2.Efficacy of voriconazole for treatment of invasive fungal infections in 87 cases with hematologic malignancies.
Ai-Mei FENG ; Ju-Ying WEI ; Wen-Juan YU
Chinese Journal of Hematology 2011;32(7):473-475
Adult
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Aged
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Aged, 80 and over
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Antifungal Agents
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therapeutic use
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Female
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Hematologic Neoplasms
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drug therapy
;
microbiology
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Humans
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Male
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Middle Aged
;
Mycoses
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complications
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drug therapy
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Pyrimidines
;
therapeutic use
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Treatment Outcome
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Triazoles
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therapeutic use
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Voriconazole
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Young Adult
4.Expression of mSDF-1?/GM-CSF fusion protein and its protective effect on radiotherapy-induced hematopoiesis damages
Bin XU ; Xiaoping JU ; Wei JING ; Ying CHEN
Chinese Journal of Cancer Biotherapy 1994;0(01):-
Objective: To express mSDF-1?/GM-CS fusion protein in the Pichia pastoris expression system and investigate its in vivo promoting effects on hematopoiesis function and immune function.Methods: The mSDF-1?/GM-CS fusion gene was chemically synthesized and was cloned into pichia expression vector;the vector was then transfected into Pichia pastoris expression system.Then the expressed products were detected by SDS-PAGE,Western blotting and purified by ion exchange columns.Rat irradiation model was established by 60Co ? ray and were subcutaneously injected with the fusion protein.The hematopoietic and chemotactic activities of the fusion protein were investigated in vivo.Results: The expression vector pPIC9K-SDF1-L-GM-CSF was successfully constructed,and mSDF-1?/GM-CS fusion protein was successfully expressed by Pichia pastoris strain GSl15.The molecular weight of the protein was about 32000 D,with a concentration of 78 ng/ml.The numbers of bone marrow mononuclear cells and GM-CFU were significantly increased in rats after subcutaneous injection of the fusion protein(P
5.An Preliminary Reform of the Pedagogic Modus in Teaching Pathophysiology
Qi-liang, ZHANG ; Wei, LIU ; Ju-ying, LI
Journal of Shanghai Jiaotong University(Medical Science) 2001;21(2):190-192
Objective To explore the feasibility of transform from the sardine like “cramming” to the “inductive” teaching method with provision of necessary conditions. Methods The medical students in class'97 were picked in random for the trial. New learning materials suitable for self-learning compiled by our department of pathophysiology were distributed into study to test the validity of the inductive approach. The students in this group, after self learning the meaterials, were then examined and their records were compared with those in the control group. Results 31 of 117 volunteer students in the trial participate the examination, and 54.8% of them obtained grades excellent or good (≥80 points), with a record bar surpassing the 608 students of class'96 and 560 students of class'97 (ordinary classes as control groups). Conclusion Students can master the basic knowledge of pathophysiology if proper learning materials are available, on condition they have enough time and good self-learning habits supplemented by necessary lectures and coaching.
6.High-density Expression of Recombinant Porcine Insulin Precursor by Pichia pastoris
Yu-Wei LIU ; Ming-Zhi HUANG ; Ying-Ping ZHUANG ; Ju CHU ; Si-Liang ZHANG ;
Microbiology 1992;0(01):-
The recombinaut porcine insulin precursor(PIP)produced by Pichia pastoris in shake-flask and 501.fermenter was investigated respectively.The results indicated that 60h induction time length and 2.0%~2.5% methanol addition every day was optimum in shake- flask.The process in 50L fermenter was consisted of batch,feed-batch and induction phases.The relationship between dry cell weight(y) and culture time (t) in growth phase(batch and feed-batch phase)could be described by model y=0.6525e~(0.1907t).Glycerol and ammonia were almost used for cell growth and maintain,and no by-product was observed in batch and fed-batch phase Only 80% ammonia and 70% methanol were used by cell in induction phase.By comparison the results of shake-flask and 50L fermenter,it was concluded that the limit- ing factor in the fermentation of shake-flask and 50L fermenter was dissolved oxygen(DO)and.carbon source,respectively.When scaling the result of shake-flask to 501.fermenter,the control strategy was adapted for 50L fermenter by increasing the feed rate of methanol and the maximum PIP concentration reached 1.72 g/L.
7.Identification and Screening of Broad-spectrum Antibiotics-producing Strains
Jian-Ge JU ; Hong-Wei YU ; Jun HAN ; Ying-Min JIA ;
Microbiology 1992;0(05):-
60 strains which have antimicrobial activity had been isolated from nutritious soil in China in the study.We have further selected 1 strain which product broad-spectrum antibiotics using agar well-diffusion method.The strain was identified Brevibacillus laterosporus after physiological biochemical characteristic experiments,sequencing of 16S rDNA and cluster analysis,named S62-9.
8.Assessment of gross tumor volume motion and the influence factors during respiration for lung cancer using four-dimensional computed tomography
Xiao JU ; Zongmei ZHOU ; Minghui LI ; Ke ZHANG ; Wei HAN ; Guishan FU ; Ying CAO ; Lühua WANG
Chinese Journal of Radiation Oncology 2011;20(3):198-201
Objective This study was to assess the three-dimensional gross tumor volume(GTV)motion of lung cancer caused by respiration using four-dimensional computed tomography(4DCT),and to analyze the influenee factors.Methotis Four-DCT scans of 22 lung focuses in 21 patients with lung cancer were analyzed.The gross tumor volume was contoured in all 10 respiration phases of 4DCT scans.The changes in volume of GTV,the 3D motion of the centroid,boundary of GTV and the 3D spatial motion vectors were calculated and the irdluenee factors were analyzed.Results The average change in volume of GTV was+14.3%(0.2%.42.5%)/-8.4%(0.4%-38.6%),the average movement amplitude of GTV centroid and GTV boundary were(0.18±0.12)cm,(0.20±0.16)cm,(0.53±0.59)cm and(0.42±0.23)cm,(0.41±0.22)cm,(0.57±0.70)cm in medio-lateral,vertro-dorsal,cranio-caudal(CC) direction,respectively.The CC movement was larger than other directions(Z=-2.12,P=0.034;Z:-2.10,P=0.035),and no significant difference was observed in 3D motion of GTV boundary(Z=-0.81.P=0.417;Z=-0.86,0.391).The CC motion of GTV eentroid in lower lobe was larger than that in upper lobe[(0.87±0.64)and(0.35±0.49)cm,(t=-2.12,P=0.047)],and no significant difference was found in other directions[(0.23±0.10)and(0.19±0.18)em(t=-0.49,P=0.629),(0.21±0.13)and(0.17±0.11)cm(t=0.76,P=0.460)].There was no correlation of the 3D movement and 3D spatial motion vector of GTV to the volume of GTV(r=-0.306,-0.062,-0.279,-0.300;P=0.189,0.796.0.234,0.199).Conclusions GTV motion of patients with lung cancer is individual,the CC movement is the moat obvious,using 4DCT to assess is comparatively accurate.The motion amplitude of lower lobe focuses is larger.No significant correlation of the GTV motion to the volume was observed.Larger sample study is needed to analyze the influence of adjacency to the GTV motion.
9.A child with gastric stromal sarcoma.
Shao-ming ZHOU ; Lai-bao SUN ; Hong-ying LUO ; Ju-rong WEI ; Da-ming BAI
Chinese Journal of Pediatrics 2004;42(1):73-73
Child, Preschool
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Female
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Humans
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Sarcoma
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diagnosis
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surgery
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Stomach Neoplasms
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diagnosis
;
surgery
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Stromal Cells
;
pathology
10.An investigation of dental fluorosis of children aged 8 - 12 in Wushan and Fengjie counties of Chongqing
Ju, YAN ; Zhao-hui, ZHONG ; Ying-xiong, WANG ; Xing-jian, LUO ; Wei, YAN ; Li-hong, MU
Chinese Journal of Endemiology 2012;31(4):423-425
ObjectiveTo investigate the prevalence and distribution characteristics of dental fluorosis of children aged 8 - 12 in Wushan and Fengjie counties of Chongqing and to provide a scientific basis for prevention and control of the disease.MethodsTwenty townships(towns) in Fengjie county and 18 in Wushan county were selected as survey points by random cluster sampling in 2010.Dental fluorosis of all the children aged 8 - 12 was examined with Dean index.The detection rate of children's dental fluorosis,defect rate and dental fluorosis index were compared between the two counties.Results Totally 38 209 children aged 8 - 12 were investigated.The total detection rate of dental fluorosis was 43.09%( 16 466/38 209) in the two counties.The detection rates of dental fluorosis in Wushan and Fengjie were 48.98% (9397/19 186)and 37.16%(7069/19 023),respectively,and the difference was statistically significant(x2 =544.03,P < 0.01 ).Total detection rates of dental fluorosis of the five age groups(8,9,10,11,and 12-year-old age groups) were 32.52%(2157/6632),40.07%(2672/6668),43.67%(3420/7831 ),46.01% (3861/8391) and 50.14% (4356/8687),respectively,and the difference was statistically significant (x2 =510.50,P < 0.01),Dental fluorosis indexes in Wushan and Fengjie were 0.713 and 0.485,respectively.Defect rates of dental fluorosis in Wushan and Fengjie were 4.05% (777/19 186) and 1.57%(298/19 023),respectively.Conclusions The total detection rate of dental fluorosis of the two counties is still high,which gradually increases with age.Wushan is still an endemic area of dental fluorosis,and Fengjie is at the edge of the