Adolescent ; Heart Valve Diseases ; etiology ; Heart Valves ; pathology ; Humans ; Lupus Erythematosus, Systemic ; complications ; pathology ; Male

?AIM: To study the changes of choroidal thickness and hemodynamic parameters in patients with diabetic retinopathy and their influencing factors.?METHODS: From January 2013 to January 2015, 100 patients (100 eyes) with type 2 diabetes were divided into 3 groups:34 patients without diabetic retinopathy ( NDR) , 36 patients with non proliferative diabetic retinopathy ( NPDR) group, and 30 patients with proliferative diabetic retinopathy (PDR). According to the results of OCT, the patients with diabetic retinopathy were divided into 2 groups: diabetic macular edema ( DME ) group ( 28 cases) , and 38 cases without diabetic macular edema. During the same period in our hospital 35 subjects for physical examination were selected as the control group. The hemodynamic parameters of the posterior ciliary artery in different groups of patients at different distance from the center of the macular were compared, analyzing influencing factors.?RESULTS:With the aggravation of diabetic retinopathy, the choroidal thickness in different distance from the center of the macular decreased. The choroidal thickness of NPDR and PDR group were thinner than that of the control group (P<0. 05). The choroidal thickness of NDR group was not different from the control group (P>0. 05). There was no significant difference in the choroidal thickness between the DME patients and non DME patients (P>0. 05). Pearson correlation analysis showed that there was no significant correlation between choroidal thickness of patients with diabetic retinopathy and diabetic duration, fasting blood glucose, HbA1c, eye axis length, systolic blood pressure and diastolic blood pressure (P>0. 05), but there was a correlation with BCVA (logMAR) (P<0. 01). EDV and PSV in NDR group and NPDR group were significantly lower than those in control group, RI was higher than that in control group. PSV and EDV in PDR group were significantly lower than those in other three groups, RI was higher than the other three groups, and the difference was statistically significant ( P<0. 05).?CONCLUSION:With the severity of retinopathy in type 2 diabetic patients, the choroidal thickness decreased, and the thickness of the choroid is beneficial in the comprehensive analysis of 2 diabetic retinopathy.

To investigate the induction of apoptosis of mouse colonic adenocarcinoma CT26 cells by recombinant Clostridium difficile toxin B (rTcdB), CT26 cells were exposed to different concentrations of rTcd B. Inhibition of cell proliferation was measured by MTT assay. The activation of Caspase 3 was measured by colorimetric method. Cell morphological analysis and flow cytometry were performed to confirm cell apoptosis. rTcd B inhibited the proliferation of CT26 cells in a timeand dose-dependent manner. Caspase 3 activity in CT26 cells was elevated remarkably after rTcd B exposure for 6 h, 12 h, 18 h or 24 h, as compared with the control group. Morphological changes were observed by fluorescence microscopy. The exposure of rTcd B to CT26 cells induced a timeand dose-dependent apoptotic cell death as determined by flow cytometry analysis. The results showed that recombinant Clostridium difficile toxin B induced apoptosis of CT26 cells.

There have been many studies on the nutrition and the growth status of children from rural and remote western regions of China,whereas researches on children from urban low-income families are scarce.This study aimed to investigate the growth and nutritional status of children under five years of age from urban low-income families in China.There were 169 children aged 25-60 months recruited from Xiangtan and Jilin,two cities with a population of 2.81 million and 4.26 million respectively,in China in this cluster cross-sectional study.Data were collected on demographic and socioeconomic characteristics,the feeding practices and the incidence of anemia and diarrhea.The results showed that the prevalence of low birth weight and macrosomia was 7.l％ and 9.5％ for the two cities,respectively,which was higher than that for other cities in China (1.5％ and 5.9％).Of all the sampled children,14.6％ and 8.2％ suffered anemia and diarrhea,respectively.Multivariate analysis showed that legumes or nuts fed in a 24-h recall increased the risk of anemia (OR=4.9).Children whose caregivers began to introduce complementary foods relatively late would have high diarrhea prevalence (OR=1.4).In conclusion,the prevalence of anemia and diarrhea in under-five children from urban low-income families in China is relatively high.The growth and nutritional status of these children is greatly affected by feeding practices.A series of measures should be taken by relevant government departments to improve the health of these children.

OBJECTIVETo prepare monoclonal antibodies (mAbs) against enoyl-CoA hydratase 1 (ECH1).

METHODSNormal human liver tissues were homogenized, and the mitochondria were isolated by differential centrifugation. The total mitochondrial proteins were used to immunize BALB/c mice to prepare mAbs by routine hybridoma technique. The mAbs were characterized by ELISA, Western blotting and immunohistochemistry. The specificity of the antibody was identified by mass spectrometry (MS) following immunoprecipitation (IP) and confirmed by Uni-ZAP expression library screening.

RESULTSOne clone of the hybridoma BGB095 secreting specific mAb against ECH1 was obtained. The mAb was identified to belong to Ig subclass IgG1 and could be used in ELISA, Western blotting, immunohistochemistry, and immunoprecipitation.

CONCLUSIONA hybridoma cell line stably secreting specific mAb against ECH1 has been established. The specific mAb against ECH1 can be of great value for functional and distribution studies of ECH1.

Animals ; Antibodies, Monoclonal ; analysis ; immunology ; Antibody Specificity ; Blotting, Western ; Cell Line ; Enoyl-CoA Hydratase ; immunology ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunohistochemistry ; Liver ; cytology ; metabolism ; Mice ; Mice, Inbred BALB C ; Mitochondria ; metabolism

The influenza A virus matrix protein2 gene(M2)which deleted transmembrane region was amplified by overlap extending PCR,and the multiepitope gene of hemagglutinin(HA)was PCR amplified with seven continuous synthesized segments by designing primer.The two gene segments were separately cloned into pMD18T vector to sequence analysis and prokarytic expression vector pET28a+ to construct the recombinant plasmid pET28a+M2dHA.The recombinant plasmid was transformed into E.coli BL21(DE3),and the high expression strain was obtained by screening monoclones.The recombinant protein existed as inclusion bodies,which accounted about 45% of the total cellular protein.The inclusion bodies were washed with 1% Triton X100 solution twice,and dissolved in 8 mol/L urea solution.The solution protein was purified by Ni+2 affinity chromatography,and refolded by dilution renaturation,then purified by Q Sepharose FF cation exchange column.The purity of the protein was over 90% by HPLC analysis.The result of Western blot showed it has good antigenicity and specificity.These results strongly supported for the further study of the broadspectrum influenza virus subunit vaccine.

Objective To evaluate the application value of the new chinese diabetes risk score in the clinical diagnosis of type 2 diabetes mellitus.Methods A total of 232 subjects who received physical examination at the outpatient department of endocrinology were selected.Medical history and demographic information were collected.Physical examination and 75 g oral glucose tolerance test (OGTT)were conducted.Fasting or 2 -h blood glucose,HbA1 c,serum triglyceride (TG), total cholesterol (TC)and low density lipoprotein cholesterol (LDL-C)were measured.Results The area under the receiver operating curve was 0. 788 (95%CI=0. 725 -0. 852),with 0. 832 (95%CI=0. 748 -0. 916)in males and 0. 754 (95%CI=0. 664-0. 844)in females.At the optimal cutoff value (25 scores)for detecting type 2 diabetes,the sensitivity was 88. 06%and the specificity was 37. 58%.The Diabetes Risk Score was positively related to the probability of type 2 diabetes.Conclusion The new chinese diabetes risk score could be a reliable screening tool to evaluate undiagnosed type 2 diabetes in the Chinese population.

OBJECTIVETo study on visible display of Meridian on the dummy human body.

METHODSTube model-building method and computer technique were used, and data came from Voxel-Man dummy human body development platform.

RESULTSThe visual effect of re-building Meridian is very good and it can display the different layers of anatomic structures on the Meridian lines.

CONCLUSIONThe visible display of Meridian on the dummy human body is preliminary realized, which provides data carriers for establishing the platform of Meridian study.

Human Body ; Humans ; Meridians

OBJECTIVETo study the status of infection and type-specific distribution on human papillomavirus (HPV) in the urban areas of Shenyang city, China and to provide data for vaccine introduction.

METHODSA list of 1000 women aged 15-59 years were obtained randomly from the local population, and a questionnairey was filled in. Samples of cervical cell were obtained from 702 women who had had sexual experience and were not pregnant during the study was carried on. HPV type was detected using a GP5+/6+ -based PCR, liquid-based cytology, VIA, VILI and electronic coloscopy were used for screening on cervical cancer.

RESULTSThe prevalence of any HPV type was 17.0% among the 702 women who had been enrolled in this study, 64.1% in women with abnormal cytology, 11.1% in women with normal cytology and the difference was significant. The prevalence of high-risk HPV type was 11.9% and the low-risk type was 6.5%. The prevalence of either high-risk or low-risk HPV type was significantly higher in women with abnormal cytology than those with normal cytology. There were a total number of 32 HPV types identified and the most common HPV types were 16 (3.4%), 52 (2.4%) and 58 (1.9%). The prevalence rates of either high-risk or low-risk HPV type were not statistically different among different age-groups with P>0.05. HPV types with high risk were predominantly distributed in the groups below 45 years old and the difference was significant with P < 0.05. There was no significant difference between high-risk and low-risk types above 45 years old.

CONCLUSIONThe prevalence of HPV in women with abnormal cytology was statistically higher than those with normal cytology. The most common HPV types in this population were 16, 52 and 58, and the validity of present vaccines was not good enough for cervical cancer prevention in this local area.

Adolescent ; Adult ; China ; epidemiology ; Female ; Humans ; Middle Aged ; Papillomaviridae ; classification ; isolation & purification ; Papillomavirus Infections ; epidemiology ; Population Surveillance ; Prevalence ; Risk Factors ; Urban Population ; Young Adult

AIMTo study the effect of uvarigrin on mitochondrial dependent pathway during the apoptosis induced by it in MDR KBv200 cells and their parental sensitive KB cells.

METHODSMTT assay was used to detect the cytotoxic effect of uvarigrin on KBv200 and KB cells. Annexin V FITC staining identified uvarigrin-induced apoptosis in KBv200 and KB cells. These cells underwent incubation with DCFH-DA, or DiOC6, followed by flowcytometry for the measurement of reactive oxygen species (ROS) and mitochondrial membrane potential (deltapsim), respectively. The Western blotting analysis was performed on Caspase-9 activation.

RESULTSUvarigrin inhibited the growth of KBv200 cells and KB cells in vitro. Most of the uvarigrin-induced cells death was found to be due to apoptosis, as determined by Annexin V FITC staining. During the apoptosis, the level of ROS increased while the level of deltapsim decreased in a time-dependent manner. Uvarigrin triggered Caspase-9 activation.

CONCLUSIONUvarigrin induced apoptosis in KBv200 cells and KB cells probably through a mitochondria-dependent pathway.

Antineoplastic Agents, Phytogenic ; administration & dosage ; isolation & purification ; pharmacology ; Apoptosis ; drug effects ; Caspase 9 ; metabolism ; Cell Proliferation ; drug effects ; Dose-Response Relationship, Drug ; Drug Resistance, Multiple ; Drug Resistance, Neoplasm ; Furans ; administration & dosage ; isolation & purification ; pharmacology ; Humans ; KB Cells ; Lactones ; administration & dosage ; isolation & purification ; pharmacology ; Membrane Potentials ; drug effects ; Mitochondria ; physiology ; Plants, Medicinal ; chemistry ; Reactive Oxygen Species ; metabolism ; Uvaria ; chemistry