1.Timed Analysis of Mouse 2 - Cell Embryo Development in Dulbecco's Modified Eagle Medium ( DMEM ) in The Presence of Glutamine With of Without Glucose.
Sang S CHUN ; Kee S PARK ; Taek H LEE ; Ju H KIM ; Hai B SONG
Korean Journal of Obstetrics and Gynecology 2000;43(3):395-399
OBJECTIVE: To evaluat the effects of a culture medium with glucose in the presence of glutamine on the development of mouse embryos. METHODS: Two-cell embryos recovered from ICR mice at 48 hrs after hCG injection (mated just after hCG injection) were cultured in DMEM (with 20% hFF) supplemented with or without glucose on the presence of glutamine. Embryos were cultured under three different glucose regimens: (1) 0 mM (control); (2) 0.5 mM (group I); or (3) 3.15 mM (group II), and were analyzed at 24, 48, 72 and 96 hours intervals. Chi-square test (x2-test) was used to compare values of groups. RESULTS: No differences were found in the number of embryos showing morula (control: 37.5%; group I: 51.0%; group II: 48.4%), blastocyst (control: 21.5%; group I: 33.3%; group II: 34.4%) and blastocyst and hatching or hatched blastocyst (control: 81.9%; group I: 83.3%; group II: 82.8%) between groups at 24 hrs, 48 hrs or 72 hrs respectively. However at 96 hrs, the number of hatched and attached blastocyst was significantly higher in group I (82.3%) and II (78.5%) than control (63.2%; P<0.05). CONCLUSION: The addition of glucose (0.5 mM) to the DMEM, as energy source, improved the rate of development of late stage embryos in mice.
Animals
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Blastocyst
;
Eagles*
;
Embryonic Development*
;
Embryonic Structures*
;
Female
;
Glucose*
;
Glutamine*
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Mice*
;
Mice, Inbred ICR
;
Morula
;
Pregnancy
2.Some epidemiological factors in hydatidiform mole.
Soo Mee LEE ; Eun Ju KIM ; Kyung Hee RHO ; Jee Yeon KIM ; Barbara H MARTIN
Korean Journal of Obstetrics and Gynecology 1993;36(7):1594-1601
No abstract available.
Female
;
Hydatidiform Mole*
;
Pregnancy
3.A chanting picture of eclampsia - Pusan, 1967-1989.
Eun Ju KIM ; Su Mee LEE ; Kyung Hee RHO ; Jun Hee YUN ; Barbara H MARTIN
Korean Journal of Obstetrics and Gynecology 1993;36(7):1561-1570
No abstract available.
Busan*
;
Eclampsia*
;
Female
;
Pregnancy
;
Singing*
4.Three Cases of Local Excision of Uterine Choriocarcinoma Lesion.
Nam Hee LEE ; Jin Hee SONG ; Mee Gyeong JEON ; Jeong Ju MOON ; Barbara H MARTIN ; Jeong Hae KIM
Korean Journal of Obstetrics and Gynecology 1997;40(8):1808-1813
In treating young women for gestational trophoblastic tumor ( G.T.T. ), all efforts are made to preserve fertility and hysterectomy is avoided unless absolutely essential. However, in some cases the operation may be lifesaving. During the last 20 years among the 310 cases of G.T.T. treated at Il Sin Christian Hospital, there were 3 cases in which hysterectomy was avoided by the excision of a localized uterine lesion thus preserving fertility.
Choriocarcinoma*
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Female
;
Fertility
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Humans
;
Hysterectomy
;
Pregnancy
;
Trophoblastic Neoplasms
5.Increased Neuronal and Glial Poly (ADP-Ribose) Polymerase Immunoreactivity in the Brain of Sporadic Amyotrophic Lateral Sclerosis.
Seung Hyun KIM ; Jozsef I ENGELHARDT ; Ju Han KIM ; Gu KONG ; Stanley H APPEL
Journal of the Korean Neurological Association 2002;20(6):682-693
BACKGROUND: Over activation of the DNA repairing enzyme, poly (ADP-ribose) polymerase (PARP) in response to oxidative damage of DNA appears to play a role in cellular death in neurodegenerative diseases. Previous data suggested that PARP immunoreactivity (IR) was increased in the white and gray matter in spinal cord of the sporadic amyotrophic lateral sclerosis (sALS), predominantly in cells with astroglial morphology. METHODS: In the present study, we evaluated whether the PARP expression was present widespread in various regions of brain tissue including the motor cortex, parietal cortex and cerebellum. RESULTS: By western blot, PARP-IR in motor cortex from sALS patients, compared to the same region from age-matched normal controls, was also significantly increased (p=0.006). Importantly, PARP-IR was also increased in the parietal cortex, and cerebellum of sALS patients compared to the controls, in regions that are usually clinically unaffected in ALS (p=0.043, p=0.035, respectively). In addition, increased PARP expression in ALS was more prominent compared to Alzheimer's brain. Immunohistochemistry revealed that PARP staining was more significant in the cortical neurons and in the subcortical white matter glial cells from sALS patients compared to normal controls and Alzheimer's disease. CONCLUSIONS: The data demonstrate that increase in PARP-IR is not limited only to the vulnerable motor cortex. Furthermore, PARP-IR is present in both cortical neuronal and subcortical glial cells. The data suggest that widespread cellular stress on neuronal and glial cells is present in the brain of sporadic ALS patients.
Alzheimer Disease
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Amyotrophic Lateral Sclerosis*
;
Blotting, Western
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Brain*
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Cerebellum
;
DNA
;
DNA Repair
;
Humans
;
Immunohistochemistry
;
Motor Cortex
;
Motor Neurons
;
Neurodegenerative Diseases
;
Neuroglia
;
Neurons*
;
Rabeprazole
;
Spinal Cord
6.Viriditoxin Induces G2/M Cell Cycle Arrest and Apoptosis in A549 Human Lung Cancer Cells.
Ju Hee PARK ; Tae Hwan NOH ; Haibo WANG ; Nam Deuk KIM ; Jee H JUNG
Natural Product Sciences 2015;21(4):282-288
Viriditoxin is a fungal metabolite isolated from Paecilomyces variotii, which was derived from the giant jellyfish Nemopilema nomurai. Viriditoxin was reported to inhibit polymerization of FtsZ, which is a key protein for bacterial cell division and a structural homologue of eukaryotic tubulin. Both tubulin and FtsZ contain a GTP-binding domain, have GTPase activity, assemble into protofilaments, two-dimensional sheets, and protofilament rings, and share substantial structural identities. Accordingly, we hypothesized that viriditoxin may inhibit eukaryotic cell division by inhibiting tubulin polymerization as in the case of bacterial FtsZ inhibition. Docking simulation of viriditoxin to beta-tubulin indicated that it binds to the paclitaxel-binding domain and makes hydrogen bonds with Thr276 and Gly370 in the same manner as paclitaxel. Viriditoxin suppressed growth of A549 human lung cancer cells, and inhibited cell division with G2/M cell cycle arrest, leading to apoptotic cell death.
Apoptosis*
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Cell Cycle Checkpoints*
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Cell Cycle*
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Cell Death
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Cell Division
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Eukaryotic Cells
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GTP Phosphohydrolases
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Humans*
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Hydrogen
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Lung Neoplasms*
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Lung*
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Paclitaxel
;
Paecilomyces
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Polymerization
;
Polymers
;
Tubulin
7.Anti-metastatic Effect of Natural Product-motivated Synthetic PPAR-γ Ligands
Dan-dan LI ; Ying WANG ; Zhiran JU ; Eun La KIM ; Jongki HONG ; Jee H. JUNG
Natural Product Sciences 2022;28(2):80-88
Colorectal cancer is one of the most common cancers globally, ranking second for the number of cancer-related deaths. Metastasis has been reported as the main cause of death in patients with colorectal cancer. Peroxisome proliferator-activated receptor gamma (PPAR-γ) is a transcription factor that functions as a tumor suppressor by inhibiting cellular proliferation, migration, and invasion. In our previous efforts to generate natural product-motivated PPAR-γ ligands, the compounds 1 and 2 were obtained. These compounds activated PPAR-γ and inhibited the migration and invasion of HCT116 colorectal cancer cells, and they were also found to inhibit the epithelial-to-mesenchymal transition, which is a key process in cancer metastasis. Compounds 1 and 2 upregulated expression of the epithelial marker (E-cadherin), and downregulated expression of the mesenchymal marker (N-cadherin) and transcriptional factor (Snail). Therefore, the PPAR-γ agonists 1 and 2 could serve as a valuable model for the study on anti-metastatic leads for the treatment of colorectal cancer.
8.Poly (ADP-Ribose) Polymerase immunoreactivity in Motor Neurons and Astrocytes in the Spinal Cord of Sporadic Amyotrophic Lateral Sclerosis Patients.
Seung Hyun KIM ; Jenny S HENKEL ; Ju Han KIM ; Myung Ho KIM ; Jozsef I ENGELHARDT ; Laszlo SIKLOS ; Gu KONG ; Stanley H APPEL
Journal of the Korean Neurological Association 2002;20(6):668-681
BACKGROUND: The evidence for increased oxidative stress and DNA damage in amyotrophic lateral sclerosis (ALS) prompted studies to determine if the expression of poly (ADP-ribose) polymerase (PARP) is increased in ALS. METHOD: Twenty Spinal cord specimens were obtained at the autopsy of sALS patients (n=11) and age-matched controls with non-neurological diseases (n=9). RESULTS: Using western analyses of postmortem tissue, we demonstrated that PARP-immunoreactivity (PARP-IR) was increased three-fold in spinal cord tissues of sporadic ALS (sALS) patients compared with non-neurological disease controls. Despite the increased PARP-IR, PARP mRNA expression was not increased significantly. Immunohistochemical analyses revealed PARP-IR was increased in both white and gray matter of sALS spinal cord. While PARP-IR was predominantly seen in astrocytes, large motor neurons displayed reduced staining compared with the controls. PARP-IR was increased in the pellet fraction of sALS homogenates compared with the control homogenates, representing potential PARP binding to chromatin or membranes and suggesting a possible mechanism of PARP stabilization. CONCLUSIONS: The present results demonstrate glial alterations in sALS tissue and support the role of glial alterations in sALS pathogenesis.
Amyotrophic Lateral Sclerosis*
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Astrocytes*
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Autopsy
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Chromatin
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DNA Damage
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Humans
;
Membranes
;
Motor Neurons*
;
Oxidative Stress
;
RNA, Messenger
;
Spinal Cord*
9.Sonic Hedgehog Protein Expression in Various Thyroid Tissues and Its Clinical Implication.
Kuhn Soo RYU ; Ok Jun LEE ; Wun Jae KIM ; Sung Su PARK ; Dong Ju KIM ; Jin Woo PARK ; Jae Woon CHOI ; Lee Chan JANG ; Orlo H CLARK
Korean Journal of Endocrine Surgery 2011;11(4):234-241
PURPOSE: The Hedgehog (Hh) signaling pathway is important in embryonic development including cell differentiation and proliferation. Recently, activation of this pathway has been implicated in several forms of solid cancers. We investigated sonic hedgehog (Shh) protein expression and its relation to differentiation and clinicopathologic characteristics in thyroid cancer cell lines and tissues. METHODS: FTC-236, FTC-238, and XTC-1. We made tissue microarray slides using 80 thyroid surgical specimen: 40 benign and 40 malignant lesions. Immunohistochemical staining was performed using anti-Shh antibody. mRNA expression of NIS, thyroglobulin, and CD97 were evaluated by RT-PCR. Cyclopamine was used as a Shh signal inhibitor. RESULTS: Shh expression was more prominent in TPC-1, FTC-133, and XTC-1 cell lines than the others. Cyclopamine downregulated CD97 and upregulated thyroglobulin mRNA expression, but did not induce mRNA expression of NIS. Thyroid tissues showed varied expression of Shh in both benign and malignant diseases. Shh expression was detected in 38 of 50 (76%) normal, in 18 of 25 (72%) non-neoplastic benign, in nine of 15 (60%) benign tumors, and in 31 of 40 (77%) malignant tumors. Shh over-expression was significantly less frequent in papillary thyroid carcinomas than in normal or benign thyroid tissues. In addition, Shh protein expression did not relate to clinicopathologic characteristics in papillary thyroid carcinomas. CONCLUSION: Thyroid tissues and cell lines vary in expression of Shh. Cyclopamine can induce redifferentiation in thyroid cancer cell lines. Shh protein expression, however, is unrelated to clinicopathologic characteristics in papillary thyroid carcinomas.
Cell Differentiation
;
Cell Line
;
Embryonic Development
;
Female
;
Hedgehog Proteins*
;
Hedgehogs
;
Pregnancy
;
RNA, Messenger
;
Thyroglobulin
;
Thyroid Gland*
;
Thyroid Neoplasms
10.MR Imaging Findings of Orbitofacial Infarction Secondary to Rhinoorbital Mucormycosis: A Case Report.
Jong Kwan JOO ; Jae Hee LEE ; Eun Ju JEON ; Kyong Mee KIM ; Ki Jun KIM ; Sung Yong LEE ; Kyu H CHOI
Journal of the Korean Radiological Society 2000;42(2):231-234
Rhino-orbital mucormycosis is the most frequently fatal fungal infection commonly occurring in patients with poorly controlled diabetes mellitus and and those who are immunocompromised, and requires prompt treatment. We describe a case of rhino-or-bital mucormycosis with orbital cellulitis and paranasal sinusitis, as seen on initial MR images, which on follow-up images had evol ved to orbitofacial infarction. MR imaging was useful for the demonstration of orbitofacial infarction, seen as areas of lack of enhancement and thus suggesting vascular invo l vement by mucor hyphae.
Diabetes Mellitus
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Follow-Up Studies
;
Humans
;
Hyphae
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Infarction*
;
Magnetic Resonance Imaging*
;
Mucor
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Mucormycosis*
;
Orbital Cellulitis
;
Sinusitis