Objective To investigate the characteristics of glucose and lipid metabolism in the male primary aldosteronism(PA) patients .Methods 125 male PA patients screened out by using plasma aldosterone/renin ratio(ARR) plasma aldosterone concen‐tration measurement were enrolled in the study(PA group) ,at the same time 127 male patients with essential hypertension(EH) were also recruited as EH group ,who had similar blood pressure levels and hypertension course .Clinical data and biochemical indi‐cators of each group were collected and the characteristics of glucose and lipid metabolism in the male PA patients were analyzed . Results Serum potassium level of PA group was lower than EH group(P<0 .05) .Compared with EH group ,there were significant increase of triglycerides concentration in PA group(P<0 .05) ,and significant decrease of HDL‐C concentration in PA group(P<0 .05) .Oral glucose tolerance test(OGTT) 2 h glucose level and waist in PA group were higher or larger than that in EH group (P<0 .05) .Conclusion Compared with EH patients ,glucose and lipid metabolism disorder are more severe in male PA patients , the symptoms are mainly abdominal obesity and impaired glucose tolerance .

Adult ; Female ; Humans ; Male ; Middle Aged ; Occupational Exposure ; analysis ; Pneumothorax ; complications ; etiology ; Silicon Dioxide ; analysis ; Silicosis ; complications ; etiology

OBJECTIVE: To synthesize a kind of novel cytotoxin with multi-drug-resistance reverting properties and study their cytotoxicity. METHODS: The target compounds were obtained from N-benzyl-4-piperidones and benzaldehyde derivatives on the condition of 20°C, 7-8 h, taking dry hydrogen chloride as catalyst, using aldol condensation reaction, inspected by TLC and purified through recrystallization. RESULTS: Five N-benzyl-3, 5-bis(arylmethylene)-4-piperidone derivatives were synthesized. The yield was over 49%. Their structures were characterized by 1H-NMR, ESI-MS with melting points. The data of cytotoxicity was obtained. CONCLUSION: The synthetic route is convenient and efficient; The compounds had good inhibiting activity for a few kinds of tumor cells. Copyright 2012 by the Chinese Pharmaceutical Association.

To investigate the relationship between the diameter of abdominal aortic aneurysm(AAA) and the concentration of elastin, forty five healthy male Wistar rats were selected,5 rats of them were used as normal control, the other 40 rats were randomly divided into 2 groups. A 1 cm segment of the abdominal aorta of the rat was isolated and perfused with pancreatic elastase in the experimental group, and the control group was given normal saline solution. Laparotomy was performed immediately after operation and on the 2, 7, 14 day, the aortas were measured and harvested. Histological studies were carried out to analyze the changes in elastin. The aortic diameter progressed to aneurysmal dimension in the experimental group. Histological study revealed loss of elastin in tunica media of the aorta in the experimental group. Neither macroscopic nor microscopic lesions were found in control group. It is suggested that there is a significant correlation between the aortic diameter and the decreased elastin content.

SiRNA comes from double-stranded RNA,which is processed into a small molecular fragment by Dicer.21-25nt siRNA,as the key effector molecules to the RNAi process,can inhibit gene expression with high specificity and high efficiency in mammalian cells.Currently,RNAi has been widely applied in a variety of cancer.RNAi has many active research explorations of the tumor development,metastasis and treatment in gastrointestinal cancer.

Objective To investigate the clinical value of 99mTc-MDP SPECT/CT whole body bone imaging in the diagnosis of bone metastasis of prostate cancer. Methods A total of 107 cases with prostate cancer were di-agnosed by SPECT whole body bone imaging from January 2013 to November 2016. SPECT/CT imaging of some ab-normal density collective focus found in whole body bone imaging were further carried out. Six months later ,whole body bone imaging and SPECT/CT were made again for review. In all diagnosis above ,99mTc-MDP were selected as bone imaging agent. According to the results of follow-up visit clinical and imaging diagnosis ,diagnostic perfor-mance indicators of whole body bone imaging and SPECT/CT were calculated. Results The different part of whole body bone imaging and SPECT/CT in the diagnosis of bone metastases was statistically significant(x2 = 23.000, P < 0.001). The diagnosis specificity and coincidence rate of SPECT/CT for bone metastasis were 100.0% and 98.1% respectively and they were significantly higher than those of whole body bone imaging(65.0% and 80.4%respectively). The differences were statistically significant(P < 0.05). After SPECT/CT diagnosis,a total of 193 undetermined lesions were found and 83 lesions of these were final diagnosed of bone metastases. Pelvic lesions were most in bone metastases ,accounting for 50.6%. Conclusion Whole body bone imaging can effectively determine the undetermined lesions , further SPECT/CT can significantly improve the diagnostic accuracy of prostate cancer bone metastasis.

Objective To investigate the number and activity changes of endothelial progenitor cells(EPCs) in hemodialysis patients,and explore its correlation with the risk factors of coronary heart disease.Methords Total mononuclear ceils(MNCs) were isolated from peripheral blood of patients with chronic renal failure in long-term hemodialysis and from a normal control group by Ficoll density gradient centrifugation and then were plated on humanfibronectin-coated dishes.Mter 7 days of culture,EPCs were characterized as adherent cells by double staining with FITC-UEA-I and DI-LDL,and were further identified by the expression of CD34,CD133 and KDR with flow cytometry.EPCs migration was determined with modified Boyden chamber assay.EPCs adhesive assay was performed by replanting EPCs on humanfibronectin-coated plates and then counting the adherent cells.The relationship of the EPCs' number and activity with Framingham Risk Score of ten years was also be assessed.Results Number of EPCs and the migratory & adhesive capacity were significantly lower in patients than in the control(P

Objective To construct a prokaryotic expression vector of cystatin C (Cys C), purify Cys C protein produced by the expression system, and prepare its antiserum. Methods Total RNA was isolated from HL-60 cells, and human Cys C gene was amplified with RT-PCR. The cDNA fragment was cloned into pMD18-T vector and which was confirmed by sequencing. The enzyme-digested target fragment was cloned into PET-32(a) expression vector and transfected into E.coli. BL 21(DE3), in which Cys C expression was induced. After the inclusion body protein was purified through Ni2+ affinity chromatography, processed by dialysis, identified by Western blotting, a rabbit was immunized with the fusion protein, and the antiserum was obtained. Results The result of DNA sequence analysis showed that the cloned Cys C gene sequence was completely corresponding to GenBank data. SDS-PAGE and Western blotting showed that the expressed Cys C fusion protein was about 35?103, mainly existing in the inclusion body of E.coli., that could be purified through Ni2+ affinity chromatography. The titer of the antiserum to the purified protein was 1∶8 000 by ELISA, and Western blotting confirmed that the antiserum reacted specifically to the Cys C protein. Conclusion A recombinant Cys C protein and the specific polyclonal antibody have been obtained, which provides a basis for establishment of immunoassays of human Cys C.

Objective To reassess the value of CT and clinical criteria as prognostic and severity indicators in acute pancreatitis and to investigate the correlation between them.Methods Of 65 cases of acute pancreatitis,the hospitalization days,fevering days and entire complications (including local and systemic complications)were regarded as clinical endpoints.CT criteria included Balthazar's plain CT scan score,necrosis score,CT severity index(CTSI) and London's PSI score.Clinical criteria included Ranson and APACHE Ⅱscore.Using analysis of variance,t-test and multiple linear regression analysis,the correlation between each criteria and the three clinical endpoints was examined as well as the relation between CT and clinical criteria.The power of each criteria and combination of CT and clinical criteria in predicting entire complications of AP was assessed and compared by using ROC analysis.Results The mean scores of PSI,Ranson and APACHE Ⅱamong three groups classified according to entire complications were significantly different.Except Balthazar's plain CT scan criteria,each criteria's mean scores in group with local complications was signifiantly higher than that in group without and entire complications was significantly more in sever group than that in mild group.Mean hospitalization days and fevering days were significantly longer in sever group than that in mild group with Ranson Score.PSI and Ranson score had linear correlation with fevering days,and Ranson score had linear correlation with hospitalization days.In CT criteria,a linear correlation was only found between PSI and Ranson score.ROC analysis showed the Az of Ranson score was the largest,and there was no increase in the Az when CT criteria were added to clinical criteria.Conclusion The predictive value of Ranson score and PSI are superior to that of others.Clinical criteria is superior in predicting systemic complications,whereas CT is superior in predicting local complications.There is no improvement in predicting entire complications of AP when CT criteria are added to clinical criteria.The findings of plain CT scan is found to be some laggard compared with that of clinic.CT scan and short follow-up are important in the evaluation of AP.

The somatotopical pattern of the spinal projection to the lateral reticular nucleus (LRN) was examined in 16 rabbits. The anterograde transport of HRP method were used.1. Cervical, thoracic and lumbar segments all gave rise to small numbers of fibers projecting to bilateral lateral reticular subtrigeminal subnucleus (Lrs).2. Cervical, thoracic and lumbar projections to the LRN were bilateral but the cervical ascending fibers terminated predominately on the ipsilateral side. The lumbar ascending fibers projected chiefly to the contralateral side. The thoracic cord gave fibers to bilateral LRN. No significant difference could be seen between the two sides. There were certain overlapping among the distribution areas of the terminal branches from different parts of the cord.3. The spinal projections of the rabbit were predominately terminated in the caudal half of the LRN. It showed a somatotopical pattern. The cervical cord projected to the lateral 3/5 of the lateral reticular magnocellular subnucleus (Lrm) and its neighbouring part of the lateral reticular parvocellular subnucleus (Lrp). The thoracic cord projected to the medial 3/5 of the Lrm and its neighbouring part of Lrp. The lumbar cord gave afferents to the Lrp and its neighbouring part of Lrm.