Cardiomyopathies ; diagnosis ; Carnitine ; deficiency ; Humans ; Hyperammonemia ; diagnosis ; Infant, Newborn ; Male ; Muscular Diseases ; diagnosis

Objective To study the serum HBV cccDNA and genotype of hepatitis B virus in children in Gansu province.Methods 124 HBV-DNA positive children were randomly selected,with 84 males and 40 females.Among the 124 patients,65 were HBV carriers,59 were chronic hepatitis(31 mild,18 moderate and 10 severe).Genotypes of their serum HBV,liver function,HBV-DNA load and serum HBV cccDNA were detected.Results In the moderate and severe groups,HBV cccDNA positive rate was higher than that in the HBV carriers or the mild group(F=25.429,P＜0.01).The HBV cccDNA detection rate in HBeAg positive group was higher than that in the HBeAg negative group(F=28.386,P＜0.01).In the HBV cccDNA positive group,glutamic-pyruvic transaminase,glutamic-oxaloacetic transaminase,total bilirubin were higher than that in the negative group(t respectively 13.241,11.347,15.013,P＜0.01).Both C and B genotypes appeared to be the majority while C genotype was dominant in the 124 cases of children hepatitis,with the rest as B/C and some other genotypes.The positive rate of HBV cccDNA C genotype was higher than that of the genotype B(F=23.216,P＜0.01)and the negative rate of HBV cccDNA genotype was higher than that of the C genotype(F=26.364,P＜0.01).Conclusion Higher detection rate was found inthose more severe cases in the peripheral blood streams.HBV cccDNA and genotype testing might better reflect the level of HBV replication and the clinical severity of the disease,showing its guiding role in clinical diagnosis and treatment of hepatitis B.

CD4 Antigens ; blood ; CD4-CD8 Ratio ; CD8 Antigens ; blood ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Seizures, Febrile ; immunology

OBJECTIVETo prepare monoclonal antibodies (mAbs) against enoyl-CoA hydratase 1 (ECH1).

METHODSNormal human liver tissues were homogenized, and the mitochondria were isolated by differential centrifugation. The total mitochondrial proteins were used to immunize BALB/c mice to prepare mAbs by routine hybridoma technique. The mAbs were characterized by ELISA, Western blotting and immunohistochemistry. The specificity of the antibody was identified by mass spectrometry (MS) following immunoprecipitation (IP) and confirmed by Uni-ZAP expression library screening.

RESULTSOne clone of the hybridoma BGB095 secreting specific mAb against ECH1 was obtained. The mAb was identified to belong to Ig subclass IgG1 and could be used in ELISA, Western blotting, immunohistochemistry, and immunoprecipitation.

CONCLUSIONA hybridoma cell line stably secreting specific mAb against ECH1 has been established. The specific mAb against ECH1 can be of great value for functional and distribution studies of ECH1.

Animals ; Antibodies, Monoclonal ; analysis ; immunology ; Antibody Specificity ; Blotting, Western ; Cell Line ; Enoyl-CoA Hydratase ; immunology ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunohistochemistry ; Liver ; cytology ; metabolism ; Mice ; Mice, Inbred BALB C ; Mitochondria ; metabolism

OBJECTIVETo investigate the relationship between genotype of hepatitis B virus and hepatitis B virus related-glomerular nephritis in (HBV-GN) children.

METHODTotally 176 HBV-DNA positive children with chronic hepatitis B were randomly collected. Among the 176 patients, 92 were HBV carriers, 84 were cases with chronic hepatitis. The genotypes of their serum HBV, liver function, and HBV-DNA load were detected. When children showed nephrotic syndrome, renal biopsy was performed.

RESULTOf the serum samples of 176 cases, 85 (48.3%) were genotype C, 72 (40.9%) were genotype B, 13 (7.4%) were genotype B/C, and 6 (3.4%) were non-B/C genotype which were excluded. Among the analyzed 157 cases, the ratio of HBV-GN in the HBeAg positive group (78.3%) was significantly higher than that in the negative group (21.7%) (χ(2) = 18.301, P < 0.001). And, the ratio of HBV-GN in the genotype C group (73.9%) was significantly higher than that in the genotype B group (26.1%) (P < 0.039). The ratio of hematuria or proteinuria in the genotype C group (20%, 18.8%) was significantly higher than that in the genotype B group (8.3%, 5.6%) (P < 0.039; P value = 0.013); and the alteration of ALT or C3 in the genotype C group (10.2%, 15.3%) was more frequent than those in the genotype B group (2.8%, 2.8%) (P = 0.005; P = 0.008). There were no significant differences in kidney dysfunction or hepatomegaly. Further, the ratio of HBV-GN was more significantly frequent in HBV-DNA highly loading group (79.2%) than which in HBV-DNA lowly loading group (20.8%) (P = 0.000). Finally, in HBV-GN group, genotype C cases (88.2%) more frequently had high HBV-DNA load condition than genotype B cases (11.8%) (P = 0.021).

CONCLUSIONChildren with HBV infection in Gansu province showed mainly genotypes C or B, while genotype C seemingly predominant. Patients with genotype C more frequently showed proteinuria or hematuria. The high HBV-DNA load may be related with HBV-GN. It is a potential reason in the mechanism of HBV-GN that patients with genotype C had more possibility to have HBV-DNA high load. Analysis of HBV genotype for HBV patients maybe helpful in diagnosis and treatment.

Adolescent ; Biopsy, Needle ; Child ; Child, Preschool ; China ; epidemiology ; DNA, Viral ; blood ; genetics ; Female ; Genotype ; Hepatitis B ; blood ; epidemiology ; virology ; Hepatitis B virus ; genetics ; Humans ; Infant ; Male ; Nephritis ; epidemiology ; pathology ; virology ; Viral Load

Female ; Hepatitis, Viral, Human ; therapy ; Humans ; Liver, Artificial ; Male ; Plasma Exchange ; methods ; Treatment Outcome

OBJECTIVEClinical pathways are standardized, multidisciplinary, integrated management plans. This study aimed to evaluate the efficacy of clinical pathways in children with Rotavirus enteritis.

METHODSSeventy-one children with Rotavirus enteritis were treated according to the clinical pathways. Seventy-five children with Rotavirus enteritis who received routine therapy and nursing interventions served as the control group. The clinical efficacies were compared between the two groups.

RESULTSThe average hospitalization duration was shortened, the hospitalization costs were reduced and the parents' satisfaction rate increased in the observed group compared with the control group (P<0.05).

CONCLUSIONSThe use of clinical pathways may decrease the hospitalization duration and costs and improve the quality of nursing care and the parents' satisfaction rate in children with Rotavirus enteritis.

Critical Pathways ; Enteritis ; therapy ; Female ; Humans ; Infant ; Length of Stay ; Male ; Patient Satisfaction ; Rotavirus Infections ; therapy

Objective To discuss the effect of phased reward behavior therapy on rehabilitation of patients with chronic schizophrenia.Methods 60 patients were randomly divided into treatment group (30 cases) and control group (30 cases) according to admission time.The treatment group received reward behavior therapy on the basis of drug treatment and traditional nursing care,while the control group received conventional work entertainment treatment activities.Brief Psychiatric Rating Scale (BPRS),Scale for Schizophrenia Positive Symptoms (SAPS),Negative Symptoms Scale (SANS) and the efficacy of inpatient rehabilitation of mental patients rating scale (IPROS)were used to evaluate the deficiency level of social function,degree of remission and rehabilitation of the patients before and 6 months after the treatment.Results The total average score of BPRS and IPROS of the treatment group were lower than the control group and the differences were significant [(8.81 ±6.41) vs (11.10±8.5),(19.15 ±14.11 ) vs (31.14 ± 16.10 ) ;t =3.18,7.61 ;P ＜ 0.01 ].SAPS and SANS scores of the treatment group were lower than the control group,the differences were significant [ (7.31 ± 1.51) vs ( 10.16 ± 2.10),( 12.32 ± 2.16) vs ( 15.65 ±1.48) ;t =3.62,3.12;P＜0.01].Conclusions Reward behavior therapy could help reduce some of the symptoms of chronic schizophrenia,improve self-care ability,prevent deterioration and regain mental function.

The wearable electrochemical sensors exhibit many advantages such as flexibility,miniaturization,portability,biocompatibility and low cost,and can be used for accurate,safe,real-time,and non-invasive monitoring of physiological signals in sweat,which are very helpful to monitor various physiological indicators of athletes during training,predict sports risks,prevent sports injuries and scientifically guide sports training,showing enormous potential in the field of sports monitoring. In this paper,the latest research progress of wearable electrochemical sweat sensors based on the relationship between biomarkers in sweat and exercise training was reviewed,such as the sensor substrate materials,sweat sampling strategies,sensing unit,signal transmission,and power supply systems. In addition,the current application status,the opportunities and challenges in the field of motion monitoring of wearable electrochemical sensors were discussed,and their future development was prospected.

Objective To estimate correlation between phonetically balanced maximum(PB max)and pure tone auditory threshold in auditory neuropathy(AN)patients.nethods 0ne hundred and six ANpatients were identified using multipie criteria including PB max,a metric for speech recognition,pure tone auditory threshold.acoustic emission test.distortion products otoacoustic emission(DPOAE) and auditory brainstem response(ABR).SPSS statistical software was used to estimate the Pearson's correlation between PB max and pure tone auditory threshold and to test whether pure tone auditory threshold,or auditory configuration had a significant impact on PB max.Results Even the patients had the same or similar values for pure tone auditory threshold or auditory configuration.varied values of PB max were found in two hundreds and twelve ears for 106 patients.Analysis of the data for 106 patients revealed a negative correlation(r=-0.602,P<0.01) between PB max and pure tone auditory threshold,i.e.hearing loss at a mild relates to a lower PB max.By using analysis of variance(ANOVA)method,it Was found that both pure tone auditory threshold and auditory configuration had a significant(P<0.01)impact on the patients' PB max.Conclusions This analysis implicated the promise and potential of pure tone auditory threshold and auditory configuration for predicting PB max of the AN patients,and improving the diagnosis of AN.