Actins ; metabolism ; Adult ; Antigens, Neoplasm ; metabolism ; Diagnosis, Differential ; Epithelioid Cells ; chemistry ; pathology ; Female ; Humans ; Hysterectomy ; Immunohistochemistry ; Melanoma-Specific Antigens ; Mesenchymoma ; metabolism ; pathology ; surgery ; Neoplasm Proteins ; metabolism ; Uterine Neoplasms ; metabolism ; pathology ; surgery

Adult ; Diagnosis, Differential ; Female ; Humans ; Hysterectomy ; Leiomyomatosis ; pathology ; surgery ; Sarcoma, Endometrial Stromal ; pathology ; Uterine Neoplasms ; pathology ; surgery ; Uterus ; blood supply ; Vascular Neoplasms ; pathology ; surgery

Adult ; Diagnosis, Differential ; Heart Neoplasms ; metabolism ; pathology ; surgery ; Humans ; Immunohistochemistry ; Liposarcoma, Myxoid ; metabolism ; pathology ; surgery ; Male ; Myxoma ; metabolism ; pathology ; Myxosarcoma ; metabolism ; pathology ; Pericardium ; S100 Proteins ; metabolism ; Vimentin ; metabolism

In order to confirm the role that the 49th amino acid residue plays in enzymatic inactivity of Glutamine 49 phospholipase A2(Gln49-PLA2),site-directed mutagenesis of its 49th amino acid gene codon was conducted using PCR.Aspartic acid 49 phospholipase A2(Asp49-PLA2-Q49D-PLA2),the mutant of Gln49-PLA2 was expressed in E.coli with pET32a+ vector.The fusion protein,expressed as inclusion body,after being denatured,was on-column refolded and purified by immobilized metal affinity chromatography(IMAC),and then cleaved by Factor Xa.The mature Q49D-PLA2 mutant was obtained by Hitrap SP cation exchange and Superdex 75 gel filtration chromatography,with the recovery rate of 1.3%,and the specific activity of the mature Q49D-PLA2 mutant was 72 U/mg.It has been demonstrated that the 49th glutamine amino acid residue is the main reason in enzymatic inactivity of Gln49-PLA2 and the results are helpful for denatured protein refolding,especially in rich disulfide bonds conditions.

Objective To evaluate effects of incorporating tetrapod-like zinc oxide whisker (T-ZnOw) antibacterial agent on the antibacterial activity of composite resin, compared with that of the silver-based inorganic antibacterial agent. Methods The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the two different antibacterial agents against Streptococcus mutans were investigated using the broth dilution test Then the antibacterial activities of the self-cured composite resin specimens with different incorporating concentrations of the two antibacterial agents were evaluated using direct contact test And the antibacterial activities of the resin specimens were examined again after 3 months of accelerating aging. Results The MIC (MBC) of the T-ZnOw antibacterial agent and silver-based inorganic antibacterial agent were 0.15625(0.3125) g/L and 0. 15625(0.15625) g/L When the concentrations of T-ZnOw added to composite resin were 3%, 5% , and 10% (w/w), the antibacterialratios of the resin specimens were (84.85±5.16)%, (94.22 ± 3.73)%, and (99.43±0.48)%respectively. When the concentrations of the silver-based antibacterial agent added to composite resin were 1%, 3%, and 5%, the antibacterial ratios were (71.01±7.67)%, (90.76±5.91)%, and (97. 19 ±4.10)% respectively. The antibacterial ratios of the resin specimens containing 5% of both antibacterial agents were (89.89 ± 5.55) % and (78.79 ± 7.81) % respectively after 3 months of accelerating aging.Conclusions Incorporation of the T-ZnOw antibacterial agent in composite resin can improve the antibacterial performance of the resin, and the resin exhibits better antibacterial performance than that incorporating silver-based inorganic antibacterial agent after 3 months of aging.

OBJECTIVETo investigate the diagnosis and differential diagnosis of extranodal Rosai-Dorfman disease.

METHODSTwo cases of extranodal Rosai-Dorfman disease were studied using hematoxylin-eosin, and immunohistochemical staining, along with a literature review.

RESULTSThe lesions of RDD were characterized by the presence of large histiocytes with emperipolesis, accompanied by infiltration of lymphocytes, plasma cells and other inflammatory cells. The large histiocytes had an abundant cytoplasm, pale to eosinophilic in appearance, positive for S-100 protein staining, with a vesicular nucleus and a small basophilic nucleolus in each cell.

CONCLUSIONSExtranodal Rosai-Dorfman disease is known as an idiopathic proliferative disease of histiocytes with a distinct morphologic feature and is very rare. Differential diagnosis from other types of fibrohistiocytic proliferation lesions is recommended.

Antigens, CD ; metabolism ; Antigens, Differentiation, Myelomonocytic ; metabolism ; Brain ; pathology ; surgery ; Brain Diseases ; metabolism ; pathology ; surgery ; Dermatologic Surgical Procedures ; Diagnosis, Differential ; Histiocytosis, Sinus ; metabolism ; pathology ; surgery ; Humans ; Male ; Middle Aged ; S100 Proteins ; metabolism ; Skin ; pathology ; Skin Diseases ; metabolism ; pathology ; surgery

OBJECTIVETo determine the effect of early enteral nutrition (EN) on immune function of the patients after operation for severe abdominal trauma.

METHODSFourty patients who underwent operation for severe abdominal trauma were randomly divided into two groups, and received an early enteral nutrition (EN group, n=20) through jejunal nutritional tube from postoperative day 1, or parental nutrition (PN group, n=20) for 7 days. C3, IgA, IgM, IgG and CD3+, CD4+, CD8+, CD4+/CD8+ of the two groups patients were detected on the day before operation and the postoperative day 1 and 8. The infection complications were compared.

RESULTSAfter 7 days, the levels of C3+, IgA, IgG, CD3+, CD4+, CD8+, and CD4+/CD8+ in EN group increased significantly compared with those in PN group (P< 0.05). The incidence of infection was 10% in EN group, while 30% in PN group (P< 0.05).

CONCLUSIONEarly enteral nutrition can improve immune function and decrease postoperative infection after operation for severe abdominal trauma.

Abdominal Injuries ; immunology ; surgery ; Adolescent ; Adult ; CD3 Complex ; analysis ; CD4 Antigens ; analysis ; CD4-CD8 Ratio ; CD8 Antigens ; analysis ; Complement C3 ; analysis ; Enteral Nutrition ; Female ; Humans ; Immunoglobulin A ; analysis ; Immunoglobulin G ; analysis ; Male ; Postoperative Period ; Young Adult

OBJECTIVEClinical pathways are standardized, multidisciplinary, integrated management plans. This study aimed to evaluate the efficacy of clinical pathways in children with Rotavirus enteritis.

METHODSSeventy-one children with Rotavirus enteritis were treated according to the clinical pathways. Seventy-five children with Rotavirus enteritis who received routine therapy and nursing interventions served as the control group. The clinical efficacies were compared between the two groups.

RESULTSThe average hospitalization duration was shortened, the hospitalization costs were reduced and the parents' satisfaction rate increased in the observed group compared with the control group (P<0.05).

CONCLUSIONSThe use of clinical pathways may decrease the hospitalization duration and costs and improve the quality of nursing care and the parents' satisfaction rate in children with Rotavirus enteritis.

Critical Pathways ; Enteritis ; therapy ; Female ; Humans ; Infant ; Length of Stay ; Male ; Patient Satisfaction ; Rotavirus Infections ; therapy

OBJECTIVETo investigate the effects of incorporating three different zinc oxide (ZnO) on the antibacterial activity of composite resin.

METHODSThe minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of nano-ZnO, tetrapod-like zinc oxide whiskers (T-ZnOw), micro-ZnO against Streptococcus mutans were examined by the broth dilution test. Then the three different ZnO were added to the powder of one kind of bicomponent self-cured composite resin at 5% respectively, and the antibacterial activities of the resin specimens were evaluated using the membrane covering method before and after 3-month accelerating aging.

RESULTSThe MIC values of the three different ZnO against Streptococcus mutans were 78.13, 312.50 and 1 250.00 microg/mL respectively and the MBC values were 156.25, 625.00, 1,250.00 microg/mL respectively. The antibacterial ratios of the resin specimens incorporating with 5% of the three different ZnO were (93.58+/-5.95)%, (89.42+/-4.11)% and (78.97+/-3.90)% respectively, while after 3-month accelerating aging those were (89.01+/-7.91)%, (84.63+/-4.72)% and (72.27+/-3.89)%.

CONCLUSIONThe three different ZnO could improve the antibacterial activity of the composite resin. The nano-ZnO exhibit the strongest antibacterial activity, while the micro-ZnO weakest. The T-ZnOw presents comparatively strong antibacterial activity although with smaller specific surface area.

Anti-Bacterial Agents ; Composite Resins ; Microbial Sensitivity Tests ; Streptococcus mutans ; Zinc Oxide

Objective To investigate the anti -inflammatory effect and mechanism of volatile of Ramulus Cinnamom ( VORC) on acute pneumonia mice.Methods Ninety ICR mice were randomly divided into 6 groups, normal group, model group, positive control group and low, middle, high dose experimental group (experimental -L,-M,-H group), each group with 15 mice, acute pneumonia model mice were established by intraperitoneal ( ip) injection of escherichia coli lipopolysac-charide (LPS), normal group ip the same amount of normal saline , experimental group immediately gavaged VORC at 0.03, 0.05, 0.10 mL· kg -1, positive control group given 5 mg· kg -1dexamethasone acetate , model group and nor-mal group given equal volume of normal saline , and continuously treated for 5 days.The levels of inflammatory cyto-kines in serum and the number of inflammatory cells in bronchoalceolar lavage fluid (BALF) were detected, morpholo-gy of lung tissue in mice was observed by hematoxylin -eosin (HE) staining, the expression of transcriptional activator protein 1 (AP-1) in lung tissue was detected by reverse transcriptase -polymerase chain reaction ( RT -PCR) . Results After treatment, the interleukin -6 (IL-6) content of normal group, model group, positive control group, experimental -L,-M,-H group after treatment were (87.49 ±7.69 ), ( 138.12 ±14.36 ), ( 109.15 ±4.58 ), (112.33 ±65.26),(108.22 ±55.38),(100.26 ±63.42)ng· L-1,respectively; the interleukin-1β(IL-1β) con-tent were (12.16 ±5.12),(59.48 ±10.35),(43.15 ±7.14),(50.12 ±6.33),(42.52 ±6.98),(26.15 ±5.34) ng· L-1,respectively; the tumor necrosis factor-α(TNF-α) content were (332.45 ±15.28),(455.15 ±27.25), (426.21 ±16.47),(432.15 ±15.66),(422.35 ±13.52),(402.15 ±12.05)ng· L-1,respectively.The number of BALF macrophages of normal group , model group, positive control group , experimental -L,-M,-H group after treatment were were ( 98.13 ± 10.28 ), ( 199.47 ± 24.35 ), ( 159.86 ± 16.47 ), ( 160.24 ± 15.48 ), (151.24 ±12.85 ),( 145.26 ±9.52 )×106/L, respectively; the number of neutrophils were ( 5.28 ±0.54 ), (61.45 ±5.16),(36.52 ±5.37 ),(42.15 ±6.33 ),(35.48 ±5.96 ),(23.58 ±6.25 )×106/L,respectively; the number of white blood cells were (105.48 ±8.69 ), ( 325.14 ±21.58 ), ( 224.13 ±16.24 ), ( 256.41 ±19.46 ), (233.15 ±15.46 ),(189.46 ±10.25 )×106/L,respectively.The levels of serum interleukin -6 (IL-6), interleukin-1β(IL-1β) and tumor necrosis factor -α(TNF -α) and macrophages, neutrophils and white blood cells in BALF of model group were significantly higher than those of the normal group , while experimental -L,-M,-H group and positive control group were markedly lower than those of the model group ( P＜0.01 ).The relative expression of c-jun mRNA in lung tissue of normal group, model group, positive control group, experimental-L,-M,-H group were 0.38 ±0.15 , 0.59 ±0.13, 0.41 ±0.15, 0.47 ±0.16, 0.46 ±0.12 , 0.39 ±0.15 respectively; the relative ex-pression of c-fos mRNA in lung tissue were 0.52 ±0.24 , 0.92 ±0.23 , 0.56 ±0.14 , 0.75 ±0.22 , 0.72 ±0.19 , 0.58 ±0.21 , respectively.The relative expression of c -jun and c-fos mRNA in lung tissue of model group were sig-nificantly higher than those of the normal group , while the relative expression of c -jun and c-fos mRNA in positive control group and experimental -L,-M,-H group were significantly lower than those in the model group ( P＜0.05 or P＜0.01).Conclusion VORC can reduce the inflammatory and pathological damage of lung tissue in acute pneu-monia mice, which may be achieved by inhibiting the activation of AP -1 signaling pathway.