This case was diagnosed as multiple cementoosseous dysplasia on the basis of clinical & radiological features but was diagnosed as ossifying fibroma on the basis of histopathological feature. The histopathologic features of the multiple cementoosseous dysplasia and cementoossifying fibroma have common features of cementum, fibrous network and bone. Multiple cementoosseous dysplasia is reactive lesion and shows restricted lesion size, occurred on anterior and posterior tooth of the mandible and needs no treatment except periodic follow up. But Cementoossifying fibroma is the true neoplasm and grows continuously and needs surgical removal. The final diagnosis of the multiple cementoosseous dysplasia requires good correlation of the clinical histopathological, and radiological features.
Dental Cementum ; Diagnosis ; Fibroma ; Fibroma, Ossifying ; Follow-Up Studies ; Mandible ; Tooth

This report details a case of 8-year-old girl showing failure of odontogenesis after chemo-radiation therapy for rhabdomysarcoma at the age of 4. The observed results were as follows ; 1. Past history revealed that she had received for a total radiation dose od 4430cGy, 29 fractions in 6 weeks and chemotherapy with vincristine, actinomycin D and cytoxan, followed as maintenance phase for 2 years. 2. The patient was symptom-free and appointed for the treatment of multiple dental caries. 3. Oral examination showed hypoplastic enamel on whole erupted permanent teeth and showed retarded eruption. 4. Conventional radiograms showed failure of root development including abrupt cessation of root formation and root agenesis, and microdobtia, missing teeth, irregular enamel, dislocation of the impacted teeth. Additional finding showed good healing bone pattern on the left mandibular ramus and angle area. 5. Cehalometric analysis revealed failure of bite raising due to incomplete eruption of all the first molars and made it possible to suspect entrapped mandibular growth and then Class II tendency growth. 6. There was correlation between the time of chemo-radiation therapy and the damage of the teeth.
Child ; Cyclophosphamide ; Dactinomycin ; Dental Caries ; Dental Enamel ; Dental Enamel Hypoplasia ; Diagnosis, Oral ; Dislocations ; Drug Therapy ; Female ; Humans ; Molar ; Odontogenesis* ; Rhabdomyosarcoma* ; Tooth ; Tooth, Impacted ; Vincristine

The purpose of this study was to aid in the prediction of tumor cell tolerance to radiotherapy and/or chemotherapy. For this study, cell surviving curves were obtained for human squamous cell carcinoma KB cell line after radiation exposure and/or administration of antitumor drugs. 2, 4, 6, 8 10Gy were irradiated at a dose rate of 210Gy/min using 60Co Irradiator ALDORADO 8. After irradiation, KB cell lines(3*104cells/ml) were exposed to 2 /ml of bleomycin ir cisplatin for 1 hour. The viable cells were determined by MTT assay for each radiation dose and/or each drug at the 4th day. And they were compared to control values. The obtained results were as follows ; 1. The slope of the surviving curve after irradiation of 2, 4, 6, 8, 10Gy on KB cell line was relatively steep. 2. There was no significant difference between ths cytotoxicity of bleomycin compared to control group. But, there was significant difference between the cytotoxicity of cisplatin compared to control group. And the cytotoxicity of cisplatin was greater than that of bleomycin on KB cell line. 3. There were dignificant differences of surviving fractions after irradiation of 2Gy and 10Gy with 2 /ml of bleomycin compared with the groups of irradiation only on KB cell line. 4. There were significant differences of surviving fractions after irradiation of 2, 4, 6, 8, 10Gy with 2 /ml of cisplantin compared with the groups of irradiation only on KB cell line. 5, There was significant difference of surviving fraction between groups after irradiation of 10Gy with 2 /ml of bleomycin and cisplatin.
Antineoplastic Agents ; Bleomycin ; Carcinoma, Squamous Cell ; Cisplatin ; Drug Therapy ; Humans ; KB Cells* ; Radiation Tolerance* ; Radiotherapy

With the CT findings as gold standard, the sensitivity, the specificity, and the diagnostic accuracy of WatersO radiography and WatersO radiography with panoramic radiography were compared in the diognosis of antral mucosal thickening of 16 patients. Three oral radiologists and three non-oral radiologists interpreted the WatersO radiographs and after 4 weeks, interpreted the WatersO radiographs and panoramic radiographs simutaneously. The interpretation was the existence or the non-existence of the mucosal thickening on the medial, the posterolateral, the floor, and the roof of maxillary sinus. The obtained results were as followed ; 1. In oral radiologist group, the sensitivity, specificity and diagnostic accuracy of WatersO film were 0.7250, 0.8489 and 0.7578 respectively. 2. The sensitivity and the diagnostic accuracy in oral radiologist group were higher than those of non-oral radiologist group (P<0.05), but there was no significant difference between two groups in the specificity>0.05). 3. There was no significant difference of the diagnostic abilities except the specificity in oral radiologist group between WatersO radiography and WatersO radiography with panoramic radiography (p>0.05). 4. The sensitivity and the diagnostic accuracy were the highest in the case of medial wall interpretation, the specificity was the highest in the posterioateral wall. 5. In the posterolateral wall and the floor, the sensiticity and the diagnostic accuracy of oral radiologist group were higher than those of non-oral radiologist group (P<0.05).
Diagnosis* ; Humans ; Maxillary Sinus ; Radiography* ; Radiography, Panoramic* ; Sensitivity and Specificity

Cellular transforming genes have been identified in a number of different tumor cell lines and tumor types. A significant number of these oncogenes belong to the ras gene family. The ras gene family consists of three closely related genes:H-ras, K-ras and N-ras which code for a related 21 kDa protein. Mutations in codon 12, 13 and 61 of one of the three ras genes convert these genes into acute oncogenes. The presence of H-ras gene mutaions has important prognostic implication in various tumors. Each genomic DNA was isolated from tumors induced by implantation with DMBA, or by treatment with DMBA-implantation/irradiation. When genome DNA was transfected into NIH 3T3 cells and investigated by two-step PCR-RFLP, the following results were concluded : 1. Transformation foci developed in two groups when the genome DNA of two experimental groups were transfected into NIH 3T3 cells. 2. Transformation efficiency was 0.01-0.02 foci/ DNA in the experimental group with the DMBA-implantation, 0.01-0.03 foci/ DNA in the experimental group with the DMBA-implantation/irradiation according to results of trasfection assay. 3. When the point mutation of H-ras gene was investigated by a two-step PCR-RFLP, there was 13.9%(5/36) in the experimental group with the DMBA implantation, 15.4%(6/39) in the experimental group with the DMBA-implantation/irradiation. 4. The point mutation in codon 12 and 61 of H-ras was 5.6%(2/36) and 8.3%(3/36) in the experimental group with the DMBA implantation. 5. The point mutation in codon 12 and 61 of H-ras gene was 7.7%(3/39) in the experimental group with the DMBA-implantation/irradiation.
9,10-Dimethyl-1,2-benzanthracene* ; Animals ; Cell Line, Tumor ; Codon ; DNA ; Genes, ras* ; Genome ; Humans ; NIH 3T3 Cells ; Oncogenes ; Point Mutation ; Rats* ; Salivary Glands*

Cementifying fibroma is an odontogenic tumor of periodontal ligament origin, consistingof a proliferation of fibrous tissue that forms cementum. Cemento-ossifying fibroma, cemenrifying fibroma and ossifying firoma are difficult to distinguish on clinical and radiographic examination, and on histoapathology. However, when the calcified products is represented by ovoid or curvilinear deposites, the lesion is often referred to as cementifying fibroma. Cementifying fibroma of the jaws are well-circumscribed, generally slow-growing lesions. Although most lesions appear to be limited to the tooth-bearing areas, a few have extended into the angle-ramus area or encroached on the maxillary sinus. Radiographically, the cementifying fibroma could present as a radiolucent, radiopaque or mixed density lesion, depending on the degree of maturity. We have oberved two cases f cementifyingfibroma occurred in the left maxillary premolar-molar area of 38-year-old woman and 35-year old man. We obtained that two cases were shown the followed results ; 1. Clinically, main clinical sympton was facial swelling and pain at palpation. 2. Radiographically, well-circumscribed radiolucent lesion and radiolucent lesion with radiopaque foci were seen on the left maxillary premolar-molar areas. Loss of lamina dura and root resorption or divergency were also seen. 3. Histopathologically, many cementum-like calcifications were observed in the hypercellular connective tissue.
Adult ; Connective Tissue ; Dental Cementum ; Female ; Fibroma* ; Humans ; Jaw ; Maxillary Sinus ; Odontogenic Tumors ; Palpation ; Periodontal Ligament ; Root Resorption

We used five adult dog mandibles embedded in resin block and six different cross-sectional planes for each mandible were choosen. According to the angle of mandibular occlusal plane to vertical plane(mandibular angle) and gantry angle of CT machine, we classfied 4 experimental groups and 1 control group. The control group images were taken at the mandivylar angle 0 degrees (group 1) ;30 degrees and 0 degrees (group 2);15 degrees and 15 degrees (group 3);30 degrees and 30 degrees(group 4), respectively. Using the reformatted cross-sectional images, the distance from the mandibular canal to the alveolar crest and the distance from the mandibular canal to the buccal cortex and to the lingual cortex was measured and compared. The obtained results were as follows ; 1. The distance from the mandibular canal to the alveolar crest of group 1 and 2 was larger than control group, but the distance of group 3 and 4 was smaller. The distance from the mandibular canal to the buccal cortex and to the lingual cortex of all experimental groups was smaller than control group. 2. The distance from the mandibular canal to the alveolar crest showed the largest difference from control group in all experimental groups, especially in group 2 and 4(P<0.05). 3. In the distance from the mandibular canal to the alveolar crest, the number of deviation value under 1mm was 20 in group 3 and was 11 in group 2 and 4, respectively. 4. The deviation value of the distance from the mandibular canal to the buccal cortex and to the lingual cortex was under 1mm in most cases.
Adult ; Animals ; Dental Occlusion ; Dogs ; Humans ; Mandible*

Radiation-imparied wound healing in animal experiments was believed to be an another logical experimental model to understand the wound healing mechanism in patients. the purpose of this study was to reveal the block point which would result impaired healing. Twenty four rats(Sprague-Dawley stains) were divided into two gruops according to the time interval between irradiati on and wounding. Group I, observing the healing effect on the 1st day and group II are the healing effects on the 7th d ays after irradiation to the wound of the rat tongue. Experimental animals were sacrificed 3, 6, 12 and 24 hours after w ounding. The specimens were examined by the light microscope and trasmission electron microscope. The following results were obtained: 1. Fibroblasts in both goups showed degenerative changes which were dilated mitochondria and rER, reduced microorganell e, vacuoles and little cytoplasmic process. 2. Average length between bands and quantity of the newly produced collagen fibers aroud fibroblasts remained unchanged against control group. 3. The severity of degenerative change of the fibroblast and impairment of wond healing including shortening of the thi ckness of collagen fibers were more sever in the group II than in the group I.
Animal Experimentation ; Animals ; Collagen ; Cytoplasm ; Fibroblasts* ; Humans ; Logic ; Mitochondria ; Models, Theoretical ; Rats* ; Tongue* ; Vacuoles ; Wound Healing* ; Wounds and Injuries*

The purpose of this study was to investigate effects of osteoporosis on fracture wound healing in the calcium defi cient rat. To research the experiment some ten-week old Wistar strain rats with approximately 300 gms weight were selected. Then , the rats were divided into two groups: Normal diet group(rats given a normal diet before and after bone fracture) and Low calcium diet group(rats given a low calcium diet before and after bone fracture). Both groups had been provided wit h each diet for three weeks. When the rats became thirteen weeks old, the mandibular angle of rats in both groups was ar tificially fractured for test. The healing of fracture wounds was reviewed by using soft x-ray radiography and 99m Tc-MDP bone scan and also histopathologic examination. The obtained results were as follows : 1. The radiolucency of the fracture site for the Normal diet group started to decrease from the 14th day since the expe riment was made, while the Low calcium diet group began decrease in the radiolucency from the 21st day of the experiment . The radiolucency for the normal diet group disappeared at the 42nd day, but one for the Low calcium diet group disappe ared at the 56th day of the experiment. 2. The highest uptake rate of (99m)Tc-MDP stood at the 14th day of the experiment in the Normal diet group and the Low calium diet group's maximum rate was recorded at the 21st day of the experiment. These both groups were gradual ly experiencing decrease in the uptake rate as the experiment time was going on. However, the uptake rate in the Low cal ium diet group was lower than one in the Normal diet group. 3. For the Normal diet group, the newly formed trabecular, which were similar to one of the surrounding bone, were seen at the 42nd day of the experiment. On the other hand, the Low claium diet group showed at the 56th day of the experimen t that the osteoporotic findings looked weak, irregular trabecular, and also large bone marrow space were observed clear ly. As a result of the above experiment, it is said that the healing of the fracture can be completed for both groups, t he Normal diet group and the Low calium diet group. However, the amount of the newly formed bone wound in the Low calciu m diet group is rather decreased compared to one in the normal diet group and at the same time the healing of the fractu re is delayed in the Low calcium diet group. Consequently, for the successful healing of fracture in osteoporosis, it is considered that the management of the etiologic factors of osteoporosis must be preceded. the more study of calcium met abolism and functions of osteoblast and osteoclast needs to proceed on.
Animals ; Bone Marrow ; Calcium* ; Diet ; Hand ; Mandibular Fractures* ; Osteoblasts ; Osteoclasts ; Osteoporosis ; Radiography ; Rats* ; Wound Healing* ; Wounds and Injuries*

Radiation sensitivity data was generated for two human cancer cell lines(KB, RPMI 2650) and human primary gingival fibroblast was tested three times using a viable cell number counting with a hemocytometer, MTT(3-[4,5-dimethylthiazol 2-yl]-2,5-dipheny tetrazolium bromide) assay, and LDH(Lactate dehydrogenase) assay. Single irradiation of 2, 4, 6, 10, 15, 20 Gy were aplied to the tumor cell lines and the primary cultured gingical fibroblast. The two fractions of 4 Gy an d 10 Gy were seperated with a 4 hour time interval. The irradiation was done with 241.5 cGy/min dose rate using 137 Cs MK cell irradiator at room temperature. The obtained results were as followed: 1. There was significantly different viable cell numbers as the amount of radiation dose on the tested cells were cell number counted with a hemocytometer, In fractions, there were more viable cells remaining, 2. Phase-contrast microscopically, radiation-induced morphologic changes were pronounced on the tumor cells, however, a lmost no differences on the gingival fibroblast. 3. There was significantly different absorbance at 2 Gy on RPMI 2650, 4 Gy on KB and GF in MTT assay. In fractions, the absorbance was significantly higher on KB. 4. THe level of extracellular LDH activity in the experimental group was significantly higher in the 2-4 Gy than the co ntrol group. 5. The total level of extracellular and intracellular LDH activity was decreased as increased amounts of radiation dose was applied.
Cell Count ; Cell Line, Tumor* ; Fibroblasts ; Humans ; Radiation Tolerance*