Objective To explore the influence of electroacupuncture preconditioning on cognitive function and adenosine monophosphate-activated protein (AMPK) signaling pathway in depression model rats after electroconvulsive therapy (ECT). Methods Totally 75 male sprague-dawley rats were randomly divided into normal control group (groupⅠ), model group (group Ⅱ), electroconvulsive therapy group (group Ⅲ), electroacupuncture + electroconvulsive therapy group (groupⅣ), false electroacupuncture + electroconvulsive therapy group (group Ⅴ), with 15 rats in each group. GroupⅡ ～ Ⅴ were treated by chronic unpredictable mild stress (CUMS) to establish model of depression. After successful modeling, all rats in the four groups were anesthetized with 100 mg/kg by intra-peritoneal injection of propofol. Group Ⅱ was treated by anesthesia without electroconvulsive therapy, the other three groups were treated by electroconvulsive therapy. Group Ⅳ was treated by electric acupuncture at Bai hui (GV20) and Yin tang (GV29) acupoint for 30 minutes before ECT.Group Ⅴ was treated by false electric acupuncture at 5 mm next to Bai hui (GV20) and Yin tang (GV29) acupoint for 30 minutes before ECT. The rats were kept adaptively feeding for seven days, then the experiment started (the first day of the experiment). Morris water maze test (positioning navigation experiment and space exploration experiment) was carried out on the 1 stday, the 28 thday and the 40 thday of the experiment respectively. On the 6 thday, the 33 rdday and the 45 thday of the experiment, 5 rats in each group were randomly selected. After the rats were sacrificed, the expressions of AMKP and phosphorylated AMKP (p-AMKP) in the hippocampus were determined by Western blot method. Results Compared with group Ⅰ, the escaping latency and the swimming path extended obviously and the crossing platform number decreased significantly on the 32 ndday in group Ⅱ to Ⅴ and the 44 thday in group Ⅱ, Ⅲ, Ⅴ. Compared with the result on 5 thday, the escaping latency and the swimming path extended obviously and the crossing platform number decreased significantly on the 32 ndday in group Ⅱ to Ⅴ and the 44 thday in group Ⅱ, Ⅲ, Ⅴ. Compared with group Ⅲ, the escaping latency and the swimming path shortened obviously and the crossing platform significantly increased on the 44 thday in group Ⅳ, and there were significant differences (P ＜0. 05). Compared with group Ⅰ, the expressions of AMPK and p-AMPK in hippocampus significantly reduced on the 32 ndday in group Ⅱ to Ⅴ and the 44 thday in group Ⅱ, Ⅲ, Ⅴ. Compared with the results on 5 thday, the expressions of AMPK and p-AMPK in hippocampus significantly reduced on the 32 ndday in group Ⅱ toⅤ and the 44 thday in group Ⅱ, Ⅲ, Ⅴ. Compared with groupⅢ, the expressions of AMPK and p-AMPK in hippocampus increased obviously on 44 thday in groupⅣ, and there were significant differences (P ＜0. 05). Conclusion Electroacupuncture preconditioning can improve the learningand memory ability of depression model rats after electroconvulsive therapy, and has a good anti-depressant effect. The mechanism may be related to the up-regulation of the expression of AMPK signaling pathway in the hippocampus.

Objective To investigate the effect of insulin pump combined with dipeptidyl peptidase-Ⅳ (DPP-Ⅳ) inhibitor on the treatment of newly diagnosed type 2 diabetes mellitus (T2 DM). Methods Totally 51 newly diagnosed patients with T2 DM were randomly divided into experimental group (insulin pump combined with DPP-Ⅳ inhibitor) and control group (insulin pump alone). After 15 days of treatment, the clinical effect was compared between two groups. Results After treatment, the blood sugar related indexes in both groups significantly improved (P ＜ 0. 05), and the blood sugar control of the experimental group was significantly better than that of the control group (P ＜ 0. 05). The pancreatic β cell function index, area under curve of C-peptide and insulin resistance index in the experimental group were significantly better than those in the control group (P ＜0. 05). The dosage of insulin, the ratio of patients with blood sugar reaching the standard completely, the time of blood sugar reaching the standard and the increase value of body mass in the experimental group were significantly better than those in the control group (P ＜ 0. 01). The incidence rate of hypoglycemia was 8. 00％ (2/26) in the experimental group and 12. 00％ (3/25) in the control group, and there was no significant difference between the two groups (P＞ 0. 05). During the treatment, no severe hypoglycemic events and liver and kidney function damage occurred in both groups. Conclusion Insulin pump combined with DPP-Ⅳ inhibitor is effective in the treatment of newly diagnosed T2 DM, which can effectively control blood sugar, reduce insulin resistance and weight gain.

Objective To analyze the detection results of apolipoprotein B (ApoB) and serum uric acid (SUA) in patients with non-alcoholic fatty liver disease (NAFLD). Methods A total of150 NAFLD patients admitted to our hospital were selected as study group, and 150 healthy people with physical examination in the same period were as the control group. Low density lipoprotein cholesterol (LDL-C), triglyceride (TG), alanine aminotransferase (ALT), waist-hip ratio (WHR), body mass index (BMI), total cholesterol (TC), ApoB, creatinine (Cr), SUA levels were measured in the two groups. The correlation between SUA, ApoB and NAFLD was analyzed by multiple Logistic regression analysis. At the same time, smoking, coronary heart disease, diabetes and hypertension were compared between the two groups. Results The levels of LDL-C, TG, ALT, WHR, TC, BMI, SUA and ApoB in the study group were higher than those in the control group (P ＜ 0. 05). The levels of Cr in the study group were significantly lower than those in the control group (P ＜ 0. 05). The results of multiple Logistic regression analysis showed that the levels of SUA and ApoB were independently and positively correlated with NAFLD and were independent risk factors for NAFLD. The incidences of smoking, coronary heart disease, diabetes mellitus and hypertension were significantly higher than that of the control group (P ＜ 0. 05). Conclusion The levels of SUA and ApoB are positively correlated with NAFLD. The higher the levels of SUA and ApoB are, the higher the incidence of cardiovascular disease is. Therefore, blood lipid intervention should be carried out in time to control the development of NAFLD.

Objective To investigate the clinical efficacy of video-assisted thoracic surgery (VATS) in the treatment of elderly non-small cell lung cancer (NSCLC). Methods A total of 92 elderly patients with NSCLC admitted to our hospital were randomly divided into control group and observation group, with 46 cases in each group. The control group was treated with classical treatment of thoracotomy plus mediastinal lymph node dissection, while the observation group was treated with VATS. The operation-related indexes, cardiac function [heart rate, (HR), pulse oxygen saturation (SpO2) ]and respiratory function indexes[forced vital capacity (FVC), forced expiratory volume in the first second (FEV1), maximal voluntary ventilation (MVV) ]were compared between the two groups. The incidence of complications, hospital stay and expenses were compared. Results The operation was successfully completed in both groups, and no patients conversed to thoracotomy in the observation group. There were no significant differences in operation time and number of lymph node dissection between the two groups (P＞ 0. 05). Incision length, intraoperative bleeding volume and indwelling drainage time of the observation group were shorter than that of the control group (P ＜0. 01); but no differences were observed in HR, SpO2, FVC, FEV1, MVV of the two groups (P＞0. 05). The above indexes (except for SpO2) at 1 week after treatment of the two groups showed significant differences compared with treatment before (P ＜ 0. 05), and showed significant between-group differences (P ＜ 0. 05). The complication rate of the observation group was lower than that of the control group (6. 52％ vs. 21. 74％, P ＜ 0. 05), and the hospitalization time and medical expenses were (11. 93 ± 2. 57) d, (3. 06 ± 0. 58) ten thousand RMB, which were significantly lower than (13. 62 ± 3. 14) d, (3. 43 ± 0. 75) ten thousand RMB (P ＜ 0. 05). Conclusion Video-assisted thoracoscopic lobectomy (VATS) for elderly patients with NSCLC can not only achieve the same effect as thoracotomy for resection of tumors, but also have the advantages of less impact on cardiac function and pulmonary respiratory function, rapid recovery and less complication, and lower medical costs.

Objective To investigate the effect of dexmedetomidine combined with fentanil on postoperative sleep quality and cognitive function in elderly patients with patient-controlled intravenous analgesia (PCIA). Methods Forty elderly patients underwent thoracic surgery were randomly divided into control group (fentanil group, n = 20) and experimental group C (fentanil plus dexmedetomidine group, n = 20), The control group was mixed 15 μg/kg fentanil and 5 mg tropisetron into100 mL of normal saline in patient-controlled intravenous analgesia pump, and experimental group contained 15 μg/kg fentanil, 1. 0 μg/kg dexmedetomidine and 5 mg tropisetron in 100 mL of normal saline. The pump was withdrawn after 48 h. The total steep time, sleep quality were recorded at 1 d before surgery and on the day of surgery, and at 3 d after surgery. The Mini-Mental Status Examination (MMSE) were recorded at 1 d before surgery and 1, 3, 7 d after surgery, and the incidence of postoperative cognitive dysfunction was observed. Results Compared with control group, the sleep time of experimental group on the surgery day and at 2 d after surgery was longer (P ＜ 0. 05); the sleep quality were significantly higher (P ＜ 0. 05); the MMSE scores of experimental group on the first and third postoperative day were higher (P ＜ 0. 05), and the incidence of POPD was lower than that in the control group (P ＜ 0. 05). Conclusion Dexmedetomidine combined with fentanil administered for postoperative analgesia in elderly patients shows better efficacy in improvement of sleep status, and improve the postoperative cognitive function.

Objective To analyze the clinical efficacy of levocarnitine combined with Yougui Capsule on oligospermia and asthenospermia in male patients and its adverse effects. Methods Eighty male patients with oligospermia admitted to our hospital were randomly divided into study group and control group, with 40 cases in each group. The control group was treated with levocarnitine alone, and study group was additionally given Yougui Capsule. The total effective rate, incidence of adverse reactions, semen quality parameters and changes of sex hormones were compared between the two groups after 3 months of treatment. Results The total effective rate, plasma testosterone (T) level, and follicle stimulating hormone (FSH) in the study group were 87. 50％, (812. 3 ±130. 2) ng/dL, and (9. 1 ± 3. 5) IU/L, respectively, which were superior to 65. 0％, (778. 4 ±113. 2) ng/dL, (8. 0 ± 2. 1) IU/L, in the control group (P ＜ 0. 05). The incidence rate of adverse reactions in the study group was significantly lower than that of the control group (2. 50％ vs.20. 00％, P ＜ 0. 05). The semen quality parameters of the study group were significantly better than the control group (P ＜ 0. 05). Conclusion The combination of levocarnitine and Yougui Capsule can effectively improve the sex hormone level and sperm quality, improve the reproductive function, which have fewer adverse reactions, and is safe and reliable.

Objective To investigate the safety and effect of butorphanol combined with ketorolac tromethamine for postoperative analgesia of patient-controlled intravenous analgesia (PCIA) after radical mastectomy. Methods A total of 60 patients underwent elective radical mastectomy with general anesthesia were randomly assigned into 2 groups, with 30 cases in each group. PCIA was used in both groups and group A was given butorphanol 10 mg, and group B was given butorphanol 10 mg as well as ketorolac tromethamine 60 mg. Visual analog scale (VAS) scores and Ramsay sedative score were recorded at 2, 24 and 48 h after operation, pressing times of analgesic pump within 48 hours, incidence rate of adverse reactions and the overall satisfaction for postoperative analgesia were observed and recorded. Results Compared with group A, the VAS scores at postoperative 24 and 48 hours in group B were decreased, and the pressing times of analgesic pump were significantly lower (P ＜ 0. 05). There were no statistically significant differences in Ramsay sedation score at each time points and the incidence rate of adverse reactions between the two groups (P＞ 0. 05). Compared with group A, the overall patients' satisfaction for postoperative analgesia was higher in group B (P＞0. 05). Conclusion Butorphanol combined with ketorolac tromethamine has better analgesic effect in patients with PCIA after radical mastectomy, with less incidence of adverse reactions, and higher postoperative satisfaction for analgesia.

Objective To explore the effect of early sedative in prevention of febrile convulsion epilepsy in children. Methods A total of 146 children with febrile convulsion epilepsy who were treated in our hospital were selected as the researchobjects. According to the random number table method, they were randomly divided into control group and research group, with 73 cases in each group. The control group was treated with routine treatment such as symptomatic cooling, while the study group was treated with sedatives at the early stage. After 6 months, the clinical symptoms and preventive effects of the two groups were compared. Results After treatment, the incidences of epilepsy, brain injury and mental retardation in the study group were significantly lower than that in the control group (P ＜ 0. 05), and the cure rate in the study group was significantly higher, and recurrence rate was significantly lower than that of the control group (P ＜ 0. 05). Conclusion Sedatives are effective in the treatment of early febrile convulsions and epilepsy in children. It can alleviate the symptoms, effectively prevent the seizures caused by febrile convulsions and other complications, and reduce the recurrence rate.

Objective To explore the clinical efficacy and safety of blind insertion of bronchial occluder for children with one-lung ventilation under the guidance of tracheal catheter obliquely facing right or left. Methods A total of 70 children undergoing right thoracotomy in our hospital were selected as study subjects, and were randomly divided into observation group (trachea tube inclined to right) and control group (trachea tube inclined to left), with 35 cases in each group. The baseline data and related clinical indexes, and the incidence of postoperative complications were compared between the two groups. Results There was no significant difference in baseline data between the two groups (P＞ 0. 05). The blocking time in the observation group was significantly shorter, the rate of one-time success rate was higher, and the incidences of airway edema and hypoxemia after operation were significantly lower than that in the control group (P ＜ 0. 05). Conclusion The adjustment of the opening slope of the tracheal catheter to the right can effectively shorten the total timeconsuming of plugging, improve the one-time insertion success rate, and reduce the incidence of postoperative complications.

Objective To explore the effect of medical polyethylene glycol berberine on clinical outcomes after curettage. Methods A total of 166 curettage patients were divided into study group (n = 80) and control group (n = 86) by the patients' willingness. The two groups were given routine curettage, and the study group were additionally given 3 mL medical polyethylene glycol berberine by uterine cavity injection, while the control group infused no drugs. The postoperative endometrial thickness, menstruation situation were compared, and the re-pregnancy conditions of the patients in control group (n = 20) and experimental group (n = 32) with two-child requirement at follow-up and in one and a half year after follow-up were compared. Results The endometrial thickness in the study group was thicker than that in the control group [ (9. 44 ± 1. 95) mm vs. (8. 65 ± 2. 28) mm, P ＜ 0. 05], the proportion of the endometrial thickness ＜ 8 mm in the study group was lower than that in the control group[27. 5％ (22/80) vs. 38. 4％ (33/8), P ＜ 0. 01]. The ratio of patients with eumenorrhea was lower than that in the control group[ (10％ (8/80) vs. 16. 3％ (14/86) ], but there was no significant difference (P＞ 0. 05). The clinical pregnancy rate of the patients who had pregnancy requirements in the control group was higher than that in the control group [93. 75％ (30/32) vs. 70％ (14/20), P ＜ 0. 05]. Conclusion Medical polyethylene glycol berberine can promote the pregnancy rate. Meanwhile, the operation is simple and has higher compliance rate, which is an effective way in improving clinical outcomes.