Objective: To assess the regional systolic and diastolic function of left ventricle before and after coronary stent implantation by pulsed wave Doppler tissue imaging (PW-DTI). Methods: Twenty-six patients with coronary artery disease ( CAD) involving only left anterior descending coronary artery ( LAD) were examined within 1-3 days before, 7 days and 30 days after coronary stenting with PW-DTI. Peak systolic , peak early diastolic, and peak late diastolic motion velocities ( Sm, Em, and Am respectively) were measured at 6 different sites on the basal and medial segments corresponding to the anteroseptal, postero-septal, lateral, anterior, inferior, and posterior walls of the left ventricle. Results:In corresponding segments depending on LAD blood flow supply, Sm was increased significantly 7 days after the stenting proce- dure. Em was also increased 7 days after the procedure in most of the corresponding segments. There were little changes in other segments that were independent on LAD blood flow supply. Conclusion: These findings suggest that there is an early improvement in the regional systolic and diastolic function of the left ventricle after the coronary stenting, and that PW-DTI techniques could evaluate quantitatively the regional ventricular performance in patients receiving percutaneous coronary intervention.

Objective:To discuss the protective effect of intestinal ischemic preconditioning(iIPC) on the myocardium and the role of uncoupling protein 2(UCP2) in myocardium with iIPC. Methods: The animal model was built by superior mesenteric artery ligation of rats.Then they were subjected to a sustained coronary occlusion of 30 min and reperfusion of 180 min.We evaluated the degree of the myocardial damage and used RT-PCR method to check the relative content of UCP2 mRNA.The IR control group underwent the coronary occlusion/ reperfusion with no intestinal ischemia and the blank group was sham-operated. Results:①Compared to the blank group,the IR control group showed much heavier damage in the ultramicro structure.And for the iIPC group, the damage in the ultramicro structure 0 h and 24 h after iIPC was lighter than that of the IR control group.②Compared to the expression of the UCP2 mRNA in the myocardium of rats in the IR control group,the expressions of iIPC 0 h,6 h,12 h,24 h,48 h groups increased substantially(P

Computed tomographic(CT) pulmonary angiography is becoming the standard of care for the evaluation of patients with suspected pulmonary embolism.This pathologic condition,whether acute or chronic,causes both partial and complete intraluminal filling defects.In acute pulmonary embolism that manifests as complet arterial occlusion,the affected artery may be enlarged.Partial filling defects due to acute pulmonary embolism are often centrally located,but when eccentrically located they form acute angles with the vessel wall.Chronic pulmonary embolism can manifest as complete occlusive disease in vessels that are smaller than adjacent patent vessels.In this article,the author described the technique of CT pulmonary angiography,diagnostic criteria for acute and chronic pulmonary embolism,and causes of misdiagnosis of pulmonary embolism.

Studies on ORC in the cell cycle were limited,this paper is to explore origin recognition complex(ORC)in the cell cycle,and elucidate the relation between them.

Objective:To explore the result of application of the clinical nursing pathway on percutaneous coronary interventional patient.Methods:One hundred patients were divided into two groups randomly,each group has 50 cases.We used the clinical nursing pathway based on holistic nursing case in experimental group,and the control group used the holistic nursing care.Results:After use the method of the clinical nursing pathway in the hospital,the medical cost and the rates of the nursing errors were less than that in the comparing group while the patients were more satisfied.Conclusion:As a scientific mode,the clinical nursing pathway could improve the working efficiency,guarantee the nursing quality.

Wnt signaling pathway has a critical role in differentiation, proliferation, supersession and metabolism of osteocyte. Although the principal components are relatively simple, however, the numerous components consisted of Wnt signaling participate in the reaction, including several LRPs ( low density lipoprotein receptor-related protein,LRP) , 19 different Wnt proteins and 19 different FZDs( frizzled receptor, FZD) , and non-canonical Wnt signaling and multiple inhibitors of Wnt signaling.The Wnts linked to mem-brane coreceptor and activated downstream of canonical Wnt signaling or non-canonical Wnt signaling, In addition, different Wnts had different roles in differentiation, bone formation and bone absorption of osteocyte.This review aims to understand the relationship be-tween Wnt signaling and osteocyte metabolism and bone diseases, by analyzing a variety of factors and the process of Wnt protein and Wnt signaling pathway in activity of osteocyte.

Assessing the stage of live fibrosis and cirrhosis has direct clinical implications for patient treatment options.Liver biopsy is typically considered as the gold standard, but it has limited clinical utility due to its invasiveness.In recent years, with the rapid development of CT hardware and software, CT has made important progress in the non-invasive evaluation of liver fibrosis and cir-rhosis at home and abroad.This review focuses on clinical application of of contrast-enhanced CT, CT perfusion imaging, dual-energy CT and CT molecular imaging for liver fibrosis and cirrhosis.

Radiation proctitis is one of the complications after radiotherapy for pelvic malignancies.Surgery is not necessary for the most of radiation proctitis.However, surgery is unavoidable when rectal obstruction, uncontrollable bleeding, and fistula occur. This article reviews the progress in surgical treatment of radiation proctitis.

Obj cetive A large number of recent studies show that sevoflurane anesthesia may cause learning and memory dysfunction.The aim of this study was to explore changes of learning and memory ability and hippocampal volume in infantile rats after neonatal interrupted and repeated inhalation of 2.6% sevoflurane through detecting the learning and memory ability by Morris water maze and the hippocampus volume by MRI.Method s Thirty two neonatal SD rats were randomly devided into two groups (n=16):experimental group and control group.Rats inhalated 2.6%sevoflurane in the experimental group and 1 L/min O2 +1 L/min Air in the control group at the postnatal days of 7, 14 and 21 (P7, P14, P21). The learning and memory ability was determined by the Morris water maze test from P31 to P37;The brains of rats were scanned by mag-netic resonance imaging ( MRI) machine under anesthesia with 1%sodium pentobarbital at P37, and the brain and bilateral hippocampal volumes were measured. Results ①In the place navigation test, the escape latency had no significant difference between the two groups (P>0.05).In the spatial probe test, the dwelling time, movement distance and number of entering times in platform quadrant decreased slightly in experimental group compared with those in the control group, while there was no significant difference (P>0.05).②The brain volume [(1.53 ±0.18) cm3 vs (1.60 ±0.13) cm3] and right hippocampal volume [(16.15 ±1.76)mm3 vs(16.46 ±1.71)mm3] had no significant difference between the two groups (P>0.05).The left hippocampal volume [(16.46 ±1.71)mm3] was decreased in the experimental group compared with the control group [(18.10 ±2.53)mm3](P<0.05). Conclusion The learning and memory ability has no significant changes in in-fantile rats after neonatal interrupted and repeated sevoflurane inhalation and MRI examination of hippocampal volume is not sufficient for the diagnosis of cognitive dysfunction.

Objective PU.1 plays a key role in innate immune function in the alveolar macrophage.This study was to con-struct and identify recombinant adenovirus vectors carrying the human transcription factor PU.1 gene. Methods The recombinant shut-tle plasmid was obtained from the PU.1 gene ( SPI1) and eukaryotic expression vector that digested by restriction enzymes and connected by T4 DNA ligase.The target fragment SPI1-IRES-EGFP was amplified by PCR.The product was cloned into the intermediate pDONR221 and then recombined with the adenovirus backbone plasmid pAd/CMV/V5-DEST to form a recombinant adenovirus vector. The recombinant adenovirus vector was linearized by PacI and then transfected into human embryonic kidney (HEK293) cells to obtain the recombinant adenovirus pAD-SPI1-IRES-EGFP, which was then propagated in HEK293 cells, filtered and purified to obtain high-con-centration adenoviruses.The adenovirus titer was determined by TCID 50 assay.The PU.1 gene expression in the HEK293 cells was con-firmed by fluorescence microscopy and real-time qPCR. Results PCR amplification, restriction digestion and sequencing analysis showed the recombinant adenovirus carried the correct PU.1 gene.The final virus titer, calculated by TCID 50, was 8 ×1011 IU/mL. Green fluorescence was observed under the fluorescence microscope. Real-time qPCR confirmed that the expression of PU.1 mRNA was increased by 2189.93 folds. Conclusion The recombinant adenovirus vector carrying the PU.1 gene was constructed and obtained successfully, which could contribute to further studies of the influence of PU.1 overex-pression on the innate defense against Aspergillus fumigatus.