Objective To clone and express Chlamydia trachomatis (Ct) heat shock protein 60 (hsp60) gene. Methods The hsp60 gene fragment was amplified from Ct chromosomal DNA by PCR. After purification and digestion with enzymes Sal I and Not I , the hsp60 gene fragment was inserted into the compatible site of prokaryotic expression vector pET-28a. The constructed recombinant plasmid was identified by PCR, restriction enzyme cleavage and sequencing, then, it was transfected into an expression strain Escherichia coli BL21 (DE3). The expression of fusion protein was induced by isopropy-β-D- thiogalactoside (IPTG) in the host bacteria, and the expressed product was identified by sodium dodecyl sulfate polyacrylamide gel electropheresis (SDS-PAGE) and Western-blot. Results PCR and restriction enzymes cleavage analysis confirmed that the hsp60 gene was successfully cloned into the recombinant plasmid. DNA sequencing showed that the sequence of cloned gene was fully consistent with the published sequence in Genebank. As revealed by SDS-PAGE, the size of expressed fusion protein approximated 60 kilodaltons, and Western-blot confirmed the expressed product to be the expected protein. The final concentration of fusion protein was 17.85 mg/L with a purity of more than 90%. Conclusions A recombinant expression plasmid pET-28a-hsp60 is successfully constructed in this study, and soluble hsp60 protein is expressed by the recombinant plasmid-transfected E. coli.

Objective:To explore the application effect of continuous nursing with Wechat as medium in patients with chronic heart failure.Methods:Using the convenient sampling method, a total of 102 patients with chronic heart failure who were treated in Zhejiang Hospital were selected as the research objects from June 2019 to June 2020. According to random number table method, they were divided into the observation group ( n=52) and the control group ( n=50) . The control group was given routine nursing intervention, while the observation group was given continuous nursing intervention with Wechat as medium. The effect of intervention was evaluated by Self-rating Anxiety Scale (SAS) , Self-rating Depression Scale (SDS) , Exercise of Self-Care Agency Scale (ESCA) and 36-item Short-Form (SF-36) . Results:There were no statistically significant differences in the scores of SAS, SDS, dimensions of ESCA and dimensions of SF-36 between the two groups at admission ( P>0.05) . Three months after intervention, SAS and SDS scores of patients in the observation group were lower than those in the control group, and scores of dimensions of ESCA and SF-36 were higher than those in the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The continuous nursing with Wechat as medium has good application effect in patients with chronic heart failure, which can reduce the psychological mood of patients, improve the self-care ability and improve quality of life.