Objective To understand the different types of chronic hepatitis group in shenzhen area E viral hepatitis (hepatitis E infection status and provide scientific basis for the prevention and treatment of hepatitis E.Methods Randomly collected from July 2013 to June 2015 in shenahen Longhua New District People’s Hospital without hepatitis normal physical exami-nation and treatment group 1746 cases as control group,chronic hepatitis B viral hepatitis (hepatitis B)the crowd of 1 320 cases of hepatitis B,chronic viral hepatitis C (HCV)population group,615 cases of hepatitis C,respectively,the application method of enzyme-linked immunosorbent assay (ELISA)to detect serum hepatitis E antibody (anti HEV IgG)-IgG,ana-lyzed of different types of hepatitis a crowd hepatitise infection status,and compared the hepatitise infection rate if there was a difference between the different groups.Results 1 746 cases of control group in serum anti HEV-IgG positive rate was 3.49% (61/1 746),4.22% of men and women in 2.68%.1 320 cases of hepatitis B group was 10.9%,12.29% of men and women in 8.23%.615 cases of C group was 10.2%,12.35% of men and women in 7.64%.Hepatitis B and C group of HEV-IgG positive rate compared with control group difference was statistically significant (χ2 =9.163~9.405,P <0.05), and hepatitis B and hepatitis C group there was no statistically significant difference between positive HEV-resistant IgG (χ2=0.614,P >0.614),and hepatitis E infection rate than women,men was statistically significant difference (χ2 =2.873~4.025,P <0.05).Conclusion Chronic hepatitis B and C viral hepatitis crowd anti HEV-IgG positive rate was higher than normal people,no hepatitis hepatitis E infection rate among men than women.Therefore,strengthens to the chronic hepatitis B and C viral hepatitis a crowd of early discovery,early diagnosis and early treatment,to reduce hepatitis E infection rate has an important significance.

Objective To investigate serum alpha 1-acid glycoprotein (AAG)in patients with rheumatoid arthritis (RA)pa-tients with serum drug before and after the treatment,application of evaluation of serum alpha 1-acid glycoproteindetection in RA.Methods 88 cases of newly diagnosed RA patients treated with methotrexate,hydroxychloroquine combined with pyri-dine and the Liu Danhuang administration,in zeroth and June.The patients were taken for determination of serum AAG and rheumatoid factor in the serum of the patients with rate nephelometry (RF)levels,and erythrocyte sedimentation rate (ESR),and record the patients with RA disease the activity index of 28 (DAS28).Results After treatment,RA in the blood of patients with AAG (1 136.30±322.40 mg/L),RF(43.73±4.53 IU/ml),ESR (34.56±9.21 mm/h)and DAS28 (3.10 ±1.0)were significantly decreased (P <0.05 or 0.01),and the AAG,ESR,RF and DAS28 were positively correlated (r=0.19,P =0.02;r=0.24,P =0.0 and r=0.39,P =0.03).Conclusion The serum RA inpatients with AAG changes obvi-ously,had certain curative effect evaluation of RA.

Objective To analyze the epidemiological characteristics of Epstein Barr virus (EBV)or cytomegalovirus (CMV) infection in systemic lupus erythematosus (SLE)patients in order to provide reference for clinic.Methods The clinical data of 202 female cases in-patients diagnosed with SLE (SLE group)from January 2012 to May 2015 in Nanjing Drum Tower Hospital were analyzed retrospectively.Meanwhile,203 cases including female renal transplant donors,obstetrics and gyne-cology in-patients selected randomly were enrolled as control group.The infection rate between SLE and control groups was analyzed and compared based on the results of EBV-DNA and CMV-DNA in peripheral blood quantified by real time PCR. Results The positive rate of EBV-DNA in SLE group was 11.39% (23/202),with significantly statistical difference when comparing with the control group [3.45% (6/174)](χ2 = 8.28,P < 0.01).The positive rate of CMV-DNA [7.92% (16/202)]was significantly higher than that in control group [1.97% (4/203)](χ2 =7.64,P <0.05).In addition,the positive rate of EBV-DNA and CMV-DNA was also greatly higher in reproductive-age (20~45 years old)of SLE patients than those in control group,10.94%(14/128)vs 3.45%(4/116)(χ2 =4.99,P <0.05)and 7.75%(10/129)vs 2.22%(3/135)(χ2 =4.31,P <0.05),respectively.Conclusion SLE patients were more inclined to be accompanying infected by EBV or CMV, indicating the possible correlation between SLE and EBV or CMV infection;and physicians should pay more attention to the viral infection of SLE in clinical treatment.

Objective To explore the relationship between Lp (a)level and age,gender in Nanjing area.Methods 8 442 ser-um specimens from examination individuals were collected in the First Affiliated Hospital of Nanjing Medical University in 2014.Lp (a)was measured by immune turbidimetry method.All the subjects were divided into six groups:15~29,30~44, 45~59,60~69,70~79 and ≥80 years old.SPSS21.0 software was used to carry out statistics.Results The Lp(a)level in female [134±197 mg/L (M±QR,the same below)]was higher than that in male significantly (U =8 355 137,P <0.001). LP (a)levels from people of different sex correlated with the age weakly (r =0.154,P <0.001),and increased with age groups.Lp(a)of six groups in male were 106.5± 151.0,119.0± 170.0,128.0± 179.0,159.0±206.0,145.0±200.0 and 162.0± 190.0 mg/L respectively.The difference was statistically significant between between 1 and 2 groups,2 and 3 groups,3 and 4 groups,theU value were 645 152.5,1 006 572.0,197 595.0 respectively;P all<0.05.The difference was not statistically significant between 4 and 5 groups,5 and 6 groups,3 and 4 groups,theU value were 59 127.0 and 15 959.5 respectively,P >0.05.Lp(a)of six groups in female were 128.0 ± 194.0,128.0 ± 187.0,139.0 ± 207.0,157.0 ± 228.0, 173.5±227.0 and 150.0±201.0 mg/L.The difference was statistically significant between 2 and 3 groups,theU value was 641 147,P =0.006,respectively.The difference was not statistically significant between 1 and 2 groups,3 and 4 groups,4 and 5 groups,5 and 6 groups,theU value were 783 676,92 442.5,16 069.5 and 3 038,respectively;P all>0.05.Conclusion Lp (a)level in healthy population in Nanjing area is related to gender and age groups,it is necessary to establish different reference ranges.

Objective To develop a HPLC-MS/MS method for determination of Imatinib and Dasatinib in CML patient,and make it used in clinic trial.Methods The separation was performed on a Ultimate XB-C18 column with a mobile phase of water(containing 2 mmol/L ammonium acetate and 0.1 ml/dl formic acid)and methanol(containing 0.1 ml/dl formic acid). The way of eluting was gradient.Mass spectrum detection method was ESI positive ion mode and monitoring Imatinib m/z 494.5>394.3 and Dasatinib m/z 488.3>401.3.Results The standard curve of Imatinib was linear over the range of 0.05~7.5 μg/ml,Y =5.6×105 X+5× 103 (R =0.999 8).Thestandard curve of Dasatinib was linear over the range of 5~250 ng/ml,Y =211X+66.6(R=0.999 6).The relative recovery was among the range of 90%~107%.RSDs of intra-and inter-day validation were less than 10%.Conlusion This method is convenient,accurate and rapid,and can be used for the deter-mination of Imatinib and Dasatinib in clinic test.

Objective To validate the analytical performance of four LP(a)reagents with Immunoturbidimetry method used on the automatic biochemistry analyzer for preliminary clinical application.Methods The performance of four LP(a)reagents (labeled as A,B,C,D)with method from RANDOX,Zhejiang Kuake Co.,Beijing Leadman Co.and Beijing Jiuqiang Co.on Olympus AU5800 automatic biochemistry analyzer were assessed.The precision,linearity range,accuracy,disturbance (vita-min C,bilirubin,hemoglobin,TG)were assessed.Results The within-run CVs of the four reagents (A,B,C and D)were 0.64%~1.18%,3.59%~4.75%,1.33%~3.05% and 1.43%~2.01% respectively.The between-run CVs in A,B,C and D were 1.04%~1.7%,3.81%~4.93%,2.16%~4.76% and 2.33%~3.21% respectively,lower than the stated.The lin-earity range was 82~923 mg/L (r2 =0.997),130~935 mg/L (r 2 =0.996 4),120~1025 mg/L (r 2 =0.992 1)and 117~943 mg/L (r2 =0.999 5)in the four reagents,which demonstrated a sound linear correlation.For interference tests,no re-markable interferences (<±10%)of reagent A and reagent D were detected when Vitamin C≤10 mg/dl,hemoglobin≤200 mg/dl,bilirubin≤40 mg/dl and TG≤500 mg/dl.Interference of reagent B was found when VC≥5 mg/dl,TG≥250 mg/dl and when TG≥250 mg/dl reagent C was interfered significantly.The four LP(a)reagents were used to detect the lipid con-trol,and the deviations of the target value were - 8.07%,1.34%,- 8.05% and 7.38% respectively.Conclusion When used in automatic biochemical analyzer,the four LP(a)reagents showed high precision.The four reagents are all able to meet clinical test requirements,nevertheless,anti-interference capability were different.

Objective To estimate the assay performance of two different kind of reagent with different concentration of NADH.Methods Verificated function index of these two reagents,such as accuracy,sensitiveness,blank space and so on, and determined the molar extinction coefficient of NADH using hexokinase method and calculated the NADH density in two kind of AST reagents.Then estimated the effect of concentation of NADH on the analysis performance.Results NADH concentration was 0.21 mmol/L,and the sample detection limit of the linear was 1 629 U/L.NADH concentration was 0.13 mmol/L,and the sample detection limit of the linear was 1 263 U/L.So the gap between the two reagent’s detection linear was visible.But there were no significant changes in other performance indicators such as detection sensitivity,reagent blank,precision.Conclusion The NADH concentration in the reagent of AST had greatimpact on the detecting linear range, so should pay attention to the potential change of linear range in the daily work.

Objective To investigate the causes on platelet distribution width (PDW)results not shown in the automatic he-matology analyzer and evaluate the accuracy of the platelet results of these samples with the automatic hematology analyzer. Methods The platelet morphology was observed in microscope for the specimen which PDW were not shown in the auto-matic hematology analyzer.And the platelet results counted in microscope were statistically compared with that in the auto-matic hematology analyzer.Results In the 200 specimens which PDW were not shown in automatic hematology analyzer, there were 104 specimens(52%)in which large platelet was found,36 cases(18%)in which platelet aggregation was visible, 28 cases(14%)in which the microcytes or erythrocyte debris could be seen,32 cases(16%)in which the obvious abnormal was not found.The platelet results counted in microscope for the specimens,in which large platelets,platelet aggregation or microcytes were found,were very different with the results counted with the automatic hematology analyzer(P < 0.05).The PDW of the 200 specimens were rechecked in the automatic hematology analyzer.And 64 cases (32%)PDW results were got,of which 55 cases(85.9%)PDW results were beyond the normal range.Conclusion The main causes for the PDW not shown in automatic hematology analyzer includes large platelets,platelets aggregation and microcytes etc.The platelet re-sults in these specimens by automatic hematology analyzer were different with that counted in microscope.Therefore,the platelet of these specimens should be counted in microscope.

Objective To evaluate diagnostic values of thirteen single and combined serum tumor markers in the diagnosis of lung cancer in Chinese people.Methods Chinese databases were searched systematically for prospective studies of serum tumor markers in Chinese patients with lung cancer and standard statistical methods for meta-analysis were applied.Results Thirty articles were selected containing thirteen types of molecular tumor markers and 4 393 people.The optimal serum marker was CEA+CA125+CYFRA21-1 with the combined sensitivity,specificity,positive likelihood ratio,negative likeli-hood ratio,the diagnostic odds ratio and the area under the summary receiver operating characteristic curve (AUC)was 87%[95% confidence interval (CI)0.81~0.91],93%(95% CI 0.89~0.95),11.8 (95% CI 7.84~17.7),0.15 (95% CI 0.10~0.21),81.3(95% CI 44.4~149.0)and 0.910,respectively.Conclusion There was improved diagnostic performance in combined markers than other individuals.Serum tumor marker CEA+CA125+CYFRA21-1 is the optimal biomarker for the diagnosis of lung cancer.

Objective To evaluate the impact of human papillomavirus(HPV)infection on the risk of HIV acquisition in the female group.Methods Searched PubMed,EMbase,Coehrane Library,CNKI,wanfang database and Chinese Biomedical Lit-erature Database etc for articles about HIV-acquisition in HPV infected female patients.The quality of the literature were e-valuated according to standards of inclusion and exclusion.Data was extracted and methodologically quality evaluated by two independent investigators.Meta-analysis was accomplished using RevMan4.2 software.Results 6 articles were included,all of them were randomized controlled trials,a total of 9 606 cases studied.HIV infection risk was doubled in women with any HPV infection(OR= 2.02,95% CI:1.48 ~ 2.77),demonstrating High-risk HPV-positive (OR= 2.50,95% CI:1.73 ~3.61)and low-risk HPV-positive (OR=2.10,95% CI:1.48~2.96)respectively.Funnel plot analysis revealed no signifi-cant publication bias on HPV genotypes.Conclusion The analysis of selected research suggests the risk of HIV infection was increased in HPV-positive women.HPV vaccine may have some preventive effect on HIV infection.