Objective To explore pediatric EEG in localized region abnormity and influence factors. Methods 51 children with EEG abnormity were retrospectively analyzed. Results In this group,there are 45 children with EEG abnormity in normal situation,and 6 of 51 childrened appear abnormity in induced tests. All the 51 childrened appeal abnormai brain wave in occipital region in varying degree. Conclusion Pediatric occipital region localization abnormal EEG may be hints related to children paroxysmal disorder, Miscellaneous factors can influence children paroxysmal disorder include all kinds of etiology, pathologic physiology signs and symptoms, age and individual variation ect.

Objective To observe the clinical therapeutic effect of Bushen Huoxue Zhuyun Decoction and its influence on the endometrial growth and the follicle, and supply scientific evidence for the clinical use of Bushen Huoxue Zhuyun Decoction. Methods 90 cases of patients with anovulatory sterility of kidney deficiency syndrome were divided into two groups randomly. Treatment group (50 cases) and control group (40 cases) were given Bushen Huoxue Zhuyun Decoction and clomiphene citrate respectively. Results In the treatment group, the cured rate was 24%, markedly effective rate was 56%, effective rate was 16%, and the total effective rate was 96%. In the control group, the cured rate was 15%, markedly effective rate was 17.5%, effective rate was 25%, and the total effective rate was 82.5%. There was significant difference between the two groups by statistical analysis (P 0.05). The thickness of uterine endometrium of pre-treatment and post-treatment in treatment group was significant by statistical analysis (P

Objective To discuss the value of EEG in early diagnosis and prognosis appraisal of children with the symptoms headed by"abdominal pain ferer"take the"abdominal pain,fever"as the first symptoms of viral encephalitis.Methods 150 cases of"abdominal pain,fever"as the first symptom diagnosed of viral encephalitis children were chosen for the EEG analysis before and after treatment.Results EEG abnormalities 117 cases,33 cases of normal.The EEG abnormality rate of abnormality is 78% ,showed diffuse or localized slow-wave number.There are two groups of children with temporal epileptiform discharges occur.There are 107 cases of abnormal EEG returned to normal and another 10 cases significantly improved in the 117 cases after symptomatic treatment of 1～2 weeks.Conclusion The EEG inspection is help with"abdominal pain,fever" as the first symptoms of viral encephalitis in children with early diagnosis,symptomatic treatment and prognosis.

Objective To detect the incidence and monality of the pediatric sepsis/severe sepsis,and to investigate the risk factors for poor prognosis in these patients.Methods From Jan.1 to Dec.31,2008,all pediatric patients admitted to PICU in Beijing Children's Hospital were prospectively surveyed.Both Chinese pediatric critical care scoring system and American guidelines for PICU admission and discharge were applied for screening subjects.The diagnosis criteria of pediatric sepsis/severe sepsis were made according to the definition determined on the International Pediatric Sepsis Consensus Conference held in the United States in early 2002.The qualified subjects were surveyed by questionnaire until discharge.After three months,the subjects were followed up by telephone.Results In a total of 742 children were screened.545 cases were critically ill,143 cases (26.2%) developed sepsis,and 104 cases (19.1%) deteriorated into a state af severe sepsis.Hospital mortality of severe sepsis was 21.2%,and that of sepsis was 0.7%.At the end of three months after discharged from hospital,mortality of severe sepsis was 30.8%.and that of sepsis was 1.4%.Patients under 3-year-old accounted for 72.5%.Pneumonia was the most common primary disease.The respiratory dysfunctions in severe sepsis cases were most common organ dysfunction (73.1%) . In surviving patients with severe sepsis,the PICU stay was significantly longer than that in deceased patients,and the PICU costs and average per capita costs were higher than the critically ill patients.Muhiple stepwise logistic regression analysis showed that pediatric critical illness score (PCIS score),capillary refilling Lime,respiratory dysfunction and hypoalbuminemia were the risk factors for death.Conclusions Sepsis/severe sepsis are common in the PICU,which have high mortality,high hospital charges.Infants and young children were the most commonly affected.Pneumoma was the most common primary disease.PCIS score.capillary filling time,respiratory dysfunction and hypoalbuminemia were the risk factors for death.

Atherosclerosis is a multifactorial disease and mediated by chronic inflammation from initiation to progression. Inflammatory factors of this process, such as C-reactive protein, interleukin-6, cell adhesion molecules and tumor necrosis factor-a, not only involve in atherosclerosis-related disease process, but also indicate the risk of cardiovascular events. Inflammatory factors level plays a significant role in diagnosis, treatment and prognosis of atherosclerosis-related disease, such as acute coronary syndrome. Recent studies have shown that there is a marked inflammatory response after stenting, which plays an important role in the vascular remodeling process, especially in-stent restenosis. Therefore, it is necessary to explore the molecular mechanism and pathological changes in the process of inflammation and to study prevention and treatment of the inflammation. We believe that inflammatory processes will be new targets of therapy in preventing or treating atherosclerosis and its complications.

Objective To investigate the effects of dexamethasone on the airway development and vasculogenesis in cultured human fetal lung exposed to hyperoxia.Methods Human fetal lung at the pseudoglandular stage of development were cultured either in normoxia(21%O2,5%CO2)or hyperoxia(95%O2,5% CO2)for 72 hours.Dexamethasone was added into the culture medium at the concentration of 10-6 mol/L.Whereas ethanol at the same concentration was as control.Harvested tissue wss stained for pancytokeratin to I dentify epithelial cells.CD31 aS a specific antibody of endothelial cell.The effects of lung morphometry were analyzed using computer-assisted image analysis software to ealeuhte the thickness of airways,surface area occupied by airways and the distance of vessels to the nearest airway as well as the surface area of blood vessels.Results The lung architecture remained largely unchanged in normoxia for 72 hours culture,whereas hyperoxic culture of lungs resulted in significant dilation of airways and thinning of epithelium.The surface area occopied by airways increased significantly(P＜0.05).The blood vessels in interstium were more close to the airway epithelium in the hyperoxic lung(50 μm)as compared with normoxia group.Meanwhile,the proportion of the blood wessels in interstium of hyperoxia group(4.0%)Was higher than those of normoxia group (4.2%)and hyperoxia with dexamethasone(5.4%)(P＜0.05).Dexamethasone has the protective effect on lungs exposed tO hyperoxia.Conclusion Our resulta showed that hyperoxia resulted in the simple airways and the decreased bkxxl vessel density in interstitium of cultared human fetal lung.Dexamethasone has protective effect on fetal lungs exposed to hyperoxia and can promote lung mature.

Radiotherapy is the main treatment for head and neck cancer,but it will result in adjacent tissue damage,including radioactive xerostomia,the most common complication.The mechanism of radiation-induced parotid damage injury is unclear.Parotid function test include detection of parotid salivary flow and salivary flow rate,parotid SPECT,CT and MRI,etc.How to protect the parotid glands becomes a hot research in recent years.At present,the main methods of protecting the parotid glands includes selecting patterns of radiotherapy,radiotherapy planning optimization,drugs,stem cell transplantation and gene transduction.

Objective To study the significance of thyroid stimulating antibody (TSAb) and thyroid stimulating-blocking antibody (TSBAb) in children with Graves' disease (GD) or Hashimoto's thyroiditis (HT).Methods Five hundred and twenty-seven cases of serum from 180 children with autoimmune thyroid disease (AITD) children were divided into 282 cases of GD and 245 cases of HT.According to the status of thyroid function,they were divided into 157 cases of hyperthyroidism,91 cases of hypothyroidism and 279 cases of normal thyroid.GD group was subdivided into 127 GD hyperthyroidism and 155 GD remission;HT group was subdivided 30 HT hyperthyroidism,124 HT remission and 91 HT hypothyroidism.Seventy-nine healthy children were taken as the healthy control group.Free triiodothyronine(FT3),free thyroxine(FT4) and sensitive thyroid stimulating hormone(TSH) were detected by chemoluminescence.Serum TSAb and TSBAb were detected by serum TSAb or TSBAb enzyme linked immunosorbent assay (ELISA),respectively.The differences in TSAb and TSBAb among each group were compared and analyzed of find out the relationship between TSAb and TSBAb was performed.Beside,the correlation between TSAb and TSBAb with FT3,FT4,and TSH were analyzed.Results (1) TSAb levels were significant (F =11.995,all P =0.000):the GD group (0.727 ± 0.157) ＞ HT group (0.605 ± 0.148) ＞ healthy control group (0.350 ± 0.105);the difference was significant(F =109.165,P =0.000) among hyperthyroidism group (0.745 ± 0.169) ＞ normal thyroid group (0.647 ± 0.153) ＞hypothyroidism group(0.612 ±0.144) ＞healthy control group (0.350 ±0.105);the difference was significant(F=156.712,P =0.000) in the GD hyperthyroidism group(0.747 ±0.17) ＞ GD remission group (0.640 ± 0.16) ＞ healthy control group (0.350 ± 0.105);the difference was significant (F =109.165,P =0.000) in the HT hyperthyroidism group(0.739 ±0.140) ＞HT remission group(0.655 ±0.135) ＞ HT hypothyroidism group(0.612 ± 0.140) ＞healthy control group (0.350 ±0.105).(2) TSBAb levels were significantly different(F =15.610,P =0.000):the HT group(0.704 ±0.633) ＞ GD group(0.567 ±0.178) ＞ healthy control group (0.334 ±0.104);the difference was significant(F =13.311,P =0.000) in the hypothyroidism group (0.693 ± 0.125) ＞ remission group (0.648 ±0.446) ＞hyperthyroidism group(0.562 ±0.181) ＞healthy control group(0.334 ±0.104);the difference was significant(F =19.269,P =0.000) in the GD remission group (0.672 ±0.572) ＞ GD hyperthyroidism group (0.550 ± 0.187) ＞ healthy control group (0.334 ± 0.104);HT hypothyroidism group (0.693 ± 0.725) was higher than HT hyperthyroidism group(0.618 ±0.142) and HT remission group (0.619 ±0.199),the difference was not significant between HT hyperthyroidism group and HT remission group(F =12.208,P =0.000).(3) TSAb level was positively correlated with TSBAb,FT3 and FT4(r =0.162,0.091,0.194,all P ＜ 0.05) and was negatively correlated with TSH (r =-0.224,P ＜ 0.05).TSBAb levels were negatively correlated with FT3 (r =-0.155,P ＜ 0.05) and was positively correlated with TSH (r =0.131,P ＜ 0.05).Conclusions Thyroid function was related to the serum levels of TSAb and TSBAb.TSAb and TSBAb could be regarded as an important predictive index for children with AITD during the treatment period.

Objective To investigate the expression and significance of survivin and p63 in diffuse large B-cell lymphoma (DLBCL). Methods Immunohistochemical staining was used to detect the expression of survivin and p63 in the lymphnode tissues from DLBCL patients and lymph node reactive proliferation.Results The positive expression rate of p63 and survivin proteins in DLBCL patients was 63.5 %(33/52) and 76.9 %(40/52), respectively. p63 was not expressed in lymph node reative proliferation. The positive expression rate of survivin in lymph node reactive proliferation was 20.0 %. The expression rates of survivin and p63 between two groups was significant different. The replase rate of groups with positive expression of survivin and p63 were higher than that of the negative groups. The mean survival in groups with positive expression of survivin and p63 were shorter than that of the negative groups. Conclusion Survivin and p63 are useful in therapeutic and prognostic assessment. Patients with poor prognostic can be screened out in the early period of treatment using expression of survivin and p63, which may help to improve the outcome of diffuse large B-cell lymphoma.

Objective To survey the quantity of clinical practice of medical trainees in practice,analyze the status characteristics and main problems,and put preference for practice teaching administrative departments.Methods In June 2016,all students finishing internship in some military medical university were gathered and surveyed by the person in charge.Using self-made questionnaires,the students were asked to anonymously fill out the quantity of clinical practice by themselves respectively in different department and in various capacities.515 questionnaires were provided and 479 valid results were collected (93.0％).Using SPSS 16.0,the number of trainees in different genders,specialties and categories was analyzed by variance analysis and SNK test (22 comparison).Results The numbers of clinical practice as first assistants and second assistants in surgical medicine were the most,which were 5.70 and 5.29 in average,while those in specialty medicine were the least (al1＜2.85 in various capacities).The quantity of clinical practice in different specialties and sorts as second assistants in intemal medicine showed statistical significance (F=2.646,F=2.682;P=0.008,P=0.031) and SNK test showed that those in navy general practice medicine (5.81) and soldiers (4.59) operated more.The quantity of clinical practice in different specialties and sorts as first assistants in special medicine showed statistical significance (F=2.166,F=2.765;P=0.029,P=0.027) and SNK test showed that those specialties in pharmacy (3.33) and clinical medicine (3.22) and soldiers (3.03) operated more.Conclusion The quantity of clinical practice of medical trainees has characteristics with surgical medicine most and there is no links with genders.In the departments with more opportunities,the differences among different groups are not obvious,while in the less operating departments,the students with higher motivations operate more.Therefore,these departments should focus on mobilizing the enthusiasm of students.Besides,the quantity of clinical practice in medical trainees should be looked upon objectively and the operations should be done under the guide of the teachers and on the basis of considering the security of patients.