AIM: To study the effects of As_2O_3 on the growth of human cervical carcinoma cell line, HeLa cells, human pancreatic carcinoma cell line, AsPC-1 cells, and the generation of reactive oxygen species (ROS) in the cells. METHODS: HeLa cells and AsPC-1 cells were treated with various concentrations of As_2O_3. The effects of As_2O_3 on HeLa cells and AsPC-1 cells survival and apoptosis were determined by MTT assay and light microscope, and cellular ROS was also measured by fluorometer. RESULTS: After being treated with 2 ?mol/L As_2O_3 for 48 h, the survival of HeLa cells was decreased, a marked apoptosis characteristic was observed in time- and dose-dependent manner. However, the survival of AsPC-1 cells markedly decreased after being treated with even 1?mol/L As_2O_3 for 24 h. When we measured the cellular hydrogen peroxide (H_2O_2) level, we found that the H_2O_2 level in HeLa cells started to rise after being incubated with As_2O_3 for 2 h, and sustained increased, reached the peak of H_2O_2 level at 8 h, then it began to decrease. While the H_2O_2 level in AsPC-1 cells started to rise at 2 h, reached the highest point at 5 h, after which it began to descend. At 12 h, the H_2O_2 level in HeLa cells and AsPC-1 cells were similar to that of the control group. CONCLUSION: The molecular mechanisms underlying As_2O_3 -induced apoptosis in cells derived from the solid tumors may be related to the cellular ROS level.

To interpret the traditional Chinese medicine (TCM) theory by the network technology, in order to promote the modernization and programming of studies on compatibility of TCMs. In this paper, efforts were made to express the direct interactions between drugs through the herb-pair network, analyze the community characteristics of the network and its relations with blood-Qi theory, and study the expression of blood-Qi theory on the herb-pair network through prescriptions. According to the findings, the herb-pairs network showed a strong community structure characteristics; Each community is composed of a series of herb pairs with close correlations, and either blood efficacy or Qi efficacy but not both of them. Based on that, the 386 single TCM ingredients involved by the herb-pair network were divided into three types of communities: Blood (B) community, Qi (Q) community and uncertain community. According to the statistical results of 262 prescriptions mapped onto the three types of communities, if a prescription contains single herbs of the Q community, the probability that it contains single herbs o the B community is 99.84%; Meanwhile, there are 140 prescriptions containing single herbs of both the Q community and the B community. The result is completely coincident with the TCM Blood-Qi theory that single herbs belong to both Q and B communities or the B community, because Qi regulation leads to blood regulation, but not vice versa. For example, a patient with hemorrhage due to trauma or blood-heat, Qi tonifying prescriptions may aggravate hemorrhage. In this paper, authors found high-recognition macroscopic network numerical characteristics to network data reference for judging rationality of new prescriptions, and proved human blood and Qi relations from the perspective of data analysis.
Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional

Objective:To explore the moderating effect of self-differentiation between life events and coping styles. Methods:Totally 584 college freshmen from Beijing and Jiangsu were selected to fill in the Adolescent Self-Rating Life Events Check-list (ASLEC),Differentiation of Self Inventory(DSI)and Simplified Coping Style Ques-tionnaire (SCSQ). Results:Self-differentiation had a moderating effect between life events and coping styles. The predicting effect of life events on negative coping was 0. 03 in the higher self-differentiation group (P>0. 05 ) while in the lower self-differentiation group,it was 0. 27 (P<0. 00 1 ). Conclusion:It suggests that higher self-differ-entiation could relieve the negative effect of life events to coping styles.

Pressure-support ventilation (PSV) is a form of important ventilation mode. Patient-ventilator synchrony of pressure support ventilation can be divided into inspiration-triggered and expiration-triggered ones. Whether the ventilator can track the patient's inspiration and expiration very well or not is an important evaluating item of the performance of the ventilator. The ventilator should response to the patient's inspiration effort on time and deliver the air flow to the patient under various conditions, such as different patient's lung types and inspiration effort, etc. Similarly, the ventilator should be able to response to the patient's expiration action, and to decrease the patient lung's internal pressure rapidly. Using the Active Servo Lung (ASL5000) respiratory simulation system, we evaluated the spontaneous breathing of PSV mode on E5, Servo i and Evital XL. The following parameters, the delay time before flow to the patient starts once the trigger variable signaling the start of inspiration, the lowest inspiratory airway pressure generated prior to the initiation of PSV, etc. were measured.
Exhalation ; Humans ; Inhalation ; Interactive Ventilatory Support ; Lung ; physiology ; Pressure ; Ventilators, Mechanical

Objective:To compare the anti-obesity effect of green tea and black tea polyphenols and investigate their molecular mechanisms. Method:Rats were divided randomly into four groups:control group,high-fat group,high-fat diet with green tea polyphenols(GTP) supplement group,and high-fat diet with black tea polyphenols(BTP) supplement group. Body weight was determined every 2 w. After 3 months,the changes of epididymal fat tissues weight and serum lipids were observed. Expressions of those genes associated with adipocyte differentiation in epididymal fat tissues of rats were measured by real-time transcription-polymerase chain reaction,including pref-1,aP2,TNF-?,leptin,PPAR-?,C/EBP-? . Results:Both GTP and BTP prevented the increase of body weight and fat induced by high-fat diet and profoundly down-regulated those adipocyte-specific genes,including aP2,TNF-?and leptin. In addition,GTP also up-regulated the pre-adipocye marker — pref-1 and reduced the expression of transcription factor,peroxisome proliferators-activated receptor(PPAR-?) . Conclusion:Tea polyphenols could prevent obesity by reversing the adipocyte differentiation,and GTP possessed stronger inhibitory effect than BTP.

Objective To explore the risk factors and outcomes associated with pulmonary hemorrhage in very low and extremely low birth weight infants.Methods Retrospective analysis were performed to predict risk factors for pulmonary hemorrhage in very low and extremely low birth weight infants (birth weight less than 1200g) admitted to NICU of Shengjing Hospital from Jan.2010 to Dec.2015.Infants at similar birth weight without pulmonary hemorrhage were as controls.We compared the characteristics of both maternal and infants.Multivariable Logistic regression models were derived to predict pulmonary hemorrhage.Short outcomes of the infants were assessed.Results Of the 435 neonates,71 developed pulmonary hemorrhage (pulmonary hemorrhage group),364 were as controls (control group).Gestational age[(28.2±1.7)week],birth weight[(936±192)g] in pulmonary hemorrhage group were significantly lower than those in control group[(29.5±2.1)week,(1033±134)g,t=4.776,5.145,P<0.01].Neonatal respiratory distress syndrome(RDS)(76.1%),pulmonary surfactant (PS)use(PS use≥2 courses)[76.1%(9.9%)],patent ductus arteriosus (PDA)(66.2%)were significantly higher than those in control group[41.2%,30.8%(4.1%),38.7%;χ2=33.457,28.970(4.074),32.798,P<0.05].Antenatal corticosteroids utility ratio (21.1%)was lower than that in the control group (41.2%;t=10.177,P< 0.001).Multiple factors Logistic stepwise regression analysis showed that RDS (OR=3.739,95%CI 1.383-10.113,P<0.05 ),PDA (OR=2.206,95%CI 1.205-4.093,P<0.05),and 5 minutes Apgar score <7(OR=2.851,95%CI 1.191-6.828) were independent risk factors of pulmonary hemorrhage,and higher birth weight (OR=0.998,95%CI 0.996-1.000,P<0.05) and the use of antenatal corticosteroids (OR=0.432,95%CI 0.224-0.834,P<0.05) were the protection factors in pulmonary hemorrhage.In pulmonary hemorrhage group,the incidence of intracranial hemorrhage,retinopathy of prematurity and severe bronchopulmonary dysplasia(16.9%,12.7% and 18.3%) were significantly higher than those in control group (5.8%,4.4% and 2.2%;χ2=36.824,7.520 and 33.568,P<0.01);Compared to control group,the mortality in pulmonary hemorrhage group was higher (49.3% vs.14.0%;χ2=46.634,P<0.01).Conclusion Pulmonary hemorrhage in very low and extremely low birth weight infants is associated with multiple factors.Prevention of premature birth and prenatal corticosteroids treatment can help prevent the occurrence of pulmonary hemorrhage.The incidences of poor outcomes are higher in newborns with pulmonary hemorrhage.

Objective To investigate and compare the effects of PM2.5 in ambient air samples collected from nickel and nonickel contaminated areas on inflammatory factors in the serum of rats.Methods The air PM2.5 of the nickel-contaminated area and the control area were collected and detected for some major elements.Forty-two male Wistar rats were randomly divided into 10 groups,6 in each,which were respectively instilled with saline,suspended PM2.5 fluid of the nickel-contaminated area and the control area of 3 different doses(1.6,8.0,40.0 mg/kg) through the trachea.The rats were sacrificed 24 hours after being treated with PM2.5.The levels of IL-6,CRP in serum were examined.Results There was no significant difference in the level of PM2.5 between the two areas,the concentration of Ni in the nickel-polluted area was 48.75 times as that in the non-polluted area.A good dose-dependent manner was found in the test of IL-6 in nickel-contaminated groups.CRP was found significantly higher in the nickel-contaminated group and the control group than the saline control group(P

Objective To study the effects of Rhodiola herb on behavior and the expression of Bcl-2 and Bax protein in hippocamps of vascular dementia rat induced by chonic cerebral ischemia.And the protective effect of Rhodiola herb was studied.Methods VD model was formed by adopting the permanent occlusion of bilateral carotid arteries in Wistar rats to produce the forebrain ischemia.Cognitive ability of rats were studied with the Morris water maze.The expression of Bcl-2 and Bax protein in the hippocampus were determined with immunohistochemical staining.Results VD model may decrease cognitive ability(P

Objective To study the intervention effect and mechanism of integripetal rhodiola herb (IRH) on Alzheimer’s disease model rats. Methods Alzheimer’s disease model was made by injecting D-galactose (10 mg/mL) in abdominal cavity and taking AlCl3 (20 mg/mL) by intragastric administration, and injecting scopolamine. Morris water maze testing system was undertaken to observe the change of learning and memory ability in rats, biochemical indicator tested was to contrast the state of oxidative stress, common HE staining and immunohistchemistry staining were to observe the morphology change of the hippcampus and cortex cell. The model rats were administrated IRH by means of intragastric administration contrasting with hydergine to compare the effect. Results The memory ability of model group was obviously decreased, cellula nervosa were damaged badly. A? and senile plaques appeared on hippcampus and cortex. All of the test indexes in Morris water maze showed that the abilities of space search, learning and memory in different groups remarkably improved compared with that of the model group (P

Objective By comparing the biological characteristics among Lewis lung cancer cells ( LLC) , LLC or-thotopic transplantation derivative cells ( R1-LLC) and R1-LLC orthotopic transplantation derivative cells ( R2-LLC) , we e-valuate their invasion and metastatic abilities in orthotopic transplantation models.Methods In vitro, we applied CCK8, clonogenic assay, and Transwell invasion assay to detect the proliferation ability, invasion ability and morphologic structures of LLC,R1-LLC, R2-LLC cells respectively, meanwhile observing morphologic structures by transmission electron microsco-py.In vivo, we constructed LLC, R1-LLC and R2-LLC orthotopic transplantation models.Herein, we observed their tumor growth and metastasis.The tumor formation rate and tumor-forming time were recorded for statistic analysis.Results In vitro:LLC, R1-LLC and R2-LLC cells showed no significant difference in proliferation ability ( P>0.05 ) , but significant differ-ences in invasion ability:R2-LLC>R1-LLC>LLC(P<0.05).In vivo:In the orthotopic group, the tumor formation rates of LLC, R1-LLC and R2-LLC cells were 66.67%、80%、93.33%(P <0.05).Conclusions In orthotopic implantation mouse models, R2-LLC cells present higher invasion and metastatic ability than R2-LLC and LLC cells.