No abstract available.
Aged* ; Gallstones* ; Humans

A clinical and radiological obsevation was made on 62 cases of urinary tract injury in the Dept. of urology,Chosun Universtiy hospital during the period from January 1979 to December 1983. The results were as follows; 1.Age distribution of the lower urinary tract injury was the highest in the age 41 to 50(39.6%). The sex ratio, maleto female was 6.8:1. 2. The most common cause of the L.U.T.I. was traffic accident in 23 cases (37.1%) and othersare fall down in 15 cases (24.2%), blunt trauma in 13 cases (21.0%), saddle injury in 8 cases(12.9%). 3. Theradiological finding of the bladder injury shows intraperitoneal extravasation in 11 caess(64.7%), extraperitonealextravasation in 5 cases(29.4%), and deformity of bladder in 12 cases(70.6%). 4. The radiological finding of theurethral injury shows extravasation of contrast media in 44 cases(97.8%). venous intravasation in 11 cases(24.4%),penile urethra in 1 case(2.3%). 6. The L.U.T.I. was associated with pelvic bone fracture in 33 cases (53.2%) esp.pubic bone fracture in 24 cases(38.7%). 7. The complication of the L.U.T.I. was urethral stricture in 24cases(38.7%), fistula formation in 3 cases(4.8%), incontinence in 2 cases(3.2%).
Accidents, Traffic ; Congenital Abnormalities ; Extravasation of Diagnostic and Therapeutic Materials ; Female ; Fistula ; Fractures, Bone ; Humans ; Pelvic Bones ; Sex Ratio ; Urethra ; Urethral Stricture ; Urinary Bladder ; Urinary Tract

PURPOSE: This study was designed to find out whether protein kinase C (PKC) may affect telomerase activity in human ovarian cancers. MATERIALS AND METHODS: To determine whether PKC modulators influence PKC activities, NIH: OVCAR-3 and CUMO-2, cells were treated with PKC inhibitors, G 6976 and bisindolyl maleimide I, and PKC activator, 12-O-tetradecanoyl phorbol 13-acetate (TPA). Telomerase acti vity was determined by telomeric repeat amplification protocol (TRAP). Analysis of the expres sion of each telomerase subunits, human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT), was performed by RT-PCR. We also examined the alternative splicing of hTERT. RESULTS: G 6976 and bisindolylmaleimide I inhibited PKC activity. Telomerase activities appeared to be affected in a time-dependent manner by these two PKC inhibitors. PKC activities were increased in parallel with telomerase activity by TPA at the low dose (10 nM), but their activities were down-regulated at the high dose (1 micrometer). RT-PCR demonstrated the presence of hTR and hTERT mRNA before and after the treatment of PKC modulators, respectively, and showed the presence of one alternatively spliced transcript and full-length hTERT transcripts. CONCLUSION: These results showed that telomerase activity was affected by PKC and suggested PKC modulation may serve as an useful tool in the regulation of telomerase activity.
Alternative Splicing ; Cell Line* ; Humans* ; Ovarian Neoplasms* ; Protein Kinase C* ; Protein Kinases* ; RNA ; RNA, Messenger ; Telomerase* ; Tetradecanoylphorbol Acetate

No abstract available.
Fruit* ; Pregnancy*

We have experienced six cases of postoperative capsular bag distension following continuous curvilinear capsulorhexis(CCC) and phacoemulisification with posterior chamber intraocular lens. Successful CCC, phacoemulsification and in the bag fixation of intraocular lens(IOL) were accomplished, and the optics of the IOL were made of silicone material in 6 cases. We have observed various degree of anterior chamber shallowing, anterior displacement of IOL, bulging of posterior capsule, and myopic shift of refractive error, and these findings improved by YAG laser anterior or posterior capsulotomy. Mean refractive change was +2.6D(diopter) at post-laser 1 day.
Anterior Chamber ; Cataract* ; Lasers, Solid-State ; Lenses, Intraocular ; Phacoemulsification ; Posterior Capsulotomy ; Refractive Errors ; Silicones

PURPOSE: Pleural effusions are recognised complications of mycoplasmal, tuberculous, and parapneumonic infections. Tuberculosis is still a common infectious disease in Korea, but the difficulty is that this disease is initially difficult to discriminat from common community-acquired pneumonia. It makes immediate diagnosis and proper treatment difficult. We investigate the common characteristics of pleural fluid and blood in mycoplasmal and tuberculous pleural effusions. METHODS: A retrospective clinical study was performed with four different patients groups. A total of 70 patients with pleural effusions were included: 7 with tuberculous pleural effusions, 34 with mycoplasmal pleural effusions, 8 with malignant pleural effusions, and 21 with other infectious pleural effusions. RESULTS: Glucose and pH levels of pleural effusions in other infectious pleural effusions were significantly lower than in the other groups. (P< 0.01) Proportions of lymphocytes of pleural effusions in tuberculous pleural effusions were significantly higher than in the other groups. (P< 0.01) ADA levels of pleural effusions were not statistically different in the four disease groups. (P=0.303) Protein levels of blood in mycoplasmal pleural effusions were significantly lower than in the other groups. (P< 0.05) Albumin levels of blood in other infectious pleural effusions were significantly lower than in the other groups. (P< 0.05) LDH levels of blood in tuberculous pleural effusions were significantly higher than in the other groups. (P< 0.05) CONCLUSION: Our results show that ADA levels cannot be very valuable as diagnostic markers of tuberculous pleural effusions. More prospective and serial studies combined with PPD skin tests are required to prove correct and rapid diagnoses of tuberculous pleural effusions.
Communicable Diseases ; Diagnosis ; Glucose ; Humans ; Hydrogen-Ion Concentration ; Korea ; Lymphocytes ; Mycoplasma ; Pleural Effusion* ; Pleural Effusion, Malignant ; Pneumonia ; Retrospective Studies ; Skin Tests ; Tuberculosis

OBJECTIVE: Flavopiridol that inhibits cyclin-dependent kinase, can cause cell cycle arrest, induce apoptosis in human tumor cell lines. In the present study, we investigated apoptotic effects of flavopiridol and the underlying molecular mechanisms in human ovarian cancer cell lines. METHODS: We used TOV-21G and TOV-112D cell lines. The cell viability was tested by MTT assay and apoptosis was assessed by TUNEL assay and annexin-V binding. Western blot was used to examine apoptosis related protein levels. MAP kinase activity was analyzed by non-radioactive MAP kinase assay kit. RESULTS: Treatment of TOV-21G and TOV-112D cells with flavopiridol (50 nM to 1000 nM) led to a dose- and time-dependent inhibition of cell growth and survival. Dose-related induction of apoptosis was also observed in these cell lines. Flavopiridol (500 nM) induced striking decreases in the levels of the antiapoptic proteins Mcl-1, Bcl-X(L), and XIAP in both cell lines. In contrast, expression of Bax, Bcl-2, and AIF was not significantly influenced by flavopiridol. Although flavopiridol resulted in accumulation of p53 in both cells, flavopiridol mediated apoptosis was p53 independent because it occurred to the same degree in TOV-112D cells in which p53 was inactivated by mutation. Flavopiridol treatment resulted in enhanced cleavage of pro-caspase 9 and activation of caspase 3. Apoptosis was associated with suppression of ERK activity. CONCLUSION: Although the precise mechanisms of flavopiridol mediated cytotoxicity have not been fully defined, these data suggest that flavopiridol has activity against ovarian cancers in vitro and is worthy of continued clinical development in the treatment of ovarian cancer.
Apoptosis ; Blotting, Western ; Caspase 3 ; Cell Cycle Checkpoints ; Cell Line ; Cell Line, Tumor ; Cell Survival ; Flavonoids ; Humans ; In Situ Nick-End Labeling ; Ovarian Neoplasms ; Phosphotransferases ; Piperidines ; Proteins ; Strikes, Employee

To date, various clinical procedures have been used to restore periodontal apparatus destroyed by periodontal disease. And then, many experimental approaches have been proceeded to develop treatment methods to promote periodontal regeneration. Mechanical, chemical treatments to enhance the attachment of periodontal tissue cells as changing the physical properties of root surfaces, bone graft procedure, and treatments for guided tissue regeneration have been used for periodontal regeneration. However, recent studies have revealed that biologic factors such as growth factors promote biologic mechanism associated with periodontal regeneration. This study was done to enucleate how ELF stimulus affect the periodontal regeneration. We can have following conclusions from this experimental results. The influence of low frequency(ELF) electric stimulus (30HZ at 10micronA) known to promote bone formation in vivo, was evaluated for its ability to affect bone cell function in vitro. After 12 hour exposure of ELF stimulus at most appropriate densities (5x10(4) cells/cm2) to increase osteoblastic cells normally, rat calvarial cells were incubated for 60 hours were used in this study. We have found ELF stimulus suppress calvarial cell proliferation and the ability of protein synthesis, enhance the alkaline phosphatase activity significantly.
Alkaline Phosphatase ; Animals ; Biological Factors ; Cell Proliferation ; Guided Tissue Regeneration ; Intercellular Signaling Peptides and Proteins ; Osteoblasts ; Osteogenesis ; Periodontal Diseases ; Rats ; Regeneration ; Skull* ; Transplants

Viral infection induces numerous tripartite motif (TRIM) proteins to control antiviral immune signaling and viral replication. Particularly, SPRY-containing TRIM proteins are found only in vertebrates and they control target protein degradation by their RING-finger and SPRY domains, and proper cytoplasmic localization. To understand TRIM30 function, we analyzed its localization pattern and putative roles of its RING-finger and SPRY domains. We found that TRIM30 is located in actin-mediated cytoplasmic bodies and produces colocalized ubiquitin chains in SPRY domain- and RING-finger domain-dependent ways that are degraded by autophagy and the proteasome. These results suggest a TRIM protein-dependent degradation mechanism by cytoplasmic body formation with actin networks.
Amino Acid Sequence ; Animals ; Autophagy ; Cell Line ; Inclusion Bodies/*metabolism ; Intracellular Signaling Peptides and Proteins/chemistry/genetics/*metabolism ; Mice ; Molecular Sequence Data ; Polyubiquitin/*metabolism ; Proteasome Endopeptidase Complex/metabolism ; Protein Interaction Domains and Motifs ; Protein Transport ; Proteolysis ; RING Finger Domains

Nervous wreck (Nwk), a protein that is present at Type 1 glutamatergic synapses that contains an SH3 domain and an FCH motif, is a Drosophila homolog of the human srGAP3/MEGAP protein, which is associated with mental retardation. Confocal microscopy revealed that circles in Nwk reticulum enclosed T-shaped active zones (T-AZs) and partially colocalized with synaptic vesicle (SV) markers and both exocytosis and endocytosis components. Results from an electron microscopic (EM) analysis showed that Nwk proteins localized at synaptic edges and in SV pools. Both the synaptic areas and the number of SVs in the readily releasable (RRPs) and reserve (RPs) SV pools in nwk2 were significantly reduced. Synergistic, morphological phenotypes observed from eag¹;nwk² neuromuscular junctions suggested that Nwk may regulate synaptic plasticity differently from activity-dependent Hebbian plasticity. Although the synaptic areas in eag¹;nwk² boutons were not significantly different from those of nwk², the number of SVs in the RRPs was similar to those of Canton-S. In addition, three-dimensional, high-voltage EM tomographic analysis demonstrated that significantly fewer enlarged SVs were present in nwk² RRPs. Furthermore, Nwk formed protein complexes with Drosophila Synapsin and Synaptotagmin 1 (DSypt1). Taken together, these findings suggest that Nwk is able to maintain synaptic architecture and both SV size and distribution at T-AZs by interacting with Synapsin and DSypt1.