Survivin is closely related to biological behavior of multiple cancers. Significant research progresses have been made in determining Survivin's expression and functional mechanisms in tumor cells. Survivin has been used in identifying tumor markers, predicting prognosis, investigating malignant behaviors and other aspects of cancer research.

Objective To establish CFPAC-1 cell lines deficient in CDC25B2 by recombinant lentivirus, and to investigate the role of this gene. Methods After CFPAC-1 cells were transduced with recombinant lentivirus producing CDC25B2 siRNA, stably transduced cells with green fluorescent protein were selected by flow cytometer. The mRNA and protein expression of CDC25B2 was examined by RT-PCR and Western blot analysis. The effect of the lentivirus on the cell proliferation, cell cycle, clone-forming, migration and invasion ability was analyzed by MTr method, flow cytometer, plate clone-forming assay and Transwell chamber method respectively. Results CDC25B2 siRNA knocked down CDC25B2 expression in CFPAC-1 cells significantly. The silencing efficiency of siRNA transduction by recombinant lentivirns was very high. Proliferation, cloneforming, migration and invasion ability of human pancreatic cancer cell line CFPAC-I were significantly in-creased, while cell cycle was not affected. Conclusion CDC25 B2 plays an important role in cell proliferation, clone-forming, migration and invasion of pancreatic cancer. This research provides experimental evidences for targeting CDC25B2 in gene therapy against pancreatic cancer.

Objective To evaluate the therapeutic efficiency and side effect of concurrent radiotherapy and weekly chemotherapy with low-dose eisplatin(DDP) and 5-fluorouracil(5-FU) for advanced cervical canc-er. Methods 73 patients with advanced cervical cancer were randomized into two groups: 37 cases in the chemo-radiotherapy group received radiotherapy combined with DDP 40 mg and 5-FU 500 mg weekly for 6-7 weeks. 36 cases in the only radiotherapy group received radiotherapy alone. Radiotherapy was given with conven-tional fraction,the total tumor dose was 50 Gy. Results The rate of overall response and three-year survival and local recurrenc and distant metastasis for the chemo-radiotherapy group and the only radiotherapy group were 97.30%, 89. 19%, 8. 11% and 10. 81%, and those for the only radiotherapy group were 77.78%,66. 67% ,30.56% and 33.33%. The difference of the four groups was statistically significant ( P <0. 05). The rate of bone marrow inhibition and reaction of digestive tract in chemo-radiotherapy group were higher than those in simple radiotherapy group, but most reactions were grade 1 and grade 2, without statistically significant dife-renee between two groups. Conclusion Concurrent radiotherapy and weekly chemotherapy with low-dose cis-platin and 5-fluorouracil for advanced cervical cancer can significantly improve local control rate and survival rate, and have slight side effects.

Objective To investigate the expression and clinical significance of Matriptase and HAI-1 in bladder transitional cell carcinoma( BTCC), and to explore their relationship with the clinicopathologieal pa-rameters of BTCC. Methods Expressions of Matriptase and Hal-1 protein in specimens of BTCC and normal bladder tissues were detected by SP method of immunohistochemistry. The results were analyzed in relation to the clinicopathological data. Results The expression of Matriptase and HA1-1 were observed in both normal and tumor tissues of bladder;The protein levels of Matriptase and HAI-1 were significantly higher in normal bladder tissues as compared with BTCC tissues ( P < 0. 05 ) ; a progressive decrease in the protein levels of Hal-1 was observed with increasing of tumor grades (r = -0. 634,P < 0. 001) and clinical stages (r=-0. 521,P < 0. 001 ) ; the protein level of Matriptase was decreased with tumor grade increasing, and was significantly higher in high-grade tissues as compared with moderate and low-grade tissues( P < 0. 05 ) ; Matriptase protein levels showed no significantly difference between different tumor clinical stages ( P > O. 05 ). The correlation co- efficient of Matriptase and HAI-1 in normal tissues was 0. 772(P <0. 001 )and 0. 546 in tumor tissues of blad- der( P < 0. 001 ). Conclusion The abnormal and decreased expressions of Matriptase and HAI- 1 may be im- portant events during the genesis and progression of BTCC in humans; Hal-1 may be used as a new parameterfor assessing the malignancy and prognosis of BTCC; Matriptase protein in BTCC is related with differentiationpromotion, and may be an indicator of differentiation. The correlation between Matriptase and Hal- 1 decreasesin BTCC. The imbalance between HAI-1 and Matriptase may be contributed to the progression of BTCC.

Objective To approach the therapeutic effects and complication of hyperfractionated radio-therapy for nasopharyngeal carcinoma. Methods From January 2000 to November 2002,283 patients with na-sopharyngeal carcinoma were treated with hyperfraetionated radiotherapy. For hyperfractionated radiotherapy,γ-ray from 60Co was performed and two fractions of 1.2 Gy were given daily,with an interval of≥6 h,5 days per week to a median dose of 72 Gy for nasopharynx and 68.4 Gy for neck. For some patients, carboplatin or cispla-tin and 5-fluorouracil were transfused. Results The incidence rate of≥3 grade acute mueositis was 12. 4%.The complete remission rate after treatment was 93.6%. The 1,2 and 5 year survival rate was 93.6% ,82. 7%and 60. 1% respectively. Conclusion patients treated with hyperfractionated radiotherapy have good tolerance and therapeutic effect. It provids a treatment means for nasopharyngeal carcinoma. But it must be validated by the large-scale clinical trials.

Early and non-invasive hematological test in breast cancer is always hot topic. In recent year, enzyme, cytokine, glycoprotein, carcinoembrynoic antigen (CEA), prolactin and oncogene protein are studied more, among them CA15-3 and CEA have important clinical value. Looking for hematological test markers which have high sensitivity and speciality is important work.

Objective To investigate the expression and significance of hypoxia inducible factor-1 alpha(HIF-1α)and glucose transporter protein 1(GLUT-1)in endometrial carcinoma.Methods Immunohistochemical(PV-9000 two-step)method was applied to detect the expression of HIF-1α and GLUT-1 in 36 cases of endometrial carcinoma,18 cases of atypical hyperplasia,12 cases of complex hyperplasia,15 cases of simple hyperplsia,atypical hyperplasia and endometrial carcinoma were 6.7%,25.0%、38.9%and 77.8%.Compared expression of HIF-1αin endomelrial adenocarcinoma with those in endometrial simple hyperplasia,complex hyperplasia and atypical hyperplasia tissue,differences were statistically significant(P＜0.05).The positive rates of GLUT-1 in endometrial simple hyperplasia,complex hyperplasia,atypical hyperplasia and endometrial adenocarcinoma were 0,8.3%,44.4%and 83.3%.Compared expression of GLUT-1 in endometrial adenocarcinoma with those in endometrial simple hyperplasia,complex hyperplasia and atypical hyperplasia tissue,differences were statistically significant(P＜0.05).According to Spearman Correlation analysis,there was positive correlation between HIF-1α and GLUT-1(r=0.559,P＜0.01).Conclusion The positive rates of HIF-1α and GLUT-1 gradually increase in endometrial simple hyperplasia,complex hyperplasia,atypical hyperplasia and endometrial carcinoma,which indicates HIF-1α and its taget gene GLUT-1 play a very important role in the development of endometrial carcinoma.The expression of GLUT-1 can be enhanced by HIF-1α.

The immune tolerance or immune escape plays important roles in the genesis and progression of malignant gynecological neoplasms.Human leukocyte antigen G(HLA-G)is an important member of major histocompatiblity complex class Ⅰ.HLA-G seems to affect almost every aspect of human immunity.It can inhibit immunological function,and play an important role in immune escape of malignant gynecological neoplasms.

Objective To assess the clinical value of multi-slice helical CT perfusion on the post-operative follow-up of glioma.Methods CT perfusion image was applied to 32 patients with glioma after operation.Various perfusion parameters,include cerebral blood volume(CBV),permeability surface(PS),relative cerebral blood volume(rCBV)and relative permeability surface(rPS)were measured on recurrent area,nonrecurrent area and normal cerebral area respectively.SPSS 12.0 statistical software was used.Independent t test was used to compare the differences.Results In the recurrent group(16 cases),the value of CBV in creased in 12 cases and was normal in the other 4 cases.The value of PS increased in all the cases of recurrent group.The value of rCBV exceeded 2.6 in 10 cases,among which the biggest value of rCBV was 4.01.The mean value of rCBV was 2.67 and the mean value of rPS was 6.32;In the non-recurrent group(16 cases),the value of CBV was nearly normal in 7 cases,increased in 2 cases and decreased in the remaining 7 cases.The value of PS was nearly normal in 11 case,decreased in 3 cases and increased in the remaining 2 cases.The values of rCBV were all less than 2.6 and the biggest value of rCBV was 2.14.The mean value of rCBV was 0.99 and the mean value of rPS was 1.42.The statistic significance was obtained for all the parameters when compared the recurrent group with the non-recurrent group(P＜0.01)and with the normal cerebral area(P＜0.01).When compared the non-recurrent group with the normal cerebral area,none of the parameters had statistic significance(P＞0.05);The statistic significance was obtained for rCBV and rPS when compared the recurrent group with the non-recurrent group(P＜0.01).Conclusion CT perfusion image is valuable in determining glioma recurrence after operation.

Caveolin-1 is a prominent member of the caveolin family.Caveolin-1 may have closely correlation with some carcinoma' s origination,development,infihration,transference and drug tolerance of tumor cell.But this function has its specificity of cell type and organ.Caveolin-1 can have different functions in formation and progress of tumor cell.Because different thyroid tumors have different structures and growth patterns ,as well as different biological behavior.Expressions of Caveolin-1 have significant differences in thyroid carcinoma with different histological type and differentiation grade.Research on expression of caveolin-1 in different types of thyroid carcinomas and its physiological characteristics play roles in evaluating prognosis of patient with thyroid carcinoma.