Implant materials, for examples, plaster of Paris, Bone cement and ceramics which are used experimentally to regain shape and function, act as a scaffold for bone growth and contribute to the healing processes at bone defect developed by trauma, infection and other causes. Therefore, the effect of plastei of Paris on the bone defects of adult rabbits may be an interesting subject, particularly in the orthopedic field. The authors carried out an experiment to study the effect of plaster of Paris on the bone defect, using 30 adult rabbits weighing fmm 3000-3500 gm, which were divided into 3 main gmups. (Each group was composed of 10 adult rabbits.). In Group 1, which was the control group, made a defect at the proximal metaphysis of the tibia, 6 mm×6 mm×6 mm in size, using a drill bit and filled by hematoma spontaneously. In Group II and Group IU, a defect was also made by the same method as in Group I and a piece of Gelfoam or plaster of Paris was inserted into the bone defect respectively. Then we studied the healing processes of the bone defect by sacrifying animals of each group at 6th and 12 th week after operation. The results of this experiment were as follows. 1. New bone formarion in the bone defect was poor in group I and Gmup Il, but it was good in Group III. 2. The plaster of Paris, filling into the defect, was absorbed and removed from the site of implantation continuously and stimulated the new bone formation. 3. The Gelfoam in Group II was more rapidly absorbed than plaster of Paris and didn't stimulate the new bone formation.
Adult ; Animals ; Bone Development ; Calcium Sulfate ; Ceramics ; Gelatin Sponge, Absorbable ; Hematoma ; Humans ; Methods ; Orthopedics ; Osteogenesis ; Rabbits ; Tibia

PURPOSES: This study examined uncertainty and nursing need according to illness phases(phase I, II, III) in cancer patients, and investigated relationships between uncertainty and nursing need. METHODS: A cross-sectional descriptive study was conducted with a sample of 121 adult cancer patients treated in two hospitals. RESULTS: As for the total score, the uncertainty was not significantly different across the subgroups by illness phase. As for the subscale score, however, two sub-scales of the uncertainty were different across the subgroups. 'The unpredictability' was highest in illness phase I and II groups, while 'the lack of information' was highest in the illness phase III group. Nursing need as the total score was not significantly different across the subgroups by illness phase. However, all subscale scores of the nursing need were significantly different across the subgroups. Educational need was highest in the illness phase I group; physical and emotional needs were highest in the illness phase III group. Uncertainty and nursing need were not related to each other at any illness phase. CONCLUSION: The results suggest that nursing need and uncertainty may change across illness phases. Clinicians need to consider this pattern in caring for cancer patients.
Adult ; Humans ; Uncertainty

To address the limitations of animal testing, scientific research is increasingly focused on developing alternative testing methods. These alternative tests utilize cells or tissues derived from animals or humans for in vitro testing, as well as artificial tissues and organoids. In western countries, animal testing for cosmetics has been banned, leading to the adoption of artificial skin for toxicity evaluation, such as skin corrosion and irritation assessments. Standard guidelines for skin organoid technology becomes necessary to ensure consistent data and evaluation in replacing animal testing with in vitro methods. These guidelines encompass aspects such as cell sourcing, culture techniques, quality requirements and assessment, storage and preservation, and organoid-based assays.

Since the concept of osseointegration has been introduced, a lot of researches were performed to achieve stronger fixation of the implant. In this study, we tried to find the different pattern of bone ingrowth after implanting pure titanium implant and Hydroxyapatite coated titanium implant in dogs. Four typed of cylindrical titanium implants (diameter 6 mm, length 1 mm) were inserted in the distal femur of adult dogs. The histological examination was done at postoperative 2, 4, 8, and 12 weeks. There was no foreign body reaction at contact site and all implants kept direct contact with bone tissue. In the light microscopic finding, after 2 weeks, the cases with Hydroxyapatite-coated Implant (HAP) showed greater formation of immature woven bone in comparison to Titanium implant. After 4 weeks, Hydroxyapatite-coated Implant (HAP) showed more mature lamellar bone. After 8 and 12 weeks, most of the woven bone was transformed into mature lamellar bone. The fluorescence microscopic finding, after 2 weeks, revealed calcein green fluorescence around implants in all cases and it was more prominent in the cases with Hydroxyapatite-coated Implant (HAP). After 4 weeks, the center of trabecula gave out green fluorescent light, the corner of them showed red fluorescent light, and they were more prominent in the cases with Hydroxyapatite-coated Implant (HAP). After 8 and 12 weeks, green fluorescence disappeared gradually, red fluorescence appeared for the most part, and there was no difference in each cases, especially in 12 weeks. As a result, we support the concept that Hydroxyapatite-coated Implant (HAP) induces faster bone ingrowth in 4 weeks than Titanium implant, and that it may be more profitable for early bone ingrowth and implant stability.
Adult ; Animals ; Bone and Bones ; Dogs* ; Durapatite ; Femur ; Fluorescence ; Foreign-Body Reaction ; Humans ; Osseointegration ; Titanium*

PURPOSE: The role of ghrelin, which promotes the secretion of growth hormone, was not well known until now. Recently it was found that the mutation of ghrelin gene is related to obesity and diabetes. This study is to find the screening method that can easily and effectively detect the polymorphism of Leu72Met in ghrelin gene of obesity patients and apply it to clinical usage. METHODS: We compared PCR-RFLP, PCR-SSCP and ARMS methodologies for analyzing of the polymorphism of Leu72Met in ghrelin gene of obesity children, and also studied the merits and demerits of these methodologies. RESULTS: In this study, we were able to find out the band of peculiar allele of Leu72Met in ghrelin gene using PCR-RFLP, PCR-SSCP and ARMS analyses. The polymorphism of Leu72Met in ghrelin gene determined by all above methodologies was in complete agreement. Compared to the PCR- RFLP and PCR-SSCP, ARMS analysis is simple, inexpensive and also consume less time. It is very sensitive to analyze the polymorphism and easy to understand the results of test. CONCLUSION: Though PCR-RFLP, PCR-SSCP and ARMS analyses were sensitive to analyze the polymorphism of Leu72Met in ghrelin gene, ARMS analysis appears to be more efficient than PCR-RFLP and PCR-SSCP. Therefore, we conclude that ARMS analysis is suitable to analyze the polymorphism of Leu72Met in ghrelin gene for large quantity of specimens.
Alleles ; Arm ; Child ; Ghrelin* ; Growth Hormone ; Humans ; Mass Screening ; Obesity ; Polymorphism, Restriction Fragment Length

BACKGROUND: Basal cell carcinoma (BCC) is the most prevalent cancer in the western world, showing a rapid increase in incidence. Activation of the Sonic hedgehog/Patched (PTCH) and is sisnalling pathway, due to PTCH inactivation, is a key event in sporadic and familial BCC development in humans and is associated with transcriptional activation of specific target genes, including GLI-1. Recently, PTCH inactivation by UV-specific mutation has been reported to develop BCC. OBJECTIVE: To evaluate the expression pattern of GLI-1 oncogene in BCC of sun-exposed and non-exposed areas of skin. METHOD: We examined 20 cases of BCC, and 4 cases of BCC occurring in sun-exposed and non-exposed areas, using an immunohistochemical method with paraffin-embedded sections. RESULTS: The results were as follows. 1. All cases of BCC showed a positive staining for GLI-1 protein. 2. In the staining pattern of GLI-1 protein, BCC in both sun-exposed and non-exposed areas showed diffuse staining throughout the tumor lobules. Moreover, the degree of staining was not found to be different between the BCC of sun-exposed and non-exposed areas. CONCLUSION: These results suggest that increased GLI-1 expression is important for BCC development. And multiple factors, including PTCH mutation due to UV irradiation, may affect GLI-1 expression.
Carcinoma, Basal Cell* ; Humans ; Incidence ; Oncogenes ; Skin ; Transcriptional Activation ; Western World

Pernicious anemia is caused by the absence of intrinsic factor from autoimmune destruction of parietal cells. Patients with pernicious anemia are predisposed to the development of gastric cancer. We report here a case of pernicious anemia with early gastric cancer. A 65 year old woman was presented with severe anemia and glossitis. Her serum cobalamin level was low, and serologic test to anti-intrinsic factor antibody and anti-parietal cell antibody were positive. Gastroscopic examination revealed an early gastric cancer and type A atrophic gastritis. This patient underwent radical subtotal gastrectomy and received daily oral vitamin B12 replacement.
Aged ; Anemia ; Anemia, Pernicious* ; Female ; Gastrectomy ; Gastritis, Atrophic ; Glossitis ; Humans ; Intrinsic Factor ; Rabeprazole ; Serologic Tests ; Stomach Neoplasms* ; Vitamin B 12

INTRODUCTION: The objective of this research was to determine the incidence of oral cancer in Korea. MATERIALS AND METHODS: The classifications of oral and maxillofacial cancer (OMFC) that we used are based on possible locations of OMFC: lip, tongue, mouth, salivary glands, tonsil, oropharynx, nasopharynx, hypopharynx, pharynx unspecified, and nose, sinuses. RESULTS: 1) There were 2,848 OMFC cases, accounting for 1.6% of all cancers. The male to female ratio was 2.72:1. 2) The estimated crude rates (CRs) were 5.7 overall, 8.4 for males, and 3.1 for females. The age-standardized incidence rates (ASRs) were 4.6 overall, 7.3 for males and 2.3 for females. 3) The incidence of mouth cancer was highest. The mouth and salivary glands were the most frequent sites for cancer among males and females, respectively. 4) Patients who were 40 years or older accounted for 91% of OMFC cases, with the highest proportion of cases in the 60-69 year-old age group for both sexes. 5) Tongue cancer was the most prevalent OMFC overall. Nasopharyngeal cancer was highest among males, and salivary gland cancer was highest among females. 6) From 2004 to 2008, the relative 5-year survival rate of OMFC patients was 57.5%. There was a trend of increasing survival among OMFC patients during the study period. The survival rate for females (69.3%) was much higher than that for males (53.1%). CONCLUSION: Social and personal efforts should be required to increase the survival rates of OMFC patients and Korean national cancer management policy should establish new measures for economic and social management and support.
Accounting ; Female ; Humans ; Hypopharynx ; Incidence ; Korea ; Lip ; Male ; Mouth ; Mouth Neoplasms ; Nasopharyngeal Neoplasms ; Nasopharynx ; Nose ; Oropharynx ; Palatine Tonsil ; Pharynx ; Salivary Gland Neoplasms ; Salivary Glands ; Survival Rate ; Tongue ; Tongue Neoplasms

PURPOSE: The purpose of this study was evaluation of the current status of medical students' documentation of patient medical records METHODS: We checked the completeness, appropriateness, and accuracy of 95 Subjective-Objective-Assessment-Plan (SOAP) notes documented by third-year medical students who participated in clinical skill tests on December 1, 2014. Students were required to complete the SOAP note within 15 minutes of an standard patient (SP)-encounter with a SP complaining rhinorrhea and warring about meningitis. RESULTS: Of the 95 SOAP notes reviewed, 36.8% were not signed. Only 27.4% documented the patient's symptoms under the Objective component, although all students completed the Subjective notes appropriately. A possible diagnosis was assessed by 94.7% students. Plans were described in 94.7% of the SOAP notes. Over half the students planned workups (56.7%) for diagnosis and treatment (52.6%). Accurate documentation of the symptoms, physical findings, diagnoses, and plans were provided in 78.9%, 9.5%, 62.1%, and 38.0% notes, respectively. CONCLUSION: Our results showed that third-year medical students' SOAP notes were not complete, appropriate, or accurate. The most significant problems with completeness were the omission of students' signatures, and inappropriate documentation of the physical examinations conducted. An education and assessment program for complete and accurate medical recording has to be developed.
Clinical Competence ; Diagnosis ; Education ; Humans ; Medical Records ; Meningitis ; Physical Examination ; Pilot Projects* ; Soaps* ; Students, Medical*

Cerebral mycotic aneurysm is an uncommon complication of infective endocarditis. In spite of low incidence, mycotic aneurysm is a serious complication because of the high risk of its rupture. Therefore, early diagnosis and management of cerebral mycotic aneurysm are imperative before the rupture. We experienced a case of asymptomatic cerebral mycotic aneurysm in a 30-year old man with infective endocarditis. He was admitted for splenic and renal infarction due to systemic embolization. The echocardiography showed severe mitral regurgitation, mitral valve prolapse and vegetation on the mitral valve. Streptococcus mitis was identified from blood cultures. In the course of antibiotics therapy, brain magnetic resonance image and 4-vessel cerebral angiography were performed, which revealed multiple unruptured cerebral mycotic aneurysms. Considering the high risk of rupture we performed mitral valve replacement with bioprosthesis. On follow-up cerebral angiography after the valve replacement surgery, one of aneurysms was enlarged, and successfully treated by surgical intervention without complication. We report one case of mycotic aneurysm without neurologic symptom in an infective endocarditis patient, who was treated without serious sequelae of the aneurysm.
Adult ; Aneurysm ; Aneurysm, Infected* ; Anti-Bacterial Agents ; Bioprosthesis ; Brain ; Cerebral Angiography ; Early Diagnosis ; Echocardiography ; Endocarditis* ; Follow-Up Studies ; Humans ; Incidence ; Infarction ; Mitral Valve ; Mitral Valve Insufficiency ; Mitral Valve Prolapse ; Neurologic Manifestations ; Rupture ; Streptococcus mitis