The angiogenic microenvironment is a new blood vessel with different molecular and functional characteristics that sprouts on the original blood vessels through different mechanisms,which directly affects the process of tumor cell growth,proliferation,and migration and has an important impact on the occurrence and development of precancerous lesions of gastric cancer.Correa mode has shown that precancerous lesions of gastric cancer is the key pathological stage before the occurrence of gastric cancer,and it is of great significance to advance the prevention and treatment strategy to this stage.TCM believes that qi deficiency and blood stasis is the key pathogenesis of precancerous lesions of gastric cancer,and its basic treatment is to replenish qi and remove blood stasis,and based on the syndrome differentiation,drugs with the efficacy of nourishing yin and tonifying stomach,soothing the liver and regulating qi,resolving phlegm and dispersing lumps,and clearing heat and dampness for treatment.This article discussed the correlation between precancerous lesions of gastric cancer and angiogenic microenvironment and its regulatory pathways,and summarized the methods and mechanisms of TCM in the treatment of precancerous lesions of gastric cancer from the perspective of regulating angiogenic microenvironment-related pathways,in order to provide a reference for the treatment of precancerous lesions of gastric cancer with TCM.

We studied medium alkalinization in Salvia miltiorrhiza suspension cultures treated with salicylic acid and the effect of Ca2+ in this process through application of calcium channel antagonists (Verapamil, LaCl3, LiCl, 2-APB) and ionophore A23187. The results show that salicylic acid could induce significant medium alkalinization in S. miltiorrhiza culture. Verapamil and LaCl3 or LiCl and 2-APB, two different groups of calcium channel antagonist, significantly inhibited the medium alkalinization induced by salicylic acid. However, the suppression effect of verapamil or LaCl3 on medium alkalinization induced by salicylic acid was higher than that of LiCl or 2-APB. When two types of calcium channel inhibitor (LaCl3 and 2-APB) were used together, the medium alkalinization induced by salicylic acid was completely suppressed and even reduced the pH in medium. On the other hand, A23187 could promote the medium alkalinization. Based on the results above, we speculated that salicylic acid could induce significant medium alkalinization in S. miltiorrhiza culture, depending on the calcium from both extracell and intracell. Moreover, calcium from extracell plays a more dominant role in this process. Reveal of relationship in this research between Ca2+ and medium alkalinization can provide theory evidence for mechanism of the plant secondary metabolism.
Calcimycin ; pharmacology ; Calcium ; chemistry ; Calcium Channel Blockers ; pharmacology ; Calcium Ionophores ; pharmacology ; Cell Culture Techniques ; Culture Media ; chemistry ; Salicylic Acid ; pharmacology ; Salvia miltiorrhiza ; metabolism ; Verapamil ; pharmacology

Objective To explore the expression levels of serum microRNA-1-3p(miR-1-3p)and activating transcription factor 4(ATF4)in elderly patients with chronic heart failure(CHF)and their relationships with cognitive impairment.Methods A total of 150 elderly patients with CHF were selected as the study subjects.Based on the Montreal Cognitive Assessment(MoCA)score,the patients were divided into cognitive impairment group(n=75)and normal cognitive function group(n=75).Real-time fluorescent quantitative polymerase chain reaction(RT-qPCR)was used to de-tect the serum miR-1-3p levels in both groups.Enzyme-linked immunosorbent assay(ELISA)was employed to measure the serum ATF4 levels in the two groups.Pearson correlation analysis was ap-plied to analyze the correlation between serum miR-1-3p and ATF4 level in elderly CHF patients with cognitive impairment.Logistic regression analysis was conducted to identify the influencing factors of cognitive impairment in elderly CHF patients.The predictive value of serum miR-1-3p and ATF4 levels for cognitive impairment in CHF patients was evaluated using receiver operating characteristic(ROC)curves.Results The left ventricular ejection fraction(LVEF),MoCA score and serum miR-1-3 p level were significantly lower in the cognitive impairment group,while the N-terminal pro-brain natriuretic peptide(NT-proBNP)level and serum ATF4 level were significantly higher than those in the normal cognitive function group(P＜0.05).There was a negative correlation between serum miR-1-3 p level and serum ATF4 level in elderly CHF patients with cognitive impairment(r=-0.523,P＜0.001).Both miR-1-3p and ATF4 were identified as influencing factors for cognitive impairment in elderly CHF patients(P＜0.05).The area under the curve(AUC)for predicting cognitive impairment in elderly CHF patients using a combination of serum miR-1-3p and ATF4 was significantly greater than the AUCs for prediction of serum miR-1-3p or ATF4 alone(P＜0.05 or P＜0.001).Conclusion The expression level of serum miR-1-3p is positively correlated while the expression of serum ATF4 is negatively correlated with cognitive function in elderly CHF patients.The serum miR-1-3 p and ATF4 may serve as potential markers for evaluating cognitive impairment in elderly CHF patients.

Objective To explore the expression levels of serum microRNA-1-3p(miR-1-3p)and activating transcription factor 4(ATF4)in elderly patients with chronic heart failure(CHF)and their relationships with cognitive impairment.Methods A total of 150 elderly patients with CHF were selected as the study subjects.Based on the Montreal Cognitive Assessment(MoCA)score,the patients were divided into cognitive impairment group(n=75)and normal cognitive function group(n=75).Real-time fluorescent quantitative polymerase chain reaction(RT-qPCR)was used to de-tect the serum miR-1-3p levels in both groups.Enzyme-linked immunosorbent assay(ELISA)was employed to measure the serum ATF4 levels in the two groups.Pearson correlation analysis was ap-plied to analyze the correlation between serum miR-1-3p and ATF4 level in elderly CHF patients with cognitive impairment.Logistic regression analysis was conducted to identify the influencing factors of cognitive impairment in elderly CHF patients.The predictive value of serum miR-1-3p and ATF4 levels for cognitive impairment in CHF patients was evaluated using receiver operating characteristic(ROC)curves.Results The left ventricular ejection fraction(LVEF),MoCA score and serum miR-1-3 p level were significantly lower in the cognitive impairment group,while the N-terminal pro-brain natriuretic peptide(NT-proBNP)level and serum ATF4 level were significantly higher than those in the normal cognitive function group(P＜0.05).There was a negative correlation between serum miR-1-3 p level and serum ATF4 level in elderly CHF patients with cognitive impairment(r=-0.523,P＜0.001).Both miR-1-3p and ATF4 were identified as influencing factors for cognitive impairment in elderly CHF patients(P＜0.05).The area under the curve(AUC)for predicting cognitive impairment in elderly CHF patients using a combination of serum miR-1-3p and ATF4 was significantly greater than the AUCs for prediction of serum miR-1-3p or ATF4 alone(P＜0.05 or P＜0.001).Conclusion The expression level of serum miR-1-3p is positively correlated while the expression of serum ATF4 is negatively correlated with cognitive function in elderly CHF patients.The serum miR-1-3 p and ATF4 may serve as potential markers for evaluating cognitive impairment in elderly CHF patients.