1. HPLC-DAD analysis and antifungal effect of Hyptis martiusii Benth (Lamiaceae) against Candida strains
Janaina Esmeraldo ROCHA ; Henrique Douglas Melo COUTINHO ; Cicero Roberto Nascimento SARAIVA ; Jaime RIBEIRO-FILHO ; Aracelio Viana COLARES ; Antonia Thassya Lucas Dos SANTOS ; Antonio Judson Targino MACHADO ; Joao Tavares Calixto JUNIOR ; Irwin Rose Alencar MENEZES ; Jose Galberto Martins Da COSTA
Asian Pacific Journal of Tropical Biomedicine 2019;9(3):123-128
Objective: To evaluate the anti Candida activity of Hyptis martiusii decoction and its major compound, caffeic acid alone or in the presence of fluconazole, as well as their cytotoxic effect. Methods: The decoction was characterized using high performance liquid chromatography coupled with diode array detector. For the antifungal activity, the minimum inhibitory concentration (MIC) and the potential effect of the decoction with the fluconazole were evaluated by microdilution method using 96-well microtiter trays. The osmotic fragility test was performed using erythrocytes under saline stress. All tests were performed in triplicate. Results: The chemical characterization of the decoction was performed by high performance liquid chromatography and revealed the presence of seven compounds, including caffeic acid as major constituent. The antifungal tests demonstrated that both decoction (DHm) and caffeic acid obtained from Hyptis martiusii presented MIC and MFC ≥ 4096 μg/mL against Candida albicans and Candida tropicalis strains. However, in the presence of fluconazole, DHm and caffeic acid presented IC
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