Objective To investigated the relationship between serum matrix metalloproteinase-9(MMP-9) and carotid atherosclerosis(AS) in type 2 diabetes mellitus(T2DM).Methods A total of 93 patients with T2DM were recruited to our study.The intima-media thickness(IMT) and plaques of carotid artery were measured.These patients were divided into 3 groups according to their IMT values: diabetes mellitus(DM) group(n=32),carotid artery intima thicken group(n=31) and carotid artery intima plaque group(n=30).At the same time,30 healthy individuals were selected as control.Serum level of MMP-9 were determined and analyzed.Results The serum MMP-9 in DM group was significantly higher than that in healthy controls([550.26±269.28]μg/L vs.[359.70±215.62]μg/L,t=2.23,P＜0.05).The serum MMP-9 level of intima thicken group(712.15±340.47)μg/L was significantly higher than that in healthy controls(t=4.53,P＜0.01) and DM group(t=2.40,P＜0.05).The serum MMP-9 level of plaque group([889.08±247.80]μg/L) was even more significantly higher than DM group(t=4.89,P＜0.01),IMT group(t=2.53,P＜0.05) and healthy controls(t=7.01,P＜0.01).Conclusion The severity of carotid atherosclerosis in T2DM is closely associated with the serum MMP-9 level.

Objective To research the forming technology of Chuansu Jiuxin dropping pills.Methods The pills were prepared by dropping method.The forming technology of the pills was optimized by single factor test and orthogonal experiment by using the amount of PEG4000 to PEG6000, ratio of drug-matrix ,dropping temperature and speed as factors and with pill weight RSD,the time limit of collapse and appearance quality of the pills as evaluation indexes.Results Using PEG 4000 and PEG 6000 as matrix with the ratio of 4:1 and dimethyl silicone oil(100 mPa· s)as refrigerant at 5~15℃,the optimized parameters for the forming technology were as follows:the ratio of herbal extract to matrix was 1:3,the dropping temperature was at 90 ℃ and the dropping speed was 35 drops per minute,dropping from 4 cm.Conclusion The optimized technology is sample and feasible,and can provide a reference for industrial production of Chuansu Jiuxin dropping pills.

Objective: To investigate the effects of chemotherapeutic drugs on CD44+CD24-/low cell subpopulation of triple-negative breast cancer. Methods: MDA-MB-231 cells were treated with paclitaxel, docetaxel, 5-fluorouracil, epirubicin, vinorelbine or gemcitabine at 0.2-, 1- and 5-fold of the drug peak plasma concentration (PPC) for 24, 48 and 72 h, respectively. The growth of MDA-MB-231 cells was detected by MTT assay. The percentage of CD44+CD24-/low cells was determined by flow cytometry (FCM) after treatment with different chemotherapeutic drugs at 1-fold of PPC for 48 h and after cell recovery. The change of percentage of CD44 +CD24-/low cell subpopulation in circulating blood from 10 patients with metastatic triple-negative breast cancer before and after chemotherapy was determined by FCM. Results: The proliferation ability of MDA-MB-231 cells was inhibited by the six chemotherapeutic drugs. The percentage of CD44+CD24-/low cells was not significantly increased especially in 5-fluorouracil-treated cell group(P<0.05) after treatment with different chemotherapeutic drugs at 1-fold of PPC. The percentage of CD44 +CD24-/low cells in circulating blood from 10 patients with metastatic triple-negative breast cancer was decreased after chemotherapy with 1-fold of PPC(P<0.05). Conclusion: The chemotherapeutic drugs of paclitaxel, docetaxel, 5-fluorouracil and epirubicin can reduce the percentage of CD44+CD24-/lowcell subpopulation, which in circulating blood can be decreased after chemotherapy in patients with metastatic triple-negative breast cancer. Copyright© 2011 by TUMOR.

Objective To explore the value of procalcitonin(PCT ) as early predictor of bloodstream infections .Methods Blood culturing and PCT detection were carried out simultaneously in 530 blood specimens collected from the First Affiliated Hospital of Xi′an Jiaotong University from January to December 2014 .All strains were identified by using automatic identification microbial an‐alyzer ,and levels of PCT were analyzed by using automatic enzyme‐linked fluorescent immune system .The diagnostic efficacy of PCT was evaluated by using the receiver operating characteristic(ROC) curve .Results A total of 77 specimens were with negative results of blood culturing ,and a total of 453 specimens were with positive results of culturing .Among those specimens with positive results of blood culturing ,there was 114 strains of gram‐positive bacteria ,306 strains of gram‐negative bacteria ,and 33 strains of fungi .ROC curve analysis showed that the area under the curve of PCT test in all pathogenic bacteria ,gram‐negative bacteria ,gram‐positive bacteria and fungi were 0 .760 ,0 .778 ,0 .741 and 0 .686 ,respectively .In maximum Youden′s indexes ,the cut off values were 0 .453 0 ,0 .683 5 ,0 .457 0 and 0 .399 5 ng/mL respectively ,and 0 .453 0 ng/mL was the threshold in diagnosis of bloodstream infec‐tion .Conclusion PCT is a good indicator for early diagnosing bloodstream infection ,and has better timeliness and higher sensitivity than blood culture .Moreover ,the diagnostic efficacy of PCT for gram‐negative bacteria bloodstream infections was better than that for gram‐positive bacteria and fungi bloodstream infections .

Coronary Vessels ; Humans ; Microcirculation

Behavior Therapy ; Coronary Disease ; prevention & control ; Humans ; Smoking Cessation

Anthrax is a fulminating infectious disease .Bacillus anthracis, as the pathogen of anthrax , is a potential ma-terial for biological warfare agents and biological terrors .Immunoprophylaxis and specific theraphies play key roles in cop-ing with anthrax threats.Researches on the mechanism of B.anthracis infection, especially the process of infection , can fa-cilitate the development of novel drugs for anthrax prevention and therapy .Herein, by reviewing research progress , the in-fection process of B.anthracis is introduced and the potential mechanism of anthrax infection is described .Furthermore, the relationship between researches on anthrax infection mechanisms and the development of drugs for anthrax prevention and therapy is also discussed .

OBJECTIVE:To establish the identification method of Rhizoma Arisaematis and content determination of flavon-oids.METHODS:TLC was used to identify Rhizoma Arisaematis with schaftoside and ischaftoside as reference substances.The content of flavonoids was determined by HPLC.RESULTS:TLC of test sample and that of control substance had same color dots.The linear range of schaftoside and isoschaftoside were 7.925～126.8 ?g?mL-1(r=0.999 9) and 3.996～63.94 ?g?mL-1(r=0.999 7) respectively.Average recoveries were 99.4% for schaftoside (RSD=2.10%,n=9) and 99.52% for isoschaftoside(RSD=2.42%,n=9).CONCLUSION:The method is simple,accurate and reproducible for the quality evaluation of Rhizoma Arisaematis.

Objective To optimize an extracting technology for Tongluo granule by orthogonal design. Methods Water-extracting fraction:with the weight of water-extracting fraction and paeoniflorin content as the indexes,extracting times,water volume and extracting time were screened by L9(34) orthogonal test,Alcohol-extracting fraction:with the weight of alcohol-extracting fraction and hesperidin content as the indexes,alcohol concentration,extracting times,alcohol volume and extracting time were screened by L9(34) orthogonal test. Results The optimal extraction conditions were as follows:water-extracting fraction: extracting 3 times with 12-fold water,1.5 hours for each time;alcohol-extracting fraction:refluxing and extracting 2 times with 10-fold 60% alcohol,1.5 hours for each time. Conclusion The results can provide theoretical basis for production of Tongluo granule.

OBJECTIVE: To study the effect of bioactive glass (BG) on the dentin bond strength and the microleakage of hybrid layer. METHODS: In the study, 30 dentin planes were prepared from the third molars with no caries and equally assigned to the control group, BG group, and sodium trimetaphosphate (STMP)-polyacrylic acid (PAA)-BG group (S-P-BG group), randomly. After etched with 35% phosphoric acid, the dentin planes of BG group were pretreated with 0.5 g/L BG, and the dentin planes of S-P-BG group were pretreated with 5% STMP, 5% PAA and 0.5 g/L BG. No additional pretreatment was done to the dentin planes of control group. Then the dentin planes were bonded using 3M Single Bond 2 adhesive to 3M Z350XT composite resin, and cut into 0.9 mm×0.9 mm column samples, which were stored at 37 ℃ artificial saliva (AS). After 24 hours, 1 month, and 3 months, the microtensile bond strength test was performed. The data were analyzed using one-way ANOVA and LSD method. The morphology of the bond fracture interface was observed with scanning electron microscope. Other 27 teeth were collected and the enamel layer and roots cut off, with the pulp chamber exposed. 0.1% rhodamine B was added to the 3M Single Bond 2 adhesive, and then the adhesive was applied to complete the bonding procedures as above. The teeth were stored in 37 ℃ AS for 24 hours, 1 month, 3 months, and then 0.1% sodium fluorescein solution was placed in the chambers and stained for 1 hour. Confocal laser scanning microscopy was used to observe the interface morphology and microleakage of the hybrid layer. RESULTS: At the end of 24 hours and 1 month, there was no significant difference in the microtensile bond strength among the three groups (P>0.05). After 3 months of soaking, the S-P-BG group [(36.91±7.07) MPa] had significantly higher microtensile bond strength than the control group [(32.73±8.06) MPa] (P=0.026); For the control group and the BG group, the microtensile bond strength significantly decreased at the end of 3 months compared with 24 hours (control group: P=0.017, BG group: P=0.01); The microtensile bond strength of S-P-BG group af the end of 3 months had no significant difference in compared with 24 hours [(37.99±7.98) MPa] (P>0.05). Observation of the fracture surface at the 24 hours showed no obvious mineralization in all the three groups. After 1 and 3 months, mineral formation was observed in BG group and S-P-BG group, and no obvious collagen exposure was observed in S-P-BG group. Confocal laser scanning microscopy revealed no obvious differences in the morphology and quantity of the resin tag in the control group, BG group and S-P-BG group. At the end of 24 hours, leakage was found in all the three groups. The microleakage of the control group increased at the end of 3 months, while the microleakage of the BG and S-P-BG groups decreased. CONCLUSION BG pretreatment of dentin bonding interface can induce mineralization at the bonding interface and reduce the microleakage of the hybrid layer; pretreating the dentin bonding interface with STMP, PAA and BG may enhance the maintaining of the dentin bonding durability.
Dentin ; Dentin-Bonding Agents ; Glass ; Resin Cements ; Tensile Strength