; Chan FU ; Kun LIAO ; Yiming WANG ; Guoan LUO
China Journal of Chinese Materia Medica 2011;36(10):1295-1297
OBJECTIVETo establish a full-quantified fingerprint method for analyzing Chinese herbal additives by HPLC.
METHODA HPLC in combination with PDA detector was applied with a phenomenex luna C18 (4.6 mm x 250 mm, 5 microm) column by gradient elution using acetonitrile-0.1% formic acid solution as the mobile phase. The flow rate was 1 mL x min(-1) and the detection wavelength was set at full spectrum scan.
RESULTAccording to the selected chromatographic conditions, the full-quantified fingerprint of the Chinese herbal additive has good precision, reproducibility, stability and recovery.
CONCLUSIONThe HPLC method developed for simultaneous determination of seven compounds is simple and valid. It can be used for quality evaluation and quality control of Chinese herbal additive and its processing products.
Chemistry, Pharmaceutical ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis
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