Nuclease-based genome editing has proven to be a powerful and promising tool for disease modeling and gene therapy. Recent advances in CRISPR/Cas and TALE indicate that they could also be used as a targeted regulator of gene expression, as well as being utilized for illuminating specific chromosomal structures or genomic regions.
CRISPR-Cas Systems ; genetics ; Deoxyribonucleases ; genetics ; Gene Expression Regulation ; Genetic Engineering ; Genomics ; methods ; Humans ; RNA Editing ; genetics