Objective:To observe the effect of two different apical surgery timing. Methods:68 patients with periapical lesion were divided into 2 groups. 30 patients( control group) were operated by apical surgery at least 1-2 months after root canal therapy( RCT) , while 38 patients(experimental group) were operated immediately after RCT. The patients were followed up 3, 6 and 12 months after apical surgery. Results:The curative effect analysed with the age, sex and tooth position showed no statistical difference between 2 groups(P>0. 05). Conclusion:Immediate and delayed apical surgery apical surger after RCT are similarly effective.

Patients underwent impacted mandibular wisdom tooth removal were randomly divided into 3 groups before operation.Patients in group A (n =41) were given pericoronal rinsing with 3 ％ H2O2 and saline before operation and irrigation once with povidone-iodine after tooth removal.Those in group B (n =43) were given irrigation with saline before and after tooth removal.47 patients in group C were given no irrigation as the controls.There was no significant difference in postoperative pain 3 days after operation among the 3 groups.Significant difference was found in postoperative pain 7 days after operation and mouth-opening 3 and 7 days after operation.There was significant difference in PoSSe scores among 3 groups but no difference between group A and B.It could be concluded that irrigation in pre-and post-operation can reduce the influence of teeth removal on life quality of the patients.

RGS16 protein expression in 84 cases of oral squamous cell carcinoma(OSCC) was examined by immunohistochemical staining method.RGS16 protein expression was positive in 78 out of the 84 cases.The expression was associated with the clinical stage of OSCC(P ＜ 0.05),but not with patient's age,gender,degree of differentiation and lymph node metastasis(P ＞ 0.05).

Objective:To evaluate skeletal,dentoalveolar and soft tissue profile changes by activator therapy in patients with different skeletal patterns of Class Ⅱ1 malocclusions.Methods:22 subjects(10 girls,12 boys,mean age 11.5 ±0.67 years) in the mixed or early permanent dentition,were included and divided into low angle(n =15) and average angle(n =7) groups on the basis of skeletal pattern.All patients were treated with a traditional activator.The skeletal,dentoalveolar and soft tissue profile changes were compared on lateral cephalograms before and after treatment.Statistical analysis was performed with t-test of SPSS 13.0 at a level of significance of P ＜ 0.05.Results:Activator treatment in these growing patients resulted in a correction of the skeletal Class Ⅱ relationship (decrease of ANB,Wits and NA-Pg),an advancement of the mandibular structures(increase of Co-Pg and L1-APg),and changes of the teeth(increase of L6-MP).The changes of Wits,NA-Pg and L1-APg value of low and average angle groups were 1.34° ± 1.82 ° and 3.50 ° ± 1.77°,(3.06 ± 2.00) mm and (5.80 ± 3.17) mm,(-1.16-± 1.74) mm and (-2.83 ± 1.48) mm respectively(P ＜0.05).No statistical significance was found in the soft tissue profile changes whether intra-class or inter-group comparison.Conclusion:The activator appliance is effective in treating growing patients with mandibular deficiency,and mandibular reconstruction,in patients with average angle it is more effective than in those with low angle.

Objective:To analyze the incidence,treatment and resource consumption of primary oral squamous cell carcinoma (OSCC) in Beijing inhabitants.Methods:Consecutive cases of primary OSCC of Beijing inhabitants admitted to the Stomatology Hospital of Peking University between 2009-2013 were selected from the medical record department.A retrospective analysis was performed on the clinical data which including age,sex,sites,TNM stage,habits of smoking and drinking,treatment,fees,etc.Results:415 cases were included in this analysis.The male-to-female ratio was 1.2∶ 1.Tongue was the most common affected site(41.0％).The difference of the distribution of the affected sites between male and female patients was statistically significant(P ＜0.01).96.6％ patients were diagnosed above the age of 40 years with the median age of 64(56,73).The difference of the distribution of age between male and female patients was statistically significant(P ＜ 0.01).The patients with stage Ⅲ-Ⅳ accounted for 54.9％.With the development of T stage of the primary tumor,the average hospital fee,surgery fee and hospitolization time increased correspondingly.Conclusion:Late stage OSCC accounts for over half of all OSCC patients.The consumption of medical resources increases significantly with the development of tumor,thus more preventive measures are required.

Objective:To study the differentially expressed proteins in saliva of health people and the patients with oral squamous cell carcinoma(OSCC).Methods:Saliva of 17 cases with OSCC and paired health subjects was collected,the proteins in the saliva were examined by two-dimensional gel electrophoresis (2-DE) separation,the proteins were examined by 2-DE separation,the saliva proteome dimensional electrophoresis profiles were obtained by MALDI-TOF/MS mass spectrometric identification,the information of the differentially expressed protein in OSCC group was studied by NCBI database bioinformatics analysis.Results:10 proteins differentially expressed between the 2 groups were observed by mass spectrometry.Bioinformatics analysis showed that S100A8,S100A8/S100A9 and Epidermal cytokeratin 2(EK2) were highly expressed in the saliva of OSCC cases.Conclusion:S100A8,S100A8/S100A9 and EK2 may be related to the development of OSCC.

Objective:To study the effects of Ti-TiO2 nanotubes with different diameters on the proliferation,stretching and collagen secretion of fibroblasts.Methods:TiO2 nanotubes formed at 1,5,10 and 20 V potentials served as the experimental samples and polished pure titanium served as the control.Fibroblasts was cultivated on the surface of the various samples.MTT assay was used to examine the cell proliferation.The surface morphology of the cells was observed with SEM.Collagen secrection was tested by sirius red/bitter acid staining.Results:The nanotubes prepared by 1,5,10 and 20 V were with the diameter of 15,30,50 and 100 nm respectively.At day 1,3 and 5,the cell proliferation on polished pure titanium surface was more than that on the nanotubes surfaces at the same time;at day 5,cell proliferation on 100 nm nanotubes was significantly more than that on the other nanotube surfaces (P ＜ 0.01).At day 3,fibroblasts at polished pure titanium surface stretched as typical long spindle form,while with obvious pseudopodium at nanotube surfaces.The collagen secretion of fibroblasts was highest at 100 nm nanotubes (P ＜ 0.01).Conclusion:100 nm nanotubes may have minimal negative effect on fibroblast proliferation and the greatest positive effect on the collagen secretion.

Fused teeth is defined as the union of enamel and dentin of two or more teeth germs,yielding a single tooth during tooth develop-ment.Their treatment is complex and usually need multidisciplinary approaches.This report presents a case of fused teeth treated by he-misection and discusses the clinical features and treatment options.

1 case of OSAS was treated by valuating rapid maxillary expansion,sleep monitoring indicated that the device reduced nasal re-sistance and improved respiratory function of the patient.

Biosplint splinting ribbons and flowable restorative were used to stabilize the mobile anterior teeth and replace the missed teeth with in 30 patients severe periodontitis.1 and 2 years after treatment the effective ratio was 86.7% and 83.3% respectively(P >0.05), probing depth and attchment loss were decreased(P <0.05).