1.Influence factors on the cryosurvival rate of post-thaw spermatozoa from sperm donors.
Liang ZHOU ; Zhou ZHANG ; Juan-Zi SHI ; Xiao-Hua LIU ; Wen-Hao SHI ; Hui KOU ; Juan SONG
National Journal of Andrology 2014;20(6):523-526
OBJECTIVETo investigate the influence of seasons, blood types and semen parameters on the cryosurvival rate of frozen-thawed spermatozoa from sperm donors.
METHODSWe retrospectively analyzed the influence of seasons, blood types, abstinence period, semen volume, sperm morphology, and pre-freeze sperm motility and concentration on the cryosurvival rate of frozen-thawed sperm in 4 088 semen samples from Shaanxi Human Sperm Bank.
RESULTSThe cryosurvival rate of the post-thaw sperm was correlated negatively with the progressive motility of the pre-freeze sperm (r = -0.262, P < 0.01), but positively with pre-freeze sperm concentration (r = 0.247, P < 0.01), and it was significantly higher in the 6-day abstinence group ([70.2 +/- 5.4]%) than in the other abstinence groups (P < 0.01), so was it in the normal sperm morphology rate > 20% group ([71.4 +/- 5.1]%) than in the others (P < 0.01). The survival rate of the post-thaw sperm was not correlated with seasons, blood types, and semen volume (P > 0.05).
CONCLUSIONSperm motility, concentration and morphology and the abstinence period of sperm donors may be valuable predictors of the cryosurvival rate of post-thaw sperm, but no correlation was found between the survival rate and seasons, blood types and semen volume.
Adult ; Cryopreservation ; Humans ; Male ; Retrospective Studies ; Semen Preservation ; Sperm Motility ; Spermatozoa ; Young Adult
2.Conjugated effects of fluoride and aluminium on rat whole blood zinc, iron, calcium, magnesium and copper level
Fei, MO ; Shu-hua, XIA ; Shi-jun, WANG ; Mao-juan, YU ; Hua, CUI ; Ji-rong, CHEN ; Hua-mei, CAO
Chinese Journal of Endemiology 2008;27(5):488-490
Objective To investigate the effect of excessive fluoride,aluminum on Zn,Fe,Ca,Mg,Cuin rat blood.Methods Forty eight SD rats were randomly divided into 4 groups matched with their weights:control group,high aluminum group,high fluorine group and high fluorine-aluminum group.Aluminum content in their drinking water was 0,90,0,90 mg/L respectively.Fluorine content of their feed was 5.2,5.2,106.0,106.0 mg/kg and aluminum Was 6.8,6.8,19.7,19.7 mg/kg respectively.90 days later,the level of blood Zn,Fe,Ca,Mg, Cu Was detected by the atomic absorption spectrometry.Results Compared among these groups,Zn,Fe,Mg and Cu content of the whole blood had significant difierences(F=46.25,14.74,6.10,2.93,P<0.05),while Ca content of the whole blood did not significantly change(F=2.81.P>0.05).Factorial analysis showed that excessive intake of aluminum could significantly decreased Zn,Fe,Mg content of the blood(F=42.66,5.41,7.04,P<0.05)and excessive intake of fluorine could significantly decreased Zn,Fe,Mg,Cu content of the blood(F=64.50,37.90,9.75,6.74, P<0.05).The coexistence offluorine and Muminum had interaction to the level of Zn(F=31.59,P<0.05)and did not obviously interact with other elements(F=0.91,1.63,1.51.0.00,P>0.05).Compared with the control group [(131.30 ±13.86)μmol/L,(10.24 ±1.02),(1.71 ±0.19)mmol/L,(20.43 ±4.42)μmol/L],Zn content in the high aluminum group[(90.84±9.98)μmol/L]decreased significantly(P<0.05),so did Zn,Fe,Mg content in tlle high fluorine group[(85.85 ±10.92)μmol/L,(8.49 ±0.68),(1.52 ±0.13)mmol/L],the difference being statistically significant(P<0.05)0Zn,Fe,Mg,Cu content in the high fluorine-aluminum group,being(82.82 ±11.00)μmol/L, (8.16±0.45),(1.46±0.09)mmoL/L,(15.69±2.38)μmol/L,respectively,all decreased signitlcarIdy(P<0.05). Compared with the high aluminum group[(9.43±1.09)mmol/L],Fe content of the high fluorine aluminum group[(8.16±0.45)mmol/L]decreased significantly(P<0.05).Conclusions Excessive fluoride can cause blood zn, Fe,Mg,Cu decline,so can excessive aluminum.Combination of excessive fluofine and aiuminum has 8ignificant synergic effect on the level of Zn but have rio influence on Ca.
3.Briefly analysis on academic origins of traditional Chinese medicine dispensing.
Xue-Min ZHAO ; Xiao-Juan ZHANG ; Hua-Qiang ZHAI ; Shi-Yuan JIN
China Journal of Chinese Materia Medica 2014;39(8):1530-1533
Through collecting and collating the development process of traditional Chinese medicine dispensing, the development of modern Chinese medicine dispensing on the basis of experience could be promoted. "Heyaofenji", "Hehe", " Heji" in ancient Chinese medicine, herbal medicine literature and law were collected, and then things were sorted out according to traditional Chinese medicine dispensing theory, skills and legal norms. Firstly, "Tang Ye Jing Fa" is the earliest book which marks the rudiment of traditional Chinese medicine dispensing. Secondly, traditional Chinese medicine dispensing theory formed in "Shen Nong's herbal classic". Thirdly, Zhang Zhongjing's "Treatise on Febrile Diseases" marked the formation of Chinese medicine dispensing skills. Lastly, Provisions in Tang Dynasty law marks the development of traditional Chinese medicine dispensing.
Chemistry, Pharmaceutical
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education
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history
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legislation & jurisprudence
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methods
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China
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Dosage Forms
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Drug Compounding
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history
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methods
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Drug and Narcotic Control
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history
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legislation & jurisprudence
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Drugs, Chinese Herbal
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chemistry
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History, Ancient
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Medicine in Literature
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Medicine, Chinese Traditional
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history
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methods
4.The nuclear localization of Y-box binding protein-1 correlates with P-glycoprotein expression in diffuse large B cell lymphoma.
Lei-lei ZHOU ; Wen-lin XU ; Ru-juan QIN ; Hua-rong TANG ; Hui-ling SHEN ; Yang SHI
Chinese Journal of Pathology 2007;36(5):329-330
ATP-Binding Cassette, Sub-Family B, Member 1
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metabolism
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Adolescent
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Adult
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Aged
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Aged, 80 and over
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Cell Nucleus
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metabolism
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Cytoplasm
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metabolism
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Lymphoma, Large B-Cell, Diffuse
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metabolism
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pathology
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Male
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Middle Aged
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Neoplasm Invasiveness
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Neoplasm Staging
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Prognosis
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Proliferating Cell Nuclear Antigen
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metabolism
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Y-Box-Binding Protein 1
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metabolism
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Young Adult
5.Problems and countermeasures for communication equipment supportability of newly established field medical unit
feng Lei SHI ; zhao Feng FU ; hua Wei HE ; Juan LUO
Chinese Medical Equipment Journal 2017;38(11):118-120
Objective To explore the problems and countermeasures for the communication equipment supportability of newly established field medical unit. Methods The characteristics of the communication equipment assigned to the field medical unit were introduced, and the problems of the equipment were summarized during 2-a exercise, examination, training and etc of the field medical unit, then some countermeasures were put forward accordingly. Results The communication equipment had problems in low utilization rate, training, operation procedure and safety, and the countermeasures were brought out from the aspects of emphasis from the director, personnel training, technical performances of the communication equipment and etc. Conclusion The communication equipment needs improving to support the commanding system of the field medical unit although it behaves better than the original equipment.
6.Conjugated effects of fluorine and aluminum on the sex hormones of male rats
Shu-hua, XIA ; Shi-jun, WANG ; Mao-juan, YU ; Jing, JIANG ; Cheng, WANG ; Fei, MO ; Ting-ting, XIE ; Yan, ZHANG
Chinese Journal of Endemiology 2008;27(2):134-136
Objective To observe the combined poisonous effects of fluoride and aluminum on sex hormone of male rats.Methods Sixteen weaned SD healthy male rats aged two week were selected and divided into control group,aluminum group,fluoride group,fluorine-aluminum group,four rats in each group.All rats in the experimental groups were fed with corn collected from the prevailng areas containing different fluorine contents respectively for 90 days.Serum testosterone(T)and estradiol(E2)were detected.Results Compared separatelv with the control group[(3.317±0.635)μg/L],serum T level of fluorine-aluminum group[(15.994±6.558)μg/L]was higher(P<0.05),but aluminum[(8.134±3.134)μg/L]and fluorine[(1.868±0.367)μg/L]groups had no significant differences(P>0.05).Compared separately with the control group[(0.319±0.072)nmol/L],E2 level of the fluorine group[(0.172±0.030)nmol/L]being lower(P<0.05),and it was not significant differences(P>0.05)in the control group when compared with aluminum group[(0.282±0.012)nmol/L],and fluorine-aluminum group[(0.265±0.047)nmol/L].Fluorine and aluminum interacted with each other(F=9.82,P<0.05).Conclusion The combined poisonous effects of fluorine and aluminum may influence sex hormone levels of male rats.
7.Progress in study of selective ERβ ligands.
Jin-ya CAI ; Jun-hao LI ; Shi-hui DING ; Juan ZHANG ; Gui-xia LIU ; Wei-hua LI ; Yun TANG
Acta Pharmaceutica Sinica 2015;50(6):658-667
Estrogen receptors (ERs) are members of nuclear receptors and related to several diseases such as cancer, inflammation and osteoporosis. ERs have two forms, ERα and ERβ, which have different functions and organism distributions. Compounds selectively targeting ERβ can regulate important physiological functions and avoid the side effects caused by targeting ERα. Therefore, selective ERβ ligands have received considerable research interest in recent years. In this article, different kinds of selective ERβ ligands were summarized and their structure-activity relationships were also analyzed.
Estrogen Receptor beta
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chemistry
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Humans
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Ligands
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Structure-Activity Relationship
8.Frequency and significance of CD4~+CD25~+FOXP3~+T regulator cells in the peripheral blood of systemic lupus erythematosus patients
Ji-Lin MA ; Long CAI ; Hua-Ping SHI ; Pu WANG ; Jian-Ning YU ; Xiao-Juan TAO ; Song-Guo ZHENG ;
Chinese Journal of Rheumatology 2003;0(11):-
Objective To investigate the frequency of CD4~+CD25~+FOXP3~+Treg cells in the peripheral blood of systemic lupus erythematosus(SLE)patients and its association with disease activity.Methods Pe- ripheral blood mononuclear cells(PBMC)from 28 patients(including 18 active SLE)and 22 healthy controls were counted and stained for CD4,CD25 and intracellular FOXP3.Cells were examined by 3-color staining on the Epics XL-MC and data were analyzed using EXPO32 software.Disease activity was assessed by systemic lupus erythematousus activity index(SLEDAI).Results The frequency of CD4~+CD25~+FOXP3~+Treg cells was significantly decreased in patients with active SLE compared with patients with inactive SEE and controls [(1.08?0.43)%,(1.58?0.45)% and(1.66?0.34)%,P
9.ECG characteristics ,preventive and therapeutic strategy of ventricular arrhythmias induced by second-ary hypokalemia
Wei ZHANG ; Shi-Juan LU ; Jiang-Hua ZHONG
Chinese Journal of cardiovascular Rehabilitation Medicine 2018;27(3):343-346
Objective :To explore related clinical manifestations ,ECG characteristics and therapeutic strategy of ven-tricular arrhythmias induced by secondary hypokalemia .Methods :Clinical data of 184 patients ,who were diagnosed as ventricular arrhythmias induced by secondary hypokalemia in our hospital from Jan 2013 to Nov 2015 ,were retro-spectively analyzed .ECG characteristics and clinical manifestations were analyzed ,blood potassium concentration and incidence of ventricular arrhythmia were compared between before and after treatment .According to blood po-tassium concentration ,patients were divided into mild hypokalemia group (n=91 ,3.01~3.5mmol/L ,mild group) , medium hypokalemia group (n=78 ,2.51~3.00mmol/L ,medium group) and severe hypokalemia group (n=15 ,<2.5mmol/L ,severe group).ECG condition was compared among these groups .Results : Main clinical manifesta-tions included palpitations ,chest tightness ,fatigue ,hypotension and short of breath etc .,and severe patients can present as coma ,intestinal paralysis and syncope etc .Among ECG manifestations ,premature ventricular systole was the most frequent [75 (40.8%)] ,and the second was frequent premature atrial systole [69 (37.5%)].Compared with mild group ,there were significant rise in percentage of positive ECG changes (50.5% vs.79.5%,100%) in medium group and severe group (P=0.001 both) ,but there was no significant difference between medium and se-vere group , P=0.120. Compared with before treatment ,after treatment ,there was significant rise in blood potas-sium concentration [ (2.5 ± 1.1) mmol/L vs.(4.4 ± 1.7) mmol/L] ,and significant reductions in percentages of ventricular premature contraction (86.96% vs .25.00%) and persistent ventricular tachycardia (8.70% vs.0) in these patients ,P=0.001 all .Conclusion :Along with blood potassium level reduces ,percentage of patients with ven-tricular arrhythmias induced by secondary hypokalemia and abnormal ECG rises .Timely ECG and blood potassium examinations should be performed in these patients for timely treatment .
10.Study on the detection of inv(16) in acute myelomonocytic leukemia (M4) by flucorescence in situ hybridization method.
Ying CAI ; Jing-Hua WANG ; Jian ZHANG ; Juan-Hua SHI ; Xi JIN
Journal of Experimental Hematology 2004;12(6):748-751
To study the clinical significance of the detection of inv(16) (p13 q22) by FISH in the diagnosis and prognosis for M(4), the metaphase bone marrow cells of 6 cases M(4) which had already diagnosed by morphology were detected for CBFbeta-MYH(11) fusion gene by MYH 11 probe including dual labelled sequences and the results were compared with that of conventional cytogenetic analysis. The results showed that 4 cases inv(16) (two M(4) EO and two M(4)) were found by G banding kanyotyping test. One case M(4) had trisomy (+22) in addition to inv(16); one case M(4) had hypodiploid in addition to inv(16), which relapsed after CR 8 months. All 6 cases had inv(16) abnormal fluorescence signal. Among them case No 2 had the deletion of 16p13 showed by red fluorescence signal in addition to inv(16). It is concluded that the detection of inv(16) by FISH is of sensitivity and specificity. It is the important supplement to the conventional cytogenetic analysis. It possesses the important clinical significance in the diagnosis and prognosis of M(4) patients.
Adolescent
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Adult
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Chromosome Inversion
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Chromosomes, Human, Pair 16
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genetics
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Female
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Humans
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In Situ Hybridization, Fluorescence
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methods
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Leukemia, Myelomonocytic, Acute
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genetics
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pathology
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Male
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Middle Aged
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Reproducibility of Results