Objective To investigate the role of NF-κB activation in the inflammatory mechanism of chronic obstructive pulmonary disease (COPD).Methods Monocyte were collected from patients with COPD and were cultured,and stimulated with lipopolysaccharide (LPS) ; NF-κB p65 activation was measured by immunohistochemistry; SOD ,MDA and IL-8 and lung function were determined synchronously.Results The NF-κB p65 was induced by LPS in monocyte in all subjects, but it was most markedly done in COPD patients with exacerbateions; There was positive correlation between the NF-κB p65 activation of monocytes and levels of IL-8 and MDA in circulation, but it was negative correlation to SOD.Conclusion NF-κB plays a vital role in regulating product of IL-8 in monocyte in COPD.

Objective To investigate the clinical distribution and epidemiological feature of nine respiratory pathogens of acute respiratory tract infection patients in the northern area of Henan Province,in order to provide reference for clinical effective prevention and treatment of acute respiratory tract infection.Methods The clinical distribution and epidemiological feature of nine respiratory pathogens were analyzed by a retrospective review of 11 135 serum samples which were detected by indirect immunofluorescence method from December 2014 to November 2016.Results There were 2 630 cases with nine respiratory pathogens infection,the infection rate was 23.62％.The main four respiratory pathogens were mycoplasma pneumonia (16.63 ％),influenza B virus (2.41％),parainfluenza virus (2.29 ％),adenovirus (0.79％).The nine respiratory pathogens infection rate of male and female was 21.60％ (1 516/7 020) and 27.07 ％ (1 114/4 115),respectively.The nine respiratory pathogens infection rate of ＜ 1 year group,1-5 years group,6-14 years group,15-30 years group,31-50 years group and ＞ 50 years group was 8.91％ (330/3 702),31.12％ (1 424/4 576),37.36％ (634/1 697),27.10％ (90/332),24.64％ (69/280),15.13 ％ (83/548),respectively.The nine respiratory pathogens infection rate of ＜ 1 year group was lower than that of the other five groups (P ＜ 0.05).The nine respiratory pathogens infection rate of spring group,summer group,autumn group and winter group was 24.66％ (591/2 396) 、23.86％ (631/2 645) 、31.09％ (748/2 406)、17.90％ (660/3 688),respectively.The nine respiratory pathogens infection rate of autumn group was higher than that of the other three groups (P ＜ 0.05).There were 253 patients with polyinfection which all were amphimorphic polyinfection in the 2 630 patients with respiratory pathogens infection,the polyinfection rate was 2.27％.Conclusions Respiratory pathogen infection rate was different because of different gender,different age and different season.The virus was the main pathogen of acute respiratory tract infection.Because of multiplicity of infection and seasonal distribution of pathogens,the prevention of the epidemic should be strengthened in spring and autumn season especially.

Objective To explore the structure changes of white matter of the patients with schizophrenia episode in early adulthood by diffusion tensor imaging(DTI) and in order to provide a structural neuroimaging basis for understanding the pathology of schizophrenia.Methods Twenty-six patients with schizophrenia episode in early adulthood in the Second Affiliated Hospital of Xinxiang Medical University from July 2012 to March 2014 were selected as study group and twenty-eight healthy subjects whose age,sex,education were matched with study group were selected as control group.All subjects received structural magnetic resonance imaging and DTI scans.The fractional anisotropy (FA) and the mean diffusivity (MD) of the white matter of the same encephalic region were compared between the two groups by voxel-based analyses.Results The FA values of the right anterior cingulate gyrusthe,genu of corpus callosum,the right limb of internal capsule,the bilateral external capsule,the bilateral posterior of coronal radiate,the right anterior coronal radiate of patients in the study group were significantly lower than those in the control group (P ＜ 0.05).The MD values of the bilateral limbs of internal capsule,the right cingulate gyrus,left superior longitudinal tract,corpus callosum and right anterior coronal radiate of patients in the study group were significantly higher than those in the control group (P ＜ 0.05).Conclusion Schizophrenia patients who episode in early adulthood exist widespread microstructural damage of white matter.These changes may be related to the pathological change of schizophrenia.

Objective To investigate the therapeutic effect of all-trans retinoic acid (ATRA) for lupus nephritis(LN) in mice.Methods Eighteen MRL/lpr mice were randomly divided into control group,cyclophosphamide treatment group and ATRA treatment group,with 6 rats in each group.The mice in control group were administered saline (10 mg· kg-1 · d-1) by intragastric administration for 8 weeks;the mice in ATRA treatment group were administered ATRA (10 mg · kg-1 · d-1) by intragastric administration for 8 weeks;the mice in cyclophosphamide treatment group were given cyclophosphamide (100 mg · kg-1 · d-1) by intraperitoneal injection for 2 days.Blood samples and kidney specimens were collected at the end of the experiment.The levels of blood urea nitrogen (BUN),serum creatinine (Scr) and urine creatinine (Ucr) were detected by full automatic biochemical analyzer;the changes of renal tissue structure was observed by periodic acid schiff;the expression of transforming growth factor-β1 (TGF-β1) and monocyte chemotactic protein-1 (MCP-1) was detected by immunohistochemistry.Results The levels of Scr and BUN of mice in ATRA treatment group and cyclophosphamide treatment group were significantly lower than those in the control group,and the Ucr level was significantly higher than that in the control group (P ＜ 0.01);the BUN level of mice in ATRA treatment group was significantly higher than that in the cyclophosphamide treatment group,but the Ucr level was significantly lower than that in the cyclophosphamide treatment group (P ＜ 0.01);there was no statistic difference in the Scr level of mice between the ATRA treatment group and cyclophosphamide treatment group (P ＞ 0.05).In control group,the glomerular basement nembrane and renal tubular basement membrane of mice was thicken,the mesangial of mice was proliferated;the thicken glomerular basement membrane,renal tubular basement membrane and proliferated mesangial were also seen in ATRA treatment group and cyclophosphamide treatment group which was lighten than control group,and cyclophosphamide treatment group was lighten than ATRA treatment group.The expression of MCP-1,TGF-β1 of mice in cyclophosphamide treatment group and ATRA treatment group were significantly lower than those in the control group (P ＜ 0.05);the expression of MCP-1,TGF-β1 of mice in ATRA treatment group were significantly higher than those in cyclophosphamide treatment group(P ＜ 0.05).Conclusion Both cyclophosphamide and ATRA can reduce the levels of Scr and BUN,lighten the pathological changes of renal and decrease the expression of TGF-β1 and MCP-1 in LN mice;but the effect of cyclophosphamide is better than ATRA.

Objective To investigate the effect of Xingnao Jieyu Decoction on the function of Notch/Hes signaling pathway in hippocampus of rats with post-stroke depression (PSD).Methods A total of 80 healthy male specific pathogen-free Wistar rats were randomly divided into control group,model group,prozac group and Xingnao Jieyu Decoction group;20 rats in each group.The rats in the model group,prozac group and Xingnao Jieyu Decoction group were used to prepare the cerebral ischemia model by middle cerebral artery occlusion method,and then the rats were used to prepare the depressive model by a variety of chronic unpredictable stimuli.The rats in the control group did not have any intervention.After the preparation of PSD model,the rats in the control group and model group were given saline 2.5 mL by intragastric administration,the rats in prozac group were given prozac 2.5 mL (1.8 mg · kg-1) by intragastric administration,the rats in Xingnao Jieyu Decoction group were given Xingnao Jieyu Decoction 2.5 mL (150.0 mg · kg-1) by intragastric administration;one times daily for 28 days.Ten rats in each group were sacrificed immediately on the nineteenth and twenty-eighth day of drug intervention,and the hippocampus tissues were isolated.The expression of Notch and Hes mRNA and protein in hippocampus were detected by real-time quantitative polymerase chain reaction and Western blot method.The open probability,current amplitude,opening time and closing time of Notch signaling pathway were detected by patch clamp technique.Results On the nineteenth and twenty-eighth day of drug intervention,the expression of Notch and Hes protein and mRNA in hippocampus of rats in the model group,prozac group and Xingnao Jieyu Decoction group was significantly higher than that in the control group (P ＜ 0.05);the expression of Notch and Hes protein and mRNA in hippocampus of rats in the prozac group and Xingnao Jieyu Decoction group was significantly higher than that in the model group (P ＜ 0.05);the expressions of Notch and Hes protein and mRNA in hippocampus of rats in the Xingnao Jieyu Decoction group was significantly higher than that in the prozac group (P ＜ 0.05).There was no significant difference in Notch and Hes protein and mRNA expression in hippocampus of rats between the nineteenth and twenty-eighth day of drug intervention (P ＞ 0.05).The expression of Notch and Hes protein and mRNA in hippocampus of rats on the twenty-eighth day was significantly higher than that on the nineteenth day in the model group,prozac group and Xingnao Jieyu Decoction group (P ＜ 0.05).Compared with the control group,the open probability and current amplitude of Notch signaling pathway in hippocampus of rats increased significantly,the opening time prolonged significantly,and the closing time shortened significantly in the model group,prozac group and Xingnao Jieyu Decoction group on the nineteenth and twenty-eighth day of drug intervention (P ＜ 0.05).Compared with the model group,the open probability and current amplitude of Notch signaling pathway in hippocampus of rats increased significantly,the opening time prolonged significantly,and the closing time shortened significantly in the prozac group and Xingnao Jieyu Decoction group on the nineteenth and twenty-eighth day of drug intervention (P ＜ 0.05).Compared with the prozac group,the open probability and current amplitude of Notch signaling pathway in hippocampus of rats increased significantly,the opening time prolonged significantly,and the closing time shortened significantly in the Xingnao Jieyu Decoction group on the nineteenth and twenty-eighth day of drug intervention (P ＜ 0.05).There was no significant difference in the open probability,current amplitude,opening time and closing time of Notch signaling pathway between the nineteenth and twenty-eighth day of drug intervention in the control group(P ＞ 0.05).Compared with the nineteenth day of drug intervention,the open probability and current amplitude of Notch signaling pathway in hippocampus of rats increased significantly,the opening time prolonged significantly,and the closing time shortened significantly on the twenty-eighth day of drug intervention in the model group,prozac group and Xingnao Jieyu Decoction group (P ＜ 0.05).Conclusion Xingnao Jieyu Decoction can mediate the activation of Notch / Hes signaling pathway in hippocanpus of PSD rats,thus effectively promote the recovery of neurological function.Notch/Hes signaling pathway is expected to be an important target for drug action.

Objective To observe the effect of nuclear factor-κB (NF-κB) signaling pathway on the expression of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) in nasal polyp cells under hypoxic cultivation,and to investigate the relationship between NF-κB signaling pathway and the development of nasal polyp.Methods The nasal polyp and inferior turbinate tissue specimens were collected in the First Affiliated Hospital of Jiamusi University from January 2012 to December 2014.The nasal polyp and inferior turbinate tissues were taken to obtain nasal polyp cells and inferior turbinate cells,then the cells were cultured in primary culture,and the cells were cultured under hypoxia when they grew to 90％.When the cells were cultured in vitro to 90％,the NF-κB inhibitor BAY11-7082 was added (inhibitor intervention group),the other cells without inhibitor were used as controls (no inhibitor group),then the cells in the two groups were cultured under hypoxia.The cells were collected when they were cultured for 0,3,6 and 9 hours,respectively;and the expression of HIF-1α,VEGF and NF-κB p65 protein in the cells were detected by Western blot.Results Compared with 0 hour,the expression of HIF-1α,VEGF and NF-κB p65 protein in nasal polyp cells increased significantly after 3,6 and 9 hours of hypoxic cultivation (P ＜ 0.05);however,the expression of HIF-1α,VEGF and NF-κB p65 protein in inferior turbinate cells was not statistically significant (P ＞ 0.05).The expression of HIF-1α,VEGF and NF-κB p65 protein in nasal polyposis cells after 6 hours of hypoxic cultivation was significantly higher than that after 3 and 9 hours of hypoxic cultivation (P ＜ 0.05);but there was no significant difference in the expression of HIF-1α,VEGF and NF-κB p65 protein in nasal polyp cells between 3 and 9 hours of hypoxic cultivation (P ＞ 0.05).Compared with 0 hour,the expression of HIF-1α and VEGF protein in nasal polyp cells of no inhibitor group increased significantly after 3,6 and 9 hours of hypoxic cultivation (P ＜ 0.05);and the expression of HIF-1α and VEGF protein in nasal polyp cells after 6 hours of hypoxic cultivation was significantly higher than that after 3 and 9 hours of hypoxic cultivation in no inhibitor group (P ＜ 0.05).But there was no significant difference in the expression of HIF-1α and VEGF protein in nasal polyp cells of no inhibitor group between 3 and 9 hours of hypoxic cultivation (P ＞ 0.05).There was no significant difference in the expression of HIF-1 α and VEGF protein in nasal polyp cells of the inhibitor intervention group among 0,3,6 and 9 hours of hypoxic cultivation (P ＞ 0.05).There was no significant difference in the expression of HIF-1α and VEGF protein in nasal polyp cells between no inhibitor group and inhibitor intervention group at 0 hour of hypoxic cultivation (P ＞0.05).The expression of HIF-1α and VEGF protein in nasal polyp cells of inhibitor intervention group was significantly lower than that of no inhibitor group after 3,6 and 9 hours of hypoxic cultivation (P ＜ 0.05).Conclusion The expression of HIF-1α,VEGF and NF-κB p65 protein increased in nasal polyp cells under hypoxia condition.NF-κB signaling pathway may mediate hypoxia-induced HIF-1α and VEGF protein expression,and participate in the occurrence and development of nasal polyp.

Objective To investigate the clinical distribution and resistance change of pseudomonas aeruginosa (PAE) in the four hospitals of Henan province during 2012-2015,so as to provide guidance for reasonable clinical use of antibiotics.Methods By means of disk diffusion method (K-B method) or automatic instrument,the drug susceptibility testing was performed for the clinical isolated strain which collected from the 371st Central Hospital of PLA,the Third Affiliated Hospital of Xinxiang Medical University,the 150th Central Hospital of PLA and the 153rd Central Hospital of PLA during 2012-2015,then the result were interpreted according to the criteria of Clinical and Laboratory Standards Institute 2010,and the date were analyzed by using WHONET 5.6 software.Results A total of 1 796 strains of PAE had been isolated,and mainly distributed in department of critical care medicine (32.6％) and department of gastroenterology (28.7％).The specimens mainly collected from sputum (74.6％) and wound secretion (9.6％).The drug susceptibility results showed that the antibiotic sensitive rate of PAE to polymyxin B(93.5％) was the highest,followed by the piperacillin/tazobactam(78.2％) and amikacin(76.2％).Resistance rate of PAE to piperacillin(34.4％) was the highest,followed by the ticarcillin/calvulanic acid(34.1％) and aztreonam (30.7 ％).Drug-resistance of PAE to the ceftazidime,cefepime,aztreonam,amikacin,gentamicin and polymyxin B had a downward trend,respectively.Conclusion Drug-resistance of the PAE to the most commonly used antimicrobial has a downward trend.However,the drug-resistance situation of PAE is very serious and the resistance rates still keep the high level during 2012-2015.It is necessary to investigate the clinical distribution and resistance of PAE,so as to provide guidance for reasonable clinical use of antibiotics.

Objective To understand the present internet addition of Chinese college students and to explore the relationship between the types of internet addition and learning burnout,and to provide reference and theoretical basis for domestic research on internet addition.Methods Two-stage stratified sampling was used to select 1 485 students in Grade one to Grade three from seven colleges and universities in Henan province including Xinxiang Medical University,Sanquan College of Xinxiang Medical University,Xinxiang University,Henan Institute of Science and Technology,Henan Normal University,Henan University of Technology,Engineering College of Zhengzhou University.The university students questionnaire of internet addiction types and the leaning burnout scale were used and the results were analyzed.The students with internet addiction were divided into high score group and low score group according to result of questionnaire of intemet addiction types,and the difference of learning burnout between them was analyzed.Results In the types of internet addiction,the leaning burnout scores and the score of each dimensional scores in the high score group were significantly higher than those in the low score group (P ＜ 0.01).The network game addiction scores was positively related to depression,misconduct and learning burnout(P ＜ 0.05,P ＜ 0.01);questionnaire of network information scores and depression had positive correlation (P ＜ 0.05).Conclusion The tendency of network information addiction is higher,the manifestation of depression is more obvious.The more obvious tendency of network information addiction,network relationship addiction,network information addition,which more easily leads to learning burnout.

Objective To observe the changes of the levels of serum hemoglobin (HGB),vascular endothelial growth factor (VEGF),carcinoembryonic antigen (CEA),carbohydrate antigen 19-9 (CA19-9) and carbohydrate antigen 125 (CA125) in patients with gastric cancer before and after chemotherapy;and to investigate the correlation between the changes of the levels of the serum indexes and the effect of chemotherapy.Methods A total of 100 patients with gastric cancer were selected from June 2015 to June 2016 in the Central Hospital of Tongchuan Mining Bureau.All patients were treated with the primary chemotherapy regimen with fluorouracil and paclitaxel.The levels of serum HGB,VEGF,CEA,CA19-9 and CA125 were detected before and after one cycle of chemotherapy.The curative effect was evaluated after two cycles of chemotherapy.Results Among the 100 patients after two cycles of chemotherapy,there were 25 cases with partial remission (effective group),37 cases with stable disease(stable disease group) and 38 cases with progressive disease (progressive disease group).There was no significant differences in serum HGB level of the 100 patients before and after chemotherapy (P ＞ 0.05),but the levels of serum VEGF,CEA,CA19-9 and CA125 after chemotherapy were significantly lower than those before chemotherapy (P ＜0.05).There was no significant difference in serum HGB,VEGF,CEA,CA19-9 and CA125 levels among the three groups before chemotherapy (P ＞ 0.05).The levels of serum HGB,VEGF,CEA,CA19-9 and CA125 after chemotherapy were significantly lower than those before chemotherapy in the effective group (P ＜ 0.05).There was no significant difference in the levels of serum HGB,VEGF,CEA,CA19-9 and CA125 before and after chemotherapy in the stable disease group (P ＞ 0.05).There was no significant difference in the levels of serum HGB,VEGF,CEA and CA19-9 before and after chemotherapy in the progressive disease group (P ＞ 0.05);but the level of serum CA125 after chemotherapy was significantly higher than that before chemotherapy (P ＜ 0.05).The levels of serum HGB,VEGF,CEA,CA19-9 and CA125 in the effective group were significantly lower than those in the stable disease group and the progressive disease group after chemotherapy (P ＜ 0.05).There was no significant difference in serum HGB,VEGF and CA19-9 levels between the stable disease group and the progressive disease group (P ＞ 0.05);but the levels of serum CEA and CA125 in stable disease group were significantly lower than those in the progressive disease group after chemotherapy (P ＜ 0.05).Multiple regression analysis showed that the changes of the levels of serum CEA,CA19-9,CA125 and VEGF were significantly correlated with the effect of chemotherapy (P ＜ 0.05);the sequence of the correlation was CEA,CA19-9,CA125 and VEGF.There was no significant correlation between the serum HGB level and the effect of chemotherapy (P ＞0.05).Conclusion The changes of serum CEA,CA19-9,CA125 and VEGF levels were correlated with the effect of chemotherapy in patients with gastric cancer.Detecting serum CEA,CA19-9,CA125 and VEGF levels is helpful to predict the effect of chemotherapy.

Objective To compare the clinical effect of microwave ablation and surgical resection in the treatment of primary liver cancer.Methods A total of 76 patients with primary liver cancer were selected from May 2010 to June 2012 in the Fourth People's Hospital of Shangqiu City.The patients were divided into microwave ablation group (n =46) and hepatectomy group (n =30) according to the treatment.The operation time,blood loss,hospitalization time,the levels of serum alanine aminotransferase (ALT) and total bilirubin (TBIL) before and after operation,and postoperative complications were compared between the two groups.All patients were followed up for five years,and the disease-free survival rates of 1-,3-and 5-year were observed and compared between the two groups.Results The operation time and hospitalization time in the microwave ablation group were significantly shorter than those in the hepatectomy group (P ＜ 0.05),and the intraoperative blood loss in the microwave ablation group was significantly less than that in the hepatectomy group (P ＜ 0.05).There was no significant difference in the levels of serum ALT and TBIL between the two groups before operation (P ＞ 0.05).The levels of serum ALT and TBIL at the time points of one day and one week after operation were significantly higher than those before operation in the hepatectomy group (P ＜ 0.05).There was no significant differences in the levels of serum ALT and TBIL in the microwave ablation group between before operation and one day or one week after operation (P ＞ 0.05).The levels of serum ALT and TBIL in the microwave ablation group were significantly lower than those in the hepatectomy group at the time points of one day and one week after operation (P ＜ 0.05).The incidence of postoperative complication in the microwave ablation group and the hepatectomy group was 4.35％ (2/46) and 23.33％ (7/30) respectively,the incidence of postoperative complication in the microwave ablation group was significantly lower than that in the hepatectomy group (x2 =6.269,P ＜ 0.05).The disease-free survival rate of 1-,3-and 5-year was 67.39％ (31/46),39.13％ (18/46) and 28.26％ (13/46) respectively;and the tumor recurrence rate was 54.35％ (25/46) in the microwave ablation group.The disease-free survival rate of 1-,3-and 5-year was 70.00％ (21/30),50.00％ (15/30) and 33.33％ (10/30) respectively;and the tumor recurrence rate was 50.00％ (15/30) in the hepatectomy group.There was no significant difference in the disease-free survival rate of 1-,3-and 5-year and tumor recurrence rate between the two groups (P ＞ 0.05).Conclusion Microwave ablation therapy in the treatment of primary liver cancer has less trauma,less intraoperative blood loss,shorter hospitalization time,the lower incidence of postoperative complication,high safety and feasibility.Microwave ablation can better protect the liver function of patients.Microwave ablation and hepatectomy for primary liver cancer can achieve the similar disease-free survival rate and tumor recurrence rate.