Objective To study the dilution model of the early pregnancy test strip for the quantitative detection of serumβ-hu-man chorionic gonadotropin(β-hCG)level by the Abbot AxSYM detection system and its application.Methods The detection re-sults of serumβ-hCG level in 5 612 outpatients and inpatients of the internal medicine department and the gynecology and obstetrics department during 5 years were retrospectively analyzed.In addtion,234 serum samples from the patients were collected for prelimi-narily determining the serumβ-hCG level by the early regnancy test strip and then the quantitative detection of serumβ-hCG level was detected by the Abbot AxSYM detection system.Results The retrospective analysis showed that 241 cases(4.6%)were low-age pregnant women.The qualitative screening was necessary.The screening model was successfully established.But one thing to note was that the part of serum sample needed to be taken out and put into another tube for determination.Conclusion Establishing the rational and effective dilution interpretation model of early pregnancy test strip for screening serumβ-hCG can bring the econom-ic and time benifits for the routine detection work of the Abbot AxSYM detection system.

Adult ; Humans ; Lead Poisoning, Nervous System ; etiology ; Male

Antitubercular Agents ; pharmacology ; Drug Resistance, Bacterial ; Humans ; Microbial Sensitivity Tests ; Mycobacterium tuberculosis ; drug effects ; isolation & purification ; Tuberculosis, Multidrug-Resistant ; diagnosis ; microbiology

Objective To explore the relationship between intercranial hemodynamics and nitric oxide(NO),endothelin(ET),nailfold microcirculation in patients with migraine.Methods 50 patients with migraine and 40 normal controls underwent the measurement of intracranial hemodynamics,naifold microcirculation and the level of plasma ET,NO.The results of the two groups were compared and analyzed.Results The blood flow velocity of intracranial arteries was significantly higher in the patients than that in the normal controls( P

Cellulase system contains a series of complex components.There are still some problems remained unclear in cellulase and its mechanism of hydrolyzing lignocellulosic materials and its hydrolysis kinetics,so profound study is needed.The rapid development of many kinds of new interdiscipline such as biochemistry,molecular biology and gene engineering,has further clarified the structure and function of cellulase,and the relationshi Pof its gene expression and regulation,and furthermore resulted in derivative study methods about cellulase in more aspects.Cellulase system components according to synergistic catalytic mode and the sequence of the homology amino acids similarity,summarizes traditional detection methods of enzyme components,and emphasizes on research progress of various biosensors applied in detection of cellulase activity and gene expression was introduced.

Humans ; Infant, Newborn ; Male ; Wiskott-Aldrich Syndrome ; diagnosis ; genetics ; therapy ; Wiskott-Aldrich Syndrome Protein ; genetics

Administration, Inhalation ; Adolescent ; Asthma ; therapy ; Child ; Child, Preschool ; Female ; Humans ; Inhalation Spacers ; Male ; Metered Dose Inhalers ; Nebulizers and Vaporizers ; standards ; Technology Assessment, Biomedical ; Treatment Outcome

Aged ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Male ; Middle Aged ; Phytotherapy ; Silicosis ; drug therapy ; Treatment Outcome

Aim To study the effect of high salt diet during pregnancy on the development of renal vessels in offspring rats and its mechanism.Methods Natural pregnant Sprague-Dawley rats were randomly divided into high-salt group and control group.The pregnant rats in the high-salt group were given high-salt diet of 8% NaCl content , while the control group normal diet with 1% NaCl content.In both groups, pregnant rats were given normal drinking water.After delivery, all mothers returned to normal diet and all neonatal rats were breast-fed until one month old.The adult male off springs were used as experimental animals.The vessel tone of renal interlobar arteries and electrophysiological behavior of single vascular smooth muscle cells (VSMCs) were detected respectively.Results The contractile response of renal arteries to phenylephrine(Phe) in high-salt group was stronger than that in the control group(P<0.05).The effect of protein kinase C(PKC) non selective blocker GF109203X on Phe-induced contraction in two groups also showed significant difference(P<0.05).In electrophysiology experiments, Phe inhibited high-conductance Ca2+-activated K+ channel(BK channel) currents in renal arteriolar smooth muscle cells in two groups, and the inhibitory effect was more pronounced in high-salt offsprings (P<0.05).GF109203X could eliminate the response of Phe on BK currents in both groups(P>0.05).Conclusions High-salt diet during pregnancy could increase the sensitivity of renal interlobar arterial contractile response to Phe in adult male offsprings, which is associated with PKC-mediated BK channels pathway.Maternal high-salt diet during pregnancy may increase the risk of renal vascular diseases in adult offsprings.

AIM:To investigate the findings of the eyes which were examined preoperatively by three mirror contact lens before the implantation of implantable collamer lens ( ICL) . To analysis the retinal pathological changes and to explore the clinical analysis of early diagnosis and treatment in retinopathy on fundus examination before operation.
METHODS:The retrospective case series study included 127 eyes of 64 patients who underwent phakic intraocular lens implantation were received the fundus examination by three mirror from April 2011 to April 2012 in our hospital. The age, refractive diopter, the findings of Goldmann three mirror examination and the condition of retinal photocoagulation were analysed and concluded.RESULTS:A total of 34 eyes (26. 8%) out of all 127 eyes ( 64 cases ) were found to have peripheral retinal pathological changes. Eight eyes ( 6. 3%) with retinal holes, 15 eyes(11. 8%) with retinal lattice degeneration, 5 eyes ( 3.9%) with retina cream degeneration, 3 eyes (2.4%)with retinal paving stone degeneration,2 eyes with vitreoretinal adhesion and traction, 1 eye ( 0. 8%) with retinal hemorrhage. Twenty-five cases were given retinal photocoagulation and then received the ICL implantation after 3mo. The follow - up time was 1a. No retinal detachment happened.
CONCLUSION: Phakic before intraocular lens implantation for fundus examination by three mirror is contributed to find the peripheral retinal pathological changes and abnormity. And make the appropriate treatment before operation for improving the security of operation, it can also give help to the postoperative follow-up of the fundus of these patients.