AIM: To explore the immunity modulation function of aqueous of Forsythia suspense (AFS) and its possible mechanisms. METHODS: Rats of burned model group were burned with vapor under 3mpa pressure and 108℃ temperature for 8 seconds to achieve deep partialthickness bum, to make a thirty percent total body surface area (TBSA)bum. The experiment were divided into five groups: Control group: without any treatment; 8 PBH group: 8 h after burn; the rats of AFS1 guoup, AFS2 group and AFS3 group of them were given AFS 5 g/kg, 2.5 g/kg, 1.25 g/kg once a day by Po. pathway for seven days before burns, respectively. Rats were sacrificed before and 8h after burn, The percentage of Treg cells in CD4~+ T cells was detected by flow cytometry(FCM) ; the expression of Foxp3 mRNA on splenocytes were measured by RT-PCR, and the protein of Foxp3 activity was evaluated by immunohistochemistry staining. RESULTS: Compared with Control group, the expression of Foxp3mRNA and protein on the splenocytes were upregulated markedly(P <0.01), and the percentage of Treg were significantly increased (P < 0.01) in the 8PBH group. AFS1, AFS2 and AFS3 significantly attenuated these increases (P < 0.01), which was dose-dependent. CONCLUSION: AFS has immunity modulation function and mechanism of it is corrected with Foxp3 mRNA on splenocytes.

OBJECTIVE To evaluate the feasibility and sterile effect of low-temperature plasma sterilizer produced by Chinese Academy of Sciences for the sterilization of arthroscope surgical instruments.METHODS To explore the cleaning,package,arrangement and sterile effect monitoring of instruments in the process of arthroscope sterilization using low-temperature plasma sterilizer.RESULTS In the sterilization process of arthroscope surgical instruments using low-temperature plasma sterilizer,the chemical indicators and indicating patch were discolored,the monitoring of bio-box and the sterilization monitoring of surgical instruments were all negative.CONCLUSIONS Using the low-temperature plasma sterilizer machine for the sterilization of endoscope surgical instruments can ensure the security of sterilization,make less injury of precise instruments,shorten the sterilization time,accelerate the surgical turnover,enhance the frequency of surgical instruments application,and increase the efficiency.

Objective:To investigate the risk factors for postpartum glycometabolism in patients with gestational diabetes mellitus (GDM), providing a new idea for clinical prevention of type 2 diabetes mellitus.Methods:Pregnant women who underwent regular prenatal examination in Cixi People's Hospital, China between February 2019 and January 2020 were included in this prospective cohort study. The clinical data before and during pregnancy were collected. These pregnant women were followed up for 6 weeks and 6 months postpartum. Oral glucose tolerance test (OGTT) with 75 g of glucose was performed. The clinical end point was abnormal glucose metabolism. Kaplan-Meier (KM) univariate analysis and multivariate Cox regression analyses were used to analyze the risk factors for abnormal glucose metabolism postpartum in patients with GDM.Results:A total of 252 eligible patients were included in this study. Among them, 212 patients finished 6-week follow-up, and 149 patients finished 6-month follow-up. Among the 212 patients who finished 6-week follow-up postpartum, 87 (41.04%) patients had abnormal glucose metabolism, 3 (1.42%) patients had impaired fasting glucose, 66 (31.13%) had impaired glucose tolerance, and 18 (8.49%) patients had type 2 diabetes mellitus. Among the 149 patients who finished 6-month follow-up postpartum, there were 2 new patients with impaired fasting glucose, 11 new patients with impaired glucose tolerance, and 3 new patients with type 2 diabetes mellitus. Overweight before pregnancy [body mass index (BMI) ≥ 24 kg/m 2] and1-hour OGTT (≥ 10.1 mmol/L) were the independent risk factors for abnormal glucose metabolism postpartum in pregnant patients with GDM ( OR = 2.273, 2.462; P = 0.039, 0.023; 95% CI = 1.495-3.051, 1.684-3.240). The Matsuda indexes and 60-minute insulinogenic index in the normal glucose metabolism group were significantly higher than in the abnormal glucose metabolism group ( t = 7.184 and 2.011, both P < 0.05). Conclusion:The incidence of abnormal glucose metabolism in patients with GDM at 6 months postpartum remains high. Overweight before pregnancy [body mass index ≥ 24 kg/m 2] and 1-hour OGTT (≥ 10.1 mmol/L) are the independent risk factors for abnormal glucose metabolism postpartum in pregnant patients with GDM.

Objective To construct the core competency evaluation scale for nurses in Department of Cardiology, and evaluate its psychometric characteristics. Methods The core competency evaluation scale for nurses in Department of Cardiology was developed by literatures review and clinical situation. A total of 176 nurses in 5 class Ⅲ grade A hospitals in Shaanxi Province were selected by convenient sampling method and investigated anonymously. Results The choice rates of all alternatives were lower than 80%. One item was deleted because its coefficient of variation was less than 20%. Discrimination correlation coefficients were between 0.340 - 0.732. The content validity index was 0.928. Five factors were extracted by exploratory factor analysis, with the cumulative contribution of 67.6%. Five factors were expected. The factor analysis confirmatory showed that χ2/df=4.013, SRMR=0.051, RMSEA=0.062. The split half reliability was 0.842; Cronbach's alpha was 0.856, and the retest reliability was 0.908. Conclusions The core competency evaluation scale for nurses in department of cardiology has good validity and reliability which can be used to evaluate the core competency.

Objective To investigate the effectiveness and safety of intravenous thrombolytic therapy with low-dose alteplase for elderly patients with acute ischemic stroke.Methods The elderly patients with acute ischemic stroke were enrolled prospectively (onset within 4.5 h,aged ≥75 years).They were randomly divided into either a low-dose group (0.6 mg/kg) or a standard-dose group (0.9 mg/kg).The primary outcome was the modified Rankin Scale (mRS) score determined the good outcome (mRS score 0-2) and poor outcome (mRS score ＞2) at 90 d after treatment;the secondary outcome was intracranial hemorrhage and symptomatic intracranial hemorrhage at 24 h after thrombolysis,and death within 90 d.Results A total of 65 elderly patients with acute ischemic stroke were enrolled,including 32 in the low-dose group and 33 in the standard-dose group.There were no significant differences in all baseline clinical data between the two groups.Compared with before treatment,the proportions of patients with the mRS score 0-2 were significantly higher in both groups at 7 d,14 d,and 90 d after treatment,but there were no significant differences in good outcome rate at the same time point after treatment between the two groups.In addition,there were no significant differences in intracranial hemorrhage (36.4％ vs.25.0％;x2 =0.985,P =0.321),symptomatic intracerebral hemorrhage (21.2％ vs.9.4％;x2 =1.749,P =0.186) at 24 h after treatment,and incidence of death within 90 d after treatment (12.1％ vs.6.3％;x2 =0.151,P=0.697)between the two groups.Conclusions The effectiveness and safety of intravenous thrombolytic therapy with low-dose alteplase (0.6 mg/kg) in elderly patients with acute ischemic stroke is equivalent to the standard dose.

Objective:The study aimed to investigate the effect of exosomes derived from bone marrow mesenchymal stem cells (BMSC-Exo) in promoting angiogenesis of fat grafts by regulating Notch signaling pathway.Methods:Fat from the abdomen of a 30-year-old healthy female who received liposuction in the Department of Plastic Surgery of the First Affiliated Hospital of Air Force Military Medical University in August 2020 was collected. Fat transplantation models were established using 12 BALB/c nude mice, with 0.5 ml of processed fat particles injected into the left and right sides of the backs. 100 μl BMSC-Exo (50 μg) was injected into the left side of the back of nude mice once per week for 4 weeks after fat grafting, which was the exosome group. The right side of the back was injected with 100 μl of phosphate-buffered saline at the same time points for 4 weeks after fat grafting, which was the control group. The following items were observed: (1) Fat grafts were collected and weighed at 4 weeks and 8 weeks after transplantation, the retention rate was calculated. (2) HE staining and immunofluorescence staining of Perilipin A was used to visualize the structure and integrity of adipocytes at 8 weeks after transplantation. (3) At 8 weeks after transplantation, immunohistochemical staining was used to detect CD31 positive cells, calculate micro vessel density, detect Notch1 and Notch4 positive cells and calculate the proportion of positive area.(4) At 8 weeks after transplantation, the relative expressions levels of Notch1, Notch4 and VEGF mRNA in fat grafts were detected by real-time fluorescence quantitative PCR. The data were analyzed by Graphpad Prism 8.0. T-test was used for comparison between groups. P<0.05 indicated the difference was statistically significant. Results:(1) At 4 and 8 weeks after transplantation, the retention rates of adipose tissue wet weight in the control group were (41.15±9.68)% and (34.30±6.45)% respectively. The results in the exosome group were (61.77±10.98)% and (55.93±5.89)%, which were higher than those in the control group ( P<0.05). (2) At 8 weeks after transplantation, the structural integrity of adipocytes in the exosome group was better than the control group, with more neovascularization. There were more intact Perilipin A positive adipocytes in the exosome group and less in the control group. (3) At 8 weeks after transplantation, the density of micro vessels in the control group was 4.67±0.57, which was significantly lower than that in the exosome group (13.00±2.00, P<0.05). The proportion of Notch1 and Notch4 positive area per visual field in the control group was lower than that in the exosome group [(0.53±0.28)% vs. (1.67±0.11)%, (0.11±0.06)% vs. (1.20±0.24)%, P<0.05]. (4) The mRNA expression levels of Notch1, Notch4 and VEGF were 1.53±0.20, 1.50±0.26 and 2.56±0.55 in the exosome group, which were significantly higher than those in the control group (1.00+ 0.00) ( P<0.05). Conclusions:BMSC-Exo may promote angiogenesis and improve retention rate of transplanted fat grafts through the elevation of VEGF expression and the activation of Notch signaling pathway.

Objective:The study aimed to investigate the effect of exosomes derived from bone marrow mesenchymal stem cells (BMSC-Exo) in promoting angiogenesis of fat grafts by regulating Notch signaling pathway.Methods:Fat from the abdomen of a 30-year-old healthy female who received liposuction in the Department of Plastic Surgery of the First Affiliated Hospital of Air Force Military Medical University in August 2020 was collected. Fat transplantation models were established using 12 BALB/c nude mice, with 0.5 ml of processed fat particles injected into the left and right sides of the backs. 100 μl BMSC-Exo (50 μg) was injected into the left side of the back of nude mice once per week for 4 weeks after fat grafting, which was the exosome group. The right side of the back was injected with 100 μl of phosphate-buffered saline at the same time points for 4 weeks after fat grafting, which was the control group. The following items were observed: (1) Fat grafts were collected and weighed at 4 weeks and 8 weeks after transplantation, the retention rate was calculated. (2) HE staining and immunofluorescence staining of Perilipin A was used to visualize the structure and integrity of adipocytes at 8 weeks after transplantation. (3) At 8 weeks after transplantation, immunohistochemical staining was used to detect CD31 positive cells, calculate micro vessel density, detect Notch1 and Notch4 positive cells and calculate the proportion of positive area.(4) At 8 weeks after transplantation, the relative expressions levels of Notch1, Notch4 and VEGF mRNA in fat grafts were detected by real-time fluorescence quantitative PCR. The data were analyzed by Graphpad Prism 8.0. T-test was used for comparison between groups. P<0.05 indicated the difference was statistically significant. Results:(1) At 4 and 8 weeks after transplantation, the retention rates of adipose tissue wet weight in the control group were (41.15±9.68)% and (34.30±6.45)% respectively. The results in the exosome group were (61.77±10.98)% and (55.93±5.89)%, which were higher than those in the control group ( P<0.05). (2) At 8 weeks after transplantation, the structural integrity of adipocytes in the exosome group was better than the control group, with more neovascularization. There were more intact Perilipin A positive adipocytes in the exosome group and less in the control group. (3) At 8 weeks after transplantation, the density of micro vessels in the control group was 4.67±0.57, which was significantly lower than that in the exosome group (13.00±2.00, P<0.05). The proportion of Notch1 and Notch4 positive area per visual field in the control group was lower than that in the exosome group [(0.53±0.28)% vs. (1.67±0.11)%, (0.11±0.06)% vs. (1.20±0.24)%, P<0.05]. (4) The mRNA expression levels of Notch1, Notch4 and VEGF were 1.53±0.20, 1.50±0.26 and 2.56±0.55 in the exosome group, which were significantly higher than those in the control group (1.00+ 0.00) ( P<0.05). Conclusions:BMSC-Exo may promote angiogenesis and improve retention rate of transplanted fat grafts through the elevation of VEGF expression and the activation of Notch signaling pathway.

Objective:To explore the effects of perianesthesia position nursing model based on enhanced recovery after surgery (ERAS) in patients undergoing thoracoscopic surgery.Methods:Totally 100 patients undergoing thoracoscopic pulmonary surgery in Huzhou Central Hospital between July and December 2019 were selected by convenient sampling and divided into the control group and the experimental group according to the random number table, with 50 cases in each group. Patients in the experimental group received perianesthesia position nursing, while patients in the control group received conventional position nursing. The time of position placement, number of placement personnel, intubation time, comfort and pain were compared between the two groups.Results:There were statistically significant differences in the placement time and number of placement personnel between the two groups ( P<0.05) . The GCQ score of the experimental group was higher, while the VAS score was lower than that of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The perianesthesia position nursing model based on ERAS can shorten the time of posture placement, reduce the pain, and improve the comfort in patients undergoing thoracoscopic surgery.

Objective:To reveal the effect of β-sitosterol on cerebral ischemia-reperfusion injury (CIRI) in rats and whether its mechanism is related to endoplasmic reticulum stress (ERS).Methods:Fifty-three CIRI rats (CIRI models established by modified Longa method) were randomly divided into model group ( n=14), β-sitosterol low-dose group ( n=13), β-sitosterol medium-dose group ( n=13) and β-sitosterol high-dose group ( n=13); 12 rats underwent the same operation without blocking the middle cerebral artery were selected as sham-operated group. Rats in the sham-operated group and model group were given intragastric administration of 1 mL 5 g/L sodium carboxymethyl cellulose daily. Rats in the β-sitosterol low-dose group, β-sitosterol medium-dose group and β-sitosterol high-dose group were given intragastric administration of 1 mL β-sitosterol at 10, 20 and 40 mg/kg/d (dissolved in 5 g/L sodium carboxymethyl cellulose), respectively, for 14 consecutive d. Neurological function was evaluated according to Zea Longa 5 method. Rats were sacrificed and brain tissues were collected. Volume of cerebral infarction was measured by 2,3,5-triphenyl tetrazolium chloride (TTC) staining. Brain injury and neuronal apoptosis were evaluated by HE staining, Nissl staining and TUNEL. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) contents were detected by water-soluble tetrazolium 1 (WST-1) method, colorimetric method or thiobarbituric acid (TBA) method, respectively. The mRNA and protein expression levels of protein kinase R-like endoplasmic reticulum kinase (PERK), inositol-requiring enzyme-1 (IRE-1), activated transcription factor-6 (ATF-6), glucose-regulated protein 78 (GRP78), C/EBP homologous protein (CHOP) and Caspase-12 in the brain tissues were detected by qRT-PCR or Western blotting. Results:Compared with the sham-operated group, the model group had significantly increased neurological function score, cerebral infarction volume and TUNEL positive rate, decreased SOD and GSH-Px content, increased MDA content, and increased mRNA and protein expressions of PERK, IRE-1, ATF-6, GRP78, CHOP and Caspase-12 ( P<0.05). Compared with the model group, the β-sitosterol low-dose group, β-sitosterol medium-dose group and β-sitosterol high-dose group had significantly decreased neurological function score, cerebral infarction volume, and TUNEL positive rate, increased SOD and GSH-Px content, and decreased MDA content ( P<0.05); the β-sitosterol low-dose group, β-sitosterol medium-dose group and β-sitosterol high-dose group had significantly decreased mRNA and protein PERK expressions (mRNA: 2.17±0.17, 1.79±0.07 and 1.33±0.07; protein: 5.11±0.52, 2.91±0.26 and 1.98±0.17), IRE-1 expressions (mRNA: 1.75±0.18, 1.65±0.08 and 1.32±0.08; protein: 5.00±0.31, 4.05±0.27 and 1.98±0.14), ATF-6 expressions (mRNA: 2.24±0.12, 1.77±0.14 and 1.37±0.13; protein: 4.93±0.45, 4.04±0.30 and 3.10±0.20), GRP78 expressions (mRNA: 2.67±0.16, 2.11±0.16 and 1.69±0.11; protein: 5.02±0.38, 2.97±0.26 and 2.05±0.22), CHOP expressions (mRNA: 2.01±0.16, 1.70±0.19 and 1.40±0.10; protein: 4.92±0.39, 4.02±0.27 and 3.08±0.22) and Caspase-12 expressions (mRNA: 1.85±0.09, 1.61±0.09 and 1.30±0.09; protein: 3.03±0.20, 2.19±0.11 and 1.82±0.11) compared with the model group (mRNA: 2.99±0.28, 2.27±0.12, 2.57±0.21, 3.46±0.20, 2.50±0.23 and 2.35±0.16; protein: 6.98±0.48, 6.03±0.58, 5.98±0.63, 7.10±0.45, 6.00±0.53 and 5.02±0.43, P<0.05). Conclusion:β-sitosterol attenuates CIRI in rats, whose mechanism may be related to inhibition of ERS signal pathway.

To study the genic and non-genic regulation of 50 Hz 0.6 mT pulsed electromagnenic fields (PEMF) on rat calvarial osteoblasts (ROB) differentiation.ROBs were achieved by enzyme digestion, and treated with 50 Hz 0.6 mT PEMFs for 1.5 hours after subculture. The alkaline phosphatase (ALP) activity, mRNA transcription of ALP, Runx2 and OSX and protein expression of Runx2 and OSX were detected at 0, 3, 6, 9 and 12 hours after PEMF treatment.The ALP activity at 3 hours after treatment was significantly higher than that in the control(<0.01), while the mRNA transcription of ALP began to increase at 6 hours after treatment. The mRNA transcription of Runx2 increased immediately after treatment and regressed at 6 hours, then increased again. The protein expression of it corresponded but with a little lag. The mRNA transcription of OSX also raised instantly after treatment, then returned to the level of control at 6 hours, and lower than control at 12 hours significantly. The protein expression of it also corresponded but with a bit delay.There are genic regulation for the protein expression of Runx2 and OSX, and non-genic regulation for the ALP activity on the process of 50 Hz 0.6 mT PEMFs prompts ROBs differentiation.
Alkaline Phosphatase ; metabolism ; radiation effects ; Animals ; Cell Differentiation ; genetics ; radiation effects ; Cells, Cultured ; Core Binding Factor Alpha 1 Subunit ; metabolism ; radiation effects ; Electromagnetic Fields ; Osteoblasts ; chemistry ; radiation effects ; Osteogenesis ; genetics ; radiation effects ; Rats ; Transcription Factors ; metabolism ; radiation effects