OBJECTIVE: To compare specific primer method and r andom amplified polymorphic DNA (RAPD) method for identification of Velvet Antler, thus to choose a simpler method. METHODS: DNA samples were extracted from sika deer antler, wilddeer antler, reideer pilose antler and commercially available Velvet Antler, and then purified and amplified with specific primer amplification and RAPD amplification. RESULTS: The sika deer antler, wild deer antler, reideer pilose antler and some of the commercially available Velvet Antler all showed 313bp segment in the agarose gel with different brightness while the other commercial products did not show this segment. RAPD not only effectively showed positive and negative DNA amplification results, but also showed the difference in PCR products of sika deer antlerwild deer antler with different strip numbers and strip brightness. CONCLUSION: RAPD method is more accurate and rapid and it has wide application prospect.

Objective To evaluate the effects of pulmonary static inflation with different pressures on postoperative lung function in patients undergoing cardiac valve replacement with cardiopulmonary bypass (CPB).Methods Sixty ASA Ⅱ or Ⅲ patients,aged 26-70 yr,weighing 47-78 kg,undergoing elective cardiac valve replacement with CPB,were randomly divided into 2 groups (n =30 each):pulmonary static inflation with 5 cm H2O group (group L) and with 10 cm H2O group (group H).In L and H groups,pulmonary static inflation was performed with the pressure maintained at 5 and 10 cm H2O,respectively,after stopping mechanical ventilation during CPB.Arterial blood samples were taken before skin incision and at 1,3 and 6 h after termination of CPB for blood gas analysis.The alveolar-arterial oxygen pressure difference (D(A-a)O2),respiratory index (RI) and oxy.genation index (OI) were calculated.The indwelling time of endotracheal tube after operation and duration of ICU stay were recorded.Results Compared with group L,D(A-a)O2 and RI were significantly decreased and OI was increased at 1,3 and 6 h after termination of CPB,the incidence of OI less than 300 mm Hg was decreased (P ＜ 0.05),and no significant change was found in the indwelling time of endotracheal tube after operation and duration of ICU stay in H group (P ＞ 0.05).Collusion Pulmonary static inflation with 10 cm H2O can better improve postoperative pulmonary diffusion function than with 5 cm H2O in patients undergoing cardiac valve replacement with CPB.

Osteoclastic bone resorption and osteoblastic bone formation are coordinated as a coupled mechanism to effect the development of bone and to maintain bone homeostasis. Recently reported Eph/ephrin bidirectional signaling between osteoclasts and osteoblasts plays a pivol role in bone homeostasis and casts new light on coupling of bone resorption and bone formation, which is gaining more and more attention in researches of bone biology and bone diseases. The present article aims to address the researches on the Eph/ephrin bidirectional signaling between osteoblasts and osteoclasts with molecular constitution, mechanism of the signal transduction, biological significance and so on.

Intelligent prosthesis is going to be a research focus recently with the increment of the transfemoral amputees and the devel-opment of microelectronic technology, control technology and so on. As the fundamental part of the lower extremity prosthetics, the knee prostheses with high performance is still hard to design. This paper reviewed the development of the intelligent knee prostheses, classified the current available prostheses in the term of construction design, and discussed the drawback of their design and the trend of the further de-velopment.

Objective To investigate the effects of Shuxuetong on hemodynamics and serum sICAM-1,IL-6,TNF-α in patients with cerebral infarction.Methods A hundred patients with cerebral infarction were randomly divided into observation group and control group from Jan 2011 to Jan 2015.Fifty patients in control group were treated with conventional therapy alone and 50 patients in observation group were treated with conventional therapy combined with intravenous injection with Shuxuetong.The changes of hemodynamics were observed before and after treatment and the levels of serum sICAM-1,IL-6,TNF-α were detected by enzyme linked immunosorbent assay (ELISA).Results No statistical significance was found in hemodynamics of control group before and after treatment (P ＞0.05).The indexes of hemodynamics in observation group were significantly improved compared with those in observation group before treatment and those in control group after treatment (P＜ 0.05).The levels of serum sICAM-1,IL-6,TNF-α were significantly reduced in two groups after treatment (P ＜0.01) and the levels of serum sICAM-1,IL-6,TNF-α after treatment were significantly lower in observation group than those in control group (P＜0.01).Conclusion Shuxuetong can effectively improve the indexes ofhemodynamics and reduce the levels of serum sICAM-1,IL-6 and TNF-α.It has a positive effect on the treatment of cerevral infarction.

Objective To detect the changes of serum integrinβ4 level during both exacerbation and clinical remission of asthmatic patients, and observe the correlation between the changes and balance of Th1/Th2,and to explore the relationship between integrinβ4 and the immune function in asthmatic patients.Methods Thirty?five cases patients who were hospitalized in the Seventh People's Hospital of Shenzhen from May 2013 to June 2015 were collected into this study.Serum interleukin?4(IL?4),interferonγ(IFN?γ),and integrinβ4 were measured with ELISA and western?blotting methods during both exacerbation and remission of asthmatic patients. Then the level of IL?4, IFN?γand integrinβ4 of the two groups were compared. Results The level of serum integrinβ4 protein in the remission stage of asthma was significantly higher than that in the acute attack period, and the difference was statistically significant(0.633±0.032 vs. 0.375±0.107,t=3.31,P<0.01).The level of ser?um IFN?γ in the remission stage of asthma was significantly higher than that in the acute attack period,and the difference was statistically significant((49.3±6.4) g/L vs. (16.7±7.2) g/L,t=3.11,P<0.05),and the level of IL?4 was (25.3±3.6) ng/L and (43.7±11.2) ng/L respectively,the difference between the two groups had sta?tistical significance(t=3.23,P<0.05).The serum level of integrinβ4 and IL?4/IFN?γratio were passively corre?lated(the acute attack period:r=0.749,P=0.05;the remission stage of asthma:r=0.745,P=0.015).Conclu?sion The serum level of integrinβ4 is passively related to IL?4/IFN?γ. Integrinβ4 may play an important role between immune function and the development in airway inflammation of asthmatic patients.

Objective To determine the clinical value of comcombined detection of serum FT 3 ,FT4 ,TSH ,TRAb ,TPOAb on outcome of women who have hyperthyroidism during pregnancy .Methods 100 patients with hyperthyroidism during pregnancy and 102 healthy pregnant women was selected in this research .Results The difference between the disease group and control group was statistically significant(P<0 .05) .Conclusion The combined detection of serum FT3 ,FT4 ,TSH ,TRAb ,TPOAb may have special impact on outcome of pregnant women .

The concept of modern medicine in treating chronic heart failure (CHF) has changed markedly in recent years. To improve the quality of life and prolong life, the treatment goal is no longer just temporary improvement of symptoms, more importantly, is to prevent and delay the occurrence and development of ventricular remodeling. Long-term chronic over-activation of sympathetic system, renin-angiotensin-aldosterone system and other neuroendocrine factors promotes myocardial remodeling, increases myocardial injury and deteriorates cardiac function. Despite short-term use can significantly improve the blood flow dynamics, long-term use of beta-adrenergic receptor stimulators and phosphodiesterase inhibitors does not prolong life, but increases the rate of sudden death caused by cardiac arrhythmia. Angiotensin converting enzyme inhibitors and beta-blockers have become the preferred drugs in treating chronic heart failure. In fact, after long-term use, beta-blockers can significantly improve ventricular remodeling, enhance ventricular function and reduce the incidence of sudden death of patients with CHF. In traditional Chinese medicine practice, short-term use of drugs for warming yang and reinforcing qi can improve symptoms of CHF, but long-term use may have adverse effects, for these medicines can stimulate sympathetic system. Early treatment with medicines of cold and cool property may be more favorable to patients with CHF, except the advanced patients who need special intervention. Eliminating heat and nourishing yin may play more active role in controlling the occurrence and development of CHF. Drugs with good efficacy and value in treating CHF may be developed from the Chinese herbal medicines with eliminating heat and nourishing yin property.

Objective To investigate the related factors of clinical stage and prognosis in the patients with endometrial carcinoma and their relation with proto-oncogene Wip1 expression level.Methods The paraffin samples of resected endometrial carcinoma in 120 cases of endometrial carcinoma in our hospital from January 2002 to January 2012 were collected as the experimental group,the samples were verified by pathology.Contemporaneous 120 samples of biopsy normal endometrial tissue served as the control group.The expression leve of Wipl were detected in turo groups.Results (1) In the Wip1 immunohistochemical staining results:Wip1 immunohistochemical staining was negative or weak in normal endometrial tissue cells,while showed pale yellow to yellowish-brown in endometrial cancer tissue.The positive expression rate of Wip1 protein in endometrial carcinoma tissue was 77.5%(93/120),which was higher than 22.5%(27/120) in normal endometrial tissue,the difference was statistically significant (P<0.05).(2)In the Western blot results of Wip1 protein in endometrial cancer tissue and normal endometrial tissue:the relative amount of Wip1 protein in endometrial carcinoma tissue was 0.635±0.023,which was significantly higher than 0.325±0.018 in normal endometrial tissue,the difference between the two groups was statistically significant (P<0.05).(3)In the real time quantitative qRT-PCR results of various samples:Wip1 mRNA expression level was higher than that in normal endometrial tissue,which were 0.628±0.053 and 0.191±0.009 respectively,the difference between the two groups was statistically significant(P<0.05).(4) The expression level of Wip1 had no correlation with age,estrogen and progesterone status,HER2,lymph node status and TNM stage,but had correlation with P53 expression level.Conclusion (1) The Wip1 expression amount is high in endometrial carcinoma and low in normal endometrial tissue.(2)The Wip1 expression level has no relation with age,estrogen and progesterone status,HER2,lymph node status and TNM stage,while has association with P53 expression level.

Objective To investigate the risk factors and prevention for shivering after intrathecal anesthesia.Methods Atotal of 1008 patients which received intrathecal anesthesia were visited after operation.Patient gender,age,ASA status,type of surgery,anesthesia method,preventive medications,local anesthetics and postanesthetic shivering were recorded.Results Age was negatively correlated with shivering (B =-0.019,P ＜ 0.05 ).Type of surgery,anesthesia method was correlated with shivering,too( P ＜ 0.05).The likelihood of shivering after combined spinal epidural anesthesia was 1.851 times than spinal anesthesia.Preventive medications were correlated with shivering(P ＜ 0.05 ).The likelihood of shivering after prophylactic use of ondansetron plus dexamethasone was 0.196 times than that without prophylactic medication.Selection of local anesthetics were correlated with shivering (B =0.443,P ＜ 0.05 ).The likelihood of shivering after use of bupivacaine was 1.558 times than that use of ropivacaine.Conclusions Risk factors for shivering after intrathecal anesthesia are patient age,type of surgery,anesthesia method,the selection of local anesthetics.Prophylactic use of dexamethasone plus ondansetron can be an effective prevention of shivering after intrathecal anesthesia.