1.A Clinical Study of Lipi Huatan Jiangzhi Tablet for Hyperlipemia
Renxian LI ; Guopu LIU ; Yihong ZENG ; Wensheng XIONG ;
Journal of Guangzhou University of Traditional Chinese Medicine 1999;0(02):-
To investigate the therapeutic effects of Lipi Huatan Jiangzhi Tablet(LHJT),80 cases of hyperlipenda were landomly allocated to LHJT group(50) and lypanthy group(30) and single-blindedtrial was applied.Blood lipids(total cholesterol,triglyceride,high density lipoprotein,apoprotein A andapoprotein B),blood theology and free radicals(lipid peroxide, superotide dismutase and glutathionesuperokidase) were observed.The results showed that the regulatory effect on lipid medslism in LHJTgroup was better and more stable; blood theology, free radicals and the main symptoms were more improved as comapared with lypanthy group. Meanwhile,the total effective rate of LHJT for patients withspleen-deficiency and phlegm-stagnation syndrome was higher than that with other TCM syndromes(P
2.In-Vitro Culture and Plant Regeneration of Morinda officinalis How
Hong HE ; Sheng'E XIAO ; Jianchun XIAN ; Honghua XU ;
Journal of Guangzhou University of Traditional Chinese Medicine 2000;0(04):-
The sterile stem tips, nodular and non-nodular stem of Morinda officinalis How were cultured in vitro. The results showed that the most suitable procedure for sterilization of explants was soaking into 0.1% HgCl 2 solution for 10 to 15 minutes after pretreating with 70% ethanol for 30 seconds. MT culture medium with BA was effective to induce direct shooting of stem tips and nodular stem, the shooting rate of nodular stem being 97.5% . The optimal BA concentration was 1 mg?L -1 , the shooting rate would decrease when the concentration of BA increased. As for the induction of rooting, MT culture medium adding with 0.2 to 0.5 mg?L -1 NAA was optimal, the rooting rate being over 80.0% .
3.In-Vitro Culture and Rapid Propagation of Aloe vera L.Var.chinensis(Haw.)Berger
Hong HE ; Chunlai LIU ; Sheng'E XIAO ; Honghua XU ; Binbin FANG ; Liyi LIANG ;
Journal of Guangzhou University of Traditional Chinese Medicine 2001;0(01):-
Objective To find out technological evidence for the rapid propagation of Aloe vera L.var.chinensis (Haw.)Berber. Methods The technique of plant tissue culture was used to study the rapid propagation of Aloe vera L.Var.Chinesis(Haw.)Berger.The shoot growing from the underground stem of Aloe vera L.Var.chinensis(Haw.)Berger was taken as the explant to induce fascicular bud. Results The optimal culture medium was MT.MT culture medium added with 6-benzylaminopurine(BA) was effective to induce the shoot formation and increase the direct shooting rate.The optimal BA concentration for the induction and formation of shoot was 2 mg?L -1 ,the shooting rate being 100%.As for the induction of rooting,MT culture medium adding with 0.5 mg?L -1 naphthalene acid was optimal,the rooting rate being 100%. Conclusion The culture of Aloe shoot growing from the underground stem,proper culture medium and BA concentration are important for the rapid propagation of Aloe.
4.Influences of Qushang Tablet on Blood Rheology in Rats with Traumatic Blood-Stasis Syndrome and Its Toxicological Study
Guangling ZHONG ; Weiliang CHEN ; Yanping CHEN ; Meixiang YANG ; Chang'An QU ;
Journal of Guangzhou University of Traditional Chinese Medicine 1999;0(02):-
Objective To study the blood_flow_activating and blood_stasis_removi ng actions of Qushang Tablet (QT) and its safety. Methods Thirty rats were randomly allocated to Group A (normal control), Group B (models control) and G r oup C (treated with QT). Rat models with traumatic blood_stasis syndrome were in duc ed by the bruise of falling iron hammer. Effects of QT on blood rheology of rat model were studied. Acute and chronic toxic effect of QT was also studied. Res ults QT had obvious effect on blood rheology of rat model and could improve th e concentration, viscosity, aggregation and coagulability of blood. No acute and chronic toxic effect of QT was found. Conclusion QT exerts obvious blood_fl ow_activating and blood_stasis_removing actions and is safe to be applied in cli nic.
5.Effects of Shengjiang Capsule on Gastric Evacuation in Rats
Weifeng SUN ; Tingli MAO ; Zhe DONG ; Lingbo SHI ; Ruzhenzhan GAO ;
Journal of Guangzhou University of Traditional Chinese Medicine 2001;0(03):-
ive] To observe the effects of Shengjiang Capsule (SC) on gastric evacuation and to study its mechanism. [Methods] Eight-week-old SD rats were randomly allocated to five groups. Group A served as normal control, and Group B were treated with high dosage of SC, Group C with low dosage of SC, Group D with Zhike Baizhu Decotion and Group E with cisapride. After ten days of treatment, gas tric evacuation of rats was detected by isotope tracing method, erythrocyte-acetycholinesterase activity by micro-hydroxylamine method and plasma motilin (Mot) and somatostatin (SS) levels by radioimmunoas-say. [Results] Thirty-minute gastric evacuation rate was (51.44?.38)%, (40.82 + 7.24)% and (40.22?.16)% in Group B, Group C and Group D respectively, which was higher than that in Group A [ (33.18?.32)%]. Erythrocyte-acetycholinesterase activity, Mot and SS levels were (0.856?.128) umol/h, (124.26?5.94) ng/L and (39.42?.96) ng/L, and (0.726?.164 ) umol/h, (119.86 ?9.38) ng/L and (38.33 ?.64) ng/L in Group B and Group C respectively, which were higher than those in Group A [ (0.576 ?.150)/umol/h, (91.28 + 26.84) ng/L and (28.22 ?7.68)ng/L]. [Conclusion] SC can promote gastric evacuation, and its mechanism may be re lated to the increase of the cholinergic nerve function and plasma Mot and SS levels.
6.Effect of Aromatic Herbs for Resuscitation on NO Content and Expression of NOS in Brain Tissue of Rats with Cerebral Ischemia and Reperfusion Injury
Ci'An ZHANG ; Yamin LIU ; Qiuying XU ; Shengquan PENG ;
Journal of Guangzhou University of Traditional Chinese Medicine 1999;0(02):-
[Objective] To investigate the effect of aromatic herbs for resuscitation such as Moschus and Borneol on nitric oxide (NO) content and expression of endothelial NO synthetase (eNOS) and inducible NO synthetase (iNOS) in brain tissue of rats with cerebral ischemia and reperfusion injury. [Methods] Forty rats were allocated to 4 groups: mimic operation group (Group A), model group (Group B), herbs treatment group (Group C) and nimodipine treatment group (Group D). Rat model of cerebral ischemia and reperfusion injury was established by Pulsinelli 4VO method. NO content in brain tissue was determined by nitrate reductase method. Expression of eNOS and iNOS in micrangium and iNOS in neurons was measured by semi-quantitative analysis. [Results] NO content was decreased and approached normal level in Group C and Group D (P 0.05 as compared with Group A). Aromatic herbs with action of inducing resuscitation increased the expression of eNOS in micrangium and decreased the expression of iNOS in micrangium and neurons, the differences being significant as compared with those in Group B ( P
7.Therapeutic Effect of Resuscitation-inducing Aromatic Herbs for Acute Ischemic Stroke
Yamin LIU ; Ci'An ZHANG ; Qiuying XU ; Peizheng LIN ; Shengquan PENG ;
Journal of Guangzhou University of Traditional Chinese Medicine 2001;0(03):-
To investigate the effect of treatment based on syndrome differentiation combined with resuscitation inducing aromatic herbs for acute ischemic stroke (AIS).Forty four cases of AIS were randomly allocated to two groups. Twenty cases of Group A were treated with treatment based on syndrome differentiation combined with Tongqiao capsule(each capsule containing Moschus 0 04?g and Borneolum 0 1?g)and twenty four of Group B with treatment based on syndrome differentiation. Therapeutic effect were observed in the second week after medication. The total effective rate was 95% in Group A and 91 6% in Group B, the difference being not significant. However, the difference of markedly effective rate (80 0% in Group A and 54 2% in Group B) was significant (P
8.Content Determination of Brucine and Strychnine in Tongbiling Tablet by Reversed-phase HPLC
Ke'Er HUANG ; Xuehong KE ; Jifan CHEN ;
Journal of Guangzhou University of Traditional Chinese Medicine 1999;0(02):-
[Objective] To establish a method for the determination of brucine and strychnine contents in Tongbiling Tablet. [Methods] Reversed-phase HPLC conditions were: Nucleodur C18 Gravity column (125 mm ? 4.6 mm, 5?m), mobile phrase being methanol-water (60:40) with 0.5 g potassium dihydrogen phosphate and 1.0 g dodecyl sodium sulfouate and 0.5 mL triethylamine in 1 000 mL mixture (adjusting pH to 4.5 with acetic acid) , detecting wavelength at 254 run and a flow rate of 1.0 mL/min at room temperature. [ Results ] The average recovery of brucine was 97.69 % ( RSD = 2.03%) and that of strychnine 97.93% ( RSD = 2.61%). [Conclusion] This method is efficient and can be used for the quality control of Tongbiling Tablet.
9.Inhibitory Effect of Danggui Decoction on Passive Cutaneous Anaphylaxis in Mice
Journal of Guangzhou University of Traditional Chinese Medicine 2000;0(04):-
【Objective】To explore the mechanism of Danggui Decoction (DD) in counteracting allergic reaction bymeans of passive cutaneous anaphylaxis (PGA) test.【Methods】Among fifty NEH mice, ten were used to prepareantiserum and the rest forty were randomized into 4 groups: normal control group (A), clarityne group (B), low-doseDD group (C) and high-dose DD group (D) . PGA was induced by intraperitoneal injection of antiserum in the fourgroups and the mice were treated with gastric infusion simultaneously. After the last feeding, antigen attack wasconducted. Inhibitory rate of PCA in four groups were measured by colorimetric analysis after treatment. 【Results】High-and low-dose DD and clarityne could inhibit PCA in mice (P0.05).【Conclusion】The anti-allergic effect of DD may be associated with the inhibition of type Ⅰ allergic reaction.
10.Effect of Yiyiren Gancao Tang in Preventing and Treating Condyloma Acuminatum: An Observation of 68 Cases
Daoshun SUI ; Min HE ;
Journal of Guangzhou University of Traditional Chinese Medicine 2000;0(04):-
【Objective】To observe the effect of Yiyiren Gancao Tang (YGT) in preventing the recurrence of condylomaacuminatum (CA) after microwave therapy and to explore its influence on relative immunological indexes. 【Methods】Sixty-eight cases of CA were randomized into two groups: 34 cases (Group A) were treated with YGT and other 34 withplacebo decoction (Group B) after microwave therapy. The recurrence rate in the two groups were observed; seruminterleukin-2 (IL- 2) level and subtypes of T lymphocyte counting in peripheral blood were detected with double antibodysandwich immunoassay and rosette formation test before and after treatment.【Results】The recurrence rate was 20.5% inGroup A and 47.0% in Group B (P.05).【Conclusion】YGT can reduce the recurrence of CA, improve the immune function and is practical in clinics.