Authors made a clinical analysis on 17 patients who were reconstructed with autogenous cranioplasty using refrigerated bone flap at the Department of Neurosurgery, Yonsei University College of Medicine from March 1982 to April 1984, and the results were summarized as follows; 1) The use of refrigerated autogenous bone flap for cranioplasty provided best cosmetic appearance due to natural contour and early operation within 3 months. 2) This procedure was simple with reduction of operating time. No infection nor foreign body reactions were found. 3) There were psychological benefits due to use of autogenous bone flap and radiodensity was appropriate for diagnosis and treatment. Cranioplasty using refrigerated bone flap was thought to be safe and ideal procedure.
Bone Banks ; Diagnosis ; Foreign Bodies ; Humans ; Neurosurgery

BACKGROUND: V. parahaemolyticus, an important seafood-borne pathogen, is the causative agent of gastroenteritis in humans. In this study, the occurrence and distribution of the thermostable hemolysin (tdh) gene and the tdh-related hemolysin (trh) gene were investigated, and as many as 72 different O:K serovar combinations were determined from diarrheal patients and environmental sources in Gyeongbuk provinceas during the 2000-2001. METHODS: A total of 50 strains of V. parahaemolyticus isolated from diarrheal patients and environmental sources were analyzed for the tdh gene and trh gene. The strains were analyzed for kanagawa phenomenon (KP) with the wagatsuma blood agar medium test. Furthermore, the strains belonging to O and K serotypes were determined. DNA sequence determination of the tdh gene were verified using GenBank and analyzed. RESULTS: In the KP experiments of V. parahaemolyticus, 80% of the strains isolated from diarrheal patients showed positive, but all of the strains isolated from environment sources were negative The distribution of O and K serotypes were O3:K29, O3:K6, and O3:K31, atc. isolated from diarrheal patients, and O2:K28, O3:K29, and 04:K34 atc. isolated from environmental sources. As for the tdh and trh gene of V. parahaemolyticus, the tdh gene was detected from 88.0% of diarrheal patients and 16.0% of environmental sources. The trh gene was not detected from diarrheal patients and 8.0 % of environmental sources, whereas all urease positive strains possessed the trh gene. The DNA sequence of tdh gene were verified using GenBank and analyzed the difference, tdh nucleotide sequence was found among the V. parahaemolyticus. CONCLUSION: V. parahaemolyticus is distributed with high frequency in the environmental sources such as sea water, fishes and shellfishes sampled at the Gyeongbuk coastal area, and the O3:K6 strains producing TDH were confirmed, which is prevalent throughout the world, from diarrheal patients. Thus, it is very important to establish a measure to prevent food poisoning by V. parahaemolyticus.
Agar ; Base Sequence ; Databases, Nucleic Acid ; Fishes ; Foodborne Diseases ; Gastroenteritis ; Gyeongsangbuk-do* ; Humans ; Seawater ; Sequence Analysis, DNA ; Shellfish ; Urease ; Vibrio parahaemolyticus* ; Vibrio* ; Virulence Factors* ; Virulence*

Restriction enzyme-mediated integration (REMI) was used to transform uracil auxotrophs of Pleurotus ostreatus to prototrophy. When protoplasts of Pleurotus ostreatus were treated by the reaction mixture containing 10 units of BamHI, the frequency of REMI was about 64 transformants per 1 microg of DNA. This efficiency was increased by 14.2 times compared with that of the conventional PEG transformation. The optimal condition for REMI of P. ostreatus was achieved when 1 microg of linearized pTRura3-2 DNA was added into 1x10(7) protoplasts along with 10 units BamHI. Southern blot analysis revealed that about 50% of transformants examined were caused by REMI event and 30% carried single copy insertion at the genome. This suggested that the REMI method might be a useful tool for efficient transformation and tagging mutagenesis of P. ostreatus.
Blotting, Southern ; DNA ; Genome ; Mutagenesis ; Pleurotus* ; Protoplasts ; Uracil

BACKGROUND: V. parahaemolyticus, an important seafood-borne pathogen, is the causative agent of gastroenteritis in humans. In this study, the occurrence and distribution of the thermostable hemolysin (tdh) gene and the tdh-related hemolysin (trh) gene were investigated, and as many as 72 different O:K serovar combinations were determined from diarrheal patients and environmental sources in Gyeongbuk provinceas during the 2000-2001. METHODS: A total of 50 strains of V. parahaemolyticus isolated from diarrheal patients and environmental sources were analyzed for the tdh gene and trh gene. The strains were analyzed for kanagawa phenomenon (KP) with the wagatsuma blood agar medium test. Furthermore, the strains belonging to O and K serotypes were determined. DNA sequence determination of the tdh gene were verified using GenBank and analyzed. RESULTS: In the KP experiments of V. parahaemolyticus, 80% of the strains isolated from diarrheal patients showed positive, but all of the strains isolated from environment sources were negative The distribution of O and K serotypes were O3:K29, O3:K6, and O3:K31, atc. isolated from diarrheal patients, and O2:K28, O3:K29, and 04:K34 atc. isolated from environmental sources. As for the tdh and trh gene of V. parahaemolyticus, the tdh gene was detected from 88.0% of diarrheal patients and 16.0% of environmental sources. The trh gene was not detected from diarrheal patients and 8.0 % of environmental sources, whereas all urease positive strains possessed the trh gene. The DNA sequence of tdh gene were verified using GenBank and analyzed the difference, tdh nucleotide sequence was found among the V. parahaemolyticus. CONCLUSION: V. parahaemolyticus is distributed with high frequency in the environmental sources such as sea water, fishes and shellfishes sampled at the Gyeongbuk coastal area, and the O3:K6 strains producing TDH were confirmed, which is prevalent throughout the world, from diarrheal patients. Thus, it is very important to establish a measure to prevent food poisoning by V. parahaemolyticus.
Agar ; Base Sequence ; Databases, Nucleic Acid ; Fishes ; Foodborne Diseases ; Gastroenteritis ; Gyeongsangbuk-do* ; Humans ; Seawater ; Sequence Analysis, DNA ; Shellfish ; Urease ; Vibrio parahaemolyticus* ; Vibrio* ; Virulence Factors* ; Virulence*

No abstract available.
Glutathione* ; Oligodendroglia*

We report a case in whom there was right ventricular outflow tract obstruction by extended metastasis from esophageal cancer. A 65-year-old man was admitted to hospital for evaluation of recent onset of weight loss of recent onset and a heart murmur. Physical examination revealed a regular heart rate of 62 beats per minute and a blood pressure of 110/70 mmHg. On cardiac auscultation, a grade 4/6 systolic murmur was heard over the area of pulmonic valve. Electrocardiography showed low voltage. Chest radiography showed a normal cardiac configuration and no pulmonary abnormality was seen. Esophagogram and endoscopy showed a 10cm sized ulcerative and infiltrative esophageal cancer. This esophageal cancer was histologically proven to be a squamous cell carcinoma. To assess the cardiac metastasis, echocardiography, MRI, coronary angiography, and endomyocardial biopsy were performed. The MRI, echocardiography and right ventriculography revealed a 7 cm sized lobulated mass extending to the right ventricular outflow tract, right ventricle, septum, and anterior wall of the left ventricle. Interestingly, the feeding vessels of the tumor were identified by echocardiography and coronary angiography. Histologically, the cardiac tumor was proven to be have the same pathologic findings as the an esophageal cancer, compatible with carcinomatous metastasis.
Aged ; Biopsy ; Blood Pressure ; Carcinoma, Squamous Cell ; Coronary Angiography ; Echocardiography ; Electrocardiography ; Endoscopy ; Esophageal Neoplasms* ; Heart Auscultation ; Heart Murmurs ; Heart Neoplasms ; Heart Rate ; Heart Ventricles ; Humans ; Magnetic Resonance Imaging ; Neoplasm Metastasis* ; Physical Examination ; Radiography ; Systolic Murmurs ; Thorax ; Ulcer ; Weight Loss

In generalized amyloidosis, involvement of heart is not rare, but development fo severe conduction distubance in cardiac amyloidosis is a rare presentation in a clinical situation. We report the first case in Korea of cardiac amyloidosis with complete atrioventricular block, which was confirmed by endomyocardial biopsy. A 59-year-old woman was admitted due to drowsy mentality. We observed a severe atrioventricular conduction disturbance on the electrocardiogram and a marked hypertrophied ventricular wall and a granular sparkling appearance within the wall, a characteristic feature in cardiac infiltration of amyloidosis, on the echocardiography. Endomyocardial biopsy was performed and revealed extensive deposition of amorphous eosinophilic materials within the cardiac muscle cells. Polarizing illumination after Congo-red staining showed typical green birefrigence. We performed a insertion of permanent pacemaker(VVI type) for the treatment of complete atrioventricular block.
Amyloidosis* ; Atrioventricular Block* ; Biopsy ; Echocardiography ; Electrocardiography ; Eosinophils ; Female ; Heart ; Humans ; Korea ; Lighting ; Middle Aged ; Myocytes, Cardiac

Background: and Purpose We aimed to develop a model predicting early recanalization after intravenous tissue plasminogen activator (t-PA) treatment in large-vessel occlusion. Methods: Using data from two different multicenter prospective cohorts, we determined the factors associated with early recanalization immediately after t-PA in stroke patients with large-vessel occlusion, and developed and validated a prediction model for early recanalization. Clot volume was semiautomatically measured on thin-section computed tomography using software, and the degree of collaterals was determined using the Tan score. Follow-up angiographic studies were performed immediately after t-PA treatment to assess early recanalization. Results: Early recanalization, assessed 61.0±44.7 minutes after t-PA bolus, was achieved in 15.5% (15/97) in the derivation cohort and in 10.5% (8/76) in the validation cohort. Clot volume (odds ratio [OR], 0.979; 95% confidence interval [CI], 0.961 to 0.997; P=0.020) and good collaterals (OR, 6.129; 95% CI, 1.592 to 23.594; P=0.008) were significant factors associated with early recanalization. The area under the curve (AUC) of the model including clot volume was 0.819 (95% CI, 0.720 to 0.917) and 0.842 (95% CI, 0.746 to 0.938) in the derivation and validation cohorts, respectively. The AUC improved when good collaterals were added (derivation cohort: AUC, 0.876; 95% CI, 0.802 to 0.950; P=0.164; validation cohort: AUC, 0.949; 95% CI, 0.886 to 1.000; P=0.036). The integrated discrimination improvement also showed significantly improved prediction (0.097; 95% CI, 0.009 to 0.185; P=0.032). Conclusions The model using clot volume and collaterals predicted early recanalization after intravenous t-PA and had a high performance. This model may aid in determining the recanalization treatment strategy in stroke patients with large-vessel occlusion.

Background: and Purpose We aimed to develop a model predicting early recanalization after intravenous tissue plasminogen activator (t-PA) treatment in large-vessel occlusion. Methods: Using data from two different multicenter prospective cohorts, we determined the factors associated with early recanalization immediately after t-PA in stroke patients with large-vessel occlusion, and developed and validated a prediction model for early recanalization. Clot volume was semiautomatically measured on thin-section computed tomography using software, and the degree of collaterals was determined using the Tan score. Follow-up angiographic studies were performed immediately after t-PA treatment to assess early recanalization. Results: Early recanalization, assessed 61.0±44.7 minutes after t-PA bolus, was achieved in 15.5% (15/97) in the derivation cohort and in 10.5% (8/76) in the validation cohort. Clot volume (odds ratio [OR], 0.979; 95% confidence interval [CI], 0.961 to 0.997; P=0.020) and good collaterals (OR, 6.129; 95% CI, 1.592 to 23.594; P=0.008) were significant factors associated with early recanalization. The area under the curve (AUC) of the model including clot volume was 0.819 (95% CI, 0.720 to 0.917) and 0.842 (95% CI, 0.746 to 0.938) in the derivation and validation cohorts, respectively. The AUC improved when good collaterals were added (derivation cohort: AUC, 0.876; 95% CI, 0.802 to 0.950; P=0.164; validation cohort: AUC, 0.949; 95% CI, 0.886 to 1.000; P=0.036). The integrated discrimination improvement also showed significantly improved prediction (0.097; 95% CI, 0.009 to 0.185; P=0.032). Conclusions The model using clot volume and collaterals predicted early recanalization after intravenous t-PA and had a high performance. This model may aid in determining the recanalization treatment strategy in stroke patients with large-vessel occlusion.