Gap junctions are membrane structures made of intercellular channels that allow directly exchange of material and information between adjacent cells. It is the structural basis of gap junctional intercellular communication. Gap junction is aberrant frequently in variety of cancers, which relatives with tumor genesis and metastasis. New anti-cancer drugs targeting gap junctions will be a new direction for tumor treatment.

In the past decades,more and more mutant genes that cause inherited retinal diseases have been found.Meanwhile,many naturally occurring animal models have also been found to show similar gene mutations and phenotypes as the human inherited retinal diseases,which have led to the development of a variety of therapeutic strategies for those traditionally incurable inherited diseases.These models include rod degeneration (rd) mice,such as rd1 model,rd10 model and nmf137.To compare the efficacy of these strategies,this article briefly reviews the recent progress in the treatment of the mouse models with mutations in the subunit of rod phosphodiesterase (Pde6b)gene by pharmaceutical,stem cell and gene therapy in order to find a more effective treatment for retinal degeneration.

On the basis of status and technical characteristics of rehabilitation training instrument in China, this article summarized theconcept and significance of reliability-based design, introduced the technical target of rehabilitation training instruments and the procedureof building a reliability model. The future prospect of applying reliability-based design to rehabilitation training instruments is also discussed.

Humans ; Multiple Myeloma ; complications ; Neuritis ; etiology ; prevention & control ; therapy ; Renal Insufficiency ; etiology ; prevention & control ; therapy

Objective To explore pediatric EEG in localized region abnormity and influence factors. Methods 51 children with EEG abnormity were retrospectively analyzed. Results In this group,there are 45 children with EEG abnormity in normal situation,and 6 of 51 childrened appear abnormity in induced tests. All the 51 childrened appeal abnormai brain wave in occipital region in varying degree. Conclusion Pediatric occipital region localization abnormal EEG may be hints related to children paroxysmal disorder, Miscellaneous factors can influence children paroxysmal disorder include all kinds of etiology, pathologic physiology signs and symptoms, age and individual variation ect.

Objective To explore the treatment outcomes of intensive pulsed light (IPL) in combinationwith rb-bFGF in patients with telangiectasia.Methods A total of 57 patients with telangiectasia were randomly divided into 2 groups:group A,IPL540nm (n=32);group B,IPL with rb-bFGF (n=25).The intense pulsed light with 540 nm wavelength,energy density of 14-17 J/cm2 and pulse width of 10-12 ms was used for 3-5 times at intervals of 4-5 weeks.Results Fifty-seven patients completed the Study with maximal 5 treatment sessions,100% improvement was observed in 15.62% and 64.00% at group A and group B respectively after 1-2 treatment sessions,but in 62.5% and 84% after 3-4 treatmentsessions.There was significant difference in treatment sessions between the groups A and B (P

The pathogenesis of children's primary nephrotic syndrome has not been completely clear at present.In the past few years,The polymorphism of these genes,such as angiotensin-converting enzyme gene,apolipoprotein E gene,platelet-activating factor acetylhydrolase gene,human histocompatibility leukocyte antigen gene,glucocorticoid receptor gene and cytokine gene,ect have been discovered that were significantly correlated with susceptibility,pathological progress,steroid response,disease recurrence and prognosis of primary nephrotic syndrome.This article reviewed the research progress of PNS and gene polymorphism.

Objective To analyze the risk factors of coronary heart disease(CHD) by health examination in Huizhou.Methods A total of 85 patients withCHD,were chosen from 970 health check-up subjects as research cases.The roles of 6 risk factors of CHD were analyzed.Results 970 examination,amomg the CHD group were 85 cases(8.8% ),non-CHD group( control group)338 cases(34.8% ),normal healthy individuals 547 cases(56.4% ) respectively;The age of 60-year-old,70-year-old were significantly higher than 50 years in CHD(X2=6.78,X2=7.02,P＜0.01 );The prevalence of obesity 、smoking、over-drinking 、hypertension and dyslipidemia in CHD group were higher than that in control group( t = 2.71,t = 2.803,t = 2.875,t = 2.841,t = 2.901,all P ＜ 0.01 );The index of crowding index ≥ 1 for 92.8%,≥ 3% for 41.2% in 85 cases with CHD;Analysis showed that coronary heart disease andTG ( r = 0.28,P ＜ 0.001 ),TC ( r = 0.23,P ＜ 0.001 ),SBP( r= 0.14,P = 0.011 ) were significantly positively correlated.Conclusions Dyslipidemia、hypertension、obesity 、smoking、over-drinking are the remarkable risk factors of CHD in Hui Zhou.

The survival of extremely low birth weight infants has increased significantly due to improvement in both scientific knowledge and technology.Lung protective ventilatory strategies have been used for these infants in attempts to decrease lung injury severity and the incidence of bronchopulmonary dysplasia.The modalities of respiratory support will depend on the status of birth.It is important to apply these techniques correctly to achieve adequate gas exchange and avoid ventilatory injury.Non-invasive ventilatory support shows valuable as an initial intervention after birth and is an effective alternative to endotracheal intubation or mechanical ventilatory treatment.

Objective:To explore the efficiency of super-paramagnetic iron oxide(SPIO)of different concentrations in vitro labeling human fibroblast cells as well as the influence on cellular viability,and to investigate the characteristics of magnetic resonance imaging.Methods:Respectively mix pure SPIO(concentration of iron:200 ?g/ml) and SPIO-PLL compound of different concentrations with substrate(final concentrations of iron:150,100,50,25 ?g/ml;final concentration of PLL:7.5 ?g/ml).Then incubate it with Hfbs for 12,24,48 h and 72 h.Collect the cells.Assess cellular viability with Trypan Blue Dye Exclusion Method;test iron particles distribution in cells with Prussian blue Stain;observe the positions of iron particles with optical microscope and TEM and calculate the labeling rate of SPIO;perform MR scanning to measure the signal intensity change of the cells.Results:Labeling efficiency of SPIO-PLL compound is much higher than that of pure SPIO(P0.05).Prussian blue Stain test shows there are more or less blue dyed iron particles inside cytoplasm of each labeled cells.And those iron particles concentrate in endosome and lysosome(with electron microscope).Culture solution of concentration in the range of 25～50 ?g Fe/ml is safe for labeling stem cells with SPIO,and 95%～100% of the cells will be effectively labeled in 18～24 h.And the MR scanning shows obvious reduction of signal intensity of the labeled cells.Conclusion:Human fibroblast cells can be easily and safely labeled with self-made SPIO-PLL compound.Labeling with appropriate solution(iron content of 25 ?g/ml) is highly efficient and has little influence on cellular viability,furthermore,with SPIO labeling,the signal intensity of cells scanned with MR is dramatically reduced.