Adolescent ; Autonomic Nervous System ; physiopathology ; Blood Pressure ; physiology ; Blood Pressure Monitoring, Ambulatory ; methods ; Child ; Child, Preschool ; Circadian Rhythm ; Female ; Heart Rate ; physiology ; Humans ; Male ; Posture ; physiology ; Syncope, Vasovagal ; diagnosis ; etiology ; physiopathology ; Tilt-Table Test ; Vagus Nerve ; physiopathology

Widely accepted in other countries,DRG payment is also referred to in China now and then.The authors reviewed the status quo of medical payment in China,as well as the practices and studies of DRG payment overseas.Based on such studies,the paper probed into the feasibility of DRG application in China,in terms of policy environment,application demand,research basis and technical conditions.Proposed in the paper are ranges of application,possible roadblocks and countermeasures for the application in China,aiming to promote the research,application and use of the method in China.

Objective No reports has been found to date on whether frequency compounding can improve elastographic image signal to noise ratio (SNRe) and how it affects elastogram performance.In this paper simulations investigation was carried out on transmit-side frequency compounding (TSFC)for elastography.Methods 50 mm×50 mm tissue model was simulated with two round hard inclusions of 10mm diameter uniformly distributed along the tissue central axial line,and their elasticity modulus were 10 times of the background.Then simulation of 3.5 MHz、5 MHz and 7.5 MHz probes were introduced to form compression elastography of the double-lesion model by quasi-static compression method (applied strain 1%).Then,sub-elastograms obtained by the combination of 3.5 MHz and 5 MHz,3.5 MHz and 5 MHz,3.5 MHz and 7.5 MHz were compounded,respectively.Results Before compounding,signal to noise ratio (SNRe) of the various sub-elastograms were 8.42,9.62,10.73,respectively,contrast to noise ratio (CNRe) were 11.35,14.82,18.37,respectively and axial resolutions were 9.83,9.82,9.81.After compounding elastograms,the SNRe were 11.82,13.05,19.45,CNRe were 22.31,27.63,56.12,while axial resolutions were 9.83,9.83,9.83.Conclusion Frequency compounding elastograms have higher SNRe and CNRe than any sub-elastogram before compounding and have no axial resolution loss.The TSFC can improve elastogram performance efficiently and frequency compounding for elastography enhancement is feasible.

Objective To investigate trichorhinophalangeal syndrome 1 gene (TRPS-1) expression patterns in different subtypes of breast cancer and its correlations with other genes and survival using microarray data sets.
Methods The transcripts of TRPS-1 and its role in survival in breast cancer were analyzed using published microarray data sets-Netherlands Cancer Institute (NKI) cohort andWang cohort.
Results TRPS-1 expression was lower in basal-like breast cancer. The mRNA levels of TRPS-1 negatively correlated with Slug (Pearson correlation coefficient=?0.1366, P=0.0189 in NKI data set and Pearson correlation coefficient=?0.1571, P=0.0078 in Wang data set), FOXC1 (Pearson correlation coefficient=?0.1211, P=0.0376 in NKI data set and Pearson correlation coefficient=?0.1709, P=0.0037 in Wang data set), and CXCL1 (Pearson correlation coefficient=?0.1197, P=0.0399 in NKI data set and Pearson correlation coefficient=?0.3436, P<0.0001 in Wang data set), but positively correlated with BRCA1 (Pearson correlation coefficient=0.1728, P=0.0029 in NKI data set and Pearson correlation coefficient=0.1805, P=0.0022 in Wang data set). Low TRPS-1 expression associated with poor overall survival (hazard ratio 1.79, 95%CI of ratio 0.9894 to 3.238, P=0.054) and relapse-free survival (hazard ratio 1.913, 95%CI of ratio 1.159 to 3.156, P<0.05). The low TRPS-1 mRNA levels predicted poor outcome in breast cancer patients by the 70-gene signature.
Conclusion The strong expression of TRPS-1 may serve as a good prognostic marker in breast cancer.

Cardiomyopathies ; diagnosis ; etiology ; Child ; Humans

Angiopoietins ; metabolism ; physiology ; Animals ; Cell Proliferation ; Endothelial Cells ; cytology ; physiology ; Humans ; Neoplasms ; blood supply ; Neovascularization, Pathologic ; physiopathology ; Neovascularization, Physiologic ; physiology ; Pericytes ; cytology ; metabolism ; physiology ; Proto-Oncogene Proteins c-sis ; metabolism ; physiology ; Receptor, Platelet-Derived Growth Factor beta ; metabolism ; physiology ; Receptor, TIE-2 ; metabolism ; physiology ; Signal Transduction

Objective To investigate the HBV- positive pregnant women about the knowledge of the route of hepatitis B transmission, hepatitis B hazards, health care during pregnancy, announcements of child- care to explore targeted and effective health education model with self- designed question-naires. Methods Nurses in obstetrics department carried out this questionnaire investigation in the outpa-tients of HBV- positive pregnant women, after that the results underwent statistic analysis. Results The overall knowledge of HBV- positive pregnant women in announcements of child- care, health care during pregnancy and hepatitis B hazards was not very well, and the announcements of child- care was the lowest, followed by health care during pregnancy and hepatitis B hazards. The route of hepatitis B transmission was better, but still not satisfactory. The education should highlight these areas. Well- educated women were better than those who lack education. The age between 20 to 30 years old was better. Conclusions The HBV-positive pregnant women is lack of heath knowledge, so we should gave health intervention in a multi- level, multi- stage standardized and a variety of ways.

Objective To study the effects of early enriched environment on expression of growth associated protein 43(GAP-43) in hippocampus of white matter damaged(WMD) neonatal rats.Methods Two days old postnatal SD rats (n=29) were collected and randomly divided into normal control group(n=9),intervention group(n=10) and non-intervention group(n=10).The latter 2 groups were subjected to WMD at first.The right common carotid artery was dissected and ligated,and after 2 hours rest,exposed to 60 mL?L-1 oxygen 940 mL?L-1 nitrogen gas mixture.After that,intervention group received the neonatal handling and was kept in an enriched environment for 27 days.Neonatal handling was applied from the 4th day to the 10th day after WMD.Then intervention group was put into enriched environment from the 11th day to the 30th day after WMD.The other 2 groups were fed in normal environment.The abilities of sensorimotor function (hanging test and inclined plane test) were observed after intervention,and Western blot was used to examine the expression of GAP-43 in hippocampus at the 7th day and the 27th day after intervention.Results The sensorimotor function abilities of intervention group were much higher than those in non-intervention group (P

This study was to investigate the chemical constituents from pseudobulbs of Pleione yunnanensis, one of the source of traditional Chinese medicine "Shancigu". The chemical constituents were isolated by various chromatography methods, including silica gel, ODS, Sephadex LH-20, and semi-preparative HPLC. Fourteen compounds were isolated and identified from the EtOAc fraction of 90% ethanol extract, including five dihydrophenanthrenes, four bibenzyls, two triterpenoids, and three phenylacrylic acids. Their structures were identified on the basis of the spectral data as 4, 7-dihydroxy-2-methoxy-9,10-dihydrophenanthrene (1), 4, 7-dihydroxy-1-(p-hydroxybenzyl)-2-methoxy-9,10-dihydrophenanthrene (2), (2,3-trans)-2-(4-hydroxy-3-methoxyphenyl) -3-hydroxymethyl-10-methoxy-2,3,4,5-tetrahydro-phenanthro[2,1-b]furan-7-ol (3), pleionesin B (4), blestriarene A (5), batatasin III (6), 3, 3'-dihydroxy-2-(p-hydroxybenzyl) -5-methoxybibenzyl (7), 3', 5-dihydroxy-2-(p-hydroxybenzyl) -3-methoxybibenzyl (8), 3,3'-dihydroxy-2,6-bis(4-hydroxybenzyl) -5-methoxybibenzyl (9), triphyllol (10), pholidotin (11), (E) -p-hydroxycinnamic acid (12), (E)-ferulic acid (13), and (E)-ferulic acid hexacosyl ester (14). Compounds 5,10-14 were separated from this plant for the first time.
Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Molecular Structure ; Orchidaceae ; chemistry ; Spectrometry, Mass, Electrospray Ionization