Objective To explore the relationship between epstein-barr viral(EBV)load and T lymphocyte subsets distribution in peripheral blood of children with systemic lupus erythematosus(SLE)and its synergistic effect on liver injury.Methods A total of 212 children with SLE admitted to the Xinjiang Uygur Autonomous Region Children's Hospital from October 2021 to September 2023 were selected as the study subjects.The SLE disease activity index(SLEDAI)score was divided into active group(n=58)and inactive group(n=154).The clinical data and laboratory indexes of the two groups were compared.The curve relationship between EBV load and T lymphocytes was analyzed by local weighted scatterplot smoothing(LOWESS)method.According to the occurrence of liver injury,212 children with SLE were divided into two groups(n=166)without liver injury and(n=46)with liver injury.The clinical data of the two groups were compared.Multifactor Logistics regression analysis was used to screen the influencing factors of liver injury in SLE children.Restricted cubic spline(RCS)model was used to analyze the relationship between EBV load,T lymphocytes and liver injury.The unconditioned Logistic regression model was used to analyze the interaction between EBV load and T lymphocytes on liver injury.Results Compared with the inactive group,the active group had younger age(t=9.265),higher hormone dosage(t=2.369),decreased ALB,CD4+,CD4+/CD8+levels(t=4.870,3.463,5.820),and increased EBV load,CD3+,CD8+levels(t=40.830,12.719,5.048),and the differences were statistically significant(all P<0.05).LOWESS analysis showed that EBV load had a nonlinear relationship with CD3+,CD4+,CD8+and CD4+/CD8+.The proportion of children with liver injury who had a disease course of≤3 months,EBV load,CD3+and CD8+levels,and the proportion of active children were lower than those without liver injury(t/χ2=10.749～30.378);the levels of ALB,CD4+and CD4+/CD8+indexes were higher than those without live injury(t=13.561,3.117,13.204),and the differences were statistically significantly(all P<0.05).Multivariate Logistic regression analysis showed that disease duration≤3 months,EBV load,CD3+,ALB,CD4+/CD8+and active SLE were influential factors for liver injury in SLE children(Wald χ2=1.020～1.932,all P<0.05).The results of RCS model analysis showed that EBV load,CD3+and CD8+were positively correlated with liver injury in SLE children,and CD4+,CD4+/CD8+were negatively correlated with liver injury in SLE children.Interaction analysis results showed that EBV load≥450 IU/ml had a multiplicative and additive interaction with CD3+≥60%,CD4+<30%,CD8+≥37%and CD4+/CD8+<0.94,and the risk of liver injury in SLE children was higher when the two factors co-existed.Conclusion There is a nonlinear relationship between EBV load in peripheral blood of SLE children and CD3+,CD4+,CD8+and CD4+/CD8+.Peripheral blood EBV load and abnormal expression of CD3+,CD4+,CD8+and CD4+/CD8+are all factors affecting liver injury in children with SLE,and there is a multiplicative and additive interaction between the above factors.

Objective To investigate the effect of α/β hydrolase folded protein 5(ABHD5)and cellular immunity markers on the response to tirellizumab targeted therapy in patients with advanced non-small cell lung cancer(NSCLC).Methods A total of 123 patients with advanced NSCLC who received tiralizumab in Kailuan General Hospital from October 2020 to December 2023 were selected.The efficacy of the target lesions was evaluated 4 weeks after treatment,and the patients were divided into response group and non-response group according to the treatment effect.Quantitative real time polymerase chain reaction(RT-qPCR)was used to detect the relative expression of ABHD5 in lesion tissues.The expression level of ABHD5,immune indexes[CD4+T,CD8+T,CD4+T/CD8+T,regulatory T cell(Treg),T helper cell 17(Th17),Treg/Th17]and other clinical indexes were compared between the two groups.Multivariate logistic regression analysis of independent factors influencing response to tirelizumab targeted therapy.The predictive value of ABHD5,CD4+T and CD8+T in non-response to treatment of advanced NSCLC was analyzed by receiver operating characteristic(ROC).ABHD5 expression was correlated with CD4+T and CD8+T cells by Pearson correlation analysis.Results Among the 123 patients enrolled,90 responded to treatment and 33 did not respond.The expression of ABHD5(1.16±0.18)and the levels of CD4+T(31.52%±4.26%)and CD8+T(24.39%±1.87%)cells in the non-response group were lower than those in the response group(1.47±0.21,36.43%±4.08%,29.13%±2.15%),and the levels of Treg(7.24%±0.89%)and Th17(6.23%±1.10%)cells were higher than those in the response group(6.37%±0.91%,5.42%±0.66%),and the differences were statistically significant(t=4.725～11.200,all P<0.05).There were significant differences in tumor size,number of metastases and levels of carcinoembryonic antigen(CEA),albumin(ALB)and total bilirubin(TBIL)between the two groups,and the differences were statistically significant(t=2.969～6.523,all P<0.05).ABHD5 expression and CD4+T,CD8+T cell levels were independent protective factors affecting the response to tirelizumab targeted therapy in advanced NSCLC(Wald χ2=15.803,7.954,8.631,all P<0.05).ABHD5 predicted that the AUC of non-response to treatment of advanced NSCLC was 0.897,which was higher than that of 0.860 and 0.835 predicted by CD4+T and CD8+T cells,and the prediction sensitivity and specificity were 72.73%and 94.45%,respectively.ABHD5 expression was positively correlated with the levels of CD4+T and CD8+T cells(r=0.367,0.355,all P<0.05).Conclusion The response to tirelizumab targeted therapy in advanced NSCLC is significantly correlated with the expression of ABHD5 and the levels of CD4+T and CD8+T cells,and ABHD5 has high predictive value in the treatment of advanced NSCLC.

Objective To investigate the association between polymorphisms at the rs35678,rs2289274 loci of the ATPase plasma membrane Ca2+transporting 2(ATP2B2)gene and benign paroxysmal positional vertigo(BPPV).Methods 186 BPPV patients admitted to Xi'an Trade Union Hospital from January 2022 to January 2024 were selected as the BPPV group,and another 100 healthy individuals who underwent physical examination during the same period were selected as the control group.Polymerase chain reaction(PCR)was used to detect polymorphisms at the rs35678 and rs2289274 loci of the ATP2B2 gene.Allele and genotype frequencies were compared between the two groups.Unconditional Logistic regression was used to analyze the correlation with BPPV susceptibility under three genetic models(co-dominant,dominant and recessive)for the rs35678 and the rs2289274 loci.Results The distribution of genotypes at the rs35678 and rs2289274 loci of the ATP2B2 gene in the control group,and the BPPV group were all in accordance with the Hardy-Weinberg law of equilibrium(χ2=0.003～0.050,all P>0.05),and had a representativeness of the groups.Compared with the control group,the allele T frequency(56.45%vs 41.00%)and genotype TT frequency(31.87%vs 16.81%)were significantly higher in the BPPV group at the rs35678 locus,and the allele A frequency(54.30%vs 42.00%)and genotype AA frequency(29.48%vs 17.64%)at the rs2289274 locus,and the differences were statistically significant(χ2=3.936～12.290,all P<0.05).Under the codominant model(CC vs TT)for the rs35678 locus,carriers of TT genotype had an increased risk of disease compared to carriers of CC genotype(OR=1.851,95%CI:1.059～2.936);Under both the dominant model(CT+TT vs CC)and recessive model(CT+CC vs TT),the rs35678 polymorphism showed a statistically significant association with BPPV(OR=1.716,95%CI:1.074～2.936;OR=0.759,95%CI:0.517～0.837),and the differences were statistically significant(all P<0.05),reqectively.Under the codominant model(GG vs AA)for the rs2289274 locus,carriers of the AA genotype had a higher risk of disease compared to carriers of the GG genotype(OR=1.627,95%CI:1.191～2.973);in the dontinant model(AG+AA vs GG)the polymorphisms at the rs2289274 locus showed a significant association with BPPV(OR=1.941,95%CI:1.191～3.673),the differences were statistically significant(P<0.05).Conclusion The TT genotype at the rs35678 locus and the AA genotype at the rs2289274 locus of the ATP2B2 gene increase the risk of developing BPPV.

Objective To explore the association between rs750064,rs1078761 loci polymorphisms of BPI fold containing family A member 1(BPIFA1)gene and susceptibility to asthma in pediatric patients with allergic rhinitis(AR).Methods A total of 136 cases of AR admitted to Heping Hospital Affiliated to Changzhi Medical College from March 2021 to May 2024,aged 2～10 years old,were selected as the observation group,including 70 cases of asthma with AR,the control group was 66 cases with AR alone.The laboratory indicators of the children were collected,the rs750064 and rs1078761 loci of BPIFA1 gene were genotyped by MassArray System.The differences in genotype distribution and allele frequency between the two groups were compared.The correlation between rs750064 and rs1078761 loci of BPIFA1 gene and susceptibility to asthma in children with AR was analyzed by unconditional Logistic regression.Results Compared with the control group,interleukin-6(IL-6),fractional exhaled nitric oxide(FeNO)and immunoglobulin E(IgE)levels in the observation group were significantly increased,while forced vital capacity(FVC)levels were significantly decreased,with statistical significance(t=-22.648～4.879,all P<0.05).The genotypes of rs750064 and rs1078761 of BPIFA1 gene in control group and observation group were in line with Hardy-Weinberg equilibrium law(χ2=1.492～5.549,all P>0.05),indicating population representation.The distribution frequencies of allele T and genotype TT at rs750064 of BPIFA1 gene and allele A and genotype AA at rs1078761 locus in the observation group were higher than those in the control group,and the differences were statistically significant(χ2=8.251～10.273,all P<0.05).The results of unconditional Logistic regression showed that in the co-dominant model(CC vs CT)of rs750064,the risk of CC genotype carriers was lower than that of TT genotype carriers(OR=0.537,95%CI:0.276～1.804).In the dominant model(CT+CC vs TT)and the recessive model(CT+TT vs CC),rs750064 polymorphism was associated with the risk of AR with asthma(all P<0.05).In the co-dominant model(GG vs AG),the risk of rs1078761 in GG genotype carriers was lower than that in AA genotype carriers(OR=0.498,95%CI:0.176～1.205).Under the dominant model(AG+GG vs AA)and recessive model(AG+AA vs GG),rs1078761 polymorphism was associated with the risk of AR combined asthma with statistical significance(all P<0.05).After adjusting various factors,rs750064 and rs1078761 were associated with the risk of AR combined with asthma under the three genetic models,and the differences were statistically significant(all P<0.05).Conclusion The polymorphism of BPIFA1 gene is significantly related to the susceptibility of asthma in children with AR.TT carriers of rs750064 locus and AA carriers of rs1078761 locus are more likely to develop asthma in children with AR.

Objective To investigate the relationship between calcium-sensing receptor(CaSR)gene rs17251221,rs60388563 loci polymorphisms and the genetic susceptibility of breast cancer(BC).Methods A total of 122 patients with breast cancer admitted to Hengshui Second People's Hospital from January 2022 to June 2024 were selected as the BC group,and 100 healthy women without blood relationship at the same time were selected as the control group.The polymorphisms of CaSR gene rs17251221,rs60388563 loci were detected by polymerase chain reaction(PCR).The clinical data,genotype distribution and allele frequency were compared between the two groups.Non-conditional Logistic regression model was used to analyze the correlation between CaSR gene polymorphism and genetic susceptibility of BC patients.Results Comparing the general data of the two group,the proportion of family history of cancer in the BC group was significantly higher than that in the control group,and the difference was statistically significant(t=12.246,P<0.05).The genotype distribution of CaSR gene rs17251221 and rs60388563 loci in the control group and the BC group was consistent with the Hardy-Weinberg equilibrium law(χ2=0.087～1.202,P＞0.05),which was representative of the population.Under the co-dominant model of rs17251221 and rs60388563 loci,the risk of BC in GG genotype was significantly increased(OR=1.493,95%CI=1.070～2.108;OR=1.483,95%CI=1.034～2.121).Under the dominant model(AA vs GA+GG)and recessive model(GG vs GA+AA),the rs17251221 locus carrying G allele(OR=1.371,95%CI=1.023～1.824)and A allele(OR=0.524,95%CI=0.221～0.926)was significantly associated with the risk of BC(P<0.05).Under the dominant model(CC vs GC+GG)and recessive model(GG vs GC+CC),the rs60388563 locus carrying G allele(OR=1.245,95%CI=1.107～1.461)and C allele(OR=0.682,95%CI=0.523～0.974)was significantly associated with the risk of BC(P<0.05).Conclusion The rs17251221,rs60388563 loci polymorphisms of CaSR gene are closely related to BC susceptibility,and the risk of BC is high in individuals carrying G allele.

Objective To investigate the correlation between the expression of lectin galactoside binding soluble-3 binding protein(LGALS3BP),GTP enzyme activating protein SH3 functional region binding protein 1(G3BP1)and Wnt/β-catenin pathway genes in endometrial cancer(EC)tissues and its clinical prognostic significance.Methods 138 patients with EC treated in Cangzhou Central Hospital from February 2016 to February 2019 were selected.qRT-PCR was used to detect the expression of LGALS3BP mRNA,G3BP1 mRNA and Wnt/β-catenin pathway genes Wnt5a mRNA,β-catenin mRNA and matrix metalloproteinase-9(MMP-9)mRNA in cancer and adjacent tissues.The expression of LGALS3BP protein and G3BP1 protein in cancer and adjacent tissues was detected by immunohistochemistry.Pearson correlation analysis was used to analyze the correlation between LGALS3BP mRNA,G3BP1 mRNA and Wnt/β-catenin pathway genes.Kaplan-Meier curve was used to analyze the effect of LGALS3BP mRNA and G3BP1 mRNA on the survival and prognosis of EC patients.COX regression model was used to analyze the factors affecting the prognosis of EC patients.Results The expression of LGALS3BP mRNA(3.01±0.34),G3BP1 mRNA(2.87±0.33),Wnt5a mRNA(2.29±0.26),β-catenin mRNA(3.25±0.41)and MMP-9 mRNA(2.68±0.36)in EC cancer tissues were higher than those in adjacent tissues(1.10±0.23,1.06±0.24,0.84±0.17,0.88±0.26,0.69±0.17),and the differences were statistically significant(t=52.109～58.719,all P<0.001).The expression of LGALS3BP mRNA and G3BP1 mRNA in EC was positively correlated with the expression of Wnt5a mRNA,β-catenin mRNA and MMP-9 mRNA(r=0.675～0.781,all P<0.001).The expression of LGALS3BP protein(3.54±0.47 vs 0.51±0.16)and G3BP1 protein(2.84±0.44 vs 0.42±0.13)in EC cancer tissues were higher than that in adjacent tissues,and the differences were statistically significant(t=71.692,61.962,all P<0.001).The expression of LGALS3BP mRNA and G3BP1 mRNA in cancer tissues of EC patients with FIGO stage Ⅲ and lymph node metastasis were higher than that of FIGO stage Ⅰ～Ⅱ and no lymph node metastasis,and the differences were statistically significant(t=40.279～557.671,all P<0.001).The 5-year survival rate of LGALS3BP mRNA high expression group was 58.82%(40/68),which was lower than that of low expression group 94.29%(66/70),and the difference was statistically significant(Log-rank χ2=24.970,P<0.001).The 5-year survival rate of G3BP1 mRNA high expression group was 62.12%(41/66),which was lower than that of low expression group 90.28%(65/72),and the difference was statistically significant(Log-rank χ2=15.960,P<0.001).FIGO stage Ⅲ,lymph node metastasis,high expression of LGALS3BP mRNA and high expression of G3BP1 mRNA were risk factors for poor prognosis of EC patients(Wald χ2=7.847～12.054,all P<0.001).Conclusion The expression of LGALS3BP and G3BP1 mRNA is elevated in EC,both of which are associated with Wnt/β-catenin pathway genes,promoting the malignant progression of EC tumors,and are new tumor markers for evaluating the prognosis of EC patients.

Objective To investigate the expression of NOP2/Sun RNA methyltransferase family member 2(NSUN2)and protein arginine methyltransferase 5(PRMT5)in retinoblastoma(Rb)tissue and their correlation with the survival prognosis of Rb patients.Methods From February 2019 to February 2021,84 patients with Rb(Rb group)were selected from Beijing Anzhen Hospital Affiliated to Capital Medical University Nanchong Hospital,and 50 normal retinal tissues were used as control group.Real-time fluorescence quantitative PCR(qRT-PCR)was used to detect the levels of NSUN2 mRNA and PRMT5 mRNA in retinal tissues.Kaplan-Meier curve was used to analyze the effect of NSUN2 mRNA and PRMT5 mRNA on the prognosis of Rb patients.COX regression analysis was used to analyze the factors affecting the prognosis of Rb.Results The expression of NSUN2 mRNA(3.11±0.42)and PRMT5 mRNA(2.84±0.39)in Rb cancer tissues were higher than that in the control group(0.80±0.23,0.76±0.20),and the differences were statistically significant(t=35.935,35.033,all P<0.001).The expression of NSUN2 mRNA and PRMT5 mRNA in Rb cancer tissues with tumor diameter≥20mm,undifferentiated and ⅡRC stage D～E were higher than that in tumor diameter<20mm,differentiated tissues and ⅡRC stage A～C cancer tissues,and the differences were statistically significant(t=18.297,141.770,16.693;18.663,139.144,39.947,均P<0.001).The 3-year progression-free survival rate in the high expression group of NSUN2 mRNA was lower than that in the low expression group(60.00%vs 88.64%).The 3-year progression-free survival rate in the high expression group of PRMT5 mRNA was lower than that in the low expression group(56.10%vs 93.02%)(Log rank χ2=13.440,19.501,all P<0.001).Undifferentiated type,ⅡRC stage D-E stage,NSUN2 mRNA high,PRMT5 mRNA high were risk factors affecting the prognosis of Rb patients(Waldχ2=5.923～7.161,all P<0.001).Conclusion The expression of NSUN2 mRNA and PRMT5 mRNA in Rb tissues is increased,which is related to the maximum diameter of tumor,pathological classification and ⅡRC stage,and can be used as a tumor marker to evaluate the survival prognosis of Rb.

Objective To investigate the effect and mechanism of zanthoxylum bungeanum seed oil on autophagy and apoptosis of laryngeal cancer cells.Methods Human bronchial epithelial cells BEAS-2B and human laryngeal cancer cells Hep-2 were treated with different volume fractions(v/v)of Zanthoxylum bungeanum seed oil(0,0.02%,0.04%,0.06%,0.08%,0.10%),respectively,cell viability was detected by cell counting kit-8 method(CCK-8).Hep-2 cells in logarithmic growth phase were randomly divided into control group,Zanthoxylum bungeanum seed oil low,medium and high dose groups and Zanthoxylum bungeanum seed oil high dose+insulin-like growth factor 1(IGF-1)(PI3K agonist)group,cell apoptosis and cell cycle progression were detected by flow cytometry(FCM)assay,the formation of autophagic vesicles was detected by monodansyl cadaverine(MDC)staining,the expression of apoptosis,autophagy and phosphatidylinositide 3-kinases(PI3K)/protein kinase B(AKT)/mechanistic target of rapamycin(mTOR)pathway-related proteins were detected by Western blotting(WB).Results Compared with the control group Hep-2(99.03%±0.82%),treatment with zanthoxylum bungeanum seed oil(0.02%,0.04%,0.06%,0.08%,0.10%)(v/v)could reduce cell survival rate(84.63%±0.73%,57.34%±0.84%,19.76%±0.62%,17.22%±0.72%,12.19%±0.81%),and the differences were statistically significant(t=22.718～133.559,all P<0.001),while it has no inhibitory effect on BEAS-2B activity(t=0.283～1.980,all P>0.05).Compared with the control group,the Hep-2 apoptosis rate,G1/G0 phase cell proportion,autophagic vesicle integrated optical density(IOD)value,Cleaved-caspase-3,Bcl-2 associated X protein(Bax),microtubule-associated protein 1A/1B 1ight chain 3I/Ⅱ(LC 3I/Ⅱ)and Beclin-1 expression were all increased in the low,medium,and high-close groups of zantheoxylum bungeanum seed oil(t=4.270～58.425);the proportion of G2/M phase cells,ubiquitin-binding protein P62,Bcl-2 expression and p-PI3K/PI3K,p-AKT/AKT and p-mTOR/mTOR expression were all decreased(t=3.041～58.765),and the differences were statistically significant(all P<0.05).Compared with the high-dose zanthoxylum bungeanum seed oil group,the apoptosis rate of Hep-2,the proportion of G1/G0 phase cells,the expression of Cleaved-caspase-3,Bax,LC3Ⅱ/I and Beclin-1,the IOD value of autophagic vesicles(t=4.931～39.507),the expression of Bcl-2 and ubiquitin-binding protein P62,the proportion of G2/M phase cells,the ratio of p-PI3K/PI3K,p-AKT/AKT and p-mTOR/mTOR in the high-dose zanthoxylum bungeanum seed oil+IGF-1 group were decreased(t=3.402～14.207),and the differences were statistically significant(all P<0.05).Conclusion Zanthoxylum bungeanum seed oil can promote autophagy and apoptosis of Hep-2 cells,and its mechanism may be related to the inhibition of PI3K/AKT/mTOR pathway activation.

Objective To analyze the changes in serum levels of Krüppel-like transcription factors(KLF11)and long non-coding RNA small nucleolar RNA host gene 12(lncRNA SNHG12)in patients with intracranial aneurysm(IA),and their predictive value for prognosis.Methods A retrospective analysis was performed on 132 IA patients(IA group)who underwent interventional embolization for IA rupture and bleeding from February 2019 to February 2023,and 60 healthy people who underwent outpatient physical examination during the same period were selected as the control group.Serum levels of KLF11 was detected by enzyme-linked immunosorbent assay(ELISA),the level of lncRNA SNHG12 was detected by real-time fluorescence quantitative PCR(qRT-PCR).According to the modified Rankin scale(MRS)score,IA patients were divided into a good prognosis group(n=98,MRS score 0～2)and poor prognosis group(n=34,MRS score 3～6).Pearson correlation analysis was used to analyze the correlation between serum KLF11,lncRNA SNHG12 and cerebral hemodynamic parameters.Logistic regression analysis was used to analyze the factors affecting the prognosis of IA patients.The receiver operating characteristic curve was used to analyze the prognostic value of serum KLF11 and lncRNA SNHG12 in IA patients.Results The serum KLF11(47.12±6.58ng/L)and lncRNA SNHG12(1.89±0.36)in the IA group were lower than those in the control group(113.89±19.35ng/L,3.24±0.58),and the differences were statistically significant(t=19.695,35.476,all P<0.05).The levels of serum KLF11 and lncRNA SNHG12 in IA group were positively correlated with cerebral blood flow,cerebral blood volume and mean transit time(rKLF11=0.722,0.627,0.752;rlncRNA SNHG12=0.630,0.714,0.766,all P<0.05),and negatively correlated with intracranial pressure(r=-0.658,-0.599,all P<0.05).The proportion of CT Fisher grade 3～4 in IA patients in the poor prognosis group was higher than that in the good prognosis group,and the postoperative complication rate was higher than that in the good prognosis group,the serum KLF11(35.98±6.11 ng/L)and lncRNA SNHG12(1.12±0.30)levels were lower than those in the good prognosis group(50.98±6.90ng/L,2.16±0.39),and the differences were statistically significant(t=4.630～14.151,all P<0.05).CT Fisher grade 3～4,postoperative complications were risk factors for poor prognosis of IA patients(Wald χ2=8.403,12.049,all P<0.001),serum KLF11,lncRNA SNHG12 were protective factors(Wald χ2=5.550,7.904,all P<0.001).The AUC(95%CI)for predicting the prognosis of IA patients with the combination of serum KLF11 and lncRNA SNHG12 was 0.921(0.889～0.942),which was higher than the single detection of serum KLF11 and lncRNA SNHG12 at 0.848(0.805～0.886)and 0.810(0.767～0.852),and the differences were statistically significant(Z=5.886,4.367,all P<0.001).Conclusion The levels of serum KLF11 and lncRNA SNHG12 in IA patients are decreased,which are related to cerebral hemodynamic parameters.The combined detection has a high evaluation value for the prognosis of IA patients.

Objective To investigate the relationship between serum levels of micro RNA(miR)-497-5p,fibroblast growth factor-2(FGF2)and brain-derived neurotrophic factor(BDNF),with cognitive dysfunction in Parkinson's disease(PD)patients.Methods From April 2022 to April 2024,86 PD patients(study group)treated in Wuhan Xinzhou District People's Hospital and 60 healthy individuals(control group)who underwent physical examination in Wuhan Xinzhou District People's Hospital were selected.Serum miR-497-5p levels were determined by real-time quantitative polymerase chain reaction(RT-qPCR),and serum FGF2 and BDNF levels were measured by enzyme linked immunosorbent assay(ELISA).Spearman was applied to analyze the correlation between serum miR-497-5p,FGF2,BDNF levels and montreal cognitive assessment(MoCA score).Logistic analysis was applied to analyze the factors influencing cognitive dysfunction in PD patients.Receiver operating characteristic(ROC)was applied to analyze the diagnostic value of serum miR-497-5p,FGF2 and BDNF for cognitive dysfunction in PD patients.Results The serum miR-497-5p(2.73±0.67)expression level in the study group was obviously increased than that in the control group(1.04±0.34),while the expression levels of FGF2(1.94±0.45ng/ml)and BDNF(8.31±2.44ng/ml)were obviously reduced than those in the control group(2.71±0.69ng/ml,12.81±3.07ng/ml),with significate differences(t=17.977,8.161,9.850,all P<0.05).Serum miR-497-5p was negatively correlated with MoCA score(r=-0.331,P<0.05),while FGF2 and BDNF levels were positively correlated with MoCA score(r=0.404,0.361,all P<0.05).Cognitive dysfunction group had significantly higher disease duration,FGF2,BDNF levels,and MoCA scores than the cognitively normal group.The course of disease and miR-497-5p levels in the cognitive dysfunction group were obviously higher than that in the normal cognitive function group,FGF2,BDNF levels,and MoCA scores in the cognitive dysfunction group were obviously lower than that in the normal cognitive function group(t=2.350～11.792,all P<0.05).The course of disease and serum miR-497-5p were risk factors for cognitive dysfunction in PD patients(Wald χ2=4.712,5.704,all P<0.05),while MoCA score,serum FGF2,and BDNF were protective factors for cognitive dysfunction in PD patients(Wald χ2=4.499,5.556,5.217,all P<0.05).The AUC and sensitivity of the combination of serum miR-497-5p,FGF2,and BDNF in PD patients with cognitive impairment were higher than those of individual diagnosis.The diagnostic effect of the combination of miR-497-5p,FGF2,and BDNF in PD patients with cognitive dysfunction was better than that of individual diagnosis,and the differences were statistically significant(Z=2.279,2.236,2.123,all P<0.05).Conclusion Elevated serum miR-497-5p level and decreased FGF2 and BDNF levels can increase the risk of cognitive dysfunction in PD patients,and the combination of the three has good diagnostic value for cognitive dysfunction in PD patients.