Objective To study the effects of different concentration of interleukin(IL)-17 on the expression of receptor activator of nuclear factor-κB ligand (RANKL) and osteoprogeterin (OPG) in human periodontal ligament fibroblasts (HPDLF) and explore the relationship between IL-17 and orthodontic root resorption.Methods HPDLF cell line was established through the tissue pieces culture method in vitro.HPDLF were stimulated by IL-17 with five different concentrations(0,5,10,20,40 ng/mL) for 24 h.The expression level of mRNA and protein of RANKL and OPG in HPDLF were detected by RT-PCR and ELISA,respectively.Results HPDLF expressed RANKL and OPG in 0 ng/mL group.The expression amount of mRNA and protein of RANKL in HPDLF was positive correlation with the concentration of IL-17 in 0 to 20 ng/mL group.The expression amount of mRNA and protein of OPG was negative correlation with the concentration of IL-17 in 5 to 20 ng/mL group.The ralative RANKL/OPG ratio of mRNA and protein were positive correlation with the concentration of IL-17 in 0 to 20 ng/mL group.Conclusion HPDLF expresses RANKL and OPG in the absence of IL-17.IL-17 enhances the expression of RANKL and inhibits the expression of OPG in HPDLF.

Objective To explore the effects of mobile APP on health education in inpatients with stroke. Methods From August to December, a total of 260 stroke patients in the Neurology Department of Xuanwu Hospital Capital Medcical University were selected by convenience sampling method. All the patients were asked to install the mobile APP and given health education using the health education module. The mastery degree of stroke related knowledge and nurse satisfaction were investigated before the installation of the APP and before discharge. Results The overall hits of health education mode was 1 989 times. After using APP intervention, the awareness of stroke patients was higher than that before intervention, the difference was statistically significant (P<0.001). The nurse satisfaction of health education module in the APP was 100%. Conclusions The effects of the mobile APP on stroke patients' health education are optimal, which could be further promoted in clinical practice, providing a foundation for effective stroke secondary prevention.