OBJECTIVE:To establish a method for the contents determination of astragalosidesⅠ,Ⅱ,Ⅲ,Ⅳ,lobetyolin and for-mononetin in Shenqi fuzheng injection. METHODS:HPLC was performed. The detector was evaporative light scattering detector, column was Ultimate XB-C18 with mobile phase of acetonitrile-0.5% formic acid (gradient elution) at a flow rate of 1.2 ml/min, column temperature was 30 ℃,and injection volume was 10 μl. RESULTS:The linear range was 0.62-5.67 μg for astragalosidesⅠ(r=0.999 6),0.78-6.31 μg for astragalosidesⅡ(r=0.999 6),0.36-3.48 μg for astragalosidesⅢ(r=0.999 7),0.81-6.72 μg for as-tragalosides Ⅳ(r=0.999 5),0.82-7.03 μg for lobetyolin(r=0.999 8)and 0.58-6.62 μg(r=0.999 7)formononetin;limit of quan-titation was 1.21,0.15,0.12,0.03,0.12,0.17 μg/ml;limit of detection was 0.35,0.35,0.04,0.01,0.03,0.04 μg/ml;RSDs of pre-cision,stability and reproducibility tests were lower than 2%;recoveries were 95.1%-101.1%(RSD=2.0%,n=9),95.2%-100.7%(RSD=1.9%,n=9),95.8%-100.2%(RSD=1.4%,n=9),96.2%-100.6%(RSD=1.7%,n=9),96.6%-101.2%(RSD=1.4%, n=9) and 95.9%-99.5%(RSD=1.2%,n=9),respectively. CONCLUSIONS:The method is simple and accurate,and can be used for the simultaneous determination of astragalosidesⅠ,Ⅱ,Ⅲ,Ⅳ,lobetyolin and formononetin in Shenqi fuzheng injection.

Animals ; Bile ; physiology ; In Vitro Techniques ; Jejunum ; physiology ; Muscle, Smooth ; physiology ; Rabbits

Animals ; Cholagogues and Choleretics ; pharmacology ; Drugs, Chinese Herbal ; pharmacology ; Gardenia ; chemistry ; In Vitro Techniques ; Muscle Contraction ; drug effects ; Muscle, Smooth ; drug effects ; physiology ; Rabbits ; Stomach ; drug effects

OBJECTIVE: To compare the efficacy, compliance, safety and economics of standard triple therapy and 10-day sequential therapy consisting of rabeprazole, clarithromycin, amoxicillin and tinidazole for Helicobacter pylori eradication. METHODS: A total of 90 pepticulcer patients who were Helicobacter pylori positive proved by C-urea breath test (C-UBT) were randomly divided into two groups. Forty-five patients received rabeprazole-based 10-day sequential therapy and the other forty-five patients received rabe-prazole-based 7-day standard triple therapy. C-UBT was carried out 4weeks after a course of treatment to evaluate the eradication of Helicobacter pylori. The two regimens were evaluated by cost-effectiveness analysis. RESULTS: Eighty-five patients completed the study. The Helicobacter pylori eradication rates by intention-to-treat (ITT) analysis and by per-protocol (PP) analysis in 10-day sequential therapy group were higher than those in 7-day standard triple therapy group (ITT: 91.1% vs. 73.3%, P<0.05; PP: 95.3% vs. 78.6%, P<0.05). The incidences of adverse reactions (PP: 14.0% vs. 11.9%, P<0.05) were similar in the two groups. The cost-effectiveness analysis showed that the C/E value of the 10-day sequential therapy were lower. CONCLUSION: Rabeprazole-based 10-day sequential therapy is more effective for eradication of Helicobacter pylori.

Objective: To explore the predictors of the non-survivable patients with severe cerebral infarction after mechanical ventilation. Methods: The clinical data of 36 patients with acute cerebral infarction who had tracheal intubation were analyzed retrospectively. The patients were divided into survivor (n = 18) group and non-survivor group (n = 18) according to their prognosis. The observational endpoint was at day 60. Patients' Glasgow Coma Scale (GCS) score, respiratory function (blood gas pH value, oxygen partial pressure, carbon dioxide partial pressure, and oxygenation index), and the causes of death before tracheal intubation were documented. Results: The mortality at day 60 after mechanical ventilation was 50% in patients with severe cerebral infarction. The median GCS score was 3. 6 ± 1. 0 in the non-survivor group, and the median GCS score was 6.3 ± 1.2 in the survivor group before tracheal intubation. Oxygen partial pressure and oxygenation index presented to decrease in both groups before the tracheal intubation. The median oxygen partial pressure in the survivor and non-survivor groups were 69 ± 17 and 52 ± 7 mm Hg, respectively; and the oxygenation indexes were 170 ± 31 and 109 ± 34, respectively. The univariate analysis showed that the patients' GCS scores decreased before the tracheal intubation. The decrease of oxygen partial pressure and oxygenation index were the important factors for predicting mortality. The multivariate regression analysis showed that the patients' GCS score (P = 0.015) and oxygen partial pressure (P = 0.026) before the tracheal intubation were an independent factors for predicting the mortality in patients with cerebral infarction at day 60 after the mechanical ventilation. Conclusion: For patients with severe cerebral infarction, the mechanical ventilation support may contribute to decreasing the mortality, particularly in patients whose respiratory system complications have resulted in deterioration of the disease. The aggregate analysis has showed that the patients' severity of conscious disturbance and the oxygenation parameters before the tracheal intubation may contribute to predicting the prognosis.

Objective To explore the adverse effects of formaldehyde(FA)on learning and memory ability of mice and the antagonistic effect of N-acetyl-cysteine(NAC),an antioxidant.Methods Thirty-four ICR mice were randomly divided into four groups,the control(NS,n=8),treated with FA(15 mg/kg,n=9),treated with NAC(100 mg/kg,n=8),treated with FA(15 mg/kg) plus NAC(100 mg/kg,n=9),the treatment was conducted by intraperitoneal injection once a day for seven consecutive days.On the eighth day,the learning and memory ability were tested by using water labyrinth task for seven consecutive days.Results The mice in FA group behaved excited,restless and then turned to repose,moveless and clustering,but this phenomena was not seen in the other groups.There was no significant difference in the body weight of mice among groups.As for learning,latent period in the FA group [(27.15?2.66)s] was significantly longer than that in the control group [(15.83?2.82)s] and the FA+ NAC group[(14.98?2.66)s],and revealed statistical significance(P

Objective To screening up-regulated protein kinases and their inhibitors in order to provide new targets for molecular thera-py of nasopharyngeal cancer.Methods GEO database and SAM software were employed to screen the up-regulated protein kinase gene in nasopharyngeal cancer.Based on DAVID database,the regulating functions of kinases were identified.The inhibitors of up-regulated kinase genes were identified by Selleckchem database.Literature mining was used to screen the potential anti-cancer drugs.Results Totally 2360 differentially expressed genes including 21 up-regulated protein kinases (CHEK1,CHEK2,PRKDC,AURKA,VRK2,STK17A,MELK,NU-AK1,TRPM7,MASTL,AXL,BUB1,BUB1B,CDK4,TTK,CDC7,CASK,AKT3,TBK1 and PBK)were identified in the whole genome profi-ling (Fold Change≥2,P <0.05).The results of function analysis showed the up-regulated genes were enriched in 10 function terms such as‘protein amino acid phosphorylation’‘phosphorylation’‘phosphate metabolic process’‘mitotic cell cycle’‘cell cycle phase’‘regulation of cell cycle’,and so on.The Selleckchem database analysis showed there were 9 up-regulated protein kinases equipped with 51 inhibitors which were proved already.The results of literature mining showed that 18 inhibitors of them had a few studies (less than 10 literatures)in cancer terms,and there was a potential to become new drugs to treat nasopharyngeal cancer.Conclusion A total of 21 up-regulated protein kinases were identified,and they might promote the nasopharyngeal carcinoma by regulating functions such as the cell-cycle control pathway.Their ki-nase inhibitors may have a potential role in anti-cancer treatment,which provided a new target point for molecular therapy of nasopharyngeal cancer.

Objective: To compare therapeutic effects of clopidogrel and loading dose ticagrelor on aged patients with ST elevation myocardial infarction (STEMI).Methods: A total of 160 aged STEMI patients undergoing emergency percutaneous coronary intervention (PCI) were selected.According to random number table, they were randomly and equally divided into clopidogrel group (received loading dose aspirin and clopidogrel therapy) and ticagrelor group (received loading dose aspirin and ticagrelor therapy).Coronary TIMI flow grade, incidence rate of no-reflow after PCI, and incidence of cardiac adverse events and bleeding events in one month after PCI were compared between two groups.Results: Compared with clopidogrel group after PCI, there was significant rise in percentage of coronary TIMI flow grade 3 (71.25% vs.90.00%), and significant reduction in incidence rate of no-reflow (28.75% vs.10.00%) in ticagrelor group,P<0.05 all.Compared with clopidogrel group on one month after PCI, there were significant reductions in incidence rates of cardiogenic death (13.75% vs.3.75%), in-stent thrombosis (6.25% vs.0) and recurrent myocardial infarction (10.00% vs.1.25%), but significant rise in incidence rate of dyspnea (3.75% vs.12.50%) in ticagrelor group, P<0.05 all.There was no significant difference in incidence of bleeding events between two groups, P>0.05 all.Conclusion: Loading dose ticagrelor can effectively improve coronary blood flow, reduce no-reflow and its safety is good in aged patients with ST elevation myocardial infarction, which is worth extending.

Objective Based on protein-protein interaction network,gene modules were identified to provide new targets for molecular therapy of nasopharyngeal carcinoma.Methods GEO dataset (GSE12452)and SAMsoftware were employed to screen the differentially ex-pressed gene in nasopharyngeal carcinoma.Protein-protein interaction network was established by using String database.Based on the net-work,the gene modules were identified by using bioinformatics gene module analysis method.GO analysis was used to analyze the function of gene modules.Results In this study,2 634 differentially expressed genes were identified in nasopharyngeal carcinoma.There were 4 729 protein-protein interaction pairs among the differentially expressed genes according to the String database.We established the protein-protein interaction network based on these pairs.Seven gene modules were identified by bioinformatics methods.GO analysis results showed that the function of the gene modules including regulation of cell cycle,glycosylation,cell adhesion,oxidation reduction and so on.Conclusion There are 7 gene modules in protein-protein interaction network in nasopharyngeal carcinoma.These modules may play important roles in the progression and development of nasopharyngeal carcinoma.Our finding can provide a new sight for molecular diagnose and therapy of nasopharyngeal carcinoma.

Objective: Observe the curative effect of basic recipes for removing phlegm and activating blood circulation in experimental model of non-alcoholic fatty liver in rats.Methods: Experimental animal model of non-alcoholic fatty liver in rats were established,then treated with Erchen decoction,TaohongSiwu decoction and the combination decoction.Basic biochemical indexes of rats were tested after treatment.Results: Erchen decoction can reduce the level of liver organ coefficient,cholesterin and aspartate aminotransferase(AST)(P