Autoradiographic study was performed in order to know the distribution of exogenous radioactive substance, (3)H-6-Thymidine, by liver fluke, Fasciola hepatica. The worms were maintained in vitro for 1 hour, at 37 degrees C, in Tyrode solution containing (3)H-6-Thymidine. After the incubation, the worms were sectioned and autoradiographed by applying the techniques of microautoradiography. The black grains derived from labeled substance were highly accumulated in the vitelline duct and vitelline glands. Moderate amount of blackening showed in the subcuticular muscle fibers, testes, ovary, cirrus sac, oral sucker and pharynx. Slightly labeled particles were in the reticular tissue, ventral sucker, intestinal epithelium, prostate, uterus and ova. There can hardly be found the black grains in the cuticle.
parasitology-helminth-trematoda-Fasciola hepatica ; biochemistry ; autoradiography ; (3)H-6-Thymidine

No abstract available.

No abstract available.

Clinically, benign juvenile melanoma (BJM) is a benign nevus-cell tumor and arise predominantly in children. The lesion usually is solitary and is encountered most commonly on the face and extremities. In most mstances the lesion consists of a dome-shaped, srnall nodule. The histoIogic picture often closely resembles that of a maIignant melanoma and there is no doubt that prior to its recognition as an entity by Spitz in 1948 many cases were misdiagnosed as malignant melanoma. A case of benign juvenile melanoma in 23-year old male is described with brief review of literatures. Hisr.ologic pictures show considerable junctional activity. In the upper dermis edema and ectasia of the various vascular elements are present. The re:us cells are pleomorphic. They are mostly spindle-shaped and are arranged mostly in fairly well circumscribed nests. Giant cells are frequentIy observed. Melanin is scanty and mitotic figures are absent. These are typical histologic pictures of the BJM, To our knowledge, there have been no prior repots of BJM in Korea, based on our own review.
Child ; Dermis ; Dilatation, Pathologic ; Edema ; Extremities ; Giant Cells ; Humans ; Korea ; Male ; Melanins ; Melanoma* ; Young Adult

The warts are benign skin tumors caused by virus, most frequently seen in childhood, and have atendency to regress before reaching adulthood. Various articles have been suggested that wart involution may be involved with immune response of the host, but definite mechanism has not been found. The author studied the tuberculin sensitivities of the wart patients in order to evaluate immune responses of the hosts, and observed the course of the warts after BCG vaccination to the patients for the facilitation of nonspecific immune reaction of the hosts. The results are as follows; 1. Tuberculin positivity was not decreased, however the sensitivity to the tuberculin was decreased among the wart patients than the non-wart groups. 2. No significant difference was observed between the numbers of wart and the degrees of tuberculin sensitivity. R. BCG vaccination was promoted the invotution of warts without valuable changes of the tuberculin sensitivities.
Humans ; Mycobacterium bovis* ; Skin ; Tuberculin* ; Vaccination* ; Warts*

T.E,N. is characterized by large flaccid bullous eruption that has the appearance of the scalded lesion and has large sheets of the pilling off skin. This 49-year-old female patient was suffered from the erythematous skin lesions on the face and. upper chest since 8 hours after oral administration of sulfa drug for endametritis. These lesions spread to the about 80% of whole body surface, and changes to the characterastic flaccid bullous scalded lesion, within next 24 hours. This patient was treated with a massive systemic corticosteroid and antibiotics and etc,so complete recovery of the lesions were found in three case of T.E.N. due to sulfa drug is presented with brief review of literature.
Administration, Oral ; Anti-Bacterial Agents ; Female ; Humans ; Middle Aged ; Skin ; Stevens-Johnson Syndrome* ; Thorax

In order to assess the difference of healing of the medial collateral ligament (MCL) of the knee according to the treatment methods, in vivo animal experiment using a rabbit model was perfoimed. Thirty six MCLs in eighteen rabbits were surgically transected at the mid-portion and treatm nt was done with two different methods, e.g. immobilization wiih plaster cast in right knees (Group I ), and non-immohilization in left knees (Group 2 ). Five rabhits were used as normal controis. To evaluate the biomechanical property of the femur-MCL-tibia complex (FMTC) in time progression, tensile stress tests using material testing machine (Model 8511, Instron company, 1993) were done at 6, 10, l2 weeks postoperatively and the results were as follows. 1. The ultimate load and stiffness (structural property) of the FMTC increased with time in both groups, but group ][ showed significantly higher values than group I . 2. The material property of the MCL checked by stress-strain curve showed higher Young s modulus in group ][ than in group ] . As a results, joint motion seems to improve the biomechanical propeity of the healed MCL.
Animal Experimentation ; Casts, Surgical ; Collateral Ligaments* ; Exercise Test ; Immobilization* ; Joints ; Knee ; Ligaments* ; Rabbits*

Penicillin is still the first choice of drug in the treatment of gonorrhea. The ever lowering sensitivity of Neisseria gonorrhoeae to penicillin and the emergence of PPNG (Penicillinase Producing Neisseria Gonorrhoeae) in the Far East and other parts of the world neceessitate reapprisal of treatrnent schedules with penicillin for gonococcal infections. In Korea, no authentical study in this regard has been done recently. The presence of intracellular gram r.egative diplococci in urethral smear as weIl as the presence of oxidase positive typical, round, convex, smooth, glistening, tratmslucent, greyish colonies of 0.5 to 2mm in diameter a 24 hours composed of grarn negative diplococci are used for presump'ive evidence of gonococccal infection 224 male patients with acnte uncomplicated gonococal infections were treated one of following regimens. Regimen A: 4.8mega unit combicillin (3.6mega unit procaine penicillin+l.2 mega unit sodium penicillin) i.m.. preceded by 1gm probenecid p.o., Regirnen B: 4.8 mega unit procaine penicillin i.m. preceded by 1gm probenecid P.O. Regimen A showed 14 failures(11.3% arnong 124. Regimen B showed 19 failures (19%) among 100 It is suggested that in Korea sesnsitivity to penicillin of Yeisseria gonorrhoeae is quite low.
Appointments and Schedules ; Far East ; Gonorrhea ; Humans ; Korea ; Male ; Neisseria ; Neisseria gonorrhoeae ; Oxidoreductases ; Penicillin G Procaine ; Penicillins* ; Probenecid ; Procaine ; Sodium

Five hundred seventeen male patients with gonococcal urethritis at the VD clinic of Chocng-ku Public Health Center between Feb. 27 and Oct. 27, 1984 the were source cf this study. Forty-five of seventynine patients who had practiced cunnilingus were actual subjects of this study. Aeiss.ria gonorrhoeae were cultured from the pharynx of five patients: one was found to be PPNG. All 5 pharyngeal gonorrhea patients were asymptornatic and their throat appeared to be normal, except injection of the pharynx in one patient. Two patients infected by non-PPNG were administered an oral dose of 1. 0 gm probenecid plus 2 5 gm talampicillin and 2. Ogm Kanamycin sulfate, IM and one patient infected by non-PPNG was administred an oral dose of l. Ogm probenecid and, 3i) minutes later, 6. 0 m.u. fortified procaine penicillin G IM. One gatient infected by PPNG was administered an oral dose of 1, 0 gm probenecid and 30 minutes later, 1 vial of sulbactam sodium/ampicillin sodium, IM. All four pharyngeal gonorrgeal patients were cured, One patient was lost from further evaluation, We consider it important to have pharyngeal cultures done on all gonorrheal patients, at least on those who admit having had orogenital contact in recent episode, because pharyngeal gonococci may be the source of disseminated gonococcal disease and in rare circumstances, the source of mfection for sexual partners, and single lose spectinomycin and orally administered penciillin regimens that are effective against anogenital gonorrhea, had been known to have high rates of failure when used in the pharyngeal gonrorhea.
Gonorrhea* ; Humans ; Kanamycin ; Male ; Penicillin G Procaine ; Pharynx ; Probenecid ; Public Health ; Sexual Behavior ; Sexual Partners ; Sodium ; Spectinomycin ; Sulbactam ; Talampicillin ; Urethritis

A 36-year-old man has suffered from multiple, well defined, variable sized, and elevated papulonodular patches with oozing, scale, and crust on the face. He also presented multiple folliculitis-like eruptions on upper chest, back, and both limbs. He had no leukocytosis, but elevated ESR and fever during development of skin lesions. Histologically a dense iaflammatory cell infiltra,tion composed predominantly of polymorphonuclear neutrophils and few lymphoid cells were seen in the dermis and subcutis. The symptoms and signs did not spond to dapsone and tetracycline, but disappeared on orally administered predniselone.
Adult ; Dapsone ; Dermis ; Extremities ; Fever ; Humans ; Leukocytosis ; Lymphocytes ; Neutrophils* ; Skin ; Skin Diseases* ; Sweet Syndrome ; Tetracycline ; Thorax