The secondary structures of fusion protein pp150/MDBP, including alpha-helix, beta-sheet, turn regions, were analyzed by Garnier-Robson's and Chou-Fasman's methods; the antigenic epitopes of B cells were analysed by using hydrophilicity plot. The results showed that the fusion protein pp150/MDBP might have less alpha-helix, but be rich in beta-sheet and turn regions. The epitopes recognized by B cells may be at 7-56 amino acid residues or adjacent to 137-192 amino acid residues.
Amino Acid Sequence ; Binding Sites ; Cytomegalovirus ; chemistry ; Epitopes ; Humans ; Molecular Sequence Data ; Phosphoproteins ; chemistry ; immunology ; Protein Structure, Secondary ; Viral Fusion Proteins ; chemistry ; immunology ; Viral Matrix Proteins ; chemistry ; immunology

Experimental autoimmune encephalomyelitis (EAE) is an autoimmune disease of the central nervous system (CNS); it serves as a model for the human multiple sclerosis (MS). In mice, EAE is mediated by T cells specific for various myelin basic proteins which migrate from the periphery to the CNS. In search of a way to prevent the induction and progression of EAE, we observed the effects of recombinant immunotoxin IP10-DT390 on blocking or eliminating the active T cells in the EAE model. In this paper is presented an experimental gene therapy-based model in which the mice were made resistant to EAE induction by plasmid DNA encoding recombinant immunotoxin that was injected into the leg muscles of mice. The new immuno-biological construct could selectively impair autoreactive T-cell homing while the duration of clinical signs is shorter, and the new construct would not affect other components of the immune response. These data demonstrated the effectiveness of the constructs in the treatment of EAE and suggested its usefulness in the treatment of other autoimmune diseases.
Animals ; Chemokine CXCL10 ; biosynthesis ; genetics ; therapeutic use ; Diphtheria Toxin ; biosynthesis ; genetics ; therapeutic use ; Encephalomyelitis, Autoimmune, Experimental ; immunology ; pathology ; therapy ; Female ; Genetic Therapy ; Immunoglobulin Fragments ; biosynthesis ; genetics ; therapeutic use ; Immunotoxins ; genetics ; metabolism ; therapeutic use ; Mice ; Mice, Inbred C57BL ; Receptors, CXCR3 ; metabolism ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; therapeutic use ; Recombinant Proteins ; biosynthesis ; genetics ; therapeutic use ; T-Lymphocytes ; immunology ; Transfection

Transgenic chicken and oviduct bioreactor are growing to be one of the hotspot of scientific study in the field of biology. The most successful method of producing transgenic chicken is pseudotyped retrovirus vector system, but no one has reported the production of transgenic chicken by retrovirus system recently in our country. In order to accelerate our study in this field, we introduced the relevant technical methods such as packaging retrovirus and vesicular stomatitis virus G glycoprotein (VSV-G) pseudotyped retrovirus, optimizing the conditions of packaging retrovirus, concentrating VSV-G pseudotyped retrovirus, helper virus assays, and microinjection of retrovirus. Furthermore, we successfully conducted in vivo study for detecting the marker gene EGFP of chicken embryo as well as in vitro study for detecting that gene of chicken embryo myoblast (CFM), thus we have provided an applied technical platform for studies of transgenic chicken in the future.
Animals ; Animals, Genetically Modified ; Chick Embryo ; Chickens ; genetics ; DNA Primers ; Genetic Vectors ; genetics ; Retroviridae ; genetics ; Vesicular stomatitis Indiana virus ; genetics

Selecting reused polysulfone membrane (PSM) dialyzers as research objects, we mainly analyzed quantitatively the adhesion ingredients which obstructed the passage through the membrane, and we investigated the differences of residual contaminants on the surface of PSM in the cases of various reuse times. The results illustrated that after the completion of dialysis, the dialyzer was first cleaned by reverse osmosis (RO) water to remove the protein adsorpted. Then we used 2% sodium hypochlorite (NaClO) solution to soak it, and the glucose, cholesterol and triglyceride adsorpted were dissolved off. Meanwhile, the quantity of most of adsorption gradually increased with the increase of reuse times.
Adsorption ; Adult ; Cholesterol ; analysis ; Equipment Reuse ; Female ; Glucose ; analysis ; Humans ; Male ; Membranes, Artificial ; Middle Aged ; Polymers ; Proteins ; analysis ; Renal Dialysis ; adverse effects ; instrumentation ; Sulfones ; Triglycerides ; analysis

The biological safety of degradable products is a key point in biological evaluation of biodegradable materials. This paper deals with an investigation of the influence of PGLA degradable products on physiologic function of liver and kidney by detecting the "before-after change" of some biochemical indexes with the use of self-control method. PGLA materials of different sizes were implanted into the subcutaneous tissue of Wistar rats and New Zealand rabbits respectively. Also for the purpose of group comparison, only surgical operation was performed on other animal at the same site (control group). The concentrations of urea nitrogen, uric creatinine, blood urea nitrogen (BUN), carbamide (Cr) and serum glutamio pyruvic transaminase (GPT) were measured before and after implantation (from 2 w to 10 w). The results showed the values of urea nitrogen and uric creatinine were significantly increased at 2-3 weeks after implantation (P < 0.01). After 2 weeks of implantation, the concentration of GPT was slightly decreased (P < 0.05). The BUN and Cr were remarkably increased (P < 0.01). But at the 4th week, all the values returned to normal and kept stable thenceforth. All the biochemical indexes in the control animal exhibited no abnormal change after operation. These results indicated: (1) There are no permanent harmful effects of PGLA degradable products on the function of liver and kidney; (2) By means of self-control and measurements of some biochemical indexes in the blood or urea, the method is suitable for evaluating the biological safety of degradable products; (3) The method has the advantages for evaluating the bioaccept ability of degradable products during the life period, it is objective and sensitive, the number of animal can be decreased, the process of dynamic change in vivo can be observed directly, and the effect of degradable products on physical function can be evaluated. Therefore, the method can provide a new approach for biological evaluation of biodegradable materials.
Absorbable Implants ; adverse effects ; Animals ; Biodegradation, Environmental ; Female ; Glycolates ; adverse effects ; chemistry ; Implants, Experimental ; Kidney ; physiology ; Lactic Acid ; Liver ; physiology ; Male ; Materials Testing ; Polyglycolic Acid ; Rabbits ; Rats ; Rats, Wistar

The functional hemocompatibility between fibrinogen (FIG) and a novel vascular stent material (Ti-O film fixed with albumin and heparin) was investigated as follows: (1) Preparing the new biologic material (Ti-O) film; (2) Coating albumin and heparin on the Ti-O film; (3) Testing platelets (PL) adsorption; (4) Determining FIG adhesion number by use of enzyme linked immunoassay (ELISA); (5) Implanting the films from the test group of Ti-O film and from the comparison group of stainless steel (SS) film into the left and right femoral arteries respectively in 4 dogs. It was proved that albumin and heparin were fixed on Ti-O film. After 6 months, the femoral arteries of the dogs were resected. In the test group of Ti-O film coated with albumin and heparin, few PL adhered to the coat, their form did not change, and no thrombus was found by scanning electron microscopy; the result was better than that of plain Ti-O film, and was much better than that of SS film. Ti-O maintained normal transformation condition of FIG, and no C terminal of gamma chain in FIG was revealed. As it is known whether the hemocompatibility of a biomaterial is good depends upon its adsorption of FIG, and Ti-O has excellent reaction on albumin and heparin by chemical processes. In this study, the Ti-O film coated with albumin and heparin further reduced the absorption of FIG and PL when compared against the plain Ti-O film. So the Ti-O film coated with albumin and heparin has the insistent and permanent anticoagulant character.
Albumins ; chemistry ; Animals ; Coated Materials, Biocompatible ; pharmacology ; Dogs ; Fibrinogen ; chemistry ; Heparin ; chemistry ; Materials Testing ; methods ; Prosthesis Design ; Stents ; Surface Properties ; Titanium ; chemistry

Based on the study of extraction of DNA using carboxyl-coated Fe3O4, here we provide a rapid, high efficient, safe and economical method. Using fluorescent dye-Sybr Green I, the quantification of double-strand DNA exhibits the advantages of high sensitivity,good repeatability and convenience.
Carbon Dioxide ; chemistry ; DNA ; blood ; isolation & purification ; Ferric Compounds ; chemistry ; Fluorescent Dyes ; Humans ; Magnetics ; Nanoparticles ; Organic Chemicals ; Particle Size ; Staining and Labeling

The control arithmetic is proposed for axial blood pump driven by extracorporeal alternating magnet field based on ventricular work. According to health physiological parameters, the control model is verified by calculation and experiment. The control model used for driving blood pump is derived from the relationship of natural heart and artificial heart. By comparison with others, the control arithmetic based on ventricular work is more helpful in appraising the property of left ventricular aided device (LVAD), in meeting human body's natural need, and in effective use of motor for avoiding motor running at high speed and hence preventing the blood from the destruction caused by a blood pump at high temperature.
Algorithms ; Cardiac Output ; physiology ; Computer Simulation ; Heart Ventricles ; Heart-Assist Devices ; Hemorheology ; Humans ; Models, Cardiovascular ; Ventricular Function, Left ; physiology

A detailed three-dimensional nonlinear finite element model of lumbar segment L3-L5 was developed to investigate the influence of vibration on the components of human spine. The results show that the vibration effects of different spinal components are not exactly the same, and the stress near the posterior region of L4-L5 annulus is higher than that of its anterior region. The vibration exerts a great influence on the facet joint of L4-L5 segment. The changing amplitudes of stress and deformation of spine reduce by 50% on the condition that the damping ratio is 0.08.
Biomechanical Phenomena ; Cadaver ; Computer Simulation ; Finite Element Analysis ; Humans ; Lumbar Vertebrae ; physiology ; Models, Biological ; Nonlinear Dynamics ; Stress, Mechanical ; Vibration ; Weight-Bearing ; physiology

Using the method of detrended fluctuation analysis (DFA) and power spectral analysis (PSA), the paper deals with the characteristics of long-range correlations of colonic pressure activities and their surrogate data of healthy subjects. The DFA is a period representation of such correlations, whereas the PSA is a frequency representation. The analysis of the two methods consistently shows that there are long memories in colonic pressure activities and there exist long-range correlations, whereas there are no such long memories and long-range correlations in the surrogate data, whose sequential orders are randomly shuffled. The results to some extent indicate that human colonic pressure activities with normal motilities do have long memories, but these memories may be weaker or may even be destroyed with dysmotilities.
Adult ; Colon ; physiology ; Data Interpretation, Statistical ; Female ; Humans ; Male ; Middle Aged ; Pressure ; Signal Processing, Computer-Assisted