Objective The prevalence rate of type 2 diabetes mellitus in schizophrenia patients were significantly higher than normal people.The study examined the glucose metabolism in first-episode, drug-naive patients with schizophrenia. Methods Case-control study was employed.According to the 4th edition of American Diagnostic and Statistical Manual of Mental Disorders, 58 first-episode, drug-naive patients with schizophrenia hospitalized in our hospital were collected for the study.Brief psychiatric rating scale, Hamilton depressive scale and assessment of abnormal involuntary movement were used to assess the mental state and the degree of illness.Meanwhile, 60 hospitalized Han patients in the Second Hospital of Lanzhou University were selected as the control group. Fast plasma glucose (FPG) were detected in the morning on each patient along with oral glucose tolerance test (OGTT).Measure-ments were also made on height, body weight, waist circumference, hip circumference, as well as WHR and body mass index (BMI). Results No significant difference was found in gender, age, diet habit, activity, BMI and the number of education years between the groups(P>0.05).The average FPG of the patient group was higher than that of the control group(5.29 ±0.83 mmol/L vs 4.37 ±0.54 mmol/L);postprandial 2 hour glucose of the patient group was significantly higher than that of the control group ( 6.89 ±0.98 ) mmol/L vs 5.97 ±0.82 mmol/L, P<0.05).Statistical difference in the incidence of impaired glucose tolerance( IGT) was found between the two groups, 8 paitents in patient group(13.8%), 2 in control group(3.3%) (χ2 =4.25,P<0.05).Patients with normal and ab-normal glucose tolerance showed no difference in mental state and illness degree(P>0.05). Conclusions First-episode, drug-na-ive patients with schizophrenia have more impaired fasting glucose tolerance than normal people.In order to identify and intervene the abnormal glucose metabolism of schizophrenia patients, it is of great importance to measure relation index to glucose metabolism, espe-cially the oral glucose tolerance test.

Objective To characterize the audiological features in the infants with otitis media with effusion(OME)and to investigate the utility of variety of objective andiometry methods in diagnosis and intervention on OME.Methods Fifry six infants(40 males and 16 females)were investigated,who were referred to our clinic at the General Hospital of Chinese People's Liberation Army by the other hospitals from December 2004 to June 2007 when the infants were diagnosed or highly suspected of OME.The ages at the initial diagnosis ranged from 42 days to three years,with an average of five months.The infants,after receiving the conventional otolaryngological exams,were subjected to the tests of auditory brainstem response (ABR),otoacoustic emission(OAE),tympanometry(226 Hz and 1000 Hz)and behaviors audiometry.Results Among 56 affected infants.87 ears were diagnosed with OME,of which 31 infants were affected bilateral and 25 with monaural.For the 49 infants who received hearing screening at birth.36 infants were referred at the initial screening.For the 52 infants who received repeated screening.all subjects were referred.Six infants without receiving hearing screening came to clinic when their parents observed their kids'hearing impairment.Among the 52 cases(104 ears)who received tympanometry test,20 subjects (28 ears)showed B or C type tympanometry curve.Thirty-nine cases(78 ears)were given tympanometry test at 1000 Hz,of which 38 cases(55 ears)showed abnormal hearing.Among 56 infants(112 ears)with ABR test,49 subjects(74 ears)exhibited prolonged ABR type Ⅰ curve.All 56 infants(112 ears)received OAE test,of which 55 subjects(81 ears)were referred Four infants(8 ears)accepted the behavior test and all of them showed A-B Gap.Conclusions The combined tympanometry test at both 226 Hz and 1000 Hz,ABR latency or threshold test,infant's behavior test and OAE,used jointly,enable characterizing better OME in infants.thus helping early diagnosis of this hearing disorder.

Objective: To summarize the different cardiac shunt presentation in right heart contrast echocardiography and to clarify its clinical value for diagnosing the patients with congenital heart disease (CHD).
Methods: We retrospectively analyzed 102 patients who received right heart contrast echocardiography in our hospital from 2006 to 2014. The contrast media was the hand-vibrated mixture of 9 ml 50% glucose solution with 1 ml air.
Results: There were 49/102 patients with abnormal blood shunt detected including 8 patients of atrial septal defect (ASD) with right to left or dual shunt, 7 of ASD with left to right shunt, 9 of patent foramen ovale (PFO) with functional right to left shunt, 13 of patent ductus arteriosus (PDA) combining pulmonary arterial hypertension, 3 of pulmonary arteriovenous ifstula (PAVF), 9 patients with persistent left superior vena cava (PLSVC) and 8 of them drain to coronary vein sinus, 1 drain to left atrium.
Conclusion: Right heart contrast echocardiography may simply and effectively detect abnormal blood shunt in patients combining pulmonary arterial hypertension and improve the diagnostic accuracy of CHD.

Catalytic activity of activated carbon supported tungstosilicic acidin synthesizing 2-methyl-2-ethoxycarbonylmethyl1,3-dioxolane, 2,4-dimethyl-2-ethoxycarbonylmethyl-l,3-dioxolane, cyclohexanone ethylene ketal, cyclohexanone 1,2-propanediol ketal, butanone ethylene ketal, butanone 1,2-propanediol ketal, 2-phenyl-1,3-dioxolane, 4-methyl-2-phenyl-1,3-dioxolane,2-propyl-1,3-dioxolane, 4-methyl-2-propyl-1,3-dioxolane was reported. It has been demonstrated that activated carbon supported tungstosilicic acid is an excellent catalyst. Various factors involved in these reactions were investigated. The optimum conditions found were: molar ratio of aldehyde/ketone to glycol is 1/1.5, mass ratio of the catalyst used to the reactants is 1.0%, and reaction time is 1.0 h. Under these conditions, the yield of 2-methyl-2-ethoxycarbonylmethyl-l,3-dioxolane is 61.5%, of 2,4-dimethyl2-ethoxycarbonylmethyl-1,3-dioxolane is 69.1%, of cyclohexanone ethylene ketal is 74.6%, of cyclohexanone 1,2-propanediol ketal is 80.1%, of butanone ethylene ketal is 69.5%, of butanone 1,2-propanediol ketal is 78.5%, of 2-phenyl-1,3-dioxolane is 56.7%, of 4-methyl-2-phenyl- 1,3-dioxolane is 86.2%, of 2-propyl-1,3-dioxolane is 87.5%, of 4-methyl-2-propyl-1,3-dioxolane is 87.9%.

Objective To study the serum HBV cccDNA and genotype of hepatitis B virus in children in Gansu province.Methods 124 HBV-DNA positive children were randomly selected,with 84 males and 40 females.Among the 124 patients,65 were HBV carriers,59 were chronic hepatitis(31 mild,18 moderate and 10 severe).Genotypes of their serum HBV,liver function,HBV-DNA load and serum HBV cccDNA were detected.Results In the moderate and severe groups,HBV cccDNA positive rate was higher than that in the HBV carriers or the mild group(F=25.429,P＜0.01).The HBV cccDNA detection rate in HBeAg positive group was higher than that in the HBeAg negative group(F=28.386,P＜0.01).In the HBV cccDNA positive group,glutamic-pyruvic transaminase,glutamic-oxaloacetic transaminase,total bilirubin were higher than that in the negative group(t respectively 13.241,11.347,15.013,P＜0.01).Both C and B genotypes appeared to be the majority while C genotype was dominant in the 124 cases of children hepatitis,with the rest as B/C and some other genotypes.The positive rate of HBV cccDNA C genotype was higher than that of the genotype B(F=23.216,P＜0.01)and the negative rate of HBV cccDNA genotype was higher than that of the C genotype(F=26.364,P＜0.01).Conclusion Higher detection rate was found inthose more severe cases in the peripheral blood streams.HBV cccDNA and genotype testing might better reflect the level of HBV replication and the clinical severity of the disease,showing its guiding role in clinical diagnosis and treatment of hepatitis B.

ObjectiveTo explore the possibility and significance that psychotic patients treated in open wards in general hospital,a new managing model for psychosis cases.Methods1 200 psychotic cases were treated in the open ward,that was a new managing model for psychotic patients.ResultsNew managing model had following advantages: a. free for patients to communicate with outsides and keep their secret and human rights; b. having better effective,shorter housing days,and more economy compared with controlled cases.ConclusionSetting up open managing psychosis wards in general hospital can help treatment of psychotic patients,and it is the tendency of modern mental health.

OBJECTIVETo prepare monoclonal antibodies (mAbs) against enoyl-CoA hydratase 1 (ECH1).

METHODSNormal human liver tissues were homogenized, and the mitochondria were isolated by differential centrifugation. The total mitochondrial proteins were used to immunize BALB/c mice to prepare mAbs by routine hybridoma technique. The mAbs were characterized by ELISA, Western blotting and immunohistochemistry. The specificity of the antibody was identified by mass spectrometry (MS) following immunoprecipitation (IP) and confirmed by Uni-ZAP expression library screening.

RESULTSOne clone of the hybridoma BGB095 secreting specific mAb against ECH1 was obtained. The mAb was identified to belong to Ig subclass IgG1 and could be used in ELISA, Western blotting, immunohistochemistry, and immunoprecipitation.

CONCLUSIONA hybridoma cell line stably secreting specific mAb against ECH1 has been established. The specific mAb against ECH1 can be of great value for functional and distribution studies of ECH1.

Animals ; Antibodies, Monoclonal ; analysis ; immunology ; Antibody Specificity ; Blotting, Western ; Cell Line ; Enoyl-CoA Hydratase ; immunology ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunohistochemistry ; Liver ; cytology ; metabolism ; Mice ; Mice, Inbred BALB C ; Mitochondria ; metabolism

Objective To explore the effect of nursing intervention on quality of life and adverse effects of nasopharyngeal carcinoma patients with radiotherapy. Methods A total of 52 patients were randomly divided into two groups. The control group (26patients) received routine nursing, while the experimental group (26 patients) received nursing intervention by nurses. The effect were evaluated from two aspects: the quality of life and the rate of adverse effect. Results The quality of life in the experimental group was higher than that in the control group, while adverse effects were on the contrary. Conclusions Nursing intervention as an effective nursing model can improve the quality of life and decrease adverse effects of patients with nasopharyngeal carcinoma.

OBJECTIVETo investigate the relationship between genotype of hepatitis B virus and hepatitis B virus related-glomerular nephritis in (HBV-GN) children.

METHODTotally 176 HBV-DNA positive children with chronic hepatitis B were randomly collected. Among the 176 patients, 92 were HBV carriers, 84 were cases with chronic hepatitis. The genotypes of their serum HBV, liver function, and HBV-DNA load were detected. When children showed nephrotic syndrome, renal biopsy was performed.

RESULTOf the serum samples of 176 cases, 85 (48.3%) were genotype C, 72 (40.9%) were genotype B, 13 (7.4%) were genotype B/C, and 6 (3.4%) were non-B/C genotype which were excluded. Among the analyzed 157 cases, the ratio of HBV-GN in the HBeAg positive group (78.3%) was significantly higher than that in the negative group (21.7%) (χ(2) = 18.301, P < 0.001). And, the ratio of HBV-GN in the genotype C group (73.9%) was significantly higher than that in the genotype B group (26.1%) (P < 0.039). The ratio of hematuria or proteinuria in the genotype C group (20%, 18.8%) was significantly higher than that in the genotype B group (8.3%, 5.6%) (P < 0.039; P value = 0.013); and the alteration of ALT or C3 in the genotype C group (10.2%, 15.3%) was more frequent than those in the genotype B group (2.8%, 2.8%) (P = 0.005; P = 0.008). There were no significant differences in kidney dysfunction or hepatomegaly. Further, the ratio of HBV-GN was more significantly frequent in HBV-DNA highly loading group (79.2%) than which in HBV-DNA lowly loading group (20.8%) (P = 0.000). Finally, in HBV-GN group, genotype C cases (88.2%) more frequently had high HBV-DNA load condition than genotype B cases (11.8%) (P = 0.021).

CONCLUSIONChildren with HBV infection in Gansu province showed mainly genotypes C or B, while genotype C seemingly predominant. Patients with genotype C more frequently showed proteinuria or hematuria. The high HBV-DNA load may be related with HBV-GN. It is a potential reason in the mechanism of HBV-GN that patients with genotype C had more possibility to have HBV-DNA high load. Analysis of HBV genotype for HBV patients maybe helpful in diagnosis and treatment.

Adolescent ; Biopsy, Needle ; Child ; Child, Preschool ; China ; epidemiology ; DNA, Viral ; blood ; genetics ; Female ; Genotype ; Hepatitis B ; blood ; epidemiology ; virology ; Hepatitis B virus ; genetics ; Humans ; Infant ; Male ; Nephritis ; epidemiology ; pathology ; virology ; Viral Load

Catalytic activity of activated carbon supported tungstosilicic acid in synthesizing 2-methyl-2-ethoxycarbonylmethyl- 1,3-dioxolane, 2,4-dimethyl-2-ethoxycarbonylmethyl-1,3-dioxolane, cyclohexanone ethylene ketal, cyclohexanone 1,2-propa- nediol ketal, butanone ethylene ketal, butanone 1,2-propanediol ketal, 2-phenyl-1,3-dioxolane, 4-methyl-2-phenyl-1,3-dioxolane, 2-propyl-1,3-dioxolane, 4-methyl-2-propyl-1,3-dioxolane was reported. It has been demonstrated that activated carbon supported tungstosilicic acid is an excellent catalyst. Various factors involved in these reactions were investigated. The optimum conditions found were: molar ratio of aldehyde/ketone to glycol is 1/1.5, mass ratio of the catalyst used to the reactants is 1.0%, and reaction time is 1.0 h. Under these conditions, the yield of 2-methyl-2-ethoxycarbonylmethyl-1,3-dioxolane is 61.5%, of 2,4-dimethyl- 2-ethoxycarbonylmethyl-1,3-dioxolane is 69.1%, of cyclohexanone ethylene ketal is 74.6%, of cyclohexanone 1,2-propanediol ketal is 80.1%, of butanone ethylene ketal is 69.5%, of butanone 1,2-propanediol ketal is 78.5%, of 2-phenyl-1,3-dioxolane is 56.7%, of 4-methyl-2-phenyl-1,3-dioxolane is 86.2%, of 2-propyl-1,3-dioxolane is 87.5%, of 4-methyl-2-propyl-1,3-dioxolane is 87.9%.