1.A Case of Lecalized Cutis Laxa.
Korean Journal of Dermatology 1970;8(2):87-90
Cutis laxa is a rarely discussed entity which manifests itself primarily as loose redundant skin and may be accompanied by internal anomalies. Localized cutis laxa is involved to the ear lobules, eyelids an lips in 34 years old male was described. Diagnosis of localized cutis laxa was established by clinical characteristic manifestations and histopathological studies.
Adult
;
Cutis Laxa*
;
Diagnosis
;
Ear
;
Eyelids
;
Humans
;
Lip
;
Male
;
Skin
2.Effect of Imipramine on the Contractility of Single Cells Isolated for Canine Detrusor.
Chan Wook HUH ; Kang Youn LEE ; Won Joon KIM
Yeungnam University Journal of Medicine 1994;11(2):293-302
The objective of this study was to establish a good methodology to isolate single smooth muscle cells that are alive and respond properly to pharmacological agents. Canine urinary bladders were employed as the source of single cells, and acetylcholine, atropine and imipramine were used as indicators of pharmacological responsiveness. Imipramine, an antidepressant drug exhibited the anticholinergic and calcium antagonizing properties on rat detrusor muscle. To establish a control value for a further experiment to elucidate the mechanism of action of imipramine on detrusor muscle, we measured the concentration-response of single cells to acetylcholine in the presesnce of imipramine by length of the cells and compared the result with the response in the presence of atropine. Tiny chops of smooth muscle taken from anesthetized canine urinary bladder were incubated in collagenase solution at 36℃ for 17-20 minutes. The collagenase solution included collagenase 1.2 mg/ml, soybean tryspin inhibitory 0.08 mg/ml, bovine serum albumin 2% in 10 ml Krebs-Henseleit buffer solution aerated with a consistent breeze of 95/5% O2/CO2 to maintain the pH at 7.4. After washing with plain K-H solution on 450 mesh, cells were dissociated from the digested tissue for 12-15 minutes. Cell suspension was transfered in 5 ml test tubes and acetylcholine was added for the final concentration to be 10⁻¹⁴~10⁻⁹M. To find the optimal time to fix the cells to determine the contractile responses, 1% acrolein was added 5, 10, 20, 30, 60 and 120 seconds after the administration of ACh. The length of cells fixed by acrolein were measured by microscaler vis CCTV camers on phaes-contrast microscope. The average length of 50 cells from a slide glass was taken as the value of a sample at the very concentration point. Single cells were isolated from canine detrusor. The length of untreated cells varied from 82 µm to 94 µm. The maximal response to actylcholine 10E-9M was accomplished within 5 seconds of exposure, and the shortening was 19±3%. Atropine reduced the contraction of the cells concentration-dependently. Lmipramine which exerts a cholinergic blocking action on some smooth muscles also reduced the contraction concentration-dependently and by a similar pattern as atropine. These findings document that imipramine may exerts a cholinergic blocking activity in the single smooth muscle cells isolated from canine urinary bladder.
Acetylcholine
;
Acrolein
;
Animals
;
Atropine
;
Calcium
;
Collagenases
;
Glass
;
Hydrogen-Ion Concentration
;
Imipramine*
;
Muscle, Smooth
;
Myocytes, Smooth Muscle
;
Rats
;
Serum Albumin, Bovine
;
Soybeans
;
Urinary Bladder
3.Merkel Cell Carcinoma Coexistent with Epidermal Cyst in an Immunosuppressed Man.
Young In JEONG ; Joon Won HUH ; Geon KIM ; Eun Jung KIM ; Hyang Joon PARK ; Mihn Sook JUE
Korean Journal of Dermatology 2014;52(5):368-370
No abstract available.
Carcinoma, Merkel Cell*
;
Epidermal Cyst*
;
Immunosuppression
4.Pilomatricoma on the Ear Helix.
Young In JEONG ; Joon Won HUH ; Geon KIM ; Mihn Sook JUE ; Hyang Joon PARK ; Eun Jung KIM
Korean Journal of Dermatology 2015;53(3):261-262
No abstract available.
Ear*
;
Pilomatrixoma*
5.Effect of GABA on the contratility of small intestine isolated from rat.
Joon Young HUH ; Oh Cheol KWON ; Jeoung Hee HA ; Kwang Youn LEE ; Won Joon KIM
Yeungnam University Journal of Medicine 1991;8(2):95-105
This study was designed to investigate the effect of GABA and related substances on the spontaneous contraction of rat small intestine. The rats (Sprague-Dawley), weighing 200-250g, were sacrificed by cervical dislocation, and the small intestine was isolated. Longitudinal muscle strips from duodenum, jejunum and ileum were suspended in Biancani's isolated muscle chambers and myographied isometrically. GABA and muscimol, a GABA A receptor agonist relaxed the duodenum and jejunum significantly, but baclofen-induced relaxation in those muscle strips negligible. The effectiveness of GABA and muscimol in various regions were the greatest on duodenum, and greater on jejunum than on ileum The effect of GABA and muscimol was antagonized by bicuculline, a competitive GABA A receptor antagonist and picrotoxin, a noncompetitive GABA A receptor antagonist. Duodenal relaxation induced by GABA and muscimol was unaffected by hexamethonium, but was prevented by tetrodotoxin. These results suggest that GABA inhibit the contractility of smooth muscle with distinct regional difference of efficacy, and the site of inhibitory action is the GABA A receptor existing at the presynaptic membrane of postganglionic excitatory nerves.
Animals
;
Bicuculline
;
Dislocations
;
Duodenum
;
GABA-A Receptor Agonists
;
GABA-A Receptor Antagonists
;
gamma-Aminobutyric Acid*
;
Hexamethonium
;
Ileum
;
Intestine, Small*
;
Jejunum
;
Membranes
;
Muscimol
;
Muscle, Smooth
;
Picrotoxin
;
Rats*
;
Receptors, GABA-A
;
Relaxation
;
Tetrodotoxin
6.Color Doppler Assessment of Mitral Regurgitation in Childhood.
Jo Sam KOO ; Jae Won HUH ; Yang Dong PARK ; Hyun Kee CHUNG ; Ho Joon KIM
Journal of the Korean Pediatric Society 1989;32(6):773-778
No abstract available.
Mitral Valve Insufficiency*
7.Comment on 'Rhombic Flap Reconstruction'.
Kwang Hyun CHOI ; Joon Won HUH ; Young In JEONG ; Mihn Sook JUE ; Hyangjoon PARK
Korean Journal of Dermatology 2015;53(10):820-822
No abstract available.
9.Photodynamic Therapy with Methyl Aminolevulinate for Disseminated Superficial Porokeratosis.
Joon Won HUH ; Young In JEONG ; Geon KIM ; Mihn Sook JUE ; Eun Jung KIM ; Hyangjoon PARK
Korean Journal of Dermatology 2014;52(10):757-758
No abstract available.
Photochemotherapy*
;
Porokeratosis*
10.A Case of Adult Colloid Milium on the Scalp.
Joon Won HUH ; Young In JEONG ; Geon KIM ; Mihn Sook JUE ; Hyangjoon PARK ; Eun Jung KIM
Korean Journal of Dermatology 2015;53(9):740-741