Objective To explore pediatric EEG in localized region abnormity and influence factors. Methods 51 children with EEG abnormity were retrospectively analyzed. Results In this group,there are 45 children with EEG abnormity in normal situation,and 6 of 51 childrened appear abnormity in induced tests. All the 51 childrened appeal abnormai brain wave in occipital region in varying degree. Conclusion Pediatric occipital region localization abnormal EEG may be hints related to children paroxysmal disorder, Miscellaneous factors can influence children paroxysmal disorder include all kinds of etiology, pathologic physiology signs and symptoms, age and individual variation ect.

Objective To discuss the value of EEG in early diagnosis and prognosis appraisal of children with the symptoms headed by"abdominal pain ferer"take the"abdominal pain,fever"as the first symptoms of viral encephalitis.Methods 150 cases of"abdominal pain,fever"as the first symptom diagnosed of viral encephalitis children were chosen for the EEG analysis before and after treatment.Results EEG abnormalities 117 cases,33 cases of normal.The EEG abnormality rate of abnormality is 78% ,showed diffuse or localized slow-wave number.There are two groups of children with temporal epileptiform discharges occur.There are 107 cases of abnormal EEG returned to normal and another 10 cases significantly improved in the 117 cases after symptomatic treatment of 1～2 weeks.Conclusion The EEG inspection is help with"abdominal pain,fever" as the first symptoms of viral encephalitis in children with early diagnosis,symptomatic treatment and prognosis.

Objective To observe the clinical therapeutic effect of Bushen Huoxue Zhuyun Decoction and its influence on the endometrial growth and the follicle, and supply scientific evidence for the clinical use of Bushen Huoxue Zhuyun Decoction. Methods 90 cases of patients with anovulatory sterility of kidney deficiency syndrome were divided into two groups randomly. Treatment group (50 cases) and control group (40 cases) were given Bushen Huoxue Zhuyun Decoction and clomiphene citrate respectively. Results In the treatment group, the cured rate was 24%, markedly effective rate was 56%, effective rate was 16%, and the total effective rate was 96%. In the control group, the cured rate was 15%, markedly effective rate was 17.5%, effective rate was 25%, and the total effective rate was 82.5%. There was significant difference between the two groups by statistical analysis (P 0.05). The thickness of uterine endometrium of pre-treatment and post-treatment in treatment group was significant by statistical analysis (P

Induced pluripotent stem cell (iPSC) is a type of pluripotent stem cell that can be generated directly from adult cells through gene reprogramme and cell dedifferentiations.The researching history and advantages of iPSC were reviewed in this paper.In addition,the application of iPSC on diabetis mellitus was also summarized and prospected.

Aged ; Cerebral Infarction ; drug therapy ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Free Radical Scavengers ; therapeutic use ; Ginkgo biloba ; Humans ; Male ; Malondialdehyde ; blood ; Middle Aged ; Nitric Oxide ; blood ; Phytotherapy ; Superoxide Dismutase ; blood

Objective To investigate the effects of dexamethasone on the airway development and vasculogenesis in cultured human fetal lung exposed to hyperoxia.Methods Human fetal lung at the pseudoglandular stage of development were cultured either in normoxia(21%O2,5%CO2)or hyperoxia(95%O2,5% CO2)for 72 hours.Dexamethasone was added into the culture medium at the concentration of 10-6 mol/L.Whereas ethanol at the same concentration was as control.Harvested tissue wss stained for pancytokeratin to I dentify epithelial cells.CD31 aS a specific antibody of endothelial cell.The effects of lung morphometry were analyzed using computer-assisted image analysis software to ealeuhte the thickness of airways,surface area occupied by airways and the distance of vessels to the nearest airway as well as the surface area of blood vessels.Results The lung architecture remained largely unchanged in normoxia for 72 hours culture,whereas hyperoxic culture of lungs resulted in significant dilation of airways and thinning of epithelium.The surface area occopied by airways increased significantly(P＜0.05).The blood vessels in interstium were more close to the airway epithelium in the hyperoxic lung(50 μm)as compared with normoxia group.Meanwhile,the proportion of the blood wessels in interstium of hyperoxia group(4.0%)Was higher than those of normoxia group (4.2%)and hyperoxia with dexamethasone(5.4%)(P＜0.05).Dexamethasone has the protective effect on lungs exposed tO hyperoxia.Conclusion Our resulta showed that hyperoxia resulted in the simple airways and the decreased bkxxl vessel density in interstitium of cultared human fetal lung.Dexamethasone has protective effect on fetal lungs exposed to hyperoxia and can promote lung mature.

Atherosclerosis is a multifactorial disease and mediated by chronic inflammation from initiation to progression. Inflammatory factors of this process, such as C-reactive protein, interleukin-6, cell adhesion molecules and tumor necrosis factor-a, not only involve in atherosclerosis-related disease process, but also indicate the risk of cardiovascular events. Inflammatory factors level plays a significant role in diagnosis, treatment and prognosis of atherosclerosis-related disease, such as acute coronary syndrome. Recent studies have shown that there is a marked inflammatory response after stenting, which plays an important role in the vascular remodeling process, especially in-stent restenosis. Therefore, it is necessary to explore the molecular mechanism and pathological changes in the process of inflammation and to study prevention and treatment of the inflammation. We believe that inflammatory processes will be new targets of therapy in preventing or treating atherosclerosis and its complications.

Objective To investigate the expression and localization of S100B protein in the placenta and umbilical cord tissue of the pregnant women at different gestational ages. Methods The placenta and umbilical cord tissues were obtained from 60 healthy pregnant women at different gestational ages:13～27 weeks (a = 20),28～36 weeks (n = 20),37～41 weeks (n = 20). The localization and expres-sion of S100B protein was detected by S-P immunochemical method and Western blotting. Results In the placenta,the S100B protein lo-cated in the trophoblast.myofibroblasts.macrophages and smooth muscle cells. The intensity of immunostaining and protein concentration in-creased with advancing gestation. In the umbilical cord,the S100B protein was found in the smooth muscle cells,myofibmblasts,amnion ep-ithelium, macrophages and monocytes. S100B piotein expression showed no significant difference in the different cells of the umbilical cord. Conclusion S100B protein expression in the placenta and umbilical cord tissues is throughout the gestation and increases with advancing gestation in the placenta.

Adult ; Faculty ; Female ; Humans ; Male ; Middle Aged ; Voice ; Voice Disorders ; epidemiology

Establishment of a hairy root culture system from the medicine plants transformed by the Agrobacterium rhizogenes has unique advantages in large scale production of secondary metabolites and provides an effective solution to the shortage of resources. Meanwhile, root-induced(Ri) plasmid is also an ideal vector for plant genetic engineering. Insect-resistant, disease-resistant genes or genes encoding key enzymes involved in biosynthesis of plant secondary metabolites can be harbored by Ri plasmid and integrated into the host plant genome for expression, thus improving the plant traits and enhancing the secondary metabolite content. Establishment of plant transgenic hairy root culture system has laid a solid foundation for regulating plant secondary metabolites content and for industrial production of pharmaceutical active ingredients by using of genetic metabolic engineering strategies. This paper reviews the latest research advances in this field and the related applications.