Gap junctions are membrane structures made of intercellular channels that allow directly exchange of material and information between adjacent cells. It is the structural basis of gap junctional intercellular communication. Gap junction is aberrant frequently in variety of cancers, which relatives with tumor genesis and metastasis. New anti-cancer drugs targeting gap junctions will be a new direction for tumor treatment.

In the past decades,more and more mutant genes that cause inherited retinal diseases have been found.Meanwhile,many naturally occurring animal models have also been found to show similar gene mutations and phenotypes as the human inherited retinal diseases,which have led to the development of a variety of therapeutic strategies for those traditionally incurable inherited diseases.These models include rod degeneration (rd) mice,such as rd1 model,rd10 model and nmf137.To compare the efficacy of these strategies,this article briefly reviews the recent progress in the treatment of the mouse models with mutations in the subunit of rod phosphodiesterase (Pde6b)gene by pharmaceutical,stem cell and gene therapy in order to find a more effective treatment for retinal degeneration.

On the basis of status and technical characteristics of rehabilitation training instrument in China, this article summarized theconcept and significance of reliability-based design, introduced the technical target of rehabilitation training instruments and the procedureof building a reliability model. The future prospect of applying reliability-based design to rehabilitation training instruments is also discussed.

Cerebral palsy,a group of motor disorders,does serious harm to children's physical and mental health,and the majority of patients need rehabilitation through life-time.However,the traditional methods of rehabilitation medicine are mainly dependent on the rehabilitation manipulation,which brings heavy and long term burden to family and society.With the rapid development of biomechanics and rehabilitation engineering technology,a new type of the rehabilitation robot,through the integration of rehabilitation medicine,biomechanics,mechanics,electronics,computer science and robotics,and many other disciplines,combines the active and passive exercise and fits the normal movement patterns to achieve the purpose of rehabilitation.The paper,by focusing on taking the rehabilitation robot as an example,summarizes the progresses of the application of rehabilitation robot in children with cerebral palsy,to provide new ideas for the development of children's rehabilitation technology.

Objective To discuss the value of EEG in early diagnosis and prognosis appraisal of children with the symptoms headed by"abdominal pain ferer"take the"abdominal pain,fever"as the first symptoms of viral encephalitis.Methods 150 cases of"abdominal pain,fever"as the first symptom diagnosed of viral encephalitis children were chosen for the EEG analysis before and after treatment.Results EEG abnormalities 117 cases,33 cases of normal.The EEG abnormality rate of abnormality is 78% ,showed diffuse or localized slow-wave number.There are two groups of children with temporal epileptiform discharges occur.There are 107 cases of abnormal EEG returned to normal and another 10 cases significantly improved in the 117 cases after symptomatic treatment of 1～2 weeks.Conclusion The EEG inspection is help with"abdominal pain,fever" as the first symptoms of viral encephalitis in children with early diagnosis,symptomatic treatment and prognosis.

The corresponding season of the ve zang-viscera is the important content of the correspondence between man and universe theory in the Inner Canon of Huangdi,and the corresponding season of the ve zang-viscera related to several modes of calendar.With the exception of the way that combine the ve zang-viscera with calendar which make use of criss and cross or divide into ve parts on 12 months of a year,the authors believe that the newly discovered ancient ten months' calendar had been showed clearly in the Inner Canon of Huangdi.The text of the Inner Canon of Huangdi that cannot be explained well in accordance with twelve months' calendar can be explained by ancient ten months' calendar,and the ancient ten months' calendar is the basis that the ve phases theory is derived from the concept of the ve seasons.

Objective To observe the effects of long-term Mulanquan exercise on antioxidative capacity and vasodilation.To elucidate the mechanism by which Mulanquan regulates endothelial endocrine function.To provide scientific evidence for Mulanquan's regulating blood pressure,improving cardiovascular function and preventing cardi- ovascular disease.Methods Forty-nine women volunteers were divided into an exercise group and a control group. They were fifty to sixty years old.The exercise group included 27 subjects who had practiced mulanquan regularly for at least two years.The control group comprised 22 subjects who had no habit of exercise.Their levels of serum ma- londialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),catalase(CAT)and nitric ox- ide(NO)were quantitatively determined.Results①Serum MDA was lower in the exercise group than in the con- trol group,and the difference between the two groups was highly significant statistically.Serum SOD activity and GSH-Px activity in the exercise group were higher than in the control group,the differences between the two groups being highly significant statistically.There was no significant difference between the groups in serum CAT activity. The serum NO content of the exercise group was higher than that of the controls,with the inter-group difference statis- tically significant.②Serum NO content was positively correlated with SOD activity ,GSH-Px activity,and SOD/MDA, and negatively correlated with MDA content.Conclusion Long-term Mulanquan exercise can suppress lipid peroxi- dation in middle aged and aged women.It can help maintain high antioxidative capacity,increase the secretion of NO,and thus limit any decrease in endothelium-dependent vasodilatation,which will be good for regulating blood pressure and preventing cardiovascular disease.

Objective:To prepare monoclonal antibodies with high specificity against different regions of N protein and to analyze antigenic epitopes of SARS-CoV N protein.Methods: Balb/ c mice were immunized with purified different region N protein to prepare McAb by hybridoma technique and select by indirect ELISA.Both of these McAbs were characterized for their antibody titre in the culture supernatant as well as ascites,class,subclass and affinity analysis.The antigen specificity for McAb were evaluated and confirmed by Western blot.The binding site and capability of McAb were observed by ELISA additive assay.Results: Seven hybridoma cell lines secreting monoclonal antibodies against SARS-CoV N1 and two hybridoma cell lines secreting monoclonal antibodies against SARS-CoV N2 were obtained.About the immunoglobulin subclass of McAb,six were IgG1,two were IgG2b and one was IgG3.Western blot showed that the McAbs reacted specifically with SARS-CoV N protein.ELISA additive assay indicated that two antibodies against SARS-CoV N1 recognized same epitopes,while others recognized distinct epitopes.Conclusion: We successfully obtained nine specific McAbs against the different region SARS-CoV N protein after expression and purification of the different part SARS-CoV N protein.It shows that the N1 McAb recognizs the N-terminus of the N protein whereas the N2 McAb recognizs the C-terminus.

Objective To observe the clinical therapeutic effect of Bushen Huoxue Zhuyun Decoction and its influence on the endometrial growth and the follicle, and supply scientific evidence for the clinical use of Bushen Huoxue Zhuyun Decoction. Methods 90 cases of patients with anovulatory sterility of kidney deficiency syndrome were divided into two groups randomly. Treatment group (50 cases) and control group (40 cases) were given Bushen Huoxue Zhuyun Decoction and clomiphene citrate respectively. Results In the treatment group, the cured rate was 24%, markedly effective rate was 56%, effective rate was 16%, and the total effective rate was 96%. In the control group, the cured rate was 15%, markedly effective rate was 17.5%, effective rate was 25%, and the total effective rate was 82.5%. There was significant difference between the two groups by statistical analysis (P 0.05). The thickness of uterine endometrium of pre-treatment and post-treatment in treatment group was significant by statistical analysis (P

Objective To observe changes in plasma level of brain natriuretic peptide ( BNP), left ventricular function in acute myocardial infarction (AMI) patients and discuss the effect of atorvastatin in these patients. Methods Eight patients with acute myocardial infarction hospitalized from October 2006 to December 2008 in our department were enrolled in the study. The AMI patients were further randomly assigned into the conventionsl treatment group ( n = 38 ) and atorvastatin treatment group ( n = 42 ). In the conventional group all patients took ACEI, beta-blocker, nitrate drugs, aspirin and low molecular beparin as their conventional therapy without medication taboo. In the atorvastatin group all patients took atorvastatin 20 mg per day for three months in addition to the conventional treatment. Twenty healthy individuals were testified by coronary arteriongraphy and ultrasonic cardiogram selected as controls ( n = 20). LVEDd, LVEF and left ventricular fractional shortening (LVFS) of 80 patients with AMI and 20 healthy controls were examined by ultrasonic cardiography and their plasma concentrations of BNP were measured. As to the conventional and atorvastatin group, the parameters were tested on the day of hospitalization (within 24 hours of AMI) ,also at the 3rd week and 3rd month after treatment. All data were analyzed statistically by SPSS12. 0. Results There were no significant changes in LVEDd, LVEF, LVFS after 3-week treatment compared to pre-treatment ( P ＞ 0.05 ), but significant changes after 3-month treatment ( P ＜0. 01 ) in 2 AMI groups. Moreover, LVEDd, LVEF, LVFS in the atorvastatin group were significantly greater than the conventional group (P ＜ 0.05). The plasma levels of BNP measured within 24 hours of AMI onset in two treatment groups were significantly higher than the control group. The plasma BNP level decreased significantly after 3 - week and 3-month treatment( all P value ＜0. 01 )in both treatment groups and atorvastatin group showed even lower BNP level with comparison to the conventional group( P ＜0.05 ). The plasma BNP in myocardial infarction patients after three months of treatment were negatively correlated with LVEF( r1 = -0.48 ,r2 = -0.58 ,P ＜ 0. 01 ) which implied a close relationship between BNP and heart function. Conclusions The changes of plasma BNP is earlier than that of heart structure. The long-treatment usage of atorvastatin may improve cardiac function, decrease the plasma BNP and convert ventricular remodeling,which benefit patients besides lipid managing. The plasma BNP could be used as a sensitive indicator of the treatment response and a useful index for evaluating the heart function and pronosis in AMI patients.