To compare posterior choroidal thickness in high myopia amblyopia eyes at different points to high myopia and normal eyes of Chinese children and investigate the relationships between choroidal thickness, axial length and age.METHODS: Fifty Chinese children (65 eyes) with age 4~15 years ( mean 9. 91 ± 3. 41 years) were recruited. By atropine optometry they were divided into high myopia amblyopia group ( 24 eyes ) , high myopia group ( 19 eyes ) , and normal group ( 22 eyes ) . Choroidal scans were obtained for all eyes using enhanced depth imaging spectral-domain optical coherence tomography ( EDl-OCT) . Subfoveal choroidal thickness (SFCT), macular thinkness, choroidal thickness and retinal thickness at 0. 5, 1. 0, 1. 5, 2.0, 2.5, 3.0mm superior (S, 12:00 position), temporal ( T, 9:00 position) , inferior ( l, 6:00 position) , nasal ( N, 3:00 position) were measured. Meanwhile, axial lengths of all eyes were measured by A-Scan. RESULTS: Compared high myopia group and emmetropia group, SFCT and the thickness of choroids on each position were thinnest in high myopic amblyopia group, with statistically significant differences (P<0. 05). There was a significant negative correlation between SFCT and axial length in high myopic amblyopia group (r=-0. 531, R2 =0. 282, F=7. 476, P=0. 013), with no relative in age (r=-0. 292, R2=0. 085, F=2. 044, P=0. 167).CONCLUSlON: The choroidal thickness thinning in high myopic amblyopia shows a negative correlation with axial length.

·AlM:To investigate the retinal thickness change of high myopia amblyopic children, so as to discuss the relationships between the retinal thickness of central fovea of macula and the factors of axis oculi and age. · METHODS:Thirty-nine children ( 65 eyes ) with the average age of ( 9.91 3.41 ) years were recruited.All eyes were ruled out the pathological changes of fundus diseases and front section. After a tropine optometry, they were divided into three groups: high myopia amblyopic group ( 24 eyes ) , high myopia group ( 19 eyes) and normal group ( 22 eyes ) .Retinal scans were obtained for all eyes using Heidelberg optical coherence tomography ( OCT ) . Subfoveal macular thickness, retinal thickness at 0.5mm, 1.0mm, 1.5mm, 2.0mm, 2.5mm, 3.0mm superior ( S, 12∶00 position), temporal (T, 9∶00 position), inferior (l, 6∶00 position) and nasal (N, 3∶00 position) from the fovea were measured and axial length was also surveyed by A -ultrasound. Statistical analyses were performed to evaluate retinal thickness at each location and to correlate subfoveal macular thickness with axial length and age.
·RESULTS:The average subfoveal macular thinkness of the high myopia amblyopic group was thinner than high myopia group but thicker than normal group.There was no statistical difference between three groups (P>0.05). Retinal thickness inferior to the fovea at 0.5mm temporal and superior to the fovea in the high myopia amblyopic group at 1.0mm temporal were both thinner than normal group which had statistically significant ( P <0.05 ). Retinal thickness on nasal, superior, temporal, and inferior at 1.5mm, 2.0mm, 2.5mm, 3.0mm from the fovea were measured, high myopia amblyopic group were the thinnest in the three groups, and there was statistically significant between three groups ( P<0.05). There was no correlation between the average subfoveal macular thickness and axial length, age in high myopia amblyopic group.
· CONCLUSlON:There are significant abnormalities of macula retinal structure in high myopia amblyopic children.

AIM: To research the peripapillary retinal nerve fiber layer ( RNFL ) thickness change in high myopia amblyopic children and to discuss the relationships among RNFL thickness, axial length and age.
METHODS:Thirty-five Chinese children (59 eyes) with a mean age of ( 9. 59 ±2. 90 ) years were recruited. All eyes were ruled out the pathological changes of fundus diseases and front section. By atropine optometry after they were divided into: high myopia amblyopia group (22 eyes), high myopia group (15 eyes), normal group (22 eyes) . RNFL scans were obtained for all eyes using optical coherence tomography and axial length was also surveyed by A - ultrasound. Statistical analyses were performed to evaluate RNFL thickness at each location with axial length and age.
RESULTS:The peripapillary RNFL thickness in temporal of high myopia amblyopia group was thinner than that in
high myopia group, and thicker than that in normal group. The peripapillary RNFL thickness in nasal, superior, inferior and the average thickness of high myopia amblyopia group were thinner than those in high myopia and normal gruops. The peripapillary RNFL thickness in inferior and average thickness of high myopia amblyopia group were significantly thinner than those of high myopia (P<0. 05). The peripapillary RNFL thickness in nasal, superior, inferior and the average thickness of high myopia amblyopia group were significantly thinner than those of normal (P<0. 01). The peripapillary RNFL thickness in temporal of high myopia group was significantly thicker, and in nasal, superior, inferior and the average thickness were significantly thinner than those of normal (P<0. 05). The thickness of peripapillary RNFL in inferior showed a negative correlation with axial length in high myopia amblyopia group (R=0. 474, R2=0. 225, F=4. 933, P=0. 040). The thickness of peripapillary RNFL in superior showed a negative correlation with axial length in high myopia group (R=0. 642, R2=0. 412, F=9. 104,P=0. 010). These were no correlation between the peripapillary RNFL thickness and age in high myopia amblyopia, myopia amblyopia and normal.
CONCLUSION:There are significant abnormalities of retinal structure in high myopia amblyopia.

Objective: To investigate the factor structure of the PTSD through analyzing the factor structure of the PTSD Checklist in adolescents from earthquake disaster region. Methods: Chinese PTSD Checklist-Civilian Version was used to assess 560 adolescents, and technique of Confirmatory Factor Analysis (CFA) was employed to comparing seven competitive models. Results: The intercorrelated four-factor model was the best fit one. Conclusion: The Chinese PTSD Checklist-Civilian has an intercorrelated four-factor model; intercorrelated four-factor model of the PTSD may fit the Chinese people, however, which needs further confirmation.

ObjectiveTo investigate the current situation of immunophenotyping of acute leukemia by flow cytometry in China (except Taiwan, Hongkong and Macau), and to provide theoretical evidence for the formulation of guidelines of immunophenotyping of leukemia by flow cytometry.MethodsThe Chinese Hospital Knowledge Database(CHKD) was used to retrieve the1994-2009 published papers on immunophenotyping of acute leukemia by flow cytometry.All of 380 retrieved papers were analyzed by the GraphPad Prism 4. ResultsThe number of published papers in China increased yearly and changed gradually from large cities to small and medium-sized cities in local distribution. The most often reported area was Beijing, followed by Jiangsu and Guangdong. In specimen processing, the ratios of using mononuclear cell labling method and whole blood labling-lysing-washing method were 34.2 ％ (113/330) and 65.8 ％ (217/330) respectively. The ratios of using single color, two color, three color and four color labling method were 15.4 ％ (53/344), 13.7 ％ (47/344), 54.1％ (186/344) and 16.3 ％ (56/344), respectively. The number of fluorescent antibodies was used from less than 10 to more than 20.Most of them were between 11 to 16,which was 48.6 ％ (167/344) of total number of published papers. In data acquisition and analysis, the ratios of using FSC/SSC gating and CD45/SSC gating were 34.2 ％ (113/330) and 65.8 ％ (217/330), respectively. There were some differences in the positive criteria of lymphatic and myeloid and cytoplasm antigens.The main positive criteria of lymphatic, myeloid antigen was ≥20 ％, which was 64.8 ％ (223/344) and 95.4 ％ (328/344) . The main positive criteria of cytoplasm antigen was ≥ 10 ％, which accounted for 61.2 ％ (156/255). Conclusion A common view has been reached in specimen processing,gating method,data acquisition and analysis in immunophenotyping of acute leukemia by flow cytometry. But there were still some difference in the number of fluorescent antibodies, panels and evaluation of positive criteria of antigens.

Objective: To study the content determination of atractylodin, atractylon, and β-eudesmol in Atractylodis Rhizoma by GC and characteristic spectrum in order to provide a scientific basis for the quality control. Methods: Using GC and Agilent HP-5 capillary column, taking nitrogen as carrier gas, FID as detector, temperature programming, split ratio, injection port temperature: 250℃, detector temperature: 250℃, column temperature: 130℃; The contents of atractylodin, atractylon and β-eudesmol of 25 samples between Chengde and purchased from other markets were determined by external standard method. The characteristic spectrum was set up and the similarity was analyzed by Estimating System of Similarity on the Chinese Medicine Fingerprint Chromatogram. Results: The determination method and characteristic spectrum by GC for atractylodin, atractylon, and β-eudesmol in Atractylodis Rhizoma were established. Nine characteristic peaks were identified; The linear range of β-eudesmol was 20.00 - 406.10 μg/mL (r = 0.999 9), and the average recovery was 100.75%, RSD = 1.17% (n = 6), and the limit of detection was 0.12 ng. The linear range of atractylon was 35.00 - 348.70 μg/mL (r = 0.999 5), and the average recovery was 99.84%, RSD = 1.29% (n = 6), and the limit of detection was 0.04 ng; The linear range of atractylodin was 16.46 - 329.30 μg/mL (r = 0.999 6), and the average recovery was 100.12%, RSD = 0.88% (n = 6), and the limit of detection was 0.06 ng. Conclusion: The concent determination and characteristic spectrum method of atractylodin, atractylon, and β-eudesmol established by this study are sensitive, simple, stability, which could make the determination result accurate and reliable.

Objective To explore the clinical efficacy and value of azo steen,montelukast sodium combined with levocetirizine in the treatment of chronic urticaria. Methods 150 cases with chronic urticaria admitted in department of dermatology of Hubei Zhongshan Hospital were divided into A,B and C group,each group had 50 cases. Patients in group A were received montelukast sodium and levocetirizine treatment,which in group B were received montelukast sodium and azo steen treatment,group C were received above three kinds of drug treatment. Total effective rate,adverse reaction and safety in three groups were observed and compared. Results Total effective rate of group C was significantly higher than A and B group(P<0.05), and the safety of group C were better than A and B group(P<0.05 ),too. The occurrence of adverse reaction in group C was significantly less than that in group A and B (P <0.05 ).Conclusion Azo steen,montelukast sodium combined with levocetirizine has good efficacy in treatment of chronic urticaria. It can relieve itching wheals and other symptoms in a short time,with less adverse reaction.

BACKGROUND: Preliminary experimental study found that the human amniotic mesenchymal stem cells (hAMSCs)transplantation can improve nerve injury symptoms of rats with cerebral infarction.OBJECTIVE: To observe the survival, colonization and differentiation of hAMSCs in the infarct area of cerebralinfarction rats.METHODS: Sixty Sprague-Dawley rats were randomly assigned into hAMSCs transplantation, model or shamoperation groups (n=20/group). Animal models of middle cerebral artery occlusion were produced in the model andtransplantation groups by Zea-Longa method. One day after modeling, rats in the hAMSCs transplantation groupwere given in situ transplantation of 10 μL of hAMSCs (2×106) into the damaged striatum and cortex, while those inthe model and sham operation group were given the same volume of PBS. Within 1 week after transplantation, ratneurological defects were assessed and changes in their body mass were continuously monitored. Two weeks aftertransplantation, TTC staining was used to observe cerebral infarct size, hematoxylin-eosin staining was used forpathological observation of brain tissues, and immunofluorescent staining was used to detect expression ofneuron-specific nuclear protein.RESULTS AND CONCLUSION: With time, weight loss was increased while neurologic deficit scores were graduallyreduced in the hAMSCs and model groups. Compared with the model group, the weight loss and neurologic deficitscores were lower in the hAMSCs group,; however, there was a significant difference in the neurologic deficit scoresbut not in the weight loss between the two groups. Additionally, the hAMSCs significantly reduced infarct size,attenuated pathologic injury, and decreased the number of inflammatory cells. Immunofluorescence stainingshowed that the hAMSCs were observed at 1 week after transplantation under inverted luorescence microscope,and gradually differentiated into nerve cells at 2 weeks after transplantation. In conclusion, transplanted hAMSCsmay migrate to and survive in the cerebral infarct region, and differentiate into nerve cells in situ in rats with cerebralinfarction.

To explore the necessity of teaching of elective course:Flow Cytometry and its application to clinical medicine in medical students,sixty nine medical students including sevenyear program undergraduate,master and Ph.D candidates were surveyed with a community questionnaire. More than 95% students thought it was very necessary to set up the course because they knew little about the course and it would be very helpful in their future clinical and research work.