Adult ; Humans ; Male ; Occupational Health Services ; Physical Examination

Antitubercular Agents ; pharmacology ; therapeutic use ; Diagnostic Techniques, Respiratory System ; Drug Approval ; Drug Resistance ; Female ; Gene Amplification ; Humans ; Immune Adherence Reaction ; Male ; Treatment Failure ; Tuberculosis ; diagnosis ; drug therapy ; genetics

Objective To observe the changes of neural stem cells(NSCs) in subventricular in developing human fetal brain at(diffe)-rent ages.Methods Thirty cases of embryoes at gestational age 24-28 weeks induced by labor with water bag were collected to determin distribution,shapes and growth modes in subventricular with hybridization in situ under light microscope.Results NSCs expressing Nestin mRNA existed in subventricular from human fetal brain at different ages,NSCs existed in subventricular of different fetal age included astro-NSCs,each had enations from 3 to 6,and all projections crowded together into a reticular plexiform,in which NSCs districuted.Nucli were round in shape,each had nucleoli from 1 to 4.NSCs had rarefaction chromatin,most NSCs existed in a single growth mode,symmetral cleavage growth mode and clony with 3 NSCs would be seen.There were no differences between positive Nestin mRNA NSCs in distribution and cell shapes,but had differences in growth mode.Conclusion NSCs exist in subventricular from different gestational ages and their growth mode are changing with difference of gestational age.

Objective To explore a precise and dependable method for determining neural stem cells(NSCs)in hippocampus from human fetal brain.Methods Ten cases of embryo at gestational age 32 weeks and induction of labor with water bag were collected,affused and sliced including frost slice and paraffin slice for determining alteration of tissue and NSCs in hippocampus from human fetal brain with HE stain and immunohistochemical staining under light microscope.Results Nestin proteins located in NSCs and the number of NSCs were less in paraffin slice of tissue than those in frost slice.Integrality of structure of tissues and cells in paraffin slice excelled in frost slice.Conclusions There is a precise and dependable method including induction of labor with water bag and allusion and frost slice,which is necessary for wide useness in study of NSCs in human fetal brain.

0.05 and P values in later four groups were

Objective To study the relationship between microwave radiation exposure during pregnancy and the offspring health. Methods The pregnant Kunming mice were divided into 3 groups and exposed to microwave radiation of 0, 570 and 1 400 ?W/cm2 respectively. The bone marrow cells of the offspring were collected for the determination of chromosome aberration (CA), sister chromatid exchange (SCE) and micronucleus frequency (MNF). Results SCE showed a significant change in high intensity group, as for CA and MNF, no significant change was seen compared with the control. Conclusion Microwave radiation exposure during pregnancy may induce germ plasm damages in the offspring.

Cerebral Hemorrhage ; epidemiology ; etiology ; prevention & control ; Cerebral Ventricles ; Humans ; Incidence ; Infant, Newborn ; Infant, Premature ; Infant, Premature, Diseases ; epidemiology ; etiology ; prevention & control

Antiphospholipid Syndrome ; pathology ; physiopathology ; Child ; Female ; Follow-Up Studies ; Humans

Animals ; Herpesviridae ; genetics ; metabolism ; Herpesviridae Infections ; virology ; Humans ; Viral Envelope Proteins ; genetics ; metabolism

Child ; Critical Care ; Humans ; Intensive Care Units, Pediatric ; Medical Records ; Severity of Illness Index