Objective　To explore the potential hazards of organic pollutants in chlorinated drinking water on human reproduction.Methods　The reproductive toxicity of the organic pollutants extracted by solid phase extraction technique from chlorinated drinking water in a city,was determined by the co-culture of sertoli cells and spermatogenic cells.Results　No significant variation of the rate of detached spermatogenic cells was noticed compared with the control rats.The organic extracts stimulated the secretion of lactate from sertoli cells of testis in rats.Conclusion　The organic extracts of chlorinated drinking water revealed potential reproductive toxicity.

Objective　To findout a good method for malignant transformation of human bronchial epithelial cells 16HBE cell induced by anti-benzo (a) pyrene-trans-7,8-dihydrodiol-9,10-epoxide (anti-BPDE) using the objective and simple index:colony forming frequency of cells in semisolid agar culture medium,and to establish a best model of malignant transformation of 16HBE cells.Methods　The tests on viable rate and colony formation of 16HBE cells were carried out to determine the exposure doses of anti-BPDE.The l6HBE cells were treated once or several times by anti BPDE at different doses and the transformed foci were observed and assessed at the different stages during the period of the whole experiment.The malignant features of transformed 16HBE cells with a good dose-response relationship were identified by the method of semisolid agar culture.The anti-BPDE induced colony forming frequencies of 16HBE cells in semisolid agar medium in each dose group were statistically compared.Results　The best method for malignant transformation of 16HBE cells induced by anti-BPDE was that the 16HBE cells were intermittently treated several times with anti-BPDE at doses of 0.1,0.5,1.0 and 2.0 μmol/L respectively and were inoculated for 15 generations,the related colony forming frequencies were 2.0‰,5.5‰,7.0‰ and 10.5‰respectively with a significant dose-response relation ship and a good linear correlation (r=0.9741,P＜0.05).Conclusion　The research infered that the malignant transformation of human bronchial epithelial cell-16HBE cells could be induced successfully by anti-BPDE,a suitable model could be established by anti-BPDE also.

Objective　To explore the mechanism of male reproductive toxicity of bisphenol-A.Methods　The morphological characteristics of seminiferous tubule,vimentin filaments and p53 gene expression in sertoli's cells of adult SD rats orally exposed to 0.5% bisphenol-A were observed and analyzed by histo-chemical and immuno-histochemical methods.Results　After 2-week-exposure to 0.50% bisphenol-A,the disattachment between sertoli's cell and spermatogonia,spermatogonia arranged in disorder and displacement of spermatogonia away from the basement membrance of seminiferous tubules as well as the flocculated chromatins of nuclei in sertoli's cells and spermatogonia were observed.Fourfold increase of p53 expression in nuclei of spermatogonia and leydig's cells and the collaps of vimentin filaments in sertoli's cells were also found.Conclusion　The results suggested that the disattachment between sertoli's cells and spermatogonia,might be associated with the collaps of vimentin filaments in nuclei of sertoli's cells and the increase of p53 expres sion in nuclei of sertoli's cells and spermatogonia,which might be one of the important mechanisms of reproductive toxicity of bisphenol-A.The increase of p53 expression in nuclei of leydig's cells might predict the potential anti-androgenicity of bisphenol-A

Architectural environment is being paid more and more attention, especially the indoor air environment. With the development of architecture, the problem caused by sick building will become a hotspot of research. There are many measures to lower the concentration of indoor toxic gases and avoid sick building syndrome. This paper concretely analyzes the kinds of contaminants affecting the indoor air quality expounds the measures to improve indoor air quality, and points out the research direction of purifying indoor air.

Three aspects of advances in study on the carcinogenicity of alga toxins are reviewed in this paper. Water pollution by alga toxins has become a worldwide environmental problem, and special attention has been paid to the potential carcinogenicity of microcystins, the main alga toxin. Results from the study on structure_activity relationship, short_term screening assay in vitro and long_term rodent carcinogenesis bioassay all showed that alga toxins possessed tumor_promoting activities. It suggested that inhibition of protein phosphatase activities, induction of expression changes of cell proliferation_related genes, dysregulations of signal transduction and cell proliferation might be the mechanisms of the carcinogenesis effect of alga toxins.

Objective To explore a method for determination of dissolvable zinc salt in deodorizing cosmetics. Methods The contents of dissolvable zinc salt in deodorants extracted by pure water were detected by flame atomic absorption spectrophotometry. Results The correlation coefficient of the standard curve was more than 0.999. The detection limit, precision and recovery rates of the method were 8.2?10-3 ?g, 1.26% and 97.0%～98.5% respectively. The lowest detection limit of zinc salt was 2.7?10-2 ?g. No other interfering factors were found during detection process. Conclusion This method could meet the requirements of hygienic physico_chemical detection for cosmetics.

Objective To explore an easy, rapid and suitable method for determination of cyanide with lower concentration in quality_controlled water sample. Methods To overcome the shortcomings in determination of low concentration cyanide in water by iso_niacin_pyrazolone spectrophotometry and pyridin_barbituric acid spectrophotometry, the iso_niacin_barbituric acid spectrophotometry was studied for determining the lower concentration cyanide in water, the data obtained were compared with those obtained from the standard method iso_niacin_pyrazdone spectrophotometry. Results The results showed no statistically significant differences between these two methods. The precision, accuracy and other indexes all accorded with the analysis criteria. Conclusion The iso_niacin_barbituric acid spectrophotometry was easier to operate, more rapid and lower cost, the conditions of colorimetry were easier to be controlled compared with those of iso_niacin_pyrazolone spectrophotometry.

Objective To study the characteristics of the variations of formaldehyde concentration in indoor air of newly decorated living rooms. Methods The formaldehyde concentrations in indoor air of newly decorated rooms were determined with model 4160_2 formaldehyde analyzer during the different periods after decoration. Results At the 10th minute after closing all the windows and doors in the newly decorated rooms, the formaldehyde concentrations of indoor air exceeded the standard nearly as high as 2 times, reached 51% of the peak concentration at the 30th minute, and reached the peak concentration (0.652 mg/m 3) at the 420th minute, then decreased gradually with the prolongation of periods after decoration and accorded with the sanitary standard in the 4th month after decoration. The whole study was carried out at the indoor air temperature of 27.8～29.9 ℃. Conclusion The indoor air pollution by formaldehyde was serious in newly decorated living rooms. The ventilation should be enhanced during the first 4_month period after decoration, so that the formaldehyde concentration could be decreased gradually.

Objective To study the relationship between the contents of arsenic, chromium and lead in the flowers of Robinia pseudoacacia L and soil sown to them in urban area of Qingdao. Methods The flowers of Robinia pseudoacacia L and soil, where they grew, were sampled for analysis in different quarters in urban area of Qingdao in May,2001. Results The sanitary qualities of the soil samples all maintained Grade 1, no one of the samples exceeded the related national sanitary standards for the contents of arsenic, chromium and lead in soil. Based on the national standards for As, Cr and Pb in food, the As contents in the sampled flowers of Robinia pseudoacacia L were all below the related national standard. The Cr contents in the flower samples exceeding the standard were only found in industrial quarter. The Pb contents in all flower samples exceeded the standard. Conclusion The low contents of Cr and Pb in flower samples were associated with those in the soil sown to Robinia pseudoacacia L and other environmental factors in a certain degree.

Objective To explore the effects of lower concentrations of rare earth elements in environment on human health. Methods The representative well water samples, soil samples and vegetable samples were selected near the rare earth ore and non_rare earth ore. The contents of La and other 15 kinds of rare earth elements were determined by ICP_MS. Results The total contents of rare earth elements were 9.18 ?g/L for well water samples, 869.73 ?g/g for soil samples, 6 370.35 ng/g (dry weight) for vegetable samples respectively near the countryside of rare earth elements ore, which were all higher than those in non_rare earth elements. Conclusion Human health might be affected by rare earth elements in drinking water and foods in rare earth ore.