目的观察脉冲电磁场治疗骨质疏松症的疗效。方法对60例骨质疏松症患者应用XT-2000B型骨质疏松治疗仪进行复合脉冲电磁场(C-PEMFs)治疗。结果治疗30次后,总有效率96.7%。结论C-PEMFs是一种安全、有效的治疗骨质疏松症的方法。

It's observed that periarthritis of shoulder is treated with early kinesitherapy intervention combining with diathcrmy. The clinic observation with curative effect the severity of periarticular tenderness prewith posttreatment and the mobility of the shoulder pre-with posttreatment between the treatment and control groups are different. The statistical values are X2=12.55,P<0.005,X2=14.10,P<0.005,X2=14.51,P<0. 005 respectively. The method can shorten the course of treatment.

Objective To investigate the effect of hyperlipidemia (HL) on the pancreatic injuries during acute necrotizing pancreatitis (ANP) as well as the activation of nuclear factor kappa B ( NF-κB) and pancreatic acinar cell apoptosis. Methods Fifty SD male rats were randomly divided into control group, hyperlipidemia ( HL) group, ANP group and HL + ANP group. Rats in control group were fed with balanced diet for 2 weeks, and underwent opening and closing of the abdomen. Rats in HL group received fat emulsion lavage, and then underwent opening and closing of the abdomen. Rats in ANP group were fed with balanced diet for 2 weeks, and were induced by retrograde injection of sodium taurocholate into the bili-pancreatic duct to establish the ANP model. Rats in HL + ANP group received fat emulsion lavage for 2 weeks, and were induced by retrograde injection of sodium taurocholate into the bili-pancreatic duct to establish the ANP model. NF-κB p65 and Fas, FasL protein expression were determined by immunohistochemical method and the apoptosis in pancreatic acinar cell were detected by TUNEL. Results Two weeks after fat emulsion lavage, rats in HL and HL + ANP group had significantly higher serum level of lipid. Lipid vacuoles were present in some pancreatic cells in the rats of HL group, and middle number of inflammatory cells infiltration was found, the activation of NF-κB was slightly enhanced; and the expression of Fas, FasL was also enhanced. Apoptosis index increased from (0.62 ±0.28)% to (3.3S ±1.12)%. Massive pancreatic tissue necrosis, large amount of inflammatory cells infiltration was found in ANP group. NF-κB p65 was present in large number of pancreatic nucleus. The expression of Fas and FasL was also increased; the apoptosis index was (2.20±1.78)%. The degree of pancreatic necrosis was greater in HL + ANP group than that in ANP group (3.4 ± 0.7 vs. 2.4 ± 1.1, P <0.05). The positive rate and density of NF-κB expression was higher than that in ANP group; and the expression of Fas and FasL was decreased more than that in ANP group; the apoptosis index was (0.93 ± 0.87)% , which was significantly lower than that in ANP group (P <0.05). Conclusions High level of lipid can increase the activation of NF-κB and inhibit the acinar cell apoptosis, and decrease the expression of Fas and FasL, increase the pancreatic tissue necrosis, therefore in order to attenuate the injury of pancreas, it is essential to control blood lipid.

Objective To investigate the changes and clinical significance of the platelet parameters in tumor patients before or after radiochemotherapy.Methods The platelet parameters(PLT,PCT,MPV,PDW)were detected in 65 cases of tumor patients before and after radiochemotherapy by the Sysmex XN-1000 automatic hemacytology analyzer.Then the detection results were com-pared with those in the control group.Results Compared with the control group,PLT and MPV before therapy in the tumor pa-tients were significantly increased(P <0.01),but PCT and PDW had no statistical differences between the two goups(P >0.05). PLT,PCT and MPV after radiochemotherapy in the tumor patients were significantly lower than those before treated(P <0.01 ), but no statistically significant difference in PDW was found(P >0.05).Conclusion The measuration of platelet parameters in the tumor patients with radiochemotherapy has certain clinical significance to observe the status of the bone marrow suppression and es-timate the state of illness.

Objective: To analyze plasma levels of catestatin (CST) in patients with different blood pressure (BP) and metabolic status.
Methods: Our research included in 3 groups: Control group,n=60 subjects with normal BP from physical examination in our hospital; 131 patients with essential hypertension (EH) were divided into 2 groups: Isolated EH group,n=90 and MS (metabolic syndrome) group,n=41. Plasma levels of CST, norepinephrine (NE) were examined, the ratio of CST/NE was calculated, and those indexes were compared among different groups.
Results: Compared with Control group, plasma levels of CST and the ratios of CST/NE were decreased in Isolated EH and MS groups,P<0.05-0.01. As BP increasing, plasma levels of CST were gradually decreased; compared with Isolated EH group, plasma level of CST and CST/NE were reduced in MS group,P<0.05.
Conclusion: Plasma levels of CST and the ratio of CST/NE were obviously decreased in EH patients, CST was even lower in MS patients which implied that CST might be involved in the development of EH and MS.

OBJECTIVE:To investigate the effects of Bushen huoxue cupailuan decoction on clinical effects and related index-es of infertility induced by polycystic ovary syndrome. METHODS:A total of 120 cases of polycystic ovary syndrome of kidney de-ficiency and blood stasis selected from our hospital were divided into observation group and control group according to random num-ber table,with 60 cases in each group. From the 5th day of menstruation,control group was given Clomiphene citrate capsules 50 mg,qd;observation group was given 200 mL of Bushen huoxue cupailuan decoction,morning and night. Atreatment course lasted for 3 menstrnal cycle,both groups were treated for 1-2 courses. The number of follicles,endometrial thickness,ovarian volume and serum hormone levels (INS,LH,FSH,PRL,T,E2) were observed in 2 groups before and after treatment. The pregnancy rate were compared between 2 groups after treatment. RESULTS:Before treatment,there was no statistical significance in the num-ber of follicles,endometrial thickness,ovarian volume or serum hormone levels between 2 groups(P>0.05). After treatment,the number of follicles,endometrial thickness and ovarian volume in 2 groups were all increased;the improvement of observation group was better than control group,with statistical significance(P<0.05). After treatment,the levels of FSH and E2 in 2 groups were increased significantly;the level of serum hormone in observation group was significantly better than control group,with sta-tistical significance(P<0.05). The pregnancy rate of observation group was 63.33%,which was significantly higher than 46.67%of control group,with statistical significance(P<0.05). CONCLUSIONS:Bushen huoxue cupailuan decoction can effectively im-prove serum hormone lever,ovary condition and pregnancy rate in the infertility patients induced by polycystic ovary syndrome.

Administration, Topical ; Humans ; Immunosuppressive Agents ; administration & dosage ; adverse effects ; therapeutic use ; Lichen Planus, Oral ; complications ; drug therapy ; pathology ; Oral Ulcer ; drug therapy ; etiology ; Tacrolimus ; administration & dosage ; adverse effects ; therapeutic use ; Treatment Outcome

Objective To screening up-regulated protein kinases and their inhibitors in order to provide new targets for molecular thera-py of nasopharyngeal cancer.Methods GEO database and SAM software were employed to screen the up-regulated protein kinase gene in nasopharyngeal cancer.Based on DAVID database,the regulating functions of kinases were identified.The inhibitors of up-regulated kinase genes were identified by Selleckchem database.Literature mining was used to screen the potential anti-cancer drugs.Results Totally 2360 differentially expressed genes including 21 up-regulated protein kinases (CHEK1,CHEK2,PRKDC,AURKA,VRK2,STK17A,MELK,NU-AK1,TRPM7,MASTL,AXL,BUB1,BUB1B,CDK4,TTK,CDC7,CASK,AKT3,TBK1 and PBK)were identified in the whole genome profi-ling (Fold Change≥2,P <0.05).The results of function analysis showed the up-regulated genes were enriched in 10 function terms such as‘protein amino acid phosphorylation’‘phosphorylation’‘phosphate metabolic process’‘mitotic cell cycle’‘cell cycle phase’‘regulation of cell cycle’,and so on.The Selleckchem database analysis showed there were 9 up-regulated protein kinases equipped with 51 inhibitors which were proved already.The results of literature mining showed that 18 inhibitors of them had a few studies (less than 10 literatures)in cancer terms,and there was a potential to become new drugs to treat nasopharyngeal cancer.Conclusion A total of 21 up-regulated protein kinases were identified,and they might promote the nasopharyngeal carcinoma by regulating functions such as the cell-cycle control pathway.Their ki-nase inhibitors may have a potential role in anti-cancer treatment,which provided a new target point for molecular therapy of nasopharyngeal cancer.

Objective To explore the possibility of human neutrophil peptide 1 (HNP1) gene engineering, we construct the eukaryotic expression vector carrying HNP1 gene. Methods With RNA extracted from human neutrophil cell as template, cDNA encoding mature HNP1 was amplified by RT-PCR, and then it was inserted into vector pMD18-T. After restriction endonuclease digestion and DNA sequencing confirmation the gene was subcloned into eukaryotic expression plasmid pcDNA3.1/V5-His-TOPO to construct a recombinant expression plasmid pcDNA3.1/V5-His-TOPO/HNP1, then the recombinant plasmid was transfected into COS-7 cells by lipofectamine, and the expressed product was identified by biotin-avidin enzyme-linked immunosorbent assay ( BA-ELISA). Results The sequence of HNP1 completely matched those published in GenBank, thus eukaryotic expression vector pcDNA3.1/V5-His-TOPO/HNP1 was constructed correctly. The ELISA results showed that the eukaryotic expression plasmid pcDNA3.1/V5-His-TOPO/HNP1 could temporarily express HNP1 in COS-7 cells. Conclusion The successful construction and expression of pcDNA3.1/V5-His-TOPO/HNP1 pave the way for the stable expression HNP1 in mammalian engineering cells.

Objective To compare the effort intensities in comprehensive quality development of military medical undergraduates of different sorts and to discuss its improvement.Methods Comprehensive quality of undergraduates of 2010 grade (630 persons including 16 cadres) was assessed by comprehensive quality assessment index system.Based on data envelopment analysis,ratio of actual output (comprehensive quality development) against ideal value was taken as progress effectiveness to reflect the comprehensive development.SPSS 16.0 software was used for statistical processing and analysis.Score of each quality was analyzed and nondimensionalize was performed.Undergraduates' progress effectiveness was compared using analysis of variance.Results In September 2011,progress effectiveness of undergraduates without military status was higher than that of soldiers in comprehensive quality,ideology and morality,science,culture,profession,psychological quality and basic ability (F =34.659,49.234,119.273,12.641,103.814,65.105,P ＜ 0.001).Progress effectiveness in the former was higher than that of the latter in physical fitness.(F =7.363,P ＜ 0.01).In March 2012,progress effectiveness of undergraduates without military status was higher than that of soldiers in comprehensive quality,ideology and morality,science,culture,profession,psychological quality,basic ability and physical fitness (F =28.017,10.847,33.559,24.630,48.767,10.460,38.251,P ＜0.01).Conclusions Comprehensive quality development of undergraduates is a continuous dynamic course.One year's observation shows that at early stage,all qualities of undergraduates without military status improve quickly except physical fitness with more subjective efforts.The two sorts of undergraduates belong to forward effect.