1.Characterization of monoclonal antibodies against human cytomegalovirus(HCMV) glycoprotein.
Eung Soo HWANG ; Ju Young SEOH ; Chung Gyu PARK ; Yoon Hoh KOOK ; Chang Yong CHA
Journal of the Korean Society for Microbiology 1992;27(6):545-553
No abstract available.
Antibodies, Monoclonal*
;
Glycoproteins*
;
Humans*
2.Immunoelectron Microscopic Analysis of Human Cytomegalovirus ( HCMV ) Antigens Reacting with Specific Monoclonal Antibodies.
Eung Soo HWANG ; Chang Yong CHA ; Hae Kyung PARK ; Ju Young SEOH
Journal of the Korean Society for Microbiology 1997;32(5):601-610
For the purpose of morphologic analysis of human cytomegalovirus (HCMV) antigens reacting with specific monoclonal antibodies, we observed HCMV particles after immunogold staining. HCMV was cultured in human fetal lung fibroblasts to be concentrated by polyethylene glycol 6,000. The HCMV stock was dropped onto Formva-coated grids and was fixed by 2% glutaraldehyde. The grids were reacted with MCMVA57, 93, 135 or with SCMVM1, 6, 14, 49 monoclonal antibodies (MoAbs) follwed by gold (10 nm)-conjugated goat anti-mouse IgG. Then the grids were stained with 2.5% uranyl acetate to be observed under Hitachi 500 or Jeol 1,200 electron microscope. When HCMV was reacted with SCMVM14 and SCMVM49 MoAbs, gold particles were adsorbed to virion envelopes, suggesting that the reactive antigens were envelope proteins. In cases of MCMVA135 and SCMVM6 MoAbs, gold particles were adsorbed to dense bodies as well as to virion envelope. These results, together with the previous results of immunologic and genetic characterization, suggested that the reactive antigens of MCMVA135 and SCMVM6 MoAbs were gB homologue and structural protein, respectively. In case of SCMVM1 MoAb, gold particles were adsorbed to capsids, envelopes, and dense bodies, suggesting that the reactive antigen was structural protein. In case of SCMVM8 MoAb, gold particles were observed between the envelopes and capsids, which space was supposed to be the tegument, suggesting that the reactive antigen was carbohydrate moiety of glycoprotein or its polymer. In cases of MCMVA57 and MCMVA93 MoAbs, gold particles were adsorbed to only dense bodies, suggesting that the reactive antigens were precursors of structural proteins.
Antibodies, Monoclonal*
;
Capsid
;
Cytomegalovirus*
;
Fibroblasts
;
Glutaral
;
Glycoproteins
;
Goats
;
Humans*
;
Immunoglobulin G
;
Lung
;
Polyethylene Glycols
;
Polymers
;
Virion
3.Investigation of antigen related to the in vitro invasiveness of salmonella typhimurium through the Madin-Darby canine kidney(MDCK) epithelial cell monolayer.
Chung Gyu PARK ; Eung Soo HWANG ; Ju Young SEOH ; Yoon Hoh KOOK ; Chang Yong CHA
Journal of the Korean Society for Microbiology 1991;26(6):553-562
No abstract available.
Epithelial Cells*
;
Salmonella typhimurium*
;
Salmonella*
4.Characterized of the gene encoding a protein recognized by human cytomegalovirus specific monoclonal antibody(MCMVA93).
Chung Gyu PARK ; Eung Soo HWANG ; Ju Young SEOH ; Seok Yong KIM ; Yoon Hoh KOOK ; Chang Yong CHA
Journal of the Korean Society for Microbiology 1993;28(6):495-504
No abstract available.
Cytomegalovirus*
;
Humans*
5.Analysis of protein antigens of varicella-zoster virus using monoclonal antibodies.
Ju Young SEOH ; Eung Soo HWANG ; Myoung Don OH ; Yoon Hoh KOOK ; Sung Bae CHOI ; Chang Yong CHA
Journal of the Korean Society for Microbiology 1993;28(2):153-163
No abstract available.
Antibodies, Monoclonal*
;
Herpesvirus 3, Human*
6.The role of CDI on the antigen recognition of human CD3+CD4-CD8 T lymphocyte clone specific to M.tyberculosis.
Myung Sik CHOI ; Ju Young SEOH ; Dong Gyun LIM ; Ik Sang KIM ; Woo Hyun CHANG ; Chang Yong CHA
Journal of the Korean Society for Microbiology 1993;28(6):505-519
No abstract available.
Clone Cells*
;
Humans*
;
Lymphocytes*
7.Megakaryocytic Differentiation of Human Cord Blood CD34+Cells During ex vivo Expansion.
So Yong KWON ; Wha Soon CHUNG ; Ju Young SEOH
Korean Journal of Hematology 2001;36(2):136-147
BACKGROUND: Thrombopoietin (TPO) has been currently used for ex vivo expansion of hematopoietic progenitor cells. Previously, we have reported that TPO induces a characteristic pattern of apoptosis during ex vivo expansion of cord blood (CB) CD34+cells. In the present study, we have investigated on the relationship between the TPO-induced apoptosis and megakaryocytic differentiation. METHODS: CD34+cells, purified from human CBs, were expanded in serum-free conditions stimulated with TPO. Multidimensional flow cytometry and TUNEL assay as well as electron microscopy were applied for analysis of apoptosis. Asociation of megakaryocytic differentiation and apoptosis was studied by FACS-sorting and immunocytochemistry. Clonogenic potential was studied by CFU-MK assay. RESULTS: The TPO-induced apoptotic cells appeared in CD61+fractions. Immunocytochemical analysis of the FACS-sorted fractions showed that the apoptosis-associated CD44low fraction expressed CD61. Clonogenic assay revealed 7.4+-0.50-fold increase of total CFU-MKs during the initial 9 days. Thereafter, the number of CFU-MKs decreased, which was parallel with the increase of apoptosis. When the MK colonies were subdivided according to size, the proportion of large colonies progressively decreased, while that of medium and small colonies increased. In particular from day 6, small colonies became predominant. CONCLUSION: These results suggested that the MK progenitors matured as they were expanded during ex vivo expansion with TPO, and then proceeded to apoptosis.
Apoptosis
;
Fetal Blood*
;
Flow Cytometry
;
Hematopoietic Stem Cells
;
Humans*
;
Immunohistochemistry
;
In Situ Nick-End Labeling
;
Megakaryocytes
;
Microscopy, Electron
;
Thrombopoietin
8.Regulatory Eosinophils in Inflammation and Metabolic Disorders.
Bo Gie YANG ; Ju Yong SEOH ; Myoung Ho JANG
Immune Network 2017;17(1):41-47
Eosinophils are potent effector cells implicated in allergic responses and helminth infections. Responding to stimuli, they release their granule-derived cytotoxic proteins and are involved in inflammatory processes. However, under homeostatic conditions, eosinophils are abundantly present in the intestine and are constantly in contact with the gut microbiota and maintain the balance of immune responses without inflammation. This situation indicates that intestinal eosinophils have an anti-inflammatory function unlike allergic eosinophils. In support of this notion, some papers have shown that eosinophils have different phenotypes depending on the site of residence and are a heterogeneous cell population. Recently, it was reported that eosinophils in the small intestine and adipose tissue, respectively, contribute to homeostasis of intestinal immune responses and metabolism. Accordingly, in this review, we summarize new functions of eosinophils demonstrated in recent studies and discuss their homeostatic functions.
Adipose Tissue
;
Eosinophils*
;
Gastrointestinal Microbiome
;
Helminths
;
Homeostasis
;
Immunoglobulin A
;
Inflammation*
;
Interleukin-4
;
Intestine, Small
;
Intestines
;
Metabolism
;
Phenotype
;
Th17 Cells
9.Focus assay for varicella-zoster virus(VZV) by immunoperoxidase staining.
Ju Young SEOH ; Bang Soon KIM ; Chung Gyu PARK ; Eung Soo HWANG ; Myoung Don OH ; Yoon Hoh KOOK ; Sung Bae CHOI ; Chang Yong CHA
Journal of the Korean Society for Microbiology 1992;27(4):365-369
No abstract available.
10.Cultural conditions for the production of high-titered varicella-zoster virus(VZV).
Ju Young SEOH ; Bang Soon KIM ; Chung Gyu PARK ; Eung Soo HWANG ; Myoung Don OH ; Yoon Hoh KOOK ; Chang Yong CHA
Journal of the Korean Society for Microbiology 1992;27(4):359-363
No abstract available.