From Jan.2002 to Nov.2003, umbilical cord blood was collected, screened and processed at HCM City Blood Transfusion and Hematology Hospital for preparing to transplant. The Tokyo Umbilical Cord Blood Bank protocol of collecting, screening, processing and freezing were abided strictly, therefore, the obtained results were consistent with international literature. A stable procedure was established for the proper processus of UCB collection, screening and processing to meet the need of management of hereditary and malignant pathologies in Viet Nam
Fetal Blood ; Blood ; Cord Blood Stem Cell Transplantation

From Aug-2000 to Aug-2003 at HCM City Blood Transfusion and Hematology hospital, 31 cases of endoscopic splenectomy were studied to evaluate the effect of this surgery to treat chronical immuno-thrombocytopenic purpurea once corticotherapy and immunosupressive therapy failed. Results showed that one year after splenectomy, 64,52 – 76,92%, and 2 years after, 50% of cases had had completely positive response. There was absolute failure in 38,7% of cases within 3 first months, but from the 4th month, there was no relapse case. A half number of patients with failed splenectomy had had quick response to corticotherapy more.The duration of treatment was short, endoscopic splenectomy was safe. There was no death. No case of blood transfusion was needed. There were no complications
Splenectomy ; Therapeutics ; Purpura, Thrombocytopenic

From Nov.2001 to July 2003, the analysis of chromosomes of 92 patients with CML evealed the metaphase figure of chromosomes of bone marrow cell which will be obtained easier than blood, but the blood is better Philadelphia chromosome found in 91,25% of cases of success.There is no significant difference between male and female subjects as between various age groups
Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; Philadelphia Chromosome ; Bone Marrow Cells

From Jul.1995 to Oct.2003, at HCM City Blood Transfusion and Hematology Hospital, Hickman catheter was inserted on 41 patients with AML,ALL,BMT,Thalassemia. Main complications were bacterial infections, which accounted for 14,9%. In addition, some events occured accidentally such as chocking up (7,3%), breaking (4,8)% of the cuff in removal of catheter
Catheterization ; Bacterial Infections ; Device Removal

From Apr. 2001 to Nov. 2003, at HCM City Blood Transfusion and Hematology Hospital, study was performed to evaluate preliminarily the quality of stem cell preparation producted from umbilical blood cells after processed with CD34+ cells count , flown cytometry and with stem cell culture on methylcellulose medium. Results showed :the average CD34 stem cell count after processing in umbilical cord blood bag 1,94+/-1,05x1000 000 In 1ml cell culture : BFU- E:0,25x 1000 /ml, CFU-GM:6,75x 1000 /ml, CFU-Mix:8,55x1000 /ml
Cord Blood Stem Cell Transplantation ; Blood ; Cells

A total of 200 pathogenic strains of Escherichia coli collected on diarrhoea patients at the hospital BachMai, DongDa and DongAnh in Hanoi were studied.By multiplex PCR assay, genes of various toxicity levels were determined on 5 pathogenic E coli: 2 EPEC, 5 ETEC , 8 EAEC. There were 15 strains in which, pathogenic genes of E.coli causing diarrhoea which accounted for 7,5%.In comparing the multiplex PCR technique and simplex PCR technique, results were the same
Escherichia coli ; Diagnosis ; Polymerase Chain Reaction

In the year 2002, an interview was conducted on 180 households, where women account for 67,2%, men 32,8%, mean age is 47 years old. Among the interviewers, 100% had known of the disease; 88,3% had known of the symptoms, 95% had known of the transmission route, 94% had thougth that the disease could be prevented, 60,9% had believed in health authorities in behaving as them, 51,5% had complied with health workers, 60,9% had believed in radio and TV. There was a relation of statistic significance between the knowledge and the practice of use of chemical mosquito killers, mosquito net, mosquito killing fish, the storage of water at home ,as well as the environment sanitation. In high educated people, knowledge and practice of prevent of hemorrhagic dengue/dengue fever were better than in low educated ones
Knowledge ; Dengue/prevention & control ; Epidemiology

In the year 2003, for Hantaan virus, a serological surveillance ELISA method was performed on 155 serum samples collected from the workers of Haiphong port.26/155 samples were positive or in suspect with rodent sera. The samples submitted to 2nd examen by indirect fluoresceine immuno-assay showed that 3/26 samples were positive 3 positive samples were undertaken further studies by WB technique with specific SEO antigenes ,which confirmed anti-hantaan virus (IgG) and nuclecapsid protein (NP) of51 kD weigt. Results showed that Hantaan virus was found among Haiphong port workers
Hantavirus ; Serum ; Enzyme-Linked Immunosorbent Assay

In remote, mountainous, sea island and frontier areas, there are various shortcomings in health service domain because of the difficulties to access, the under developed transportation and communication, the thinly populated distribution, the low educated level of the people, the lacking and low qualifying of medical personel staff. Some solutions are recommended: To organize the forms of civil and military in combination, to establish intercommunal polyclinic consultation rooms, to make actively the politics of training, attracting and well treated medical manpowers, to enlarge the health insurance with multiform and flexible models
Health ; Health Services ; Ediucation ; Medical Staffs

Serum samples from patients with Dengue hemorrhagic fever were collected at the hospitals of Hanoi,Namdinh,Thanhhoa and NgheAn in 1995-2001 year period were investigated.The number of suspected patients with Dengue hemorrhagic fever was varied during the years, the highest in the year 1995, 1998, then decreased progressively to 2001. In 2 years of 1995-1996 period and 1998-2001 period 4 types of epidemiologic virus were found. The highest number of isolated samples was 54 in the year 1996, then1998. The endemic types varied obviously by years
Dengue ; Epidemiology ; Serum