BACKGROUND:Coculture of bone marrow mesenchymal stem cel s and human umbilical vein endothelial cel s can improve both osteogenic and angiogenic outcomes and provide a promising strategy for bone tissue engineering and osteanagenesis.
OBJECTIVE:To summarize recent researches and related progresses in coculture of human umbilical vein endothelial cel s and bone marrow mesenchymal stem cel s.
METHODS:A computer-based online search of CNKI database from January 2000 to March 2012, PubMed database and Web of Knowledge database from January 1980 to March 2012, was performed with the keywords of“human umbilical vein endothelial cel s, bone mesenchymal stem cel s, coculture, tissue engineering”both in Chinese and English. A total of 135 articles were screened out, 103 of them were excluded due to unrelated study objective and repeated contents, and final y 32 articles were involved in further analysis.
RESULTS AND CONCLUSION:At present, studies on coculture of bone marrow mesenchymal stem cel s and human umbilical vein endothelial cel s mainly focus on mimicking in vivo environments, the interactions between cel s, and the influence of different cel ratios and culture media. Most of these researches play important roles in bone tissue engineering and bone regeneration therapy, but the mechanism of action and concrete regulation in
vivo between bone marrow mesenchymal stem cel s and human umbilical vein endothelial cel s stil need further research and analysis.

OBJECTIVE:To optimize extraction technology of saponins from Paris polyphylla. METHODS:Using paris sapo-nin Ⅰ,paris saponin Ⅱand Paridis total saponin as dependent variables,using ethanol volume fraction,extraction time and sol-vent amount as independent variables,through multiple linear regression and binomial fitting,the extraction technology was opti-mized with response surface method and predicted. RESULTS:The optimized extraction technology of saponins from P. polyphylla was as follows as 10-fold of 80% ethanol,2 times reflux extraction,100 min each time. Under the extraction technology,the ex-traction rates of paris saponinⅠwere 85.4%,82.7% and 87.1%;those of paris saponinⅡwere 85.9%,81.3% and 83.6%;and those of Paridis total saponin were 89.5%,92.1% and 90.3%(all RSD<2.0%). Measured value was 0.964 9,predicted value was 0.986 0 and deviation rate was 2.14%. CONCLUSIONS:The central composite design-response surface method is simple and reliable for the optimization of extraction technology of saponins from P. polyphylla.

Objective: To explore the influence of early partial body weight support treadmill training (PBWSTT) on motor function of lower extremities, walking ability and activities of daily living (ADL) in patients with hemiplegia after stroke. Methods: One hundred twenty-eight patients with stable stroke (hemiplegic limbs Brunstrom's scale ≥ II) within 3 months after the onset were enrolled in the study. They were randomly allocated into PBWSTT group (n = 67) and control group (n = 61). Conventional treatment techniques were used in rehabilitation training in the control group, and the PBWSTT group received PBWSTT in addition to the same training in the control group. The Functional Ambulation Category (FAC) scale, Fugl-Meyer Assessment (FMA) scale and the Functional Independence Measurement (FIM) scale were used to assess the walking ability, motor function of lower extremities, and ADL 4 weeks before and after the treatment in both groups. Results: The FAC, FMA and FIM scores were 0.42 ± 0.23, 17.1 ± 5.2 and 35.3 ± 3.8, respectively before the treatment; and they were 2.5 ± 1.3 23.6 ± 7.3 and 67.9 ± 9.2, respectively after the treatment in the control group, there were significant difference between them (P < 0.05). The FAC, FMA and FIM scores were 0.36 ± 0.13, 16.7 ± 6.2 and 35.1 ± 3.4, respectively before the treatment; and they were 3.8 ± 1.4, 39.3 ± 7.6, and 90.1 ± 10.0, respectively after the treatment in the PBWSTT group. There were significant differences before and after the treatment (P < 0.01). The FAC score versus FMA score in both groups were P < 0.01, and the FIM score was P < 0.05 after the treatment. The patients who could walk independently were 42.6% and 55.7% (P < 0.05) in the control group, and were 32.8% and 83.5% (P < 0.01) in the PBWSTT group before and after the treatment. There were significant differences between the control group and the PBWSTT group after the treatment (P < 0.05). Conclusion: On the basis of conventional rehabilitation training, early PBWSTT may improve the motor function of lower extremities, walking ability, and ADL more significantly in stroke patients with hemiplegia.

Female ; Humans ; Male ; Mandibulofacial Dysostosis ; genetics ; Pedigree ; Young Adult

It has been found by Ju('81)that HRP could be anterogradely transported forlong distances.Based on this fact the rate of anterograde transport was investigatedin the present study,and,at the same time,the rate of retrograde transport and thetime of disappearance of HRP from the labeled sites were observed.Twenty tworabbits,weighing 2～2.2 kg,were used.The HRP was injected into the lower lumbarspinal cord unilaterally.The animals were sacrificed after 12 hours to 7 days andwere processed with benzidine and o-dianisidine.The anterogade labeling in the dorsalaccessory olivary nucleus and the retrograde labeling in the red nucleus and thenucleus raphe pallidus were chosen for study.It was quite unexpected to find that the anterograde and retrograde labelingsparalled each other in time as well as in intensity.They appeared at the same timeand waxed and waned practically in full accord.Labeling of the brainstem firstappeared at 18 hour's survival,reached its peak at the 2nd day,and then graduallyfaded away till at the 7th day when only traces of labeling remained.In the casewhich showed the earliest labeling the distance between the injection site and theobex,which was at about the same level as the most densely labeled parts of thedorsal accessory olivary nucleus and nucleus raphe pallidus,was measured to be 265mm and the rate of transport,the same for anterograde and retrograde transports,was calculated to be more than 350mm per day.Counting from the time of theearliest labeling,HRP was found to remain at the labeled site for as long as 5～6 days.The period of net accumulation of HRP at the labeled sites was about 1 day.Thus,the best survival period would be roughly the time required for HRP to travel thefiber tract plus 1 day.Neurons of the red nucleus and the nucleus raphe pallidus differ greatly intheir morphology,physiology as well as biochemistry.The results in them however,were similar.This,in connection with observations on other nuclei,both anterogr-adely and retrogradely labeled,suggests that the rate of transport and the duration ofpreservation of HRP in the central nervous system are basically the same in differentneuronal systems.

HRP was injected into the right side of the cervical or lumbar enlargement ormidthoracic segment of adult cats.Retrogradely labeled cells in the red nucleuswere examined and their somatotopic localization was determined.1.The Rubro-spinal projection was mainly crossed with obvious somatotopiclocalization.The dorsal medial part of the red nucleus projected to the cervicalenlargement.The ventral lateral part of the red nucleus projected to the lumbarenlargement.The neurons between these two parts projected to the thoracic cord.2.In cervical and lumbar injected cases a few labeled cells were found also inthe ipsilateral red nucleus,suggesting a minor ipsilateral rubrospinal projection exist-ed.No ipsilateral labeling was found in the thoracic injection cases.3.The rubrospinal tract could at least project to as low as L 6 segment of thespinal cord.

The somatotopical projection from the lateral vestibular nucleus to differentlevels of the spinal cord in the cat was studied with the horseradish peroxidasemethod.It was found that in the lateral vestibular nucleus the cells projecting tothe cervical and lumbar segments of the cord were distributed over the entire ros-trocaudal extent,each with an area of concentration.The caudal and middle thirdsof the nucleus,especially its dorsolateral part,projected to the lumbar cord.Thecells projecting to the cervical cord were concentrated at the rostral-middle thirds ofthe nucleus and those to the thoracic cord were concentrated at the dorsal part ofthe caudal-middle thirds.The area projecting to the thoracic levels seemed tocoincide with that projecting to the lumbar levels.The cells projecting to thelumbar cord were largest in number,those to the cervical cord less and those to thethoracic cord least.

To improve the anesthesia skills of younger anesthesiologists ,department of anes-thesiology of the Affiliated Hospital of Yangzhou University formed the tutorial system based on tutori-al team. Through enhancing the selection of tutors,establishing of tutorial team and conducting clini-cal skill guidance and scientific research ability training,department of anesthesiology aimed to devel-op personal quality,clinical and scientific research ability of younger anesthesiologists in the process of clinical completion and application of scientific research subject.

The synchronous fluorescence spectra of myoglobin were studies for the first time. The fluorescence peaks observed in the spectra were assigned. When the wavelength interval (Δλ) is 80 nm, the main peak at 335 nm is originated from the tryptophan residues in the myoglobin molecule. When Δλis 20 mn, the peak at 308 nm is mainly due to the tyrosine residues in the myoglobin molecule and in a small part due to the tryptophan residues.Two peaks at 322 and 596 nm were observed in the spectrum of myoglobin for Δλ = 40 nm. The peak at 322 nm is due to both tyrosine and tryptophan residues. The peak at 596 nm is attributed to the heme group in the myoglobin molecule .

HRP was injected into the cervical (3 cases) or lumbar (2 cases) spinal cord unilaterally in 5 adult cats. Labeled cells were discovered in the hypoth alamus and nearby areas. There was no obvious difference in labeling between cervical and lumbar injection cases. Labeled cells were found bilaterally with ipsilateral preponderance.he paraventricular nucleus was most heavily labeled; the posterior and lateral hypothalamic areas were less. A few labeled cells were found in the dorsal hypothalamic area and the supramamillary nucleus. Forel's area was also weakly labeled and occasional cells were found in the subthalamic nucleus and zona incerta.We were unable to find labeled cells in the dorsomedial nucleus. Labeling of the supramamillary nucleus, which was found in this sutdy, has not been mentioned in the literature available to us.