1.Histomorphometric evaluation of the bone surrounding orthodontic miniscrews according to their adjacent root proximity.
Hyun Ju OH ; Jung Yul CHA ; Hyung Seog YU ; Chung Ju HWANG
The Korean Journal of Orthodontics 2018;48(5):283-291
OBJECTIVE: This study was conducted to perform histomorphometric evaluations of the bone surrounding orthodontic miniscrews according to their proximity to the adjacent tooth roots in the posterior mandible of beagle dogs. METHODS: Four male beagle dogs were used for this study. Six orthodontic miniscrews were placed in the interradicular spaces in the posterior mandible of each dog (n = 24). The implanted miniscrews were classified into no loading, immediate loading, and delayed loading groups according to the loading time. At 6 weeks after screw placement, the animals were sacrificed, and tissue blocks including the miniscrews were harvested for histological examinations. After analysis of the histological sections, the miniscrews were categorized into three additional groups according to the root proximity: high root proximity, low root proximity, and safe distance groups. Differences in the bone–implant contact (BIC, %) among the root proximity groups and loading time groups were determined using statistical analyses. RESULTS: No BIC was observed within the bundle bone invaded by the miniscrew threads. Narrowing of the periodontal ligament space was observed in cases where the miniscrew threads touched the bundle bone. BIC (%) was significantly lower in the high root proximity group than in the low root proximity and safe distance groups. However, BIC (%) showed no significant differences among the loading time groups. CONCLUSIONS: Regardless of the loading time, the stability of an orthodontic miniscrew is decreased if it is in contact with the bundle bone as well as the adjacent tooth root.
Animals
;
Dogs
;
Humans
;
Male
;
Mandible
;
Periodontal Ligament
;
Tooth Root
2.Minimally Invasive Lumbar Microdiscectomy using Tubular Retractor: A Preliminary Report.
Yung PARK ; Joong Won HA ; Hyun Cheol OH ; Ju Hyung YOO ; Yun Tae LEE ; Doo Hyung LEE ; Chul Jun CHOI
The Journal of the Korean Orthopaedic Association 2005;40(6):679-685
PURPOSE: To evaluate the early clinical results of lumbar microdiscectomy using minimally invasive tubular retractor (METRx-MD system, Medtronic Sofamor Danek, Memphis, TN), and to validate the merits of minimally invasive spinal surgery. MATERIALS AND METHODS: From April, 2003 to April 2004 we retrospectively studied a consecutive series of 45 patients who underwent lumbar microdiscectomy using minimally invasive tubular retractor. In all cases, minimally invasive approach using the tubular retractor were performed with a 2 cm sized paramedian incision. The following data were collected: clinical outcomes, operative time, intraoperative blood loss, need for blood replacement, time needed before ambulation, length of hospital stay, and complications. The clinical outcomes were assessed by the modified MacNab criteria. RESULTS: Minimally invasive tubular microdiscectomy was performed in 45 patients over a 12-month period with an average follow-up of approximately 8 months. The clinical outcomes assessed by MacNab criteria were excellent in 33 patients (73%), good in 10 patients (22%). The average operative time was 63 minutes (range, 35 to 95 minutes). The average blood loss was 62 mL (range, 50 to 110 mL). None of the patients needed blood replacement. With the exception of 2 patients, all patients could walk at the day of surgery. The average hospital stay was 2.3 days. None of the patients had dural tear, wound problem, or other complications. CONCLUSION: Lumbar microdiscectomy using tubular retractor can offer a useful modality for the treatment of lumbar herniated disc with the merits of minimally invasive spinal surgery. Further long-term, randomized, prospective investigations are needed to fully evaluate the impact of this technique.
Follow-Up Studies
;
Humans
;
Intervertebral Disc Displacement
;
Length of Stay
;
Operative Time
;
Wounds and Injuries
3.Is a Suction Drain Necessary in Arthroscopic Rotator Cuff Repair?.
Jin Young PARK ; Ju Hyun SIM ; Jae Hyung LEE ; Kyung Soo OH ; Seok Won CHUNG
Clinics in Shoulder and Elbow 2016;19(3):137-142
BACKGROUND: The purpose of this study was to evaluate the efficacy of suction drain use following arthroscopic rotator cuff repair by comparing early pain score and range of motion (ROM) between groups with and without suction drains. METHODS: The study included 153 patients with rotator cuff tears who underwent arthroscopic repairs at our clinic from April 2014 to March 2015. Following surgery, a suction drain was used in 85 patients (group D) and not used in 68 patients (group ND). There was no statistical difference between the groups in terms of age, gender, or total operation time. The clinical outcome with regard to pain (assessed by pain scores and analgesic requests) and passive ROM was assessed preoperatively and postoperatively. RESULTS: Immediate postoperative analgesic requirement was significantly higher in group D (p=0.001), although there was no difference in pain outcomes between the groups during the 3-month follow-up period. A statistically significant difference in passive ROM was observed at the postoperative 2- and 6-week follow-ups (p=0.036, 0.035, and 0.034 in forward elevation (FE), external rotation at the side (ER) and 90 ER at weeks 2, respectively; 0.045 and 0.009 in FE and ER at weeks 6, respectively); however no significant difference was observed at the end of 3 months. During the study period, no complication was reported in either group. CONCLUSIONS: Use of suction drains after arthroscopic rotator cuff repair provided little benefit in terms of ROM or pain in the early postoperative period (up to 3 months).
Arthroscopy
;
Follow-Up Studies
;
Humans
;
Postoperative Period
;
Range of Motion, Articular
;
Rotator Cuff*
;
Shoulder
;
Suction*
;
Tears
4.A Clinical Analysis of Suparacondylar Fracture of the Femur in Adult
Chang Ju LEE ; Seung Rim PARK ; Jung Soo PARK ; Won Chang PARK ; Dong Sung OH ; Hyung Kyun OH
The Journal of the Korean Orthopaedic Association 1984;19(5):864-872
The treatment of supracondylar fracture of the femur remains a difficult problem because of the wide range of portential complications. In the past, authors thought conservative treatment was superior to operative treatment of supracondylar fracture of the femur. In addition, the advent of the Cast-Brace introduced the change in concept of the conservative treatment. Recently, however, the advances in the techniques and in the devices used in intemal fixation have encouraged the operative treatment. Tibiofemoral angle, gap or stepping of the articular surface of the distal femur, range of motion of the knee, and pains in the knee were used to evaluate the following results investigated by statistical study(T-test). These clinical results were shown by analyzing 49 cases of supracondylar fracture of the femur trea ted in the Department of Orthopedic Surgery, Hallym College of Medicine, from Jan. 1980 to Dec. 1983 1. We end result of each patient was evaluated by scaled score value calculated from above mentioned criteria. 2. The mean value of the operative treatment(35 cases) was 31.03±5.52, while that of the conservative treatment(14 cases) was 27.71±4.56. 3. The mean value of the group which began the exercise of the knee joint within 2 weeks (33 cases) was 32.24±4.43. The mean value of the other group which began the exercise of the knee joint after 2 weeks(16 cases) was 25.78±4.56. This difference of means between two groups was statistically significant. Beginning the knee joint exercise within 2 weeks has large effect on the result of the treatment. 4.The results of treatment in patient over age of 50 were graded as fair irrespective of methods of treatment. 5. Various devices were used in intemal fixation but there was no difference in the results. 6. We mean value of the Tibiofemoral angle(difference from normal angle) in the operative treatment was 2.37±6.25 and that of the conservative treatment was 8.04±4.62. Both of the above method of treatment showed a varus trend, especially in the conservative treatment. In statistical study, through, there was no significance in two methods.
Adult
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Femur
;
Humans
;
Knee
;
Knee Joint
;
Methods
;
Orthopedics
;
Range of Motion, Articular
;
Statistics as Topic
5.Efficacy and safety of hepatic arterial infusion chemotherapy for advanced hepatocellular carcinoma as first-line therapy.
Myung Jin OH ; Heon Ju LEE ; Si Hyung LEE
Clinical and Molecular Hepatology 2013;19(3):288-299
BACKGROUND/AIMS: Hepatic arterial infusion chemotherapy (HAIC) with 5-fluorouracil and cisplatin for intractable advanced hepatocellular carcinoma (HCC) may have survival benefits. We aimed to determine the efficacy and safety of HAIC for advanced HCC as first-line therapy. METHODS: A total of 54 patients who received only HAIC with 5-fluorouracil (750 mg/m2 on days 1-4) and cisplatin (25 mg/m2 on days 1-4) for advanced HCC from Jan. 2009 to Dec. 2011 were selected. According to Child-Pugh class, the overall survival (OS), progression-free survival (PFS), and adverse events after HAIC were investigated retrospectively. RESULTS: Median OS and PFS between the Child-Pugh A group (n=24) and the Child-Pugh B/C group (n=30) were 8.7 (95% confidence interval [CI]: 4.7-12.7) vs. 3.7 months (95% CI: 2.0-5.3), and 7.1 (95% CI: 3.8-10.4) vs. 3.6 months (95% CI: 2.0-5.2), respectively. Although median OS and PFS were not statistically significant between the two groups (P=0.079, P=0.196), the Child-Pugh class B/C tended to influence poor OS. Serious adverse events > or = grade 3 occurred frequently in both groups (83.3 vs. 96.7%, P=0.159). Responders (22.2%, complete or partial response) significantly differed in median OS, compared to non-responders (13.1 vs. 4.4 months, P=0.019). Achievement of complete or partial response was an independent prognostic factor of OS (hazard ratio: 0.4, 95% CI: 0.2-0.8, P=0.011). CONCLUSIONS: Achievement of response after HAIC provide a survival benefit in patients with advanced HCC, but HAIC should be administered cautiously in patients with Child-Pugh class B/C, because of a relatively low survival and high incidence of serious adverse events.
Adult
;
Aged
;
Anemia/etiology
;
Antineoplastic Agents/adverse effects/*therapeutic use
;
Carcinoma, Hepatocellular/*drug therapy
;
Cisplatin/adverse effects/*therapeutic use
;
Diarrhea/etiology
;
Disease-Free Survival
;
Drug Therapy, Combination
;
Female
;
Fluorouracil/adverse effects/*therapeutic use
;
Humans
;
Infusions, Intra-Arterial
;
Kaplan-Meier Estimate
;
Liver Neoplasms/*drug therapy
;
Male
;
Middle Aged
;
Neutropenia/etiology
;
Retrospective Studies
;
Severity of Illness Index
;
Thrombocytopenia/etiology
;
Treatment Outcome
6.Hydroxyl Radical Production after Intrastriatal Injection of Dopamine and the Effect of Growth Hormone on the Apoptosis of Striatal Neurons Injured by Hypoxia-ischemia in Newborn Rat Brain.
Jae Ju CHO ; Jeesuk YU ; Youn Hee JEE ; Soon Bum LEE ; Soo Yeun OH ; Hyung Gun KIM ; Young Pyo CHANG
Journal of Korean Society of Pediatric Endocrinology 2004;9(2):145-151
PURPOSE:We investigated the production of oxygen hydroxyl radicals in the striatum of neonatal rat brain after intrastriatal injection of dopamine (DA) and the effect of growth hormone (GH) on the apoptosis of striatal neurons injured by hypoxia-ischemia. METHODS:The extracellular striatal levels of 2,3-dihydroxybenzoic acid (DHBA) and 2,5-DHBA as indicators of hydroxyl radical(OH-) production were measured by in vivo microdialysis in the striatums of 7 day-old newborn rats (n=10) after direct intrastriatal infusion of dopamine hydrochloride (1.0 micromol/microL). The samples of perfused artificial cerebrospinal fluid (CSF) were collected every 10 minutes interval. The levels of DA, 2,3-DHBA and 2,5-DHBA of CSF were analysed by HPLC (high performance liquid chromatography). Also, the brains were removed at 24 hour after hypoxic-ischemic injury by Rice-Vannucci method. The coronal sections (12 micrometer) of paraffin-fixed brains were stained by TUNEL (terminal transferase-mediated dUTP nick-end-labelling) technique, and the neuronal cells undergoing apoptosis in the striatum were observed by fluorescent microscopy and compared between GH-treated (50 mg/kg, Dong-Ah Pharmacy Co.) and saline-treated rats. RESULTS:The extracellualr striatal levels of 2,3-DHBA and 2,5-DHBA increased abruptly in the first 10 minutes samples after intrastriatal injection of DA. After then, the levels declined slowely. The levels of striatal extracelluar 2.3-DHBA increased up to 621.8+/-508.7% of basal levels (P<0.05), and the levels of 2.5-DHBA increased up to 262.8+/-198.1% of basal levels (P<0.05). GH reduced markedly the number of apoptotic neuronal cells in the striatum after hypoxic-ischemic brain injury. CONCLUSION: The level of hydroxyl radicals increased abruptly after intrastriatal injection of DA and GH reduced markedly the number of apoptotic neuronal cells in the striatum after hypoxic-ischemic brain injury.
Animals
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Apoptosis*
;
Brain Injuries
;
Brain*
;
Cerebrospinal Fluid
;
Chromatography, High Pressure Liquid
;
Dopamine*
;
Growth Hormone*
;
Humans
;
Hydroxyl Radical*
;
In Situ Nick-End Labeling
;
Infant, Newborn*
;
Microdialysis
;
Microscopy
;
Neurons*
;
Oxygen
;
Pharmacy
;
Rats*
7.Gene Expression Profile in Microglia following Ischemia-Reperfusion Injury.
Ju Hyeon OH ; Hyung Soo HAN ; Jae Sik PARK
The Korean Journal of Physiology and Pharmacology 2006;10(4):173-180
Microglial activation is thought to play a role in the pathogenesis of many brain disorders. Therefore, understanding the response of microglia to noxious stimuli may provide insights into their role in disorders such as stroke and neurodegeneration. Many genes involved in this response have been identified individually, but not systematically. In this regards, the microarray system permitted to screen a large number of genes in biological or pathological processes. Therefore, we used microarray technology to evaluate the effect of oxygen glucose deprivation (OGD) and reperfusion on gene expression in microglia under ischemia-like and activating conditions. Primary microglial cultures were prepared from postnatal mice brain. The cells were exposed to 4 hrs of OGD and 1 h of reperfusion at 37 degrees C. Isolated mRNA were run on GeneChips. After OGD and reperfusion, > 2-fold increases of 90 genes and > 2-fold decrease of 41 genes were found. Among the genes differentially increased by OGD and reperfusion in microglia were inflammatory and immune related genes such as prostaglandin E synthase, IL-1beta, and TNF-alpha. Microarray analysis of gene expression may be useful for elucidating novel molecular mediators of microglial reaction to reperfusion injury and provide insights into the molecular basis of brain disorders.
Animals
;
Brain
;
Brain Diseases
;
Gene Expression*
;
Glucose
;
Mice
;
Microarray Analysis
;
Microglia*
;
Oxygen
;
Pathologic Processes
;
Reperfusion
;
Reperfusion Injury*
;
RNA, Messenger
;
Stroke
;
Transcriptome*
;
Tumor Necrosis Factor-alpha
8.Genotype of Varicella Zoster Virus Isolated from Korean Elderly Patients with Herpes Zoster.
Young Ju CHOI ; Kye Hyung KIM ; Myoung Don OH
Infection and Chemotherapy 2010;42(3):162-170
BACKGROUND: Herpes zoster develops via reactivation of the latent varicella zoster virus (VZV) in neuronal ganglia as host immunity declines. In Korea, seroprevalence of VZV is very high and population at risk for herpes zoster is increasing. The goal of this study is to evaluate the infection rate of the VZV and the lifetime prevalence of herpes zoster, and to determine the genotype of VZV. MATERIALS AND METHODS: Serum IgG antibody titer was measured in 399 patients. Lifetime prevalence of herpes zoster was evaluated through a survey of 2,054 participants. VZV was isolated by cell culture technique using MRC-5 cells. To determine the genotype of VZV, ORF 22, 38, 54, 62 were amplified by PCR, and after digestion of the PCR products with enzymes pstI, bglI, and smaI, restriction-fragment-length-polymorphism (RFLP) was analysed. The amplified ORF 22 PCR product was sequenced and checked for single nucleotide polymorphisms. RESULTS: The overall seroprevalence of VZV IgG in adults was 93.9% (375/399). The overall lifetime prevalence of herpes zoster was 13.7% (282/2,054). Of the patients with herpes zoster, 17.7% (50/282) of patients experienced postherpetic neuralgia for more than one month. All 22 VZV isolates were of J genotype; 21 (95.4%) isolates were all pstI+ bglI+ smaI-, and 1 (4.5%) isolate was pstI- bglI+ smaI- (pOka) genotype. CONCLUSIONS: The seroprevalece of VZV IgG antibody was 93.9% in adults. The lifetime prevalence of herpes zoster was 13.7%. The genotype of VZV isolated from adults over 60 years old were all of J genotype.
Adult
;
Aged
;
Animals
;
Cell Culture Techniques
;
Chickenpox
;
Digestion
;
Ecthyma, Contagious
;
Ganglia
;
Genotype
;
Herpes Zoster
;
Herpesvirus 3, Human
;
Humans
;
Immunoglobulin G
;
Korea
;
Neuralgia, Postherpetic
;
Neurons
;
Polymerase Chain Reaction
;
Polymorphism, Single Nucleotide
;
Population Characteristics
;
Prevalence
;
Seroepidemiologic Studies
9.Experimental Study on Transverse-Axis Neovascularization of Acellular Dermal Matrix Using Platelet-Rich Plasma
Hyung Suk YI ; Ho Young IM ; Jin Hyung PARK ; Yoon Soo KIM ; Sun-Ju OH ; Hong Il KIM
Journal of Wound Management and Research 2024;20(1):29-35
Background:
Acellular dermal matrices (ADMs) have shown promise in promoting tissue regeneration; however, their integration in challenging cases with limited vertical-axis neovascularization remains difficult. This study investigates whether clinically meaningful transverse-axis neovascularization is identified in ADM engraftment and whether this can be further accelerated by platelet-rich plasma (PRP).
Methods:
ADM and PRP-soaked ADM were implanted into New Zealand rabbits, and histological analysis was performed at different periods to compare neovascularization.
Results:
Histological analysis revealed that among 32 biopsy sites, there was transverse-axis neovascularization with an average length of 606.89 μm. When divided into two groups for assessing the impact of PRP on transverse-axis neovascularization, the extent of such neovascularization was measured as 582.99 μm in the control group and 630.79 μm in the experimental group. However, there was no statistically significant difference between the two groups (P=0.693).
Conclusion
The results of the present study indicate that achieving meaningful transverse-axis neovascularization in implanted ADMs remains a challenge, warranting further investigation into ADM structure and vertical-axis neovascularization.
10.Experimental Study on Transverse-Axis Neovascularization of Acellular Dermal Matrix Using Platelet-Rich Plasma
Hyung Suk YI ; Ho Young IM ; Jin Hyung PARK ; Yoon Soo KIM ; Sun-Ju OH ; Hong Il KIM
Journal of Wound Management and Research 2024;20(1):29-35
Background:
Acellular dermal matrices (ADMs) have shown promise in promoting tissue regeneration; however, their integration in challenging cases with limited vertical-axis neovascularization remains difficult. This study investigates whether clinically meaningful transverse-axis neovascularization is identified in ADM engraftment and whether this can be further accelerated by platelet-rich plasma (PRP).
Methods:
ADM and PRP-soaked ADM were implanted into New Zealand rabbits, and histological analysis was performed at different periods to compare neovascularization.
Results:
Histological analysis revealed that among 32 biopsy sites, there was transverse-axis neovascularization with an average length of 606.89 μm. When divided into two groups for assessing the impact of PRP on transverse-axis neovascularization, the extent of such neovascularization was measured as 582.99 μm in the control group and 630.79 μm in the experimental group. However, there was no statistically significant difference between the two groups (P=0.693).
Conclusion
The results of the present study indicate that achieving meaningful transverse-axis neovascularization in implanted ADMs remains a challenge, warranting further investigation into ADM structure and vertical-axis neovascularization.