Side population (SP) cells are highly enriched for stem cell activity and characterized by their ability to efflux the vital dye Hoechst 33342, because they express the ATP binding cassette (ABC)-dependent transporter ABCG2. SP cells can be selected from main population using flow cytometric analysis. Currently SP cells have been isolated from many tissues and organs. SP cells of different origins have some common characteristics. This article introduces the classifications, surface marker, and characteristics of SP cells.

Abscess ; pathology ; Adenoma ; metabolism ; pathology ; Adenoma, Sweat Gland ; metabolism ; pathology ; Biomarkers ; metabolism ; Breast Diseases ; pathology ; Breast Neoplasms ; metabolism ; pathology ; Female ; Fistula ; pathology ; Humans ; Keratin-7 ; metabolism ; Keratins ; metabolism ; Mucin-1 ; metabolism ; Nipples ; pathology ; Paget's Disease, Mammary ; metabolism ; pathology ; Receptor, ErbB-2 ; metabolism ; Sweat Gland Neoplasms ; metabolism ; pathology

Objective: To analyze the sagittal and vertical skeletal an d dental changes contributing to correction of Class Ⅱ division Ⅰ malocclusion and mandibular retrusion in growing children treated with Herbst appliance. Method: 27 cases of Class Ⅱ division 1 malocclusion were studied, 17 of them were treated with the domestic Herbst appliance and the other 10 case s without treatment served as the controls. Results: After 6 to 8 months (on an average of 7 months) of Herbst appliance therapy, Class II malo cclussion was corrected to Class I occlussion, overjet decreased by 7.2 mm on a n average. SNB, mandibular ramas height (Co-Go), mandibular length (Co-Pg), ma ndibular body length (Go-Pg) and the distance from Pg to OLP plane increased si gnificantly(P

Aged ; Atherosclerosis ; complications ; Blue Toe Syndrome ; diagnosis ; etiology ; metabolism ; pathology ; Cell Nucleus ; Cholesterol ; blood ; Color ; Epidermis ; Female ; Humans ; Male

Adult ; Career Mobility ; Factor Analysis, Statistical ; Female ; Humans ; Intention ; Job Satisfaction ; Male ; Personnel Turnover ; Surveys and Questionnaires

Epilepsy ; genetics ; Humans ; Mutation ; NAV1.1 Voltage-Gated Sodium Channel ; Nerve Tissue Proteins ; genetics ; Sodium Channels ; genetics

Objective To research the functional and structural change in TgAP-PswePS1 transgenic mice after intensive light exposure insult.Methods APPswe/PS1 transgenic mice at the age of 6 months old were grouped for experiments,the transgenic mice were replaced by light insult device for 6 months,while the control mice were kept in normal conditions.After 6 months light exposure,the eyes of control and experimental mice were examined with electroretinography (ERG).The retinal morphology change was investigated with H&E staining.All of the results were quantified and statistically analyzed.Results In the control group,the amplitudes of a and b wave in the rod response were (18.33 ±3.53) μV and (107.58 ± 14.72) μV,while (64.80 ±7.57) μV and (178.76 ± 14.47) μV for the amplitudes of a and b wave in the maximum response;After treated by 6 months of intensive light exposure,in experimental group mice,the amplitudes of a and b wave in the rod response were (17.92 ±4.89) μV and (21.83 ± 5.51) μV;While in the maximum response a striking decrease was detected with a wave (18.23 ±4.44) μV and b wave (24.50 ± 4.49) μV,by compared with control group,the difference were statistical significant (all P ＜ 0.05).Histopathological analysis found significant loss of outer nuclear layer,photoreceptor out segment,whereas controls remained little change in the retina.And the retinal thickness decreased significantly from (181.32 ± 13.47) μm in control group to (102.34 ±9.38) μm after light insults in experimental group,the difference was statistical significant (P =0.017).Conclusion Intensive light exposure can cause the retinal structural and functional disorder in the AP-Pswe/PS1 transgenic mouse.

Objective To investigate antiproliferative activity of endostatin(ES), the combining effect of Endostar and radiation on VEGF and apoptosis of the A549 human lung adenoearcinoma cell line.Methods The cell of exponential phase of growth were divided into the control, radiation alone, ES alone,radiation after ES(ES→RT), ES after radiation(RT→ES), radiation and ES at equal paee (RT+ES), γ-ray radiation at a dose of 2 Gy, single fracination irradiation, YH-16 10 μg/ml in the cell culture bottle. The combining effect was quantified by the survival curve. ELISA was used to observe the VEGF of radiation with ES on A549 cell lines. Apoptosis was observed by Hoeehst staining. Results There showed that both of ES and combining treatment had the function of antiproliferative. But the union treatment's function was more obvious (P＜0.05). The expression of VEGF in ES and the combining treatment group was more lower than others (P＜0.05). On apoptosis, the rate of apoptosis in ES, RT, combining treatment group was higher than others (P＜0.05). Conclusion On antiproliferative activity and rate of apoptosis, the combining of ES and radiation is better than to use them alone. To reduce VEGF of the A549 human lung adenocareinoraa cell line by ES could enhance radioresponse. There is no evidences to show that the expression of VEGF is concerned with the different choronological order of radiation and ES.

Objective To evaluate the strictness and self-consistency of the charge nurses in the nurses examination using venipunctnre trocar operation as research object.Methods FACETS,the polyhedral Rasch software was used in the examination.Results The strictness and self-consistency of charge nurses were evidently different.Conclusions The variable strictness and self-consistency of the charge nurses in the examination leads to unreliability of the results.Developing a scientific and reliable evaluation system is essential to improve the ability of the teaching nurses and the student nurses as well as the hospital nursing quality.

Objective To explore the expression of miR-9 in H/RS cells and its regulation on target PRDM1.Methods miR-9 expression in normal CD19+ B-cell subsets and eight lymphoma cell lines was detected by fluorescence quantitative RT-PCR and in situ hybridization (ISH),for quantification and location,respectively.Chemically synthesizcd antisense oligonucleotide of miR-9 was transiently transfected into L428 for its silence,and the PRDM1 expression was tested.Results Fluorescence quantitative RT-PCR showed that the expression of miR-9 in L428 cells was marked higher than that of normal CD19+ B-cell subsets and other lymphoma cell lines (the expression of miR-9 in L428 cells was 47-fold of OCI-Ly1,50-fold of Raji cells,7-fold of EBV+ immortalized B cell line,and 6-fold of ALCL cell line).ISH indicated that miR-9 located in cytoplasm,it was a diffuse and strong positive in L428,scattered and weak in DLBCL and Burkitt' s lymphoma cell lines,while negative in KARPAS-299 or Jurkat cell lines.Transient down-regulation of miR-9 in L428 leded to the increase of PRDMI protein.Conclusion miR-9 plays the role of cancer gene in cHL,and may exert a potential function in regulating terminal B cell differentiation through a post transcription regulation of PRDM1 gene.